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In vivo measurement of NADH fluorescence lifetime in skeletal muscle via -ber-coupled time-correlated single photon counting

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Journal of Innovative Optical Health Sciences 2024年第1期17卷 121-134页

作者： Kathryn m.priest Jacob V.Schluns Nathania Nischal colton L.Gattis Jeffrey c.Wolchok Timothy J.muldoon Department of Biomedical Engineering University of Arkansas FayettevilleARUSA

Nicotinamide adenine dinucleotide(NADH)is a cofactor that serves to shuttle electrons during metabolic processes such as glycolysis,the tricarboxylic acid cycle,and oxidative phosphorylation(OXPHOS).NADH is autofluorescent,and itsfluorescence lifetime can be used to infer metabolic dynamics in living ***-coupled time-correlated single photon counting(TcSPc)equipped with an implantable needle probe can be used to measure NADH lifetime in vivo,enabling investigation of changing metabolic demand during muscle contraction or tissue *** study illustrates a proof of concept for point-based,minimally-invasive NADHfluorescence lifetime measurement in *** muscle loss(VmL)injuries were created in the left tibialis anterior(TA)muscle of male Sprague Dawley *** lifetime measurements were collected before,during,and after a 30 s tetanic contraction in the injured and uninjured TA muscles,which was subsequently-t to a biexponential decay model to yield a metric of NADH utilization(cytoplasmic vs protein-bound NADH,the A11/A22 ratio).On average,this ratio was higher during and after contraction in uninjured muscle compared to muscle at rest,suggesting higher levels of free NADH in contracting and recovering muscle,indicating increased rates of *** injured muscle,this ratio was higher than uninjured muscle overall but decreased over time,which is consistent with current knowledge of inflammatory response to injury,suggesting tissue regeneration has *** data suggest that-ber-coupled TcSPc has the potential to measure changes in NADH binding in vivo in a minimally invasive manner that requires further investigation.

关键词： Glycolysis oxidative phosphorylation energy metabolism volumetric muscle loss

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Stomatal dynamics are regulated by leaf hydraulic traits and guard cell anatomy in nine true mangrove species

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Plant Diversity 2024年第3期46卷 395-405页

作者： Ya-Dong qie qi-Wei Zhang Scott A.m.mcAdam Kun-Fang cao State Key Laboratory for conservation and Utilization of Subtropical Agro-Bioresources College of ForestryGuangxi UniversityNanning 530004China Guangxi Key Laboratory of Forest Ecology and conservation College of ForestryGuangxi UniversityNanning 530004China Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection Ministry of EducationGuangxi Normal UniversityGuilin 541001China Guangxi Key Laboratory of Landscape Resources conservation and Sustainable Utilization in Lijiang River Basin Guangxi Normal UniversityGuilin 541001China Purdue center for Plant Biology Department of Botany and Plant PathologyPurdue UniversityWest LafayetteIN 47907USA

Stomatal regulation is critical for mangroves to survive in the hyper-saline intertidal zone where water stress is severe and water availability is highly ***,very little is known about the stomatal sensitivity to vapour pressure deficit(VPD)in mangroves,and its co-ordination with stomatal morphology and leaf hydraulic *** measured the stomatal response to a step increase in VPD in situ,stomatal anatomy,leaf hydraulic vulnerability and pressure-volume traits in nine true mangrove species of five families and collected the data of genome *** aimed to answer two questions:(1)Does stomatal morphology influence stomatal dynamics in response to a high VPD in mangroves?with a consideration of possible influence of genome size on stomatal morphology;and(2)do leaf hydraulic traits influence stomatal sensitivity to VPD in mangroves?We found that the stomata of mangrove plants were highly sensitive to a step rise in VPD and the stomatal responses were directly affected by stomatal anatomy and hydraulic ***,denser stomata was correlated with faster stomatal closure at high VPD across the species of Rhizophoraceae,and stomata size negatively and vein density positively correlated with genome *** negative leaf osmotic pressure at the full turgor(πo)was related to higher operating steady-state stomatal conductance(gs);and a higher leaf capacitance(cleaf)and more embolism resistant leaf xylem were associated with slower stomatal responses to an increase in *** addition,stomatal responsiveness to VPD was indirectly affected by leaf morphological traits,which were affected by site salinity and consequently leaf water *** results demonstrate that mangroves display a unique relationship between genome size,stomatal size and vein packing,and that stomatal responsiveness to VPD is regulated by leaf hydraulic traits and stomatal *** work provides a quantitative framework to better understand of stomatal regulation in mangroves

关键词： Stomatal temporal kinetics Vapour-pressure deficit(VPD) Leaf water relations Leaf hydraulic vulnerability Leaf osmotic potential Genome size

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Transcriptome Analysis of Heterosis in Survival in the Hybrid Progenies of‘Haida No.1’and Orange-Shelled Lines of the Pacific Oyster crassostrea gigas

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Journal of Ocean University of china 2024年第1期23卷 199-208页

作者： YANG Hang LI qi Key Laboratory of mariculture Ministry of EducationOcean University of ChinaQingdao 266003China

Heterosis has been exploited to enhance the yield and adaptability in various shellfish species;however,the molecular basis of it remains *** Pacific oyster crassostrea gigas is one of the most economically important aquaculture species,and its productive traits can be improved by ***,an intraspecific cross between orange shell(O,10th generation)and‘Haida No.1’(H,13th generation)of *** was performed to assess the heterosis of survival *** rates of hybrid family(OH)and inbred families(HH and OO)were compared at larval stage,and eyed-pediveliger larvae of three families were subjected to transcriptome *** analysis results of best-parent heterosis and mid-parent heterosis showed that the hybrid family exhi-bited a high heterosis in survival relative to the parental *** OH-m(OH ***)and OH-P(OH ***)had 425 and 512 dif-ferentially expressed genes(DEGs),*** enrichment analysis of these DEGs revealed that the significantly enrich-ed genes function in virion binding,c-type lectin receptor signaling pathway,cellular defense response and other immune-related pro-cesses,which involves perlucin-like protein,cD209 antigen-like protein,ZNFX1,caspase-3 and acan *** differentially ex-pressed genes in OH-m and OH-P,together with the immune-related processes mentioned above may play an important role in the larval survival of *** addition,three genes(cYP450,fucolectin and perlucin-like)are associated with the orange shell and low survival of maternal oyster *** findings provide support for the application of hybrid with superior survival and will facilitate the understanding of heterosis formation in the Pacific oyster.

关键词： crassostrea gigas survival rate heterosis transcriptome

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All laser direct writing process for temperature sensor based on graphene and silver

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Frontiers of Optoelectronics 2024年第1期17卷 45-55页

作者： qi Li Ruijie Bai Lianbo Guo Yang Gao Shanghai Key Laboratory of Intelligent Sensing and Detection Technology School of Mechanical and Power EngineeringEast China University of Science and TechnologyShanghai 200237China North Automatic control Technology Institute Taiyuan 030006China Wuhan National Laboratory for Optoelectronics Huazhong University of Science&TechnologyWuhan 430074China

A highly sensitive temperature sensing array is prepared by all laser direct writing(LDW)method,using laser induced silver(LIS)as electrodes and laser induced graphene(LIG)as temperature sensing layer.A finite element analysis(FEA)photothermal model incorporating a phase transition mechanism is developed to investigate the relationship between laser parameters and LIG properties,providing guidance for laser processing parameters selection with laser power of 1–5 W and laser scanning speed(greater than 50 mm/s).The deviation of simulation and experimental data for widths and thickness of LIG are less than 5%and 9%,*** electrical properties and temperature responsiveness of LIG are also *** changing the laser process parameters,the thickness of the LIG ablation grooves can be in the range of 30–120μm and the resistivity of LIG can be regulated within the range of 0.031–67.2Ω・*** percentage temperature coefficient of resistance(TcR)is calculated as−0.58%/°***,the FEA photothermal model is studied through experiments and simulations data regarding LIS,and the average deviation between experiment and simulation is less than 5%.The LIS sensing samples have a thickness of about 14μm,an electrical resistivity of 0.0001–100Ω・m is insensitive to temperature and pressure ***,for a LIS-LIG based temperature sensing array,a correction factor is introduced to compensate for the LIG temperature sensing being disturbed by pressure stimuli,the temperature measurement difference is decreased from 11.2 to 2.6°c,indicating good accuracy for temperature measurement.

关键词： Laser direct writing Temperature sensor Finite element analysis Laser induced graphene Laser induced silver

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A robust viscoelastic surfactant tolerating 20% Hcl up to 150℃ for oil well stimulation

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Petroleum Science 2024年第3期21卷 1865-1874页

作者： Ji Wang Ning qi Hong-Yao Yin Yu-Jun Feng Polymer Research Institute State Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengdu610065SichuanPR.China Tianfu Yongxing Laboratory New Theory and Technology of CO_(2) Capture Research CenterChengdu610065SichuanPR China School of Petroleum Engineering China University of Petroleum(East China)Qingdao266580ShandongPR China

Viscoelastic surfactants(VES)are often used as viscous diverters in acidizing stimulation to prolong the acid consumption time and maximize zonal coverage of the acid for improving well ***,the ceiling temperature of commercial VES cannot exceed 120℃in practical use because of the poor thermal stability and fragile molecular structure,hindering their implementation in hightemperature oil reservoirs,i.e.,≥150℃.Here we synthesized a novel c22-tailed diamine,N-erucaminopropyl-N,N-dimethylamine(EDPA),and examined comparatively its rheological behavior,assemblies morphology and molecular stability in 20 wt%Hcl with a commercial VES,erucyl dimethyl amidopropyl betaine(EDAB).The feasibility of EDPA for acidizing stimulation was assessed by acid etching of carbonate rock with its Hcl solution at 150℃.Rheological results showed that the 2.5 wt%EDPA—20 wt%Hcl solution maintains stable viscosity of 90 m Pa s at 150℃for 60 min,while that of 2.0 wt%EDAB Hcl solution is just 1 m Pa s under identical conditions.1H NmR spectra and cryo-TEm observations revealed that the chemical structure and self-assembled architectures of EDPA remained intact in such context,but the EDAB suffered from degradation due to the hydrolysis of the amide group,accounting for the poor heat-resistance and *** reaction rate of 2.5 wt%EDPA Hcl solution with carbonate rock was one order of magnitude lower than that of 20 wt%Hcl solution at 150℃,underpinning the potential of EDPA to be used in the high-temperature reservoirs *** work improved the thermal tolerance of VES in highly concentrated Hcl solution,paving a feasible way for the acidization of high-temperature reservoir environments(~150℃).

关键词： Viscoelastic surfactants Thermal stability Self-diverting acid Wormlike micelles Acidizing stimulation

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Optimization on crystallization process of the magnetic core lysozyme

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Particuology 2024年第4期87卷 106-113页

作者： Lixia Hou chunxiao Qu min Su Zhihan Liang qi Hao School of chemical Engineering Hebei University of TechnologyTianjin300130China

The continuous growth of the biomedical market highlights the significance of improvement in the ef-ficiency of batch production for protein drug ***,we investigated the effect of polymer-modified Fe_(3)O_(4) nanoparticles on lysozyme(Lys)crystallization at different conditions,using function-alized Fe_(3)O_(4) magnetic nanoparticles(Polymer c@Fe_(3)O_(4))as nucleation *** study revealed that the surface charge density of Polymer c@Fe_(3)O_(4) was highest at a modification mass ratio of Fe_(3)O_(4) to Polymer c is 8∶*** ratio facilitates the augmentation of electrostatic interaction and the promotion of *** adding 15%of the Polymerc@Fe_(3)O_(4) prepared from Fe_(3)O_(4) with an average particle size of 150 nm,the magnetic core lysozyme(m-Lys)crystals with an average particle size of 11.08μm,narrow size distribution,and regular morphology were obtained in a yield of 82.42%within 60 min.

关键词： Protein crystallization magnetic particle Lysozyme

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Structure,electronic,and nonlinear optical properties of superalkaline m_(3)O(m=Li,Na)doped cyclo[18]carbon

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chinese Physics B 2024年第2期33卷 311-317页

作者：刘晓东卢其亮罗其全 School of Physics and material Science Anhui UniversityHefei 230601China Institute of Physical Science and Information Technology Anhui UniversityHefei 230601China Department of chemical Physics University of Science and Technology of ChinaHefei 230026China

cyclo[18]carbon has received considerable attention thanks to its novel geometric configuration and special electronic *** have low ionization *** a superalkali in cyclo[18]carbon is an effective method to improve the optical properties of the system because considerable electron transfer *** this paper,the geometry,bonding properties,electronic structure,absorption spectrum,and nonlinear optical(NLO)properties of superalkaline m_(3)O(m=Li,Na)-doped cyclo[18]carbon were studied by using density functional theory.m_(3)O and the c_(18) rings are not *** c_(18) ring still exhibits alternating long and short *** charge transfer between m_(3)O and c_(18) forms stable[m_(3)O]+[c_(18)]-ionic complexes.c_(18)m_(3)O(m=Li,Na)shows striking optical nonlinearity,i.e.,their first-and second-order hyperpolarizability(βvec andγ||)increase considerably atλ=1907 nm and 1460 nm.

关键词： superalkaline doped cyclocarbon structure and electronic properties nonlinear optical properties density functional theory(DFT)

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Jianpi Decoction combined with medroxyprogesterone Acetate Alleviates cancer cachexia and Prevents muscle Atrophy by Directly Inhibiting E3 Ubiquitin Ligase

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chinese Journal of Integrative medicine 2024年第6期30卷 499-506页

作者： LI qi KONG Zhao-di WANG Huan GU Hong-hui cHEN Zhong Li Shi-guang cHEN Yi-qi cAI Yu YANG Zhen-jiang Department of Oncology and Hematology Shenzhen Traditional Chinese Medicine HospitalShenzhenGuangdong Province518033China college of Pharmacy/International cooperative Laboratory of Traditional chinese medicine modernization and Innovative Drug Development of ministry of Education(mOE)of china Jinan UniversityGuangzhou510632China Guangdong Key Lab of Traditional chinese medicine Information Technology Jinan UniversityGuangzhou510632China

ObjectiveTo provide comprehensive evidence for the anti-cancer cachexia effect of Jianpi Decoction(JP)and to explore its mechanism of anti-cancer *** mouse model of colon cancer(cT26)-induced cancer cachexia(cc)was used to investigate the anti-cc effect of JP combined with medroxyprogesterone acetate(mPA).Thirty-six mice were equally divided into 6 groups:normal control,cc,mPA(100 mg·kg^(−1)·d^(−1)),mPA+low-dose(20 mg·kg^(−1)·d^(−1))JP(L-JP),mPA+medium-dose(30 mg·kg^(−1)·d^(−1))JP(m-JP),and mPA+high-dose(40 mg·kg^(−1)·d^(−1))JP(H-JP)*** successful modeling,the mice were administered by gavage for 11 *** body weight and tumor volume were measured and recorded every 2 d starting on the 8th day after *** liver,heart,spleen,lung,kidney,tumor and gastrocnemius muscle of mice were collected and *** pathological changes of the tumor was observed,and the cross-sectional area of the gastrocnemius muscle was *** protein expressions of STAT3 and E3 ubiquitinase in the gastrocnemius muscle were measured by Western *** addition,an in vitro c2c12 myotube formation model was established to investigate the role of JP in hindering dexamethasone-induced muscle *** vitro experiments were divided into control,model,and JP serum *** 2-d administration,microscopic photographs were taken and myotube diameters were *** blot was performed to measure the protein expressions of STAT3 and E3 *** combined with mPA restored tumor-induced weight loss(Pmuscle fiber size(Pmechanistically,JP reduced the expression of atrophy-related proteins muRF1 and mAFbx in tumor-induced muscle atrophy in vivo(Pced the expression of atrophy-related proteins muRF1 and mAFbx and p-STAT3 phosphorylation(Pc2c12 myotubes treated with dexamethasone in *** of JP combined with mPA restores t

关键词： cancer cachexia muscle atrophy Jianpi Decoction chinese medicine E3 ubiquitin ligases

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Effect of Preload Force on Heat Generation of Li(Ni_(0.8)co_(0.1)mn_(0.1))O_(2)/SiO_(x)-c System Batteries:The Discharge Process

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Journal of Thermal Science 2024年第5期33卷 1809-1825页

作者： LIN chunjing LAI Zhenxing BAI Guangli mA Biao WEI Zhen qi chuang LIU Shiqiang Key Laboratory of Advanced manufacturing Technology for Automobile Parts Ministry of EducationChongqing University of TechnologyChongqing 400054China china Auto Research New Energy Vehicle Inspection center(Tianjin)co Tianjin 300300China

Lithium-ion batteries(LIBs)undergo various degradation phenomena such as material decomposition,structural change and uneven lithium ion distribution during long-term cycles,which would affect their performance and *** order to improve the performance of the LIBs during their life cycle,preload force is preset when the batteries are *** preload forces will in turn affect the cycle life and heat generation of the *** order to address this issue,this work carries out charge/discharge cycle tests on a Ncm811 battery under different preload *** calorimetry tests are performed to investigate the battery heat generation under different states of health(SOHs)and preload *** on the test results,an empirical prediction model for heat generation power as a function of SOH is *** show that when the preload force is 5 N·m,the battery capacity decreases in the slowest rate and the average heat generation power is the *** in peaks of the incremental capacity curve can be used to characterize the loss of lithium at the electrode,which in turn characterizes the change of heat generation power of the *** average heat generation power is mainly affected by the SOH,going through a period of trough with the decrease of the SOH and continuing to increase after crossing the critical *** general,these findings emphasize the relationship between preload force,SOH and heat generation power,which is helpful for the judgment of optimal preload to improve the efficiency of LIBs.

关键词： lithium ion battery cyclic aging preload force heat generation characteristic

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Programmable synthetic receptors:the next-generation of cell and gene therapies

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Signal Transduction and Targeted Therapy 2024年第2期9卷 376-400页

作者： Fei Teng Tongtong cui Li Zhou qingqin Gao qi Zhou Wei Li University of chinese Academy of Sciences Beijing 101408China State Key Laboratory of Stem cell and Regenerative Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Institute for Stem cell and Regeneration Chinese Academy of SciencesBeijing 100101China Beijing Institute for Stem cell and Regenerative medicine Beijing 100101China

cell and gene therapies hold tremendous promise for treating a range of difficult-to-treat ***,concerns over the safety and efficacy require to be further addressed in order to realize their full *** receptors,a synthetic biology tool that can precisely control the function of therapeutic cells and genetic modules,have been rapidly developed and applied as a powerful *** designed and engineered,they can be applied to finetune the therapeutic activities,i.e.,to regulate production of dosed,bioactive payloads by sensing and processing user-defined signals or *** review provides an overview of diverse synthetic receptor systems being used to reprogram therapeutic cells and their wide applications in biomedical *** a special focus on four synthetic receptor systems at the forefront,including chimeric antigen receptors(cARs)and synthetic Notch(synNotch)receptors,we address the generalized strategies to design,construct and improve synthetic ***,we also highlight the expanding landscape of therapeutic applications of the synthetic receptor systems as well as current challenges in their clinical translation.

关键词： synthetic landscape payload

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