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Anti-cancer effects of sitagliptin,vildagliptin,and exendin-4 on triple-negative breast cancer cells via mitochondrial modulation

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BIOCELL 2022年第12期46卷 2645-2657页

作者： POOJA JAISWAL VERSHA TRIPATHI ANSHUL ASSAIYA DHARMENDRA KASHYAP RAHUL DUBEY ANAMIKA SINGH JANESH KUMAR HEM CHANDRA JHA RAJESH SHARMA amit KUMAR dixit HAMENDRA SINGH PARMAR School of Biotechnology Devi Ahilya UniversityIndore452001India Laboratory of Membrane Protein Biology National Centre for Cell SciencePune411007India Department of Biosciences and Biomedical Engineering Indian Institute of TechnologyIndore452020India Department of Biotechnology IPS AcademyIndore452012India School of Pharmacy Devi Ahilya UniversityIndore452001India Central Council for Research in Ayurvedic Sciences Kolkata263645India

Triple-negative breast cancer(TNBC)cell line MDA-MB-231 is known for Warburg metabolism and defects in *** the other hand,dipeptidyl peptidase-IV(DPP-IV)inhibitors such as sitagliptin and vildagliptin and GLP-1 agonist exendin-4 are known to improve mitochondrial functions as well as biogenesis,but no study has evaluated the influence of these drugs on mitochondrial biogenesis on metastatic breast cancer cell *** have recently reported anticancer effects of 5-aminoimidazole-4-carboxamide riboside on MDA-MB-231 cells via activation of AMP-dependent kinase(AMPK),which activates the downstream transcription factors PGC-1α,PGC-1β,or FOXO1 for mitochondrial biogenesis;above-mentioned incretin-based therapies are also known to activate *** study evaluated the effects of sitagliptin,vildagliptin,and exendin-4 on MDA-MB-231 cells and the underlying changes in mitochondrial biogenesis,were *** with sitagliptin(100μM),vildagliptin(100μM),and exendin-4(10 nM)for 72 h to MDA-MB-231 cells led to a decrease in viability indicated by MTT assay,cell migration by scratch,and transwell migration assays,accompanied with marginal reduction in cell numbers along with the apoptotic appearance,the rate of apoptosis,and decreased lactate content in conditioned *** changes in the cancer phenotype were accompanied by an increase in the mitochondrial DNA to nuclear DNA ratio,increased MitoTracker green and red staining,and increased expression of transcription factors PGC-1α,NRF-1,NRF-2,TFAM,and ***-treatment of cells with these incretin-based drugs followed by 48 h treatment with 1μM doxorubicin increased doxorubicin sensitivity as observed by a decrease in viability by MTT ***,sitagliptin,vildagliptin,and exendin-4 exert their beneficial effects on TNBC cells via an increase in mitochondrial biogenesis that helps to switch Warburg metabolism into anti-Warburg *** response was in the order of:sitagliptin>vildagliptin>ex

关键词： Sitagliptin Vildagliptin Exendin-4 Apoptosis Migration Viability Chemo-sensitivity Mitochondrial biogenesis

Identification and quantitative estimation of Emodin in Rhei Emodi *** and the relevant polyherbal ayurvedic formulation

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Asian Journal of Traditional Medicines 2021年第6期16卷 351-366页

作者： Ajay Kumar Meena R.Ilavarasan Arjun Singh Lavkush Sharma amit dixit N.Srikanth Regional Ayurveda Research Institute AamkhoGwalior 474009India Captain Srinivasa Murthy Central Ayurveda Research Institute Chennai 600106India Central Ayurveda Research Institute Kolkata 700091India Central Council for Research in Ayurvedic Sciences Ministry of AYUSHGovernment of IndiaNew Delhi 110058India

The present study explored quality control of plant drug and polyherbal formulation using HPTLC and HPLC *** study combined qualitative analysis of Emodin and Rhein biomarker compound in Rhei Emodi *** extract and polyherbal formulation extract by HPTLC method with quantitative estimation of Emodin by HPLC method,aiming to help in accurate identification of Emodin and Rhein in plant materials,polyherbal *** study also checked adulteration,with the purpose of improving bio-efficacy of ayurvedic *** the samples showed characteristic peaks of Emodin at same retention time as that of standard *** to the HPTLC fingerprinting results,the Rf values of Rhein reference standard at 0.42 and Emodin at 0.62 were observed in test solution of Rhei emodi *** extract and polyherbal formulation extract and both were found *** quantitative evaluation of Emodin present in the polyherbal formulation and Rhei emodi *** extracts were 0.0177% and 0.3106% respectively.

关键词： Rhei emodi Emodin Rhein Standardization HPTLC HPLC and quantification

Fake News Classification Using a Fuzzy Convolutional Recurrent Neural Network

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Computers, Materials & Continua 2022年第6期71卷 5733-5750页

作者： Dheeraj Kumar dixit amit Bhagat Dharmendra Dangi Department of Mathematics and Computer Applications Maulana Azad National Institute of Technology(MANIT)BhopalMadhya PradeshIndia

In recent years,social media platforms have gained immense *** a result,there has been a tremendous increase in content on social media *** content can be related to an individual’s sentiments,thoughts,stories,advertisements,and news,among many other content *** the recent increase in online content,the importance of identifying fake and real news has ***,there is a lot of work present to detect fake news,a study on Fuzzy CRNN was not explored into this *** this work,a system is designed to classify fake and real news using fuzzy *** initial feature extraction process is done using a convolutional recurrent neural network(CRNN).After the extraction of features,word indexing is done with high ***,based on the indexing measures,the ranking process identifies whether news is fake or *** fuzzy CRNN model is trained to yield outstanding resultswith 99.99±0.01%*** work utilizes three different datasets(LIAR,LIAR-PLUS,and ISOT)to find the most accurate model.

关键词： Fake news detection text classification convolution recurrent neural network fuzzy convolutional recurrent neural networks

Sentiment Analysis on Social Media Using Genetic Algorithm with CNN

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Computers, Materials & Continua 2022年第3期70卷 5399-5419页

作者： Dharmendra Dangi amit Bhagat Dheeraj Kumar dixit Department of Mathematics and Computer Applications Maulana Azad National Institute of Technology(MANIT)BhopalMadhya PradeshIndia

There are various intense forces causing customers to use evaluated data when using social media platforms and microblogging ***,customers throughout the world share their points of view on all kinds of topics through these *** massive volume of data created by these customers makes it impossible to analyze such data ***,an efficient and intelligent method for evaluating social media data and their divergence needs to be ***,various types of equipment and techniques are available for automatically estimating the classification of *** analysis involves determining people’s emotions using facial *** analysis can be performed for any individual based on specific *** present study describes the analysis of an image dataset using CNNswithPCA intended to detect people’s sentiments(specifically,whether a person is happy or sad).This process is optimized using a genetic algorithm to get better ***,a comparative analysis has been conducted between the different models generated by changing the mutation factor,performing batch normalization,and applying feature reduction using *** steps are carried out across five experiments using *** maximum accuracy obtained is 96.984%,which is associated with the Happy and Sad sentiments.

关键词： Sentiment analysis convolutional neural networks facial expression genetic algorithm

Gall bladder carcinoma: Aggressive malignancy with protean loco-regional and distant spread

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World Journal of Clinical Cases 2015年第3期3卷 231-244页

作者： amit Nandan Dhar Dwivedi Shivi Jain Ruhi dixit Department of Radiodiagnosis and Imaging Institute of Medical Sciences Banaras Hindu University Department of General Surgery Institute of Medical Sciences Banaras Hindu University

The most common malignancy of biliary tract is gallbladder cancer(GBC) which is the third most common cancer in gastrointestinal tract. It is a lethal disease for most patients in spite of growing awareness and improved diagnostic techniques. GBC has a verypoor prognosis and the 5 year survival rate is < 10%. Although etiology of the carcinoma of the gallbladder is still obscure, various factors have been implicated, cholelithiasis being the most frequent. The incidence of GBC worldwide is based on the gender, geography and ethnicity which suggest that both genetic and environmental factors can cause GBC. The major route of spread of gallbladder cancer(GC) is locoregional rather than distant. It spreads by lymphatic, vascular, neural, intraperitoneal, and intraductal routes. Sonography is usually the most common imaging test to evaluate symptoms of biliary tract disease including suspected GC. With recent advances in imaging modalities like multi-detector computed tomography(CT) scanners, magnetic resonance imaging-positron emission tomography/CT diagnosis of gallbladder cancer has improved. Studies have also targeted molecular and genetic pathways. Treatment options have included extended and radical surgeries and adjuvant chemotherapy. This review article deals in detail with important aspects of carcinoma gallbladder and its manifestations and challenges. Role of various imaging modalities in characterization and accurate staging has been discussed. The loco-regional spread of this aggressive malignancy is dealt explicitly.

关键词： Gallbladder cancer Loco-regional and distant spread Cholelithiasis Imaging Adenocarcinoma

Application of statistical learning theory for thermodynamic modeling of natural gas hydrates

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Petroleum 2021年第4期7卷 502-508页

作者： Anupama Kumari Mukund Madhaw C.B.Majumder amit Arora Gaurav dixit Department of Chemical Engineering Indian Institute of TechnologyRoorkeeIndia DHC Trading India Private LimitedDelhiIndia Department of Chemical Engineering Shaheed Bhagat Singh State UniversityFerozepurPunjabIndia Gas Hydrate Research and Technology Centre PanvelMumbaiIndia

The gas hydrate formation in pipelines of industries and chemical plants can cause various operational damages and can increase economic ***,the knowledge of hydrate formation conditions has become a critical research study to overcome the problems arising from the formation of *** this study,we applied an algorithm to develop an LSSVM model to predict the formation temperature of natural gas hydrate for a comprehensive range of data *** 188 experimental data points were applied from the literature for the development of the LSSVM *** input parameter was finalized based on the structure of hydrates by each gas *** results obtained by the LSSVM model have good accuracy as compared with empirical correlations available in the *** model gave the squared correlation coefficient(R2),and root mean square error of 0.9901 and 0.59974,*** composition of gases may affect the phase equilibrium condition of gas *** applied algorithm revealed that the developed LSSVM model could become a good alternative for calculating the formation temperature of hydrate for the range of all data *** results showed that the proposed LSSVM model could be applicable for the prediction of hydrate formation temperature for all data points.

关键词： Gas hydrate LSSVM Correlation Hydrate formation temperature

Untargeted secondary metabolite profiling and putative pathway deduction of a marine Ochrophyte-Iyengaria stellata(Børgesen)using liquid chromatography-mass spectrometry

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Food Bioscience 2023年第1期51卷 1345-1360页

作者： Dhara dixit Coastal and Marine Ecology Division Gujarat Institute of Desert Ecology(GUIDE)Bhuj-370001 KachchhGujaratIndia Department of Earth and Environmental Science Krantiguru Shyamji Krishna Verma Kachchh UniversityBhuj-370001 KachchhGujaratIndia

This study divulges biochemical traits,mineral makeup,and the secondary metabolite outline of the less explored Ochrophyte,Iyengaria stellata(Børgesen)using untargeted metabolite profiling along the Pingleshwar coast,Kachchh,*** mineral Na(4921.33±0.577 mg/100 g Dry Weight)and micro mineral Fe(859.76±0.98 mg/100 g DW)were found to be in the highest quantities.A total of 108 putative metabolites were detected using both,the positive electrospray ionization mode(+ESI)and negative electrospray ionization mode(-ESI of the Liquid Chromatography-Mass Spectrometry-Time of Flight(LCMS-TOF).The identified metabolites included steroids,terpenoids,ketones,esters,polyphenols,anthraquinones,tocopherol,*** metabolomics data obtained from the+ESI mode led to the inference of a putative glycerophospholipid metabolism pathway that shall provide valuable insights for future metabolic engineering *** study highlights the first attempt to decipher the secondary metabolite composition of *** thereby further accounting for its potential as a natural source in pharmaceutical and nutraceutical compositions.

关键词： Iyengaria stellata Metabolomics Liquid chromatography-mass spectrometry Seaweed Untargeted metabolomics

Single Molecule Analysis of the Arabidopsis FRA1 Kinesin Shows that It Is a Functional Motor Protein with Unusually High Processivity

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Molecular Plant 2011年第5期4卷 879-885页

作者： Chuanmei Zhu Ram dixit Biology Department Washington University St Louis MO 63130 USA

The Arabidopsis FRA1 kinesin contributes to the organization of cellulose microfibrils through an unknown mechanism. The cortical localization of this kinesin during interphase raises the possibility that it transports cell wallrelated cargoes along cortical microtubules that either directly or indirectly influence cellulose microfibril patterning. To determine whether FRA1 is an authentic motor protein, we combined bulk biochemical assays and single molecule fluorescence imaging to analyze the motor properties of recombinant, GFP-tagged FRA1 containing the motor and coiled-coil domains （designated as FRAI（707）-GFP）. We found that FRAI（707）-GFP binds to microtubules in an ATP-dependent manner and that its ATPase activity is dramatically stimulated by the presence of microtubules. Using single molecule studies, we found that FRAI（707）-GFP moves processively along microtubule tracks at a velocity of about 0.4 μm s-1. In addition, we found that FRAI（707）-GFP is a microtubule plus-end-directed motor and that it moves along microtubules as a dimer. Interestingly, our single molecule analysis shows that the processivity of FRAI（707）-GFP is at least twice the processivity of conventional kinesin, making FRA1 the most processive kinesin to date. Together, our data show that FRA1 is a bona fide motor protein that has the potential to drive Iong-distance transport of cargo along cortical microtubules.

关键词： Kinesin FRA1 cortical microtubule single molecule cellulose Arabidopsis.

Partial genomic organization of ribosomal protein S7 gene from malaria vector Anopheles stephensi

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Insect Science 2007年第2期14卷 101-106页

作者： RAJNIKANT dixit SARITA dixit UPAL ROY YOGESH S.SHOUCHE SURENDRA GAKHAR Department of Biosciences Maharshi Dayanand University RohtakPune University Campus Pune India National Centre for Cell Science Pune University Campus Pune India

In this study, we describe the partial genomic organization of ribosomal protein S7 gene isolated from the mosquito Anopheles stephensi. Initially a 558 bp partial cDNA sequence was amplified as precursor mRNA sequence containing 223 bp long intron. 5＇ and 3＇ end sequences were recovered using end specific rapid amplification of cDNA ends （RACE） polymerase chain reaction. The full-length cDNA sequence was 914 nucleotide long with an open reading frame capable of encoding 192 amino acid long protein with calculated molecular mass of 22174 Da and a pI point of 9.94. Protein homology search revealed 〉75% identity to other insect＇s S7 ribosomal proteins. Analysis of sequence alignment revealed several highly conserved domains, one of which is related to nuclear localization signal （NLS） region of human rpS7. Interestingly, intron nucleotide sequence comparison with A. gambiae showed a lesser degree of conservation as compared to coding and untranslated regions. Like this, early studies on the genomic organization and cDNA/ Expressed sequence tag analysis （EST） could help in genome annotation ofA. stephensi, and would be likely to be sequenced in the future.

关键词： Anopheles malaria mosquito ribosomal protein vector