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Killing of Staphylococcus aureus by allylpyrocatechol is potentiated by induction of intracellular oxidative stress and inhibition of catalase activity

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Journal of Integrative Medicine 2016年第6期14卷 456-464页

作者： Roslinah Mohamad Hussain Noor Faradilla Abdullah zulkhairi amom Department of Medical Laboratory Technology Faculty of Health SciencesUniversiti Teknologi MARA Department of Anatomy and Basic Sciences Faculty of Health SciencesUniversiti Teknologi MARA

OBJECTIVE： This study investigated the effects of allylpyrocatechol （APC）, the major component in ethanolic extract of Piper betle, on key oxidative stress resistance enzymes important for the survival of Staphylococcus aureus, a major pathogen in the human host. METHODS： Effects of APC on expressions of genes encoding catalase （katA）, superoxide dismutases （SODs）, including soda and sodM, and alkyl hydroperoxide reductase （ahpC） in S. aureus were quantitated by RT-qPCR in reference to gyrA and 16S rRNA. Corresponding activities of the enzymes were also investigated. The Livak analysis was performed for verification of gene-fold expression data. Effects of APC on intracellular and extracellular reactive oxygen species （ROS） levels were determined using the nitroblue tetrazolium （NBT） reduction assay. RESULTS： APC-treated S. aureus cells had higher sodA and sodM transcripts at 1.5-fold and 0.7-fold expressions respectively with corresponding increase in total SOD activity of 12.24 U/mL compared to untreated cells, 10.85 U/mL （P〈0.05）. Expression of ahpC was highest in APC-treated cells with 5.5-fold increased expression compared to untreated cells （P〈0.05）. Correspondingly, ahpC activity was higher in APC-treated cells at 0.672 （A310nm） compared to untreated cells which was 0.394 （A310nm）. In contrast, katA expression was 1.48-fold and 0.33-fold lower respectively relative to gyrA and 16S rRNA. Further, APC-treated cells showed decreased catalase activity of 1.8 × 10^-4 （U/L or μmol/（min.L）） compared to untreated cells, which was 4.8 × 10^-4 U/L （P〈0.05）. Absorbance readings （A575nm） for the NBT reduction assay were 0.709 and 0.695 respectively for untreated and treated cells, which indicated the presence of ROS. APC-treated S. aureus cells had lower ROS levels both extracellularly and intracellularly, but larger amounts remained intracellularly compared to extracellular levels with absorbances of 0.457 and 0.137 respectively （P〈0.05）. CONCLUSION： APC induced express

关键词： allylpyrocatechol catalase superoxide dismutase alkyl hydroperoxide reductase reactiveoxygen species Staphylococcus aureus Piper beUe

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Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第5期11卷 357-365页

作者： Abdul Hamid HAFIZAH Zakaria ZAITON amom zulkhairi Adenan MOHD ILHAM Megat Mohd Nordin NOR ANITA Abdullah Mahdy ZALEHA Department of Physiology Universiti Kebangsaan Malaysia Medical CentreJalan Raja Muda Aziz50300 Kuala LumpurMalaysia Department of Human Anatomy Division of PhysiologyFaculty of Medicine and Health SciencesUniversiti Putra Malaysia Forest Research Institute of Malaysia Department of Obstetrics and Gynecology Universiti Kebangsaan Malaysia Medical Centre

Endothelial cell death due to increased reactive oxygen species(ROS) may contribute to the initial endothelial injury,which promotes atherosclerotic lesion *** sarmentosum(PS),a natural product,has been shown to have an antioxidant property,which is hypothesized to inhibit production of ROS and prevent cell ***,the present study was designed to determine the effects of PS on the hydrogen peroxide(H2O2)-induced oxidative cell damage in cultured human umbilical vein endothelial cells(HUVECs).In this experiment,HUVECs were obtained by collagenase perfusion of the large vein in the umbilical cord and cultured in medium M200 supplemented with low serum growth supplementation(LSGS).HUVECs were treated with various concentrations of H2O2(0-1000 μmol/L) and it was observed that 180 μmol/L H2O2 reduced cell viability by 50% as denoted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) *** the above concentration as the positive control,the H2O2-induced HUVECs were concomitantly treated with various concentrations(100,150,250 and 300 μg/ml) of three different extracts(aqueous,methanol and hexane) of ***(MDA),superoxide dismutase(SOD),catalase(CAT) and glutathione peroxidase(GPX) levels showed a significant increase(P<0.05) in HUVECs compared to the negative ***,PS extracts showed a protective effect on HUVECs from H2O2-induced cell apoptosis with a significant reduction in MDA,SOD,CAT and GPX levels(P<0.05).Furthermore,PS had exhibited ferric reducing antioxidant power with its high phenolic ***,it was concluded that PS plays a beneficial role in reducing oxidative stress in H2O2-induced HUVECs.

关键词： Piper sarmentosum Human umbilical vein endothelial cells(HUVECs) Malondialdehyde Oxidative stress Antioxidant enzymes

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Low Doses Of Diclofenac Induces Hepatocellular Changes In Rat Treated In Vivo

Low Doses Of Diclofenac Induces Hepatocellular Changes In Ra...

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第十五届国际药理学大会

作者： Fakurazi Sharidal Ithnin Hairuszah2 amom zulkhairi Baharuldin Taufik Hidayat Moorthy Mohanambal Moorthy Win Maw L Department of Human Anatomy Faculty of Medicine and Health Sciences Universiti Putra Malaysia 43400 UPM SERDANG Selangor Daral Ehsan MALAYSIA Department of Clinical Science Laboratory Faculty of Medicine and Health Sciences Universiti Putra Malaysia 43400 UPM SERDANG Selangor Darul Ehsan MALAYSIA Department of Human Anatomy Faculty of Medicine and Health Sciences Universiti Putra Malaysia 43400 UPM SERDANG Selangor Daral Ehsan MALAYSIA Department of Human Anatomy Faculty of Medicine and Health Sciences Universiti Putra Malaysia 43400 UPM SERDANG Selangor Daral Ehsan MALAYSIA Department of Human Anatomy Faculty of Medicine and Health Sciences Universiti Putra Malaysia 43400 UPM SERDANG Selangor Daral Ehsan MALAYSIA Department of Human Anatomy Faculty of Medicine and Health Sciences Universiti Putra Malaysia 43400 UPM SERDANG Selangor Daral Ehsan MALAYSIA

＜正＞ Diclofenac is reported to have cytotoxic effects and induces apoptosis in various cell lines. This study is conducted to investigate the mechanism of apoptosis in liver of rat treated with diclofenac. Rats were dosed with 3. 5 and 10mg/kg diclofenac in saline for 15 days. Livers were then removed, weighed and processed for histopathological analysis. 10 mg/kg diclofenac after 15 days treatment found to trigger the accumulation of inflammatory cells such as lymphocytes and nuetrophils. This was observed mainly in the centrilobular region. Apoptotic cells were observed following TUNEL assay and were also found to be the hepatocytes at the centrilobular area. This evidence was supported by the changes seen in the mitochondria membrane observed using TEM.

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