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Emerging technology of in situ cell free expression protein microarrays

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Protein & Cell 2012年第2期3卷 240-240页

作者： amita nand Anju Gautam Javier Batista Pérez Alejandro Merino Jinsong Zhu National Center for Nanoscience and Technology Chinese Academy of SciencesBeijing 100190China Institute of Microbiology Chinese Academy of SciencesBeijing 100101China

Erratum to:Protein Cell 2012,3(2):84-88 DOI 10.1007/s13238-012-2012-y Due to a typesetting error,(~3-7×10^(13)mol/L)in the fifth line of the“TUS-TER microarray”subsection on the right column of page 85 should be(~... 详细信息

关键词： microarray free fifth

Emerging technology of in situ cell free expression protein microarrays

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Protein & Cell 2012年第02期 84-88页

作者： amita nand Anju Gautam Javier Batista Pérez Alejandro Merino Jinsong Zhu National Center for Nanoscience and Technology Chinese Academy of Sciences Institute of Microbiology Chinese Academy of Sciences

Recently, in situ protein microarrays have been developed for large scale analysis and high throughput studies of proteins. In situ protein microarrays produce proteins directly on the solid surface from pre-arrayed DNA or RNA. The advances in in situ protein microarrays are exemplified by the ease of c DNA cloning and cell free protein expression. These technologies can evaluate, validate and monitor protein in a cost effective manner and address the issue of a high quality protein supply to use in the array.

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Oxidative Stressand Antioxidant Responses in Young Leaves of Mulberry Plants Grown Under Nitrogen, Phosphorus or Potassium Deficiency

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Journal of Integrative Plant Biology 2007年第3期49卷 313-322页

作者： Rajesh Kumar Tewari Praveen Kumar Parma nand Sharma Department of Botany University of LucknowLucknow226007India

The aim of this study was to associate the generation of reactive oxygen species （ROS） with Induced antloxidant responses and disturbed cellular redox environment in the nitrogen-（N）, phosphorus-（P）, or potassium-（K） deftcient mulberry （Morus alba L. var. Kanva-2） plants. The indicators of oxidative stress and cellular redox environment and antioxldant defense-related parameters were analyzed. Oeficlency of N, P or K suppressed growth, accelerated senescence, and decreased concentrations of chloroplastic pigments and glutathione. Lipid peroxidation and activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase were also increased in these N, P, or K deprived plants. Concentration of hydrogen peroxide Increased in plants deficient in N or P. Oeficlency of N or P particularly altered the cellular redox environment as indicated by changes in the redox couples, namely ascorbic acid/total ascorbate decreased in P-, glutathione sulfydryl/total glutathione decreased in N-, and Increased in P-deficient plants. Activity staining of native gels for superoxide dismutase revealed Increased activity as Indicated by Increased intensity of bands, and induction of few new isoforms in P- and K-deficient plants. Oifferences in the patterns of superoxide dismutase isoforms and redox status （ascorbic acid/total ascorbate and glutathlone sulfydryl/total glutathione） Indicate that N-, P-, or K-deficiency altered antioxidant responses to varying extents in mulberry plants.

关键词： antioxidants potassium-deficiency lipid peroxidation Morus alba mulberry nitrogen-deficiency oxidative stress phosphorus-deficiency reactive oxygen species

Importance of retrieving noun phrases and named entities from digital library content

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Journal of Zhejiang University-Science C(Computers and Electronics) 2010年第11期11卷 844-849页

作者： Ratna SANYAL Kushal KESHRI Vidya nand Indian Institute of Information Technology

We present a novel approach for extracting noun phrases in general and named entities in particular from a digital repository of text *** problem of coreference resolution has been divided into two subproblems:pronoun resolution and non-pronominal resolution.A rule based-technique was used for pronoun resolution while a learning approach for nonpronominal *** named entity resolution,disambiguation arises mainly due to polysemy and *** proposed approach fixes both problems with the help of WordNet and the Word Sense Disambiguation *** proposed approach,to our knowledge,outperforms several baseline techniques with a higher balanced F-measure,which is harmonic mean of recall and *** improvements in the system performance are due to the filtering of antecedents for the anaphor based on several linguistic disagreements,use of a hybrid approach,and increment in the feature vector to include more linguistic details in the learning technique.

关键词： Coreference resolution Hybrid approach Filtering Rule based and J48 algorithm

Exploring the potential of artificial intelligence techniques in prediction of the removal efficiency of vortex tube silt ejector

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International Journal of Sediment Research 2023年第4期38卷 615-627页

作者： Sanjeev Kumar Chandra Shekhar Prasad Ojha nand Kumar Tiwari Subodh Ranjan Department of Civil Engineering Indian Institute of Technology Department of Civil Engineering National Institute of Technology

A vortex tube silt ejector is a curative hydraulic structure used to remove sediment deposits from canals and is recognized as one of the most efficient substitutes for physically removing canal sediment. The spatially varied flow in the channel and the rotational flow behavior in the tube make the silt removal process complex. It is even harder to accurately predict the silt removal efficiency by traditional models accurately. However, artificial intelligence(AI) and machine learning approaches have emerged as robust substitutes for studying complex processes. Therefore, this research makes use of AI approaches; support vector machine(SVM), random forest(RF), random tree(RT), and multivariate adaptive regression spline(MARS) to compute the vortex tube silt ejection efficiency using the laboratory data sets. The outcomes of the artificial intelligence(AI)-based techniques also were compared with traditional models. It was found that the RT model(root mean square error, RMSE = 2.165, Nash Sutcliffe efficiency, NSE = 0.98) outperforms the other applied approaches which had relatively more significant result errors. The sensitivity analysis of the process depicts the extraction ratio as the key parameter in the computation of vortex tube silt ejector removal efficiency. The findings of the AI-based approaches discussed in the current study might be helpful for hydraulic engineers as well as researchers in the assessment of the removal efficiency of vortex tube silt ejectors.

关键词： Vortex tube silt ejector Support vector machine Random forest Random tree Multivariate adaptive regression spline

Phosphorus Starvation Tolerance in Rice Through Combined Physiological,Biochemical,and Proteome Analyses

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Rice science 2023年第6期30卷 613-631页

作者： Prathap V Suresh KUMAR nand Lal MEENA Chirag MAHESHWARI Monika DALAL Aruna TYAGI Division of Biochemistry Indian Council of Agricultural Research-Indian Agricultural Research InstituteNew Delhi 110012India Division of Germplasm Evaluation National Bureau of Plant Genetic ResourcesNew Delhi 110012India National Institute for Plant Biotechnology New Delhi 110012India

Phosphorus(P) deficiency limits the growth,development,and productivity of *** better understand the underlying mechanisms in P-deficiency tolerance and the role of Pup1 QTL in enhancing P use efficiency(PUE) for the development of P-efficient rice cultivars,a pair of contrasting rice genotypes(Pusa-44 and NIL-23) was applied to investigate the morpho-physio-biochemical and proteomic variation under P-starvation *** rice genotypes were grown hydroponically in a PusaRich medium with adequate P(16 mg/kg,+P) or without P(0 mg/kg,-P) for 30 d.P-starvation manifested a significant reductions in root and shoot biomass,shoot length,leaf area,total chlorophyll,and P,nitrogen and starch contents,as well as protein kinase *** stress increased root-to-shoot biomass ratio,root length,sucrose content,and acid phosphatase activity,particularly in the P-tolerant genotype(NIL-23).Comparative proteome analysis revealed several P metabolism-associated proteins(including OsCDPKs,OsMAPKs,OsCPKs,OsLecRK2,and OsSAPks) to be expressed in the shoot of NIL-23,indicating that multiple protein kinases were involved in P-starvation/deficiency ***,the up-regulated expression of OsrbcL,OsABCG32,OsSUS5,OsPoll-like B,and ClpC2 proteins in the shoot,and OsACA9,OsACA8,OsSPS2F,OsPP2C15,and OsBiP3 in the root of NIL-23,indicated their role in P-starvation stress control through the Pup1 QTL. Thus,our findings indicated that-P stress-responsive proteins,in conjunction with morpho-physio-biochemical modulations,improved PUE and made NIL-23 a P-deficiency tolerant genotype due to the introgression of the Pup1 QTL in the Pusa-44 background.

关键词： phosphorus deficiency Pup1 QTL protein kinase acid phosphatase proteome analysis rice

Unsupervised machine learning-based clustering identifies unique molecular signatures of colorectal cancer with distinct clinical outcomes

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Genes & Diseases 2023年第6期10卷 2270-2273页

作者： Manish Pratap Singh Sandhya Rai Sarvesh K.Gupta nand K.Singh Sameer Srivastava Motilal Nehru National Institute of Technology Allahabad Prayagraj 211004India Deen Dayal Upadhyay Gorakhpur University Gorakhpur 273001India

Colorectal cancer(CRC)is known to harbor considerable heterogeneity.1 Consequently,it could be hypothesized that similar-appearing tumors might exhibit substantial genetic differences while diverse-appearing tumors may have a similar genetic landscape.2 Due to these differences at the molecular level,they behave or respond differently to therapies as *** progression is a multistep process and involves the accumulation of substantial genetic and epigenetic events in a stage-dependent manner.

关键词： cancer colorectal signature

Molecular subtypes of colorectal cancer: An emerging therapeutic opportunity for personalized medicine

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Genes & Diseases 2021年第2期8卷 133-145页

作者： Manish Pratap Singh Sandhya Rai Ashutosh Pandey nand K.Singh Sameer Srivastava Department of Biotechnology Motilal Nehru National Institute of TechnologyAllahabadUP211004India

Molecular subtypes-based therapies offer new potential framework for desired and precise outcome in clinical *** treatment strategies in colorectal cancer are largely‘one drug fit all’model for patients that display same pathological ***,CRC is a very heterogenous set ofmalignancy that does not support for above *** subtype displays different pathological and genetic *** on these features,therapeutic stratification for individual patients may be designed,which may ultimately lead to improved therapeutic *** this comprehensive review,we have attempted to briefly outline major CRC pathways.A detailed overview of molecular subtypes and their clinical significance has been *** and futuremethods,governing CRC subtyping in the era of personalized therapywith a special emphasis on CMS subtypes of CRC has been ***,discovery and validation of new CRC patient stratification methods,screening for novel therapeutic targets,and enhanced diagnosis of CRC may improve the treatment outcome.

关键词： Biomarkers CMS subtypes Immunotherapy Molecular screening Personalized medicine

Hexagonal yttrium manganite:A review on synthesis methods,physical properties and applications

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Journal of Rare Earths 2023年第1期41卷 19-31页

作者： Bhagyashree Munisha Bindhybasinee Mishra Jyotirmayee nanda Centre for Nanoscience and Nanotechnology ITERS‘O’A(Deemed to be)UniversityKhandagiriBhubaneswar751030OdishaIndia Department of Physics ITERS‘O’A(Deemed to be)UniversityKhandagiriBhubaneswar751030OdishaIndia

Hexagonal rare-earth manganites have attracted considerable interest due to their complex multiferroic properties,and they possess a spontaneous electric polarization and may be utilized in various technological applications ranging from solar cells to non-volatile memories and light *** narrow bandgap of hexagonal rare-earth manganite-based compounds can perform remarkable optical anisotropy properties,which make these very efficient photovoltaic materials in parallel with the widely used TiO_(2)-based *** enlargement of the traditional field of hexagonal rare-earth manganite photocatalysis also arose in the last *** article imparts an extensive review of the rational synthesis,optical properties,structural characterization,and applications of hexagonal rare-earth *** properties of hexagonal rare-earth perovskite manganite are also emphasized,and a range of unique applications in photocatalysis and photovoltaics are ***,we wind up this review with some linear perspectives and future research in this area.

关键词： Yttrium manganite Multiferroic Optical analysis Photocatalysis Photovoltaics Rare earths