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A new antimicrobial compound from the leaves of Dodonaea viscosa for infectious diseases

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Bioactive Materials 2020年第3期5卷 602-610页

作者： Basma Mubarak Hamed Al Bimani Mohammad amzad hossain School of Pharmacy College of Pharmacy and NursingUniversity of NizwaP.O.Box 33616NizwaOman

The main aim of this research work was to isolate and characterize the antimicrobial compounds that can be extracted from the leaves of Dodonaea viscosa(***)and to assess their antimicrobial potency by established in-vitro agar diffusion *** methanol extract was liquefied and fractioned by using a separatory funnel and organic solvents having different *** agar diffusion technique was used to assess the antimicrobial potency of extracts and pure compounds against 5 g(+and-)microbial *** potency results showed that all extracts and isolated pure compounds provided significant antimicrobial potency against the applied microbial *** highest activity chloroform extract was analysed by column chromatography(CC)for the isolation of antimicrobial *** structure of the isolated compounds was characterized based on 1D,2D NMR and MS *** conclusion,purest compounds might be useful as a remedy for infectious diseases.

关键词： Dodonaea viscosa Soxhlet Antimicrobial potency Antimicrobial compounds Chromatography NMR MS

In vitro total phenolics,flavonoids contents and antioxidant activity of essential oil,various organic extracts from the leaves of tropical medicinal plant Tetrastigma from Sabah

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Asian Pacific Journal of Tropical Medicine 2011年第9期4卷 717-721页

作者： M amzad hossain Muhammad Dawood Shah Charles Gnanaraj Muhammad Iqbal Biotechnology Research Institute Universili Malaysia Sabah.Licked Bag No.207388999 Kota KinabaluSabahMalaysia

Objective:To detect the in vitro total phenolics,flavonoids contents and antioxidant activity of essential oil,various organic extracts from the leaves of tropical medicinal plant Tetrastigma from ***:The dry powder leaves of Tetrastigma were extracted with different organic solvent such as *** acetate,chloroform,butanol and aqueous *** total phenolic and total flavonoids contents of the essential oil and various organic extracts such as hexane,ethyl acetate,chloroform,butanol and aqueous ethanol were determined by Folin-Ciocalteu method and the assayed antioxidant activity was determined in vitro models such as antioxidant capacity by radical scavenging activity usingα,α-diphenyl-β-picrylhydrazyl (DPPH) ***:The total phenolic contents of the essential oil and different extracts as gallic acid equivalents were found to be highest in methanol extract(386.22 mg/g) followed by ethyl acetate(190.89 mg/g).chloroform(175.89 mg/g).hexane(173.44 mg/g).and Imtanol extract (131.72 mg/g) and the phenolic contents not detected in essential *** antioxidant capacity of the essential oil and different extracts as ascorbic acid standard was in the order of methanol extract>ethyl acetate extract>chloroform>butanol>hexane extract also the antioxidant activity was not detected in essential ***:The findings show that the extent of antioxidant activity of the essential oil and all extracts are in accordance with the amount of phenolics present in that *** of Tetrastigma being rich in phenolics may provide a good source of antioxidant.

关键词： Total phenolics Flavonoids Antioxidant aotivity Essential oil Extracts Tetrastigma

Comparative phytochemical,thin layer chromatographic profiling and antioxidant activity of extracts from some Indian herbal drugs

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Journal of Bioresources and Bioproducts 2022年第2期7卷 46-52页

作者： Md Sohail Akhtar Mohamed Rafiullah Wegdan Aly Shehata amzad hossain Mohammed Ali School of Pharmacy College of Pharmacy and NursingUniversity of NizwaNizwaOman Phytochemistry Research Laboratory Department of Pharmacognosy and PhytochemistryFaculty of PharmacyJamia HamdardNew DelhiIndia Strategic Center for Diabetes Research College of MedicineKing Saud UniversityRiyadhSaudi Arabia

Natural antioxidants play a significant role to prevent cell breaking and regenerate *** plants native to India named dwarf morning-glory(Evolvulus alsinoides),Indian banyan(Ficus bengalensis)and chicory(Chicorium intybus)are used as medicine in India traditionally to treat *** aim of this study is to evaluate and compare the antioxidant activity as well as to compare phytochemical profiles of the aqueous extracts of the selected three *** dried plant powder was extracted with ethanol individually by using the Soxhlet method for eight hours and the solvent was evaporated under reduced ***,the air-dried powder was ex-tracted with water,and the water was *** thin layer chromatography(TLC)profile of the extracts was determined by using TLC densitometer and the antioxidant activity was deter-mined by the conventional 2,2-diphenyl-1-picrylhydrazyl(DPPH)*** TLC profile shows that there are no similarities of retention factor(R f)values among the plant extracts which means all three plants contain different chemical *** antioxidant activities of the aqueous extracts at different concentrations show different antioxidant *** inhibition is dose dependent,as doses are increasing then percentage inhibition is also increasing for all three plant *** the selected plants,the highest activity was found in *** and the lowest was in *** order of the antioxidant activity among the selected plants was ***<***<*** on the TLC profile and antioxidant activities,the highest activity of the selected plant species was evaluated.

关键词： Indian drug Thin layer chromatography(TLC)profile Plant extract Antioxidant activity

Characterization of antioxidant and antibacterial compounds from aerial parts of Haplophyllum tuberculatum

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Journal of Bioresources and Bioproducts 2022年第1期7卷 52-62页

作者： Salma Salim Al-Saeghi Mohammad amzad hossain Salem Said Jaroof Al-Touby School of Pharmacy College of Pharmacy and NursingUniversity of NizwaP.O.Box 33Postal Code 616NizwaOman School of Nursing College of Pharmacy and NursingUniversity of NizwaP.O.Box 33Postal Code 616NizwaOman

The aerial parts of Haplophyllum tuberculatum(***)have traditionally been used by local communities to treat fever,gastrointestinal disorders,worms,malaria and fractures,how-ever,the responsible ingredients of the selected medicinal plant have not been *** the present study,antibacterial and antioxidant ingredients were isolated and characterized from the plant parts of locally grown ***,and their biological activity was *** powder aerial parts were extracted with methanol and the extract was successively frac-tionated with different polarities of *** polarities of extracts(hexane,chloroform,ethyl acetate,butanol,methanol and water)were used to determine their antibacterial and antioxi-dant activity by established in-vitro *** disk diffusion and free radical scavenging 2,2-diphenyl-1-picrylhydrazyl(DPPH)methods were used to evaluate antibacterial and antioxidant activities of the prepared extracts and pure isolated compounds at different concentrations against three strains of Gram-positive and three Gram-negative *** antibacterial activity results showed that all prepared fractions and isolated pure compounds of the plant species gave signif-icant activity against all Gram(+and-)bacterial strains with the range of inhibition 0-20 *** antioxidant activity of the prepared extracts at all concentrations also showed substantial *** on the highest activity,ethyl acetate and chloroform extracts were selected for the separation and isolation of antioxidant and antibacterial compounds by thin layer chromatogra-phy(TLC),column chromatography(CC),and preparative thin layer chromatography(PTLIC).The structure of the isolated antioxidant and antibacterial compounds from the ethyl acetate and chloroform extracts were determined on the basis of nuclear magnetic resonance(NMR)and mass *** isolated pure compounds and active extracts could be used as a medicine for various infectious related di

关键词： H.tuberculatum Soxhlet extraction Antioxidant activity Antibacterial activity Chromatography Pure compound

The ASEAN experience of the purchasing power parity theory

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Financial Innovation 2018年第1期4卷 333-343页

作者： S.M.Woahid Murad Mohammad amzad hossain Department of Economics Noakhali Science and Technology UniversityNoakhali 3814Bangladesh Department of Economics Jahangirnagar UniversitySavarDhaka 1342Bangladesh

We used panel data analysis to evaluate the relative purchasing power parity(PPP)hypothesis of the ten ASEAN member countries between 1973 and *** incorporated the cross-sectionally augmented panel unit root test as proposed by Pesaran(J Appl Econ 22:265-312,2007).For panel cointegration analysis,we employed the four error-correction-based Westerlund(Oxf Bull Econ Stat 69:709-748,2007)panel cointegration *** Westerlund(Oxf Bull Econ Stat 69:709-748,2007)tests are general enough to permit a large degree of heterogeneity,both in the long-run cointegrating relationship and in the short-run dynamics,and dependence within as well as across the cross-sectional *** check the robustness of the results,we further estimated the cointegration test excluding Indonesia and *** findings support our initial ***,all the results overwhelmingly support the relative PPP ***,the monetary authority would be able to implement a self-regulating monetary *** would also be able to control the exchange rates.

关键词： Purchasing power parity Panel unit root test Panel cointegration test ASEAN countries

Isolation and structural elucidation of antifungal compounds from Curcuma amada

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Asian Pacific Journal of Tropical Medicine 2019年第3期12卷 123-129页

作者： Jesmin Akter Kensaku Takara Md.Zahorul Islam Md.amzad hossain Ayako Sano De-Xing Hou The United Graduate School of Agricultural Sciences Kagoshima University Faculty of Agriculture University of the Ryukyus

Objective: To isolate and identify the antifungal compounds from Curcuma amada. Methods: The antifungal activity was measured by the diameter of colonies grown on Petri dish,microscopic observation,and CLSI microdilution methods. The antifungal compounds were isolated through bioactivity guided purification by using silica gel and high-performance liquid chromatography. Structural identification of the antifungal compounds was conducted using 1 H NMR,13 C NMR,and liquid chromatography-tandem mass spectrometry. Results: The purified antifungal compounds were zederone and furanodienone. These two compounds showed dose-dependent antifungal activity against Fusarium solani sensu lato. The concentration required for 50% growth inhibition(IC50) of FSSL ranged from 115 to 129 μM and 82 to 91 μM for zederone and furanodienone,respectively. Conclusion: This study suggested that the isolated compounds from Curcuma amada could be promising natural antifungal agents to control the diseases caused by Fusarium solani sensu lato.

关键词： Curcuma amada Fusarium solani sensu lato Antifungal compounds Zederone Furanodienone Nuclear magnetic resonance

Composition analysis and antimicrobial activity of essential oil from leaves of Laurus nobilis grown in Oman

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Journal of Bioresources and Bioproducts 2022年第4期7卷 328-334页

作者： Siham Saleh Al-Abri Sadri Abdullah Said Salem Said Al Touby Mohammed amzad hossain Jamal Nasser Al-Sabahi School of Pharmacy College of Pharmacy and NursingUniversity of NizwaPB 33PO 616NizwaNizwa 616Oman School of Nursing College of Pharmacy and NursingUniversity of NizwaNizwa 616Oman Central Instrument Laboratory College of Agriculture and Marine SciencesSultan Qaboos UniversityMuscat 123Oman

Traditionally,Laurus nobilis is used for the treatment of earaches and skin rashes as well as *** most common technique to isolate the oil is hydro-distillation and *** investigation presented in this paper was aimed to unravel the antimicrobial activity and chemical composition of essential oil in the leaves of *** grown in *** essential oil was extracted from the leave of the selected plant species by hydro distillation using Clevenger type *** antimicrobial assay was done by disc diffusion methods against three bac-terial strains,Staphylococcus aureus,Pseudomonas aeruginosa and Escherichia coli,and one fungal strain:Candida *** isolated essential oil was analyzed by gas chromatography-mass spectrometry(GC-MS).The percentage yield of the essential oil was 0.064%(w/w).The essen-tial oil was found actively against the applied bacterial and fungal *** highest activity was found against *** and the lowest was against ***,and the order was ***>***>*** essential oil was also active against the fungus *** range of inhibition zones was 6-14 *** results of GC-MS analysis showed(E)-𝛽-caryophyllene to be the major component about 59.62%.Other major components included𝛼-selinene(14.03%),𝛼-humulene(8.65%),𝛽-selinene(4.99%),and𝛼-pinene(4.98%).In addition,several minor com-ponents were found in the essential oil of *** on the experimental results,it showed that the plant species that grown in Oman were a potential source of therapeutic *** con-clusion,the essential oil and the plant could be used as natural and potential therapeutic agents to treat earaches,skin rashes,and rheumatism.

关键词： Laurus nobilis Clevenger apparatus Essential oil Antimicrobial activity Gas chromatography-mass spectrometry(GC-MS)

Phytochemical studies of various polarities leave crude extracts of Omani Datura metel *** evaluation of their antimicrobial potential

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Journal of Coastal Life Medicine 2015年第3期3卷 214-219页

作者： Ali Saleh Hamed Al-Jafari Mohammad amzad hossain School of Pharmacy College of Pharmacy and NursingUniversity of NizwaP.O.Box 33Postal Code 616NizwaSultanate of Oman

Objective:To identify the chemical constituents and evaluate antimicrobial potential of various crude extracts from leaves of Datura metel grown in ***:The leaf samples were collected from the University of Nizwa and extracted with methanol by using Soxhlet *** isolated crude extract was defatted with distilled water and extracted with solvents of different polarities including hexane,chloroform,ethyl acetate and *** compositions of the crude extracts were analyzed by gas chromatography-mass spectrometer and their antimicrobial potential was evaluated by agar disc diffusion method against one Gram positive bacteria Staphylococcus aureus and two Gram negative bacteria Escherichia coli and Pseudomonus ***:The crude extracts were composed of different organic compounds such as alkaloids,hydrocarbons,aromatic hydrocarbons,organic acids,terpenoids,vitamin *** methanol and its fractionated crude extracts showed antimicrobial potential with inhibition zone in the range of 0-13 ***:The selective crude extract from the leaves of Datura metel could be used as natural antibiotics.

关键词： Datura metel Soxhlet extractor Crude extracts Gas chromatography-mass spectrometry analysis Antimicrobial activity

Characterization, prevalence and antibiogram study of Staphylococcus aureus in poultry

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Asian Pacific Journal of Tropical Biomedicine 2017年第3期7卷 253-256页

作者： Yeasmeen Ali Md.Ashraful Islam Nazmul Hasan Muzahid Mohd.Omar Faruk Sikder Md.amzad hossain Lolo Wal Marzan Department of Genetic Engineering & Biotechnology University of Chittagong Graduate School of Biomedical Sciences Texas Tech University Health Sciences Center

Objective: To reveal the presence of methicillin resistant Staphylococcus aureus(S. aureus)(MRSA) in poultry samples and to determine the antibiogram pattern against five ***: Samples from different poultry farm of Chittagong city, Bangladesh were examined for S. aureus by different biochemical tests and confirmed as MRSA by identifying the presence of mec A gene using PCR. Antibiotic resistance pattern in S. aureus was determined by antibiotic disk diffusion ***: In this study, a total of 60 samples(30 from nasal swabs and 30 from cloacal swabs) were used, of which 54 were confirmed as S. aureus by different biochemical tests. Among these, 12 were confirmed as MRSA by detecting mec A gene using *** antibiogram study, both nasal and cloacal samples showed the highest resistance against penicillin-G and the lowest resistance was observed against ***: Based on the present study, it can be said that different antibiotics are used extensively in poultry that leads to MRSA and is alarming for human health.

关键词： Antibiogram Zoonotic transmission Swab mecA gene Opportunistic fungus