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Anti-Osteoporosis and Anti-Osteoarthritis Activity of Fresh Water Snail <i>(Viviparous bengalensis)</i>Flesh Extract in Experimental Animal Model

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Open Journal of Rheumatology and Autoimmune Diseases 2013年第1期3卷 10-17页

作者： amrita sarkar Poulami Datta Aparna Gomes Subir Chandra Das Gupta Antony Gomes Department of Zoology Maulana Azad College Kolkata India Indian Institute of Chemical Biology Kolkata India Laboratory of Toxinology & Experimental Pharmacodynamics Department of Physiology University of Calcutta Kolkata India

Aims: Aim was to evaluate the anti-osteoporotic and anti-osteoarthritic activity of fresh water snail (Viviparous bengalensis) (VB) flesh extract (VBE) in experimental model. Settings and Design: Experimental osteoporosis (OSP) was developed in female Wistar rats by bilateral overectomy and Osteoarthritis (OA) was developed in male Wistar rats by bacterial collagenase injection. Methods and Material: VB was collected locally and authenticated, then homogenized in 3500 rpm × 15 mins and supernatant was collected. Rats were divided into-Group-1: Sham control, Group-2: OSP/OA control, Group-3: Standard (vitD3 200 mg·kg -1;p.o., calcium i.p. 1500 mg·kg -1?× 15 days in OSP and indomethacin 0.25 mg·kg-1, p.o. × 5 alternative days in OA), Group-4: VBE treated (1 gm·kg-1;p.o. × 15 days), Group-5: VBE treated (2 mg·kg-1;p.o. × 15 days). Anti-osteoporotic and anti-osteoarthritic activity of VBE was examined through physical, urinary and serum parameters. Statistical use: Data were expressed in terms of mean ± SEM (n = 6). ANOVA was performed, p Key findings: It was observed that the body weight, ankle/ knee diameters, urinary markers hydroxyproline/glucosamine/calcium/phosphate/creatinine, serum ACP/ALP/TRAP, calcium/creatinine, cytokines (TNF-α/IL-1β/CINC-1) levels were changed significantly and restored after VBE treatment. Significance: Fresh water snail flesh extract possess anti-osteoporosis and anti-osteoarthritic activity in experimental animal models.

关键词： Water Snail Viviparous bengalensis Traditional Medicine Osteoporosis Osteoarthritis

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Recombinase Polymerase Amplification Based Rapid Detection of Aroma Gene in Rice

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Rice science 2023年第2期30卷 96-99,I0010-I0013页

作者： amrita BANERJEE S.BHARTI J.KUMAR P.SAR PRIYAMEDHA N.P.MANDAL S.sarkar Somnath ROY Central Rainfed Upland Rice Research Station Indian Council of Agricultural Research(ICAR)-National Rice Research InstituteHazaribagJharkhand 825301India Department of Biotechnology Vinoba Bhave UniversityHazaribagJharkhand 825301India Crop Improvement Division ICAR-National Rice Research InstituteCuttackOdisha 753006India

Recombinase polymerase amplification(RPA) has been emerged as an alternative to PCR due to its high specificity and sensitivity in diagnostics of animal and plant pathogens. In this study, an RPA protocol was develope... 详细信息

关键词： specificity Rec emerged

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Pharmacological studies on Buchanania lanzan Spreng.-A focus on wound healing with particular reference to anti-biofilm properties

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Asian Pacific Journal of Tropical Biomedicine 2013年第12期3卷 967-974页

作者： Ashok Pattnaik Ratul sarkar amrita Sharma Kirendra Kumar Yadav Alekh Kumar Paramita Roy Avijit Mazumder Sanmoy Karmakar Tuhinadri Sen Department of Pharmaceutical Sciences Birla Institute of Technology Division of Pharmacology Department of Pharmaceutical TechnologyJadavpur University School of Natural Product Studies Department of Pharmaceutical TechnologyJadavpur University Department of Pharmaceutical Technology Noida Institute of Engineering and Technology

Objective:To evaluate the wound healing activity of the methanolic root extract of Buchanania lanzan Spreng.(***),with a focus on antimicrobial and anti-biofilm ***:The extract was evaluated for its wound healing properties(excision and incision models)as evident from the analysis of tensile strength and wound *** extract was also screened for antibacterial properties against different Cram positive and Gram negative bacterial *** was also studied for its effect on biofilm formation and disruption of preformed *** synergistic effect of *** was determined in combination with ***:Topical application of ***(10%w/w ointment)significantly increased(40.84%)the tensile strength in the incision wound *** also showed significant wound healing activity in excision model and such significant activity was observed from the 9th *** Soframycin displayed significant wound healing activity from the 6th *** was found that root extracts of *** revealed significant inhibition against all tested *** displayed antimicrobial activity against Cram positive(MIC 0.625 mg/mL)and Cram negative(MIC0.625-1.25 mg/mL).*** was able to reduce biofilm formation and also caused disruption of preformed biofilms in a manner similar to ***,gentamicin was found to be ineffective against biofilms formed by Gram negative *** to the fractional inhibitory concentration index,*** displayed synergistic activity when it was combined with ***:From this study it may be concluded that the root extract of *** revealed significant wound healing potential,which was supported and well correlated with pronounced antibacterial activity of the tested plant parts.

关键词： Buchanania lanzan Root and leaf extracts Wound healing Incision Excision Antibacterial Antibiofilm

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