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Investigation on the Epidemiology and Pathogenesis Characteristics of Staphylococcus pseudintermedius-Associated Veterinary Hospital Infections

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Advances in Microbiology 2023年第6期13卷 267-283页

作者： Shelby Matsuoka Jenna Beilby Joelle Jacob Sophia Selliken Bailey Keefe amy leestemaker-palmer Luiz E. Bermudez Department of Biomedical Sciences Carlson College of Veterinary Medicine Oregon State University Corvallis OR USA Diagnostic Laboratory Carlson College of Veterinary Medicine Oregon State University Corvallis OR USA Department of Microbiology College of Science Oregon State University Corvallis OR USA

A retrospective analysis of the diagnostic laboratory submissions from 2016 to 2020 was performed to assess the antibiotic resistance of Staphylococcus pseudintermedius and determine locations in the hospital which might be acting as environmental source(s). Previous studies have identified a significant increase in infections with S. pseudintermedius. Samples were taken from the hospital environment by swabbing areas in the intensive care unit and anesthesia preparation room and bacterial species identified. Isolates obtained from patients were then examined regarding the ability to form biofilm, an important phenotype on hospital-related infection. In addition, veterinary hospital associated strains of S. pseudintermedius were used to determine the bactericidal effect of the used disinfectant, applying the hospital current protocol, by comparing the efficacy against S. pseudintermediius and a strain of Staphylococcus aureus from a dog. The isolates identified were resistant to commonly used antibiotics such as enrofloxacin and cephalosporins, and 45% percent of those were methicillin resistant. The environmental survey in the hospital identified S. pseudintermedius in the pre-anesthesia area, although the isolate was killed by the current used disinfection protocol. A few disease associated bacteria were evaluated for biofilm formation in comparison to a dog isolate of Staphylococcus aureus, demonstrating strong ability to form biofilms.

关键词： Staphylococcus pseudintermedius Veterinary Hospital Infection Epidemiology Biofilm Antibiotic Susceptibility Environment

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Investigation on the Epidemiology and Pathogenesis Characteristics of Staphylococcus pseudintermedius -Associated Veterinary Hospital Infections

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Advances in Microbiology（微生物学） 2023年第6期

作者： Shelby Matsuoka Jenna Beilby Joelle Jacob Sophia Selliken Bailey Keefe amy leestemaker-palmer Luiz E. Bermudez

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Cytoprotective effect of amniotic membrane extracts on human corneal epithelial cells exposed to benzalkonium chloride in vitro

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Annals of Eye Science 2020年第2期5卷 1-11页

作者： Jiayan Liu Huyong Zou Minzhi Zeng amy Michelle Huang Yan Chen Elaine Han Xiangyin Sha Zhiping Liu Ophthalmic Center the Second Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510260China Department of Ophthalmology the Sixth Affiliated Hospital of Guangzhou Medical UniversityQingyuan 511518China Department of Ophthalmology Bascom Palmer Eye InstituteUniversity of Miami Miller School of MedicineMiamiFLUSA

Background:The goal was to explore the protective effect and potential mechanism of amniotic membrane extracts(AME)on the ocular surface exposed to benzalkonium chloride(BAC).Methods:The human corneal epithelial cell(HCEC)line SD-HCEC1s was cultured in 5 groups:normal control(NC),NC+AME,BAC,BAC+NC,and BAC+*** viability analysis,flow cytometry analysis,real-time polymerase chain reaction(PCR),and western blot were employed to measure changes in cell *** metalloproteinases(MMPs)and inflammatory cytokines were assayed by enzyme-linked immunosorbent assay(ELISA)and activity ***:Real-time PCR and western blot analysis demonstrated that the expressional level of caspase-8 was increased while the levels of Muc1,Muc4,and Muc16 were decreased after treatment with 0.02%BAC for 1 *** the SD-HCEC1s were withdrawn from the BAC and switched to media containing 10%AME for 2 days,the expression level of capsase-8 was decreased while the levels of Muc1,Muc4,and Muc16 were ***-time PCR and ELISA demonstrated that the mRNA and protein levels of MMP-1,MMP-3,MMP-13,CXCL1,interleukin(IL)-1β,IL-6,and tumor necrosis factor-alpha(TNF-α)were significantly increased after treatment with 0.02%BAC,whereas those of MMP-8 were *** the 0.02%BAC was withdrawn and the SD-HCEC1s were cultured in 10%AME,the mRNA and protein levels of MMP-1,MMP-3,MMP-13,CXCL1,IL-1β,IL-6,and TNF-αwere decreased,while those of MMP-8 were ***-8 activity assays confirmed that IL-1βand TNF-αdownregulated the protein levels of ***:AME protects SD-HCEC1s when stressed in BAC via upregulation of MMP-8 and downregulation of IL-1βand TNF-α.AME may have the potential functions to be employed as a topical adjunctive therapy in eyes chronically exposed to BAC.

关键词： Amniotic membrane(AM) human corneal epithelial cell(HCEC) benzalkonium chloride(BAC) dry eye

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