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Insight into Genetic Diversity of Cultivated Lima Bean (Phaseolus lunatus L.) in Benin

American Journal of Molecular Biology 2023年第1期13卷 32-45页

作者： Rose Fernande Fagbédji Lambert Gustave Djedatin Chimène Nanoukon Geofroy Kinhoegbe amed havivi Hounnankpon Yédomonhan Clément Agbangla Laboratoire de Biologie Moléculaire et de Bioinformatique Appliquée à la Génomique (BIOGENOM) ENSBBA de Dassa-Zoumé Université Nationale des Sciences Technologies Ingénierie et Mathématiques (UNSTIM) Abomey Benin Ecole Normale Supérieure de l’Enseignement Technique (ENSET) de Lokossa Université Nationale des Sciences Technologies Ingénierie et Mathématiques (UNSTIM) Abomey Benin Laboratoire de Botanique et Ecologie Végétale Faculté des Sciences et Techniques (FAST) Université d’Abomey-Calavi (UAC) Abomey-Calavi Benin Département de Génétique et des Biotechnologies Faculté des Sciences et Techniques (FAST) Université d’Abomey-Calavi Abomey-Calavi Benin

Lima bean is a tropical and subtropical legume from the genus Phaseolus which is cultivated for its importance in food and in medicine, but which remains a Neglected and Underutilized Crop in Benin. Understanding the genetic diversity of a species’ genetic resources is useful for the establishment of appropriate conservation strategies and breeding programs and for sustainable use. We use 6 out of ten SSR markers to analyze the diversity and population structure of 28 Lima bean landraces collected in Benin. A total of 28 alleles with an average of 4.16 alleles per SSR were amplified. The Polymorphic Information Content value ranged from 0.079 to 0.680 with an average of 0.408. The analysis of population structure revealed three subpopulations. PCoA revealed three well-separated clusters among the analyzed accessions in accordance with the population structure results and the clustering based on the Neighbor-Joining tree. AMOVA showed highly significant (p = 0.001) diversity among and within populations. Hence, 32% of the genetic variation was distributed among the population and 68% was distributed within populations. A high PhiP value (0.321) was found between the three sub-subpopulations indicating a high genetic differentiation between these sub-subpopulations. By exhibiting the highest average number of alleles, Shannon-Weaver information and Shannon-Weaver diversity indices, and the highest mean number of private alleles, subpopulation 1 is the main gene pool of the analyzed collection. The present study is an important starting point for the establishment of appropriate conservation strategies and breeding programs for Lima bean genetic resources.

关键词： Phaseolus lunatus L. Benin Genetic Diversity SSR Markets Conservation

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Sensory Phenotypic and Molecular Identification of Aromatic Rice Accessions Cultivated in Benin

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Advances in Bioscience and Biotechnology 2024年第3期15卷 195-206页

作者： Chimène Nadège Mahoussi Nanoukon Koffi David Montcho Hambada Deless Edmond Fulgence Thiémélé Bignon Meyrix Pamela Franzel Loumedjinon Babatoundé Franel Carel Wenceslas Affolabi amed Sèmèvo havivi Kéllya Laurinzo Déguénon Grâce Finagnon Vitoekpon Arielle Bankole Lambert Gustave Djedatin Laboratory of Molecular Biology and Bioinformatics Applied to Genomics National School of Applied Biosciences and Bio-technologies National University of Science Technology Engineering and Mathematics Dassa-Zoumè Benin Laboratory of Molecular Biology and Bioinformatics Applied to Genomics National School of Applied Biosciences and Biotechnologies National University of Science Technology Engineering and Mathematics Dassa-Zoumè Benin Laboratory of Plant Horticultural and Forestry Sciences National University of Agriculture Kétou Benin Genetics Teaching and Research Unit Department of Biochemistry-Genetics Peleforo GON COULIBALY University Korhogo C&#244te d’Ivoire

Rice is one of the most widely cultivated cereals in the world, and its aroma is increasingly in demand. With the advancement of research, a major rice flavor gene has been identified on rice chromosome 8. It encodes non-functional betaine aldehyde dehydrogenase leading to the accumulation of 2-acetyl-1-pyrroline which is the major olfactory compound that confers the fragrant character to rice. The aroma of rice is considered a special trait of enormous economic importance that determines the prime price in world trade. To satisfy the needs of the population and reduce rice imports into Benin, we conducted this study to identify aromatic rice accessions grown in Benin. Seventy-two rice accessions collected across Benin were PCR amplified with three SSR markers RM 7049, Aro 7, and RM 223, linked to the fgr (fragrance of rice) aroma gene. Molecular analysis revealed that 12 of the 72 accessions, namely Bagou 19, Bagou 22, Tchaka 34, Foun 15, Tchaka 41, Nana 32, Kan 61, Kung 69, Kung 67, Bagou 20, Agbab 101 and Koum 55 possess the fgr gene and can be considered as aromatic rice accessions. A sensory phenotypic test using KOH was carried out on rice accessions carrying fgr gene. Of the twelve positives, only one had the smell of aromatic rice, like the Azucena control. These results show that Benin also has aromatic rice varieties that can be sold on national and international markets.

关键词： Aromatic Rice 2-Acetyl-1-Pyrroline SSR Markers Benin

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Omphalocele Diagnosis Problem in Rural Environment

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Surgical Science 2024年第8期15卷 501-506页

作者： Kanté Sékou Diallo Siaka Touré Cheick amed Sékou Ousmane I. Touré Coulibaly Ibrahim Traoré Drissa Bengaly Brehima Sanogo Soulemane Togola Birama Ouattara Drissa Saye Jack Ongoïba Nouhoum Koumaré Abdel Karim Service de Chirurgie B CHU Point G Bamako Mali Facult de Mdecine de lUSTTB Bamako Mali

Introduction: An omphalocele is a congenital malformation due to a failure of closure of the umbilical ring with exteriorization of the abdominal viscera covered by the translucent and avascular amniotic membrane. We report a case of giant omphalocele unrecognized at birth followed by a review of the literature. Observation: This was a 4-year-old girl admitted to the department for umbilical swelling that had been present since birth. Clinical and paraclinical examinations made it possible to objectivize a post-omphalocele hernia with hepatic content plus ectopic accession of the left ureter, the surgical procedure consisted of reintroducing the liver associated with a cure of the hernia + ureterovesical reimplantation. Conclusion: Omphalocele is a congenital malformation, antenatal diagnosis is possible during morphological ultrasound from the 12th week.

关键词： Omphalocele Ectopic Left Ureter

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Phytochemical and antioxidant activities of Rumex crispus L. in treatment of gastrointestinal helminths in Eastern Cape Province, South Africa

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Asian Pacific Journal of Tropical Biomedicine 2017年第12期7卷 1071-1078页

作者： Oladayo amed Idris Olubunmi Abosede Wintola Anthony Jide Afolayan Medicinal Plants and Economic Development Research Centre Department of BotanyUniversity of Fort HareAlice5700South Africa

Objective: To evaluate the antioxidant activities and phytochemical content of the leaf and root extracts of Rumex crispus using the solvents extraction; methanol extract,ethanol extract, acetone extract(ACE), and water ***: Total flavonoids content, total phenolic content, and total proanthocyanidin were evaluated using spectrophotometric equivalents of the standards, quercetin, gallic acid and catechin respectively. The antioxidant activities of the plant extracts were determined using ABTS, DPPH, ferric reducing antioxidant power, total antioxidant capacity and nitric oxide scavenging ***: The flavonoids and phenols contents of the extracts were in the range of(19.39 ± 4.08) to(526.23 ± 17.52) mg QE/g and(16.95 ± 12.03) to(240.68 ± 3.50) mg GAE/g, respectively. ACE of the leaf has the highest value of total flavonoids content(526.23 ± 17.52) mg QE/g while ACE of the root has the highest value of total phenolic content(240.68 ± 3.50) mg GAE/g. The highest content of total proanthocyanidin(645.38 ± 1.33) mg CE/g was in ACE of the root. Significant amounts of saponin and alkaloid were also present in the root and leaf extracts. All solvent fractions showed significant antioxidant activities(P < 0.05) with ACE of the root having the highest scavenging value as shown in DPPH, ABTS, total antioxidant capacity, nitric oxide and ferric reducing antioxidant power(IC50= 0.014 mg/m L, <0.005 mg/m L, 0.048 mg/m L,0.067 mg/m L, and 0.075 mg/m L, respectively).Conclusions: In this study, the mean phytochemical content of the root of Rumex crispus is higher than that of the leaf and this may have contributed to its high antioxidant activities. This may also justify the frequent use of the root more than the leaves in traditional medicine for the cure of helminthic infections.

关键词： Antioxidant Phytochemical Rumex crispus Helminths Treatment Scavenge

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Farm Technical Efficiency under the Seasonal Milk Production of the Dual-Purpose Cattle System in El Salvador

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Journal of Agricultural Science and Technology(A) 2014年第3期4卷 266-275页

作者： Wen-Chi Huang Angel amed Duron-Benitez Graduate Institute of Agribusiness Management National Pingtung University of Science and Technology Pingtung 912 TaiwanRepublic of China Department of Tropical Agriculture and International Cooperation National Pingtung University of Science and TechnologyPingtung 912 Taiwan Republic of China

The objective of this research was to calculate the technical efficiency and its determinants in seasonal milk production of dual-purpose cattle system farms of El Salvador （DPCS）. Seasons were classified as rainy （May-October） and dry （November-April）. The stochastic production frontier model through the Cobb-Douglas specification was used to provide the technical efficiency score and its determinants for the production data of the 2009 rainy season and the 2010 dry season of the DPCS farms from South East El Salvador. The results showed that the mean technical efficiency was 14% higher for the dry season than for the rainy season. Moreover, more farms obtained higher efficiency scores in the dry season. In the rainy season, milk production was attributed to labor and farmland, whereas in the dry season, it was attributed to the decrease of herd size. Therefore, we suggest adopting strategies such as increased cow productivity and improved feed quality to increase the overall technical efficiency of DPCS farms.

关键词： Dual-purpose cattle system technical efficiency rainy and dry season milk seasonality.

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访谈高端

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中国制衣 2014年第7期 62-63页

作者： Imran amed

McArthurGlen奥特莱斯专业的客户服务和全方位的设施也是消费者所津津乐道的。外加令人目不暇接的精彩活动,包括时装秀,现场音乐会,美食节等等,全年免费对顾客开放。McArthurGlen的首席执行官Julia Calabrese说:"我们设计的名品奥特莱... 详细信息

McArthurGlen奥特莱斯专业的客户服务和全方位的设施也是消费者所津津乐道的。外加令人目不暇接的精彩活动,包括时装秀,现场音乐会,美食节等等,全年免费对顾客开放。McArthurGlen的首席执行官Julia Calabrese说:"我们设计的名品奥特莱斯中心极为关注品质和细节。我们要让顾客们享受购物时光,这都体现在我们的建筑、设施和举办的活动中,当然还有我们出类拔萃的合作品牌。"奥特莱斯还以特别优惠的价格增加了对游客的吸引力。游客在购买称心的名品时可享受30-70%折扣,买够固定金额还能享受到退税优惠。

关键词：奥特莱斯美国欧洲美利坚合众国北美洲奢侈品牌俄罗斯消费额旅游者游客

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Cristophe Chenut讲述Lacoste品牌背后的传奇

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中国制衣 2012年第9期 66-69页

作者： Imran amed

拥有标志性的产品,是一个强势,同时也是一个弱点。无数的顾客到专卖店买一种标志性产品,这对于品牌来说是一个无可比拟的机遇。但是要想让这些顾客去关注其他的产品就成了一件困难的事情,因为他们就想购买polo衫。所以我们不得不投入更... 详细信息

拥有标志性的产品,是一个强势,同时也是一个弱点。无数的顾客到专卖店买一种标志性产品,这对于品牌来说是一个无可比拟的机遇。但是要想让这些顾客去关注其他的产品就成了一件困难的事情,因为他们就想购买polo衫。所以我们不得不投入更多以确保标志性的产品能保持领先的地位,与此同时,还要吸引消费者去关注店面里的其他产品,比如裤子或者夹克衫。

关键词：品牌 Cristophe Chenut Lacoste

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Antenatal use of bosentan and/or sildenafil attenuates pulmonary features in rats with congenital diaphragmatic hernia

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World Journal of Pediatrics 2014年第4期10卷 354-359页

作者： María de Lourdes Lemus-Varela amed Soliz Belinda Claudia Gómez-Meda Ana Lourdes Zamora-Perez José Manuel Ornelas-Aguirre Valery Melnikov Blanca Miriam Torres-Mendoza Guillermo Moisés Zúñiga-González Division of Neonatology High Specialty Medical UnitPediatric HospitalWestern National Medical CenterMexican Social Security InstituteGuadalajaraJaliscoMéxico Neonatal Intensive Care Unit Division of NeonatologyMiami Children's HospitalMiami FLDUSA Molecular Biology in Medicine Institute Molecular Biology and Genomic DepartmentHealth Sciences University CenterUniversity of GuadalajaraGuadalajaraJaliscoMéxico Odontology Research Institute Integrated Odontology Clinic DepartmentHealth Sciences University CenterUniversity of GuadalajaraGuadalajaraJaliscoMéxico Pathology Department High Specialty Medical UnitNorth-western National Medical CenterMexican Social Security InstituteCiudad ObregónSonoraMéxico School of Medicine University of ColimaColimaMéxico Division of Neurosciences Western Biomedical Research CenterMexican Social Security Institute and Department of ClinicsHealth Sciences University CenterUniversity of GuadalajaraGuadalajaraJaliscoMéxico Mutagenesis Laboratory Western Biomedical Research CenterMexican Social Security InstituteGuadalajaraJaliscoMéxico

Background:Lung hypoplasia,pulmonary persistent hypertension of the newborn and its morphological changes are the main features in congenital diaphragmatic hernia(CDH).This study was undertaken to investigate if antenatal use of sildenafil and/or bosentan attenuates vascular remodeling,promotes branching,and improves alveolarization in experimental nitrofeninduced ***:Nitrofen(100 mg)was gavage-fed to pregnant rats at post conception day(PCD)9 to induce *** rats were randomized to 5 groups:1)control;2)nitrofen;3)nitrofen+sildenafil 100 mg/kg per day at PCD 16-20;4)nitrofen+bosentan 30 mg/kg per day,at PCD 16-20,and 5)nitrofen+bosentan+sildenafil,same doses and administration *** cesarean delivery,the offsprings were sacrifi *** diaphragmatic defect and pulmonary hypoplasia were identified,and the lungs were *** wall thickness,bronchiolar density and alveolarization were ***:The offsprings with CDH were characterized by severe pulmonary hypoplasia(lung weight-to-body weight ratio:0.0263[95%confidence interval(CI)0.0242-0.0278)]in the nitrofen group versus 0.0385(95%CI 0.0355-0.0424)in the control group(P=0.0001).Pulmonary arterial wall thickness was decreased to 3.0(95%CI 2.8-3.7)μm in the nitrofen+sildenafil group versus 5.0(95%CI 4.1-4.9)μm in the nitrofen group(P=0.02).Terminal bronchioles increased to 13.7(95%CI 10.7-15.2)μm in the nitrofen+bosentan group in contrast to 8.7(95%CI 7.2-9.4)μm in the nitrofen group(P=0.002).More significant differences(P=0.0001)were seen in terminal bronchioles in the nitrofen+sildenafil+bosentan group than in the nitrofen group[14.0(95%CI 12.5-15.4)μm versus 8.5(95%CI 7.1-9.3)μm].Pulmonary arterial wall thickness was also decreased in the former ***:In this rat model,antenatal treatment with sildenafil attenuates vascular *** promotes the development of terminal bronchioles in nitrofen-induced CDH.

关键词： antenatal treatment bosentan congenital diaphragmatic hernia pulmonary hypoplasia sildenafil

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追寻经典品牌Zegna新征程访Ermenegildo Zegna集团CEO Gildo Zegna

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中国制衣 2013年第8期 68-71页

作者：张弛 Imran amed

6月22日，Zegna（杰尼亚）在米兰时装周举行了2014春夏Couture高级成衣系列新品发布会，这是前YvesSaintLauent（圣罗兰）创意总监StefanoPilati自去年9月被任命为Zegna新一任创意总监以来的首次亮相，并赢得了时尚界的一致好评。业界... 详细信息

6月22日，Zegna（杰尼亚）在米兰时装周举行了2014春夏Couture高级成衣系列新品发布会，这是前YvesSaintLauent（圣罗兰）创意总监StefanoPilati自去年9月被任命为Zegna新一任创意总监以来的首次亮相，并赢得了时尚界的一致好评。业界盛赞此次StefanoPilati的设计弥漫着丰富、悠闲的剪裁手法，恰到好处地表现了传统与现代之间的完美平衡，而其个人风格的设计与Zegna品牌的特色实现了无缝连接。

关键词：品牌新品发布会米兰时装周无缝连接个人风格圣罗兰总监创意

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重回Made In Italy经典的Fendi 访Fendi集团CEO Pietro Beccari

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中国制衣 2014年第6期 64-67页

作者： Imran amed

Pietro Beccari在两年前出任著名奢侈品牌Fendi的CEO,暗示着他在LVMH这个奢侈品帝国中的位置正在不断上升,成为集团主席Bernard Arnault的得力助手。前不久,他出现在英国伦敦新邦德街,在那里Fendi一家面积达到630平方米的全新旗舰店隆... 详细信息

Pietro Beccari在两年前出任著名奢侈品牌Fendi的CEO,暗示着他在LVMH这个奢侈品帝国中的位置正在不断上升,成为集团主席Bernard Arnault的得力助手。前不久,他出现在英国伦敦新邦德街,在那里Fendi一家面积达到630平方米的全新旗舰店隆重开业。

关键词：服装行业服装设计制衣品牌时尚

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