检索结果-南通市图书馆

Do introduced honeybees affect seed set and seed quality in a plant adapted for bird pollination?

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Plant Ecology 2017年第4期10卷 721-729页

作者： amy-Marie Gilpin Justin C.Collette Andrew J.Denham Mark K.J.Ooi David J.Ayre Centre for Sustainable Ecosystem Solutions School of Biological SciencesUniversity of WollongongNorthfields AvenueWollongongNSW 2522Australia NSW Department of Premier and Cabinet Office of Environment and HeritagePO Box 1967HurstvilleNSW 2220Australia Centre for Ecosystem Science School of BiologicalEarth and Environmental SciencesUniversity of New South WalesSydneyNSW 2052Australia

Aims Worldwide,evidence suggests that exotic pollinators can disrupt plant mating ***,few studies have determined if pol-lination by the honeybee Apis mellifera(the world’s most widely introduced pollinator)reduces offspring quality when compared with pollination by native *** Australian Proteaceae provides an excellent opportunity to test the impact of honeybees in pol-lination systems that are adapted to birds and non-flying *** We compared the frequency of flower visitation and foraging behav-iour of birds and insects within seven populations of Banksia *** ericifolia is hermaphroditic and has large nectar-rich,orange inflorescences typical of bird and mammal pollinated *** a subset of the study populations,we compared the qual-ity of seed produced via an exclusion treatment(that only allowed invertebrates to access flowers)with an open-pollination treatment(potentially visited by mammals,birds and invertebrates),by meas-uring seed weight,germination rates(t50),percent germination,seedling height after 14 days since the emergence of the cotyledon and time to emergence of the *** Findings Apis mellifera was the only apparent insect pollinator and the most frequent flower visitor,while the open treatment inflorescences were also frequently visited by avian pollinators,primarily hone-yeater *** foraging behaviour of honeybees and honeyeat-ers showed striking differences that potentially affect patterns of pollen *** made significantly greater proportions of within *** plant movements and only 30%(n=48)of honeybees foraged for pollen(nectar foragers carried no pollen)whilst all birds were observed to contact both stigmas and anthers when foraging for *** these fundamental differences in behaviour,there was little effect of treatment on seed set or *** data show that while honeybees appear to alter patterns of pollen transfer within *** popu

关键词： Apis mellifera Banksia ericifolia Proteaceae seedling performance seed vigour

Incidence and consequences of varicella in children treated for cancer in Guatemala

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

世界儿科杂志:英文版 2016年第3期12卷 320-326页

作者： amy E.Caruso Brown Edwin J.Asturias Mario Melgar Federico A.Antillon-Klussmann Pamela Mettler Myron J.Levin Center for Bioethics and Humanities and Department of Pediatrics SUNY Upstate Medical University Center for Global Health and Department of Epidemiology Colorado School of Public HealthAuroraColoradoUSA National Unit of Pediatric Oncology Guatemala CityGuatemala Department of Biostatistics and Informatics Colorado Biostatistics ConsortiumColorado School of Public HealthAuroraColoradoUSA Section of Pediatric Infectious Diseases Department of PediatricsUniversity of Colorado Denver Anschutz Medical CampusAuroraColoradoUSA

Background:Varicella-zoster virus infection is associated with significant morbidity and mortality in immune-compromised children,despite treatment with antiviral *** varicella vaccine programs have significantly decreased this risk in many highincome countries,but in most low-income and middleincome countries,the burden of varicella in children treated for malignancy is poorly defi ***:We retrospectively reviewed records of children at the National Unit of Pediatric Oncology(UNOP)in Guatemala diagnosed with varicella between January 2009 and March 2013 in order to calculate incidence of varicella and evaluate morbidity,mortality,treatment interruption,and ***:Fifty-nine cases of varicella were *** was 23.4 cases per 1000 person-years(p-y).66.1%of cases occurred in children with leukemia(median age 5.2 years;interquantile range 3.4-7 years)and 41.0%of these occurred during maintenance *** of exposure was identified for 14/59(23.7%)*** were hospitalized(71.2%)and given intravenous acyclovir(64.4%).Eight(13.6%)children required critical care,and two(3.4%)died from disseminated varicella with multiorgan *** was delayed or omitted due to varicella in 50%.No signifi cant differences in outcomes based on nutritional and immunologic status were *** minimum average cost of treatment per episode was 598.75 ***:Varicella is a significant problem in children treated for cancer in Guatemala,where effective post-exposure prophylaxis is *** the absence of universal varicella vaccination,strategies to improve recognition of exposure and the future use of novel inactivated vaccines currently under investigation in clinical trials could mitigate this burden.

关键词： cancer developing countries immunocompromised host oncology varicella

CD28 ligation increases macrophage suppression of t-cell proliferation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2012年第4期9卷 341-349页

作者： Daniel Silberman Amanda Bucknum thomas Bartlett Gabriella Composto Megan Kozlowskil Amanda Walker amy Werda Jackelyn Cua Arlene H Sharpe John E Somerville James E Riggs Department of Biology Rider University Lawrenceville NJ 08648 USA Department of Pathology Harvard Medical School Cambridge MA 02115 USA Immunosciences Bristol-Myers Squibb Princeton NJ 08543 USA

When compared to spleen or lymph node cells, resident peritoneal cavity cells respond poorly to t-cell activation in vitro. the greater proportional representation of macrophages in this cell source has been shown to actively suppress the t-cell response. Peritoneal macrophages exhibit an immature phenotype （MHC class IIlo, B7lo） that reduces their efficacy as antigen-presenting cells. Furthermore, these cells readily express inducible nitric oxide synthase （iNOS）, an enzyme that promotes t-cell tolerance by catabolism of the limiting amino acid arginine. Here, we investigate the ability of exogenous t-cell costimulation to recover the peritoneal t-cell response. We show that CD28 ligation failed to recover the peritoneal t-cell response and actually suppressed responses that had been recovered by inhibiting iNOS. As indicated by cytokine ELISpot and neutralizing monoclonal antibody （mAb） treatment, this ＇cosuppression＇ response was due to CD28 ligation increasing the number of interferon （IFN）-y-secreting cells. Our results illustrate that cellular composition and cytokine milieu influence t-cell costimulation biology.

关键词： CD28 costimulation macrophages suppression

Immunoproteome analysis of soluble and membrane proteins of Shigella flexneri 2457t

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2006年第41期12卷 6683-6688页

作者： amy V Jennison Rubhana Raqib Naresh K Verma School of Biochemistry and Molecular Biology Faculty of ScienceThe Australian National UniversityCanberraACT 0200Australia International Centre for Diarrhoeal Disease Research DhakaBangladesh

AIM: to profile the immunogenic proteins of Shigella flexneri (S. flexneri) expressed during human infection using a proteomic approach. MEtHODS: Soluble and membrane protein extractions of S. flexneri 2457t were separated by two-dimensional gel electrophoresis (2-DE). Proteins were transferred to PVDF membrane and immunoblotted with sera from shigellosis patients. Reactive protein spots were matched to Coomassie stained gels run in parallel, cut out and trypsin digested. Matrix-assisted laser desorption/ionization time of flight-mass spectrometry (MALDI-tOF-MS) was used to determine the peptide mass fingerprints, which were searched in the MASCOt database to identify the protein. RESULtS: A total of 8 immunoreactive proteins were successfully identified from the Coomassie stained gels in three repeats. Six of these proteins have not previously been reported as immunogenic in S. flexneri. these proteins could be potential candidates for vaccine or attenuation studies. CONCLUSION: Soluble and membrane proteins of S. flexneri 2457t have been screened by 2-DE and immunoblotting with sera from shigellosis patients. Eight proteins are identified as immunogenic.

关键词：病理治疗临床免疫细菌感染

Reducing agent and exogenous protease additions,individually and in combination, to wheat-and sorghum-based diets interactively influence parameters of nutrient utilisation and digestive dynamics in broiler chickens

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Nutrition 2016年第4期2卷 303-311页

作者： Peter H.Selle Ha H.truong Leon R.McQuade amy F.Moss Sonia Yun Liu Poultry Research Foundation The University of Sydney Poultry CRC University of New England Australian Proteome Analysis Facility Macquarie University

the objective of the study was to investigate the possibility that tandem inclusions of a reducing agent and a protease may advantage chicken-meat production and to ascertain if the established benefits of including sodium metabisulphite in sorghum-based diets extend to wheat-based diets. the study comprised a 2 x 2 x 2 factorial array of treatments in which either nutritionally iso-nitrogenous and isoenergetic wheat-or sorghum-based diets, without and with sodium metabisulphite(2.75 g/kg), without and with protease(1,000 units/kg) were offered to broiler chickens from 7 to 28 days post-hatch. the effects of dietary treatments on growth performance, nutrient utilisation, protein(N) and starch digestibility coefficients and digestive dynamics were determined. A preliminary investigation into the effects of two treatments on concentrations of free amino acids and glucose in the portal circulation was conducted. there was significant feed grain by sodium metabisulphite interactions(P = 0.03 to 0.005) for parameters of nutrient utilisation(AME, ME:GE ratios, N retention, AMEn). For example, sodium metabisulphite inclusions in sorghum-based diets enhanced AME by 0.18 MJ(12.47 versus 12.29 MJ/kg) but depressed AME by 0.43 MJ(11.88 versus 12.31 MJ/kg) in wheat-based diets. there was a linear relationship between starch:protein disappearance rate ratios in the distal ileum with weight gain(r =-0.484; P = 0.0012) indicating that condensed ratios(or absorption of more protein relative to starch) advantaged growth performance. Concentrations of free amino acids in the portal circulation or the post-enteral availability of certain amino acids, including the branched-chain amino acids, methionine, phenylalanine and threonine, were significantly correlated to FCR. For example, threonine concentrations were negatively correlated to FCR(r =-0.773; P = 0.005). Finally, tandem inclusions of sodium metabisulphite and protease in sorghum-based diets may hold merit but it appears that t

关键词： Amino acids Broiler chickens Protease Sodium metabisulphite

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

How I Work

国际经济合作 2006年第12期 1-1页

作者： amy W.Schulman Partner DLA Piper Rudnick Gray Cary

the Enjoyment of Physical Activity Determines Body Mass Index in Young People with Physical Disabilities

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Open Journal of Preventive Medicine 2014年第5期4卷 241-249页

作者： Shirley S. M. Fong amy S. C. Ha tina W. t. Chan Mei K. Au Department of Sports Science and Physical Education The Chinese University of Hong Kong Hong Kong China Physiotherapy Department Hong Kong Red Cross John F. Kennedy Centre Hong Kong China Physiotherapy Department Kowloon Hospital Hong Kong China

Objective: this study aimed to 1) compare the body mass index (BMI) and patterns of out-of-school activity participation in young people with and without physical disabilities, and 2) examine the relationship between BMI and the activity participation patterns among young people with physical disabilities. Methods: thirty-nine young persons with physical disabilities (mean age ± standard deviation: 18.79 ± 1.99 years) and 70 healthy individuals (mean age ± standard deviation: 18.64 ± 0.74 years) participated in the study. the diversity, intensity, companionship, location and enjoyment of participation in activities were evaluated using the Children’s Assessment of Participation and Enjoyment (CAPE) scale. Body height and weight were obtained and BMI was calculated. Results: People with physical disabilities, regardless of their gender, had significantly lower CAPE-derived scores in almost all types of activity than the control participants (p tly higher in the group with physical disabilities than in the control group (p ther showed that the CAPE score for physical activity explained 17.2% of the variance in BMI (p = 0.021). Conclusion: Young people with physical disabilities generally had lower levels of activity participation and a higher BMI than their healthy counterparts. the perception of enjoyment during physical activities was an important determinant of BMI in this group of participants.

关键词： Exercise Obesity Happiness Disabled Persons

Control of nuclear-cytoplasmic shuttling of Ankrd54 by PKCδ

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Biological Chemistry 2017年第3期8卷 163-174页

作者： amy L Samuels Alison Louw Reza Zareie Evan Ingley Cell Signalling Group Harry Perkins Institute of Medical Research and Centre for Medical Researchthe University of Western Australia Proteomics International Laboratories Ltd Proteowa Pty Ltd SABCMurdoch University Cell Signalling Group School of Veterinary and Health SciencesMurdoch University

AIM to identify and characterize the effect of phosphorylation on the subcellular localization of *** HEK293 t cells were treated with calyculin A, staurosporin or phorbol 12-myristate 13-acetate(PMA). Cells were transfected with eG FP-tagged Ankrd54 with or without Lyn tyrosine kinase(wild-type, Y397 F mutant, or Y508 F mutant). the subcellular localization was assessed by immunofluorescence imaging of cells, immunoblotting of subcellular fractionations. the phosphorylation of Ankrd54 was monitored using Phos-tagt M gel retardation. Phosphorylated peptides were analysed by multiplereaction-monitoring(MRM) proteomic *** Activation of PKC kinases using PMA promoted nuclear export of Ankrd54 and correlated with increased Ankrd54 phosphorylation, assayed using Phos-tag tM gel retardation. Co-expression of an active form of the PKCδisoform specifically promoted both phosphorylation and cytoplasmic localization of Ankrd54, while PKCδ, Akt and PKA did not. Alanine mutation of several serine residues in the amino-terminal region of Ankrd54(Ser14, Ser17, Ser18, Ser19) reduced both PMA induced cytoplasmic localization and phosphorylation of Ankrd54. Using MRM proteomic analysis, phosphorylation of the Ser18 residue of Ankrd54 was readily detectable in response to PMA stimulation. PMA stimulation of cells co-expressing Ankrd54 and Lyn tyrosine kinase displayed increased coimmunoprecipitation and enhanced co-localization in the *** We identify phosphorylation by PKCδ as a major regulator of nuclear-cytoplasmic shuttling of Ankrd54, and its interaction with the tyrosine kinase Lyn.

关键词： Ankrd54 PKC Lyn Btk Phosphorylation

Safe and effective sofosbuvir-based therapy in patients with mental health disease on hepatitis C virus treatment

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Hepatology 2016年第31期8卷 1318-1326页

作者： Lydia Shuk Yee tang Jack Masur Zayani Sims amy Nelson Anu Osinusi Anita Kohli Sarah Kattakuzhy Michael Polis Shyam Kottilil Institute of Human Virology Division of Infectious Diseases University of Maryland School of Medicine Critical Care Medicine Department Clinical CenterNational Institutes of Health Gilead Sciences Inc Creighton University School of Medicine St Joseph’s Hospital Laboratory of Immunoregulation National Institute of Allergy and Infectious Diseases National Institutes of Health

AIM to study impact of baseline mental health disease on hepatitis C virus(HCV) treatment; and Beck's Depression Inventory(BDI) changes with sofosbuvir- andinterferon-based *** this is a retrospective cohort study of participants from 5 studies enrolled from single center trials conducted at the Clinical Research Center of the National Institutes of Health, Bethesda, MD, United States. All participants were adults with chronic HCV genotype 1 infection and na?ve to HCV therapy. two of the studies included HCV mono-infected participants only(SPARE, SYNERGY-A), and 3 included human immunodeficiency virus(HIV)/HCV co-infected participants only(ERADICAtE, PFINPK, and ALBIN). Patients were treated for HCV with 3 different regimens: Sofosbuvir and ribavirin in the SPARE trial, ledipasvir and sofosbuvir in SYNERGY-A and ERADICAtE trials, and pegylated interferon(IFN) and ribavirin for 48 wk in the PIFNPK and ALBIN trials. Participants with baseline mental health disease(MHD) were identified(defined as either a DSM Ⅳ diagnosis of major depression, bipolar disorder, schizophrenia, generalized anxiety, and post-traumatic stress disorder or requiring anti-depressants, antipsychotics, mood stabilizers or psychotropics prescribed by a psychiatrist). For our first aim, we compared sustained virologic response(SVR) and adherence(pill counts, study visits, and in 25 patients, blood levels of the sofosbuvir metabolite, GS-331007) within each study. For our second aim, only patients with HIV coinfection were evaluated. BDI scores were obtained pre-treatment, during treatment, and post-treatment among participants treated with sofosbuvir-based therapy, and compared to scores from participants treated with interferon-based therapy. Statistical differences for both aims were analyzed by Fisher's Exact, and t-test with significance defined as a P value less than *** Baseline characteristics did not differ significantly between all participants with and without MHD gro

关键词： Sofosbuvir 指导代理 antivirals 直接行动抗病毒丙肝心理健康疾病消沉干扰素 Beck&rsquo s 消沉库存