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S-nitrosylation of Cdk5 regulates its kinase activity and dendrite growth during neuronal development

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Jacque P k IP amy k y fu Nancy y IP Division of Life Science State Key Laboratory of Molecular Neuroscience and Molecular Neuroscience CenterThe Hong Kong University of Science and TechnologyClear Water Bay

＜正＞Objective Radial migration is critical for the lamination of cerebral cortex during early brain development. Whereas the migration of neurons requires a precise regulation of the organization and dynamics of cytoskeleton including microtubule and actin network,the underlying signaling mechanisms are not well *** the present study,we investigate the functional roles of a kinase Mst3 in radial migration during cortical *** The radial migration in mouse neocortex was investigated by in utero *** cone collapse assay in cultured hippocampal neurons was performed using confocal ***（1） We identified that Mst3, a serine/threonine kinase highly expressed in the developing mouse brain,regulates the neuronal differentiation. （2） Silencing of Mst3 by in utero electroporation resulted in a significant delay on the neuronal migration in the developing mouse neocortex.（2） kinase activity of Mst3 was essential for its functions in neuronal morphogenesis and migration.（3） Mst3 was co-localized with F-actin at the growth cones of cultured hippocampal neurons,and regulated actin *** kinase activity of Mst3 is important for neuronal morphogenesis and radial migration through modulation of actin dynamics.

关键词： mammalian Sterile 20-like kinase 3 Mst3 neuronal migration actin dynamics

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S-nitrosylation of Cdk5 regulates its kinase activity and de...

Methodology in coronary artery bypass surgery quality assessment

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Anthony H.k.Tsang amy k.y.fu Wing-yu fu kenny k.Chung Nancy y.Ip Division of Life Science Molecular Neuroscience Center and State Key Laboratory of Molecular NeuroscienceThe Hong Kong University of Science and TechnologyClear Water Bay

＜正＞Objective Precise regulation of cyclin-dependent kinase 5（Cdk5）,a member of the cyclin-dependent kinase family,is critical for the proper neuronal development and ***5 is activated through its association with the neuron-specific activator p35 or ***,how its kinase activity is regulated in neurons is not well *** the present study,we investigate whether Cdk5 is regulated by the S-nitrosylation,a post-translational modification of protein,and how the S-nitrosylation of Cdk5 modulates its kinase activity and functions during neuronal *** The kinase activity of Cdk5 upon S-nitrosylation was measured using in vitro kinase *** potential physiological role of S-nitrosylation of Cdk5 in neuronal development was studied by overexpressing Cdk5 or its C83A mutant in hippocampal *** change in the neuronal morphology was examined using confocal ***（1）Cdk5 is S-nitrosylated both in vitro and in vivo.（2）S-nitrosylation of Cdk5 inhibits its kinase activity.（3）Inhibition of the S-nitrosylation of Cdk5 enhances dendrite growth in neurons. Conclusion S-nitrosylation of Cdk5 regulates its activity and dendritic development.

关键词： cyclin dependent kinase 5 p35 dendrite nitric oxide S-nitrosylation

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Journal of Geriatric Cardiology 2022年第11期19卷 867-875页

作者： Zhongmin Li Nicole Shirakawa amy Chen fu-Hai Ji Hong Liu Department of Anesthesiology and Pain Medicine University of California Davis HealthSacramentoUSA Department of Anesthesiology First Affiliated Hospital of Soochow UniversitySuzhouChina

Coronary artery bypass graft(CABG)is associated with a high risk of mortality and morbidity;thus,assessment of surgery quality is *** this perspective,we will focus on the structure,process,and outcomes measured as quality assessment.A set of 21 evidence-based structure,process,and outcome measures were selected as National Quality *** these,the Society of Thoracic Surgeons ultimately chose 11 individual quality measures grouped them into four domains used to assess the quality of *** four domains consisted of perioperative medical care,operative care,risk-adjusted operative mortality and postoperative risk-adjusted major *** measures have been useful as quality improvement tools in assessing the quality of CABG surgery.

关键词： bypass surgery mortality

Induction of significant intraocular pressure diurnal fluctuation in rats using a modified technique of microbead occlusion

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International Journal of Ophthalmology(English edition) 2018年第7期11卷 1114-1119页

作者： Lin fu Jimmy Shiu Ming Lai amy Cheuk yin Lo kendrick Co Shih Affiliated Eye Hospital School of Ophthalmology and Optometry Wenzhou Medical University Department of Ophthalmology Li Ka Shing Faculty of Medicine University of Hong Kong

AIM： To investigate the effect of microbead iridocorneal angle occlusion on intraocular pressure（IOP） diurnal fluctuation in rat eyes. METHODS： Male Dark Agouti（DA） rats, 8-10 week old, were each given a single intracameral injection of microbeads, followed by injection of dispersive viscoelastic solution. The right eye served as the experimental eye, while the left eye served as the control. IOP was measured twice daily postoperatively for 3 wk and compared between groups. At the end of 3 wk, the rats were sacrificed and the eyes were harvested for histological analysis and retinal ganglion cell（RGC） counting. RESULTS： After microbead injection, experimental eyes had significantly higher dark time IOP than controls from the second week to the third week [2 nd week： 22.92±1.631 mm Hg（n=5） vs 17.35±0.751 mm Hg（n=5）; 3 rd week： 23.59±1.494 mm Hg vs 17.73±0.592 mm Hg（n=5）], while light time IOP was comparable between groups. The fluctuation levels of IOP in the experimental eyes were 7.21±0.398 mm Hg（n=5）, 9.50±1.017 mm Hg（n=5） and 10.66±0.894 mm Hg（n=5） from the first week to the third week after injection. Comparatively, they were significantly lower in the control eyes, which were 4.69±0.323 mm Hg（n=5）, 2.84±1.122 mm Hg（n=5） and 4.98±0.603 mm Hg（n=5） respectively. However, at the end of 3 wk, the larger fluctuations in IOP in the experimental eyes was not associated with a significant loss of RGCs. CONCLUSION： Microbead occlusion exacerbates diurnal IOP fluctuation in rats. This reported model may serve as a method of investigating the pathological effects of IOP fluctuation. A longer observation period, or repeated injections, may be needed to observe a significant change in RGC density.

关键词： microbead occlusion diurnal intraocular pressure fluctuation glaucoma rat

Comparison of electroretinographic responses between two different age groups of adult Dark Agouti rats

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International Journal of Ophthalmology(English edition) 2015年第5期8卷 898-903页

作者： Lin fu amy Cheuk yin Lo Jimmy Shiu Ming Lai kendrick Co Shih Department of Ophthalmology LKS Faculty of MedicineUniversity of Hong Kong

AIM: To describe and compare the differences in electroretinographic responses between two different age groups of adult Dark Agouti (DA) rats and to better understand the effect of age on retinal histology and function. METHODS: The electroretinographic responses of two different age groups of adult DA rats were compared. Animals were divided into younger adult DA rats 10-12wk (n =8) and older adult DA rats 17-19wk (n=8). full field electroretinography (ERG) was recorded simultaneously from both eyes after dark adaption and light adaption and parameters including the positive scotopic threshold response (pSTR), negative scotopic threshold response (nSTR), scotopic a -wave, b -wave, photopic a -wave, b -wave and photopic negative response (PhNR) were compared between groups. RESULTS: The older adult rats displayed lower stimulation thresholds of the STRs (pSTR and nSTR) and higher amplitudes of pSTR, scotopic a -wave and b wave, photopic b -wave and PhNR amplitudes, with shorter implicit times. Photopic a-wave amplitudes were however higher in the younger adult rats. CONCLUSION: In summary, for the rod system, photoreceptor, bipolar cell and RGC activity was enhanced in the older adult rats. For the cone system, RGC and bipolar cell activity was enhanced, while photoreceptor activity was depressed in the older adult rats. Such age -related selective modification of retinal cell function needs to be considered when conducting ophthalmic research in adult rats.

关键词： electroretinographic responses Dark Agouti rats age

Shear and bulk viscosity of high-temperature gluon plasma

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Chinese Physics C 2018年第6期42卷 59-66页

作者： Le Zhang De-fu Hou Institute of Particle Physics and key Laboratory of Quark and Lepton Physics(MOE) Central China Normal UniversityWuhan 430079China The College of Post and Telecommunication Wuhan Institute of TechnologyWuhan 430070China

We calculate the shear viscosity（η） and bulk viscosity（ζ） to entropy density（s） ratios η/s and ζ/s of a gluon plasma system in kinetic theory, including both the elastic gg←→gg forward scattering and the inelastic soft gluon bremsstrahlung gg←→ggg processes. Due to the suppressed contribution to η and ζ in the gg←→gg forward scattering and the effective g←→gg gluon splitting, Arnold, Moore and yaffe（amy） and Arnold, Dogan and Moore（ADM） have got the leading order computations for η and ζ in high-temperature QCD matter. In this paper, we calculate the correction to η and ζ in the soft gluon bremsstrahlung gg←→ggg process with an analytic *** find that the contribution of the collision term from the gg←→ggg soft gluon bremsstrahlung process is just a small perturbation to the gg←→gg scattering process and that the correction is at～ 5% level. Then, we obtain the bulk viscosity of the gluon plasma for the number-changing process. furthermore, our leading-order result for bulk viscosity is the formula ζ∝（αs^2 T^3）/（lnαs^-1） in high-temperature gluon plasma.

关键词： shear viscosity bulk viscosity entropy density high-temperature gluon plasma soft gluon bremsstrahlung

Imagery perspective among young athletes: Differentiation between external and internal visual imagery

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Journal of Sport and Health Science 2016年第2期5卷 211-218页

作者： Qiu-Hua yu amy S.N.fu Adeline kho Jie Li Xiao-Hua Sun Chetwyn C.H.Chan Applied Cognitive Neuroscience Laboratory Department of Rehabilitation Sciences The Hong Kong Polytechnic University Department of Rehabilitation Sciences The Hong Kong Polytechnic University Metacognition Developmental Centre Department of Science and Education Guangdong Provincial Bureau of Sports Guangdong Institute of Sport

Purpose： This study aimed to investigate the construct of external visual imagery （EVI） vs. internal visual imagery （IV/） by comparing the athletes＇ imagery ability with their levels of skill and types of sports. Methods： Seventy-two young athletes in open （n = 45） or closed （n = 27） sports and with different skill levels completed 2 custom-designed tasks. The EVI task involved the subject generating and visualizing the rotated images of different body parts, whereas the IVI task involved the subject visualizing himself or herself performing specific movements. Results： The significant Skill-Level x Sport Type interactions for the EVI task revealed that participants who specialized in open sports and had higher skill-levels had a higher accuracy rate as compared to the other subgroups. For the IVI task, the differences between the groups were less clear： those with higher skill-levels or open sports had a higher accuracy rate than those with lower skill-levels or closed sports. Conclusion： EVI involves the visualization of others and the environment, and would be relevant to higher skill-level athletes who engage in open sports. IVI, in contrast, tends to be more self-oriented and would be relevant for utilization by higher skill-level athletes regardless of sport type.

关键词： External visual imagery Internal visual imagery Open sports Skills youth

The associations between resilience and socio-demographic factors in parents who care for their children with congenital heart disease

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International Journal of Nursing Sciences 2022年第3期9卷 321-327,I0002页

作者： amy E.Delaney Mei Rosemary fu Melissa L.McTernan Audrey C.Marshall Jessica Lindberg Ravi R.Thiagarajan Zhuzhu Zhou Jeibei Luo Sharon Glazer Boston College William F.Connell School of NursingChestnut HillMAUSA Rutgers University School of NursingeCamdenCamdenNJUSA Boston College Academic Research ServicesChestnut HillMAUSA University of Toronto The Hospital for Sick ChildrenTorontoONCanada The Ethan M.Lindberg Foundation CaledoniaILUSA Harvard Medical School Department of CardiologyBoston Children's HospitalBostonMAUSA Xiamen University School of Economics&the Chow Center XiamenChina Boston College Boston College Thomas P.O'Neill Jr.LibraryChestnut HillMAUSA The University of Baltimore Yale Gordon College of Arts&Sciences BaltimoreMDUSA

Objective To examine the resilience of parents of children with congenital heart disease and to investigate socio-demographic factors that may influence parents’*** This is a web-based survey study using a cross-sectional design.A purposive sampling method was utilized to recruit 515 parents who care for children with congenital heart *** was assessed using the Dispositional Resilience Scale-Ⅱ.Based on expert-interviews,a questionnaire was designed to collect socio-demographic *** statistics,factor analysis,and linear regressions were used to analyze *** A total of 413 parents completed the survey *** mean resilience score was 3.75(SD=0.61;range=1.89–4.89)with higher scores indicating higher *** linear regression models demonstrated that parents who had lower education levels and lower gross household income had lower resilience(Py to cope with stressful events and mitigate stressors associated with having and caring for children with congenital heart *** education levels and lower gross household income are associated with lower *** increase parents’resilience,nursing practice and nurse-led interventions should target screening and providing support for parents at-risk for lower *** lower education level and financial hardship are factors that are difficult to modify through personal efforts,charitable foundations,federal and state governments should consider programs that would provide financial and health literacy support for parents at-risk for lower resilience.

关键词： Child Congenital heart defects Financial stress Households Parents Resilience

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老年痴呆症的分子机制

生命科学 2014年第6期26卷 550-559页

作者：沈阳陈宇傅洁瑜叶玉如香港科技大学深圳研究院广东省脑科学及疾病与药物研究重点实验室深圳518057 香港科技大学分子神经科学国家重点实验室香港科技大学生命科学部和分子神经科学中心

老年痴呆症是一种多发于老年人群的神经退行性疾病,其发病机理十分复杂,目前主要认为与β-淀粉样蛋白的神经毒性和微管结合蛋白tau的异常修饰相关。将从β-淀粉样蛋白在神经突触功能失调、神经元凋亡、炎症反应中的作用,以及tau蛋白异... 详细信息

老年痴呆症是一种多发于老年人群的神经退行性疾病,其发病机理十分复杂,目前主要认为与β-淀粉样蛋白的神经毒性和微管结合蛋白tau的异常修饰相关。将从β-淀粉样蛋白在神经突触功能失调、神经元凋亡、炎症反应中的作用,以及tau蛋白异常修饰的机理与神经损伤等方面阐述老年痴呆症发病的分子机理,并对相关诊断和治疗手段进行讨论。

关键词： β-淀粉样蛋白 tau蛋白突触损伤神经元凋亡炎症反应