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Activation of the Astrocytic Endothelin System in Response to Hypoxia

中国临床神经科学 2000年第Z1期8卷 97-页

作者： an ding xua kai m.schmidt-ott scbastian tuschick lutz liefeldt susan lyons helmut kettcnmann martin.paul Institute of Clinical Pharmacology and Toxicology Benjamin Franklin Medical CenterBerlinGermany Dept.Neurology Jinan University GuangzhouP.R.of China Institute of Clinical Pharmacology and Toxicology Benjamin Franklin Medical CenterBerlinGermany max-Delbrück Center for moleeular medicine Berlin-BuchGermany

Objcctive:To determine the gene expression patterns of endothelin (ET)system components in cultured astrocytes(AC),and to examine the direct effcct of hypoxia on ET system gene expression in cultured ***:The ET system was considered to be related to the activation of AC. However,how hypoxia affects the ET system in transcriptional levels remains ***:AC was prepared form mouse brain,and cultured 4 *** further incubated under normoxic or hypoxic conditions for 24h. ET peptide levels were determined by *** transcripts of ET system components were measured by Northern Blot RNA hybridization and ***:In normoxic AC,ET-1,ET converting enzyme(ECE)-2,ETA receptor,and ETB receptor mRNAs were detected by Northern blot hybridization with ETB receptor mRNA appearing to be the predominant receptor ***-3and ECE-1 were only detected by RT-PCR,indicating low expression levels of these *** induced a 1.7-fold increase in ET peptide level in culture supernatants as comparcd to controls(p＜0.001).At the same time,a 3-fold increase of ET-1 mRNA(p＜0.001)was determined by Northern blot RNA analysis,indicating a regulation at the transcriptional *** ETA and ETB receptor mRNASweredownregulated to approximately 20% of control levels(p＜0.001), while ECE-2 mRNA remained ***:These results indicate direct effects of hypoxia on astrocytic ET system gene ***,similar changes observed in ischemic conditions in vivo are likely to be at least partially independent from the modified cerebral microenvironment.

关键词： gene expression Northern Blot Northern blot hybridization

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