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中国晶体学会第六届学术年会暨会员代表大会（大分子晶体学分会）

作者： Qinyu Zhu David Venzke ameya s.walimbe Mary E.Anderson Qiuyu Fu Lisa Kinch Wei Wang Xing Chen Nick Grishin Niu Huang Liping Yu Jack E.Dixon Kevin P.Campbell Junyu Xiao The state Key Laboratory of Protein and Plant Gene Research School of Life SciencesAcademy for Advanced Interdisciplinary StudiesPeking-Tsinghua Center for Life SciencesPeking University Howard Hughes Medical Institute Department of Molecular Physiology and BiophysicsDepartment of NeurologyDepartment of Internal MedicineUniversity of Iowa Roy J.and Lucille A.Carver College of Medicine National Institute of Biological sciences Beijing Department of Biophysics University of TexasSouthwestern Medical Center Beijing National Laboratory for Molecular sciences College of Chemistry and Molecular EngineeringPeking-Tsinghua Center for Life SciencesSynthetic and Functional Biomolecules CenterKey Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationPeking University Medical Nuclear Magnetic Resonance Facility University of Iowa Roy J.and Lucille A.Carver College of Medicine Department of Pharmacology Department of Cellular and Molecular MedicineDepartment of Chemistry and BiochemistryUniversity of California

The"pseudokinase"sg K196 is a protein O-mannose kinase(POMK)that catalyzes an essential phosphorylation step during biosynthesis of the laminin-binding glycan ***,the catalytic mechanism underlying this activity remains *** we present the crystal structure of Danio rerio POMK in complex with Mg ions,ADP,aluminum fluoride,and

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New insights into estrogenic regulation of O^6-methylguanine DNA-methyltransferase (MGMT) in human breast cancer cells： Co-degradation of ER-α and MGMT proteins by fulvestrant or O^6-benzylguanine indicates fresh avenues for therapy

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The Journal of Biomedical Research 2016年第5期30卷 393-410页

作者： ameya Paranjpe Nathan I. Bailes santhi Konduri George C. Bobustuc Francis Ali-Osman Mohd. A. Yusuf surendra R. Punganuru Hanumantha Rao Madal Debasish Basak AGM Mostofa Kalkunte s. srivenugopa Department of Biomedical sciences and Cancer Biology Center School of Pharmacy Texas Tech University Health Sciences Center Amarillo TX 79106 USA Neuro-Oncology section Aurora Advanced Cancer Care Milwaukee WI 53215 USA Department of surgery The Preston Robert Tisch Brain Tumor Center Duke University Durham NC 27710 USA

Endocrine therapy using estrogen receptor-u （ER-α） antagonists for attenuating horm2one-driven cell proliferation is a major treatment modality for breast cancers. To exploit any DNA repair deficiencies associated with endocrine therapy, we investigated the functional and physical interactions of ER-α with O^6-methylguanine DNA methyltransferase （MGMT）, a unique DNA repair protein that confers tumor resistance to various anticancer alkylating agents. The ER-α -positive breast cancer cell lines （MCF-7, T47D） and ER- negative cell lines （MDAMB- 468, MDAMB-231）, and established inhibitors of ER-α and MGMT, namely, ICI-182,780 （Faslodex） and O^6- benzylguanine, respectively, were used to study MGMT- ER interactions. The MGMT gene promoter was found to harbor one full and two half estrogen-responsive elements （EREs） and two antioxidant-responsive elements （AREs）. MGMT expression was upregulated by estrogen, downregulated by tamoxifen in Western blot and promoter-linked reporter assays. similarly, both transient and stable transfections of Nrf-2 （nuclear factor-erythroid 2-related factor-2） increased the levels of MGMT protein and activity 3 to 4-fold reflecting novel regulatory nodes for this dragresistance determinant. Of the different ER-α antagonists tested, the pure anti-estrogen fulvestrant was most potent in inhibiting the MGMT activity in a dose, time and ER-α dependent manner, similar to O^6-benzylguanine. Interestingly, fulvestrant exposure led to a degradation of both ER-α and MGMT proteins and O^6-benzylguanine also induced a specific loss of ER-a and MGMT proteins in MCF-7 and T47D breast cancer cells with similar kinetics. Immunoprecipitation revealed a specific association of ER-a and MGMT proteins in breast cancer cells. Furthermore, silencing of MGMT gene expression triggered a decrease in the levels of both MGMT and ER-a proteins. The involvement of proteasome in the drug-induced degradation of both proteins was also demonstrated. Fulvestrant enhanced th

关键词： estrogen signaling MGMT DNA repair ubiquitin-proteasome pathway breast cancer anti-estrogens

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Unique interstitial miRNA signature drives fibrosis in a murine model of autosomal dominant polycystic kidney disease

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World Journal of Nephrology 2018年第5期7卷 108-116页

作者： ameya Patil William E sweeney Jr Cynthia G Pan Ellis D Avner Children’s Research Institute Children’s’Hospital Health System of Wisconsin and the Medical College of WisconsinMilwaukeeWI 53226United States

AIM To delineate changes in miRNA expression localized to the peri-cystic local microenvironment(PLM) in an orthologous mouse model of autosomal dominant polycystic kidney disease(ADPKD)(mcwPkd1^(nl/nl)).METHODs We profiled miRNA expression in the whole kidney and laser captured microdissection(LCM) samples from PLM in mcwPkd1^(nl/nl)kidneys with Qiagen miscript 384 HC miRNA PCR arrays. The three times points used are:(1) post-natal(PN) day 21, before the development of trichrome-positive areas;(2) PN28, the earliest sign of trichrome staining; and(3) PN42 following the development of progressive fibrosis. PN21 served as appropriate controls and as the reference time point for comparison of miRNA expression *** LCM samples revealed three temporally upregulated miRNAs [2 to 2.75-fold at PN28 and 2.5 to 4-fold(P ≤ 0.05) at PN42] and four temporally downregulated miRNAs [2 to 2.75 fold at PN28 and 2.75 to 5-fold(P ≤ 0.05) at PN42]. Expression of twenty-six miRNAs showed no change until PN42 [six decreased(2.25 to 3.5-fold)(P ≤ 0.05) and 20 increased(2 to 4-fold)(P ≤ 0.05)]. Many critical miRNA changes seen in the LCM samples from PLM were not seen in the contralateral whole *** Precise sampling with LCM identifies miRNA changes that occur with the initiation and progression of renal interstitial fibrosis(RIF). Identification of the target proteins regulated by these miRNAs will provide new insight into the process of fibrosis and identify unique therapeutic targets to prevent or slow the development and progression of RIF in ADPKD.

关键词： Inflammation End-stage renal disease Cysts Autosomal dominant poly cystic kidney disease miRNA Renal interstitial fibrosis

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