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American Journal of Plant Sciences 2016年第3期7卷 500-509页

作者： Tanguturi Venkata Kirankumar Kalaiahgari Venkata Madhusudhan ambekar nareshkumar Kurnool Kiranmai Uppala Lokesh Boya Venkatesh Chinta Sudhakar Plant Molecular Biology Laboratory Department of Botany Sri Krishnadevaraya University Anantapuramu India

Foxtail millet (Setaria italica L.) is a drought-tolerant millet crop of arid and semi-arid regions. Aldo-keto reductases (AKRs) are significant part of plant defence mechanism, having an ability to confer multiple stress tolerance. In this study, AKR1 gene expression was studied in roots and leaves of foxtail millet subjected to different regimes of PEG- and NaCl-stress for seven days. The quantitative Real-time PCR expression analysis in both root and leaves showed upregulation of AKR1 gene during PEG and salt stress. A close correlation exits between expression of AKR1 gene and the rate of lipid peroxidation along with the retardation of growth. Tissue-specific differences were found in the AKR1 gene expression to the stress intensities studied. The reduction in root and shoot growth under both stress conditions were dependent on stress severity. The level of lipid peroxidation as indicated by MDA formation was significantly increased in roots and leaves along with increased stress levels. Finally, these findings support the early responsive nature of AKR1 gene and seem to be associated at least in part with its ability to contribute in antioxidant defence related pathways which could provide a better protection against oxidative stress under stress conditions.

关键词： Aldo-Keto Reductase Lipid Peroxidation Reactive Carbonyls Cellular Homeostasis Plant Abiotic Stress Response

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (<i>Arachis hypogaea</i>L.) Cultivars

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Agricultural Sciences 2015年第10期6卷 1283-1297页

作者： ambekar nareshkumar Gounipalli Veeranagamallaiah Merum Pandurangaiah Kurnool Kiranmai Vennapusa Amaranathareddy Uppala Lokesh Boya Venkatesh Chinta Sudhakar Plant Molecular Biology Unit Department of Botany Sri Krishnadevaraya University Anantapur India

Lead (Pb) is an important environmental pollutant extremely toxic to plants and other living organisms including humans. To assess Pb phytotoxicity, a pot culture experiment was carried out using two groundnut cultivars (Arachis hypogaea L. cultivar K6 and cultivar K9) on plant growth, ROS levels, lipid peroxidation, and antioxidant metabolism using biochemical, histochemical methods. Plants were grown in pots for 14 days, in the botanic garden, and subjected to Pb-stress (0, 100, 200, 400 and 800 ppm) by adding Pb (NO3)2 solution and further allowed to grow for 10 days. The results showed that cultivar K6 registered lower Pb accumulation than cultivar K9, however, localization of Pb was greater in roots than leaves in both groundnut cultivars. The Pb-stress results in an increase in free radicals (O2?- and H2O2) generation in both groundnut cultivars, but more significantly in cultivar K9 than K6. Pb-stress also caused significant changes in the rate of peroxidation as shown in the levels of malondialdehyde (MDA) content in roots and leaves of both groundnut cultivars. Free proline, ascorbic acid (AsA) and non-protein thiol (NP-SH) contents were increased in cultivar K6 due to Pb-stress, but less in cultivar K9. Pb treated plants showed increased levels of antioxidant enzymes such as superoxide dismutase (SOD), guaiacol peroxidase (GPX), ascorbate peroxidase (APX) glutathione reductase (GR) and glutathione S-transferase (GST). Isozyme band intensities of SOD, GPX and APX were more consistent with the respective changes in antioxidative enzyme activities. These results indicate that cultivar K6 possesses greater tolerance potential for Pb toxicity than cultivar K9.

关键词： Groundnut (Arachis hypogaea L.) Pb-Stress Oxidative Stress Antioxidant Enzymes Non-Protein Thiols Reactive Oxygen Species

Expression Analysis of WRKY Transcription Factor Genes in Response to Abiotic Stresses in Horsegram (Macrotyloma uniflorum (Lam.) Verdc.)

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American Journal of Molecular Biology 2016年第4期6卷 125-137页

作者： Kurnool Kiranmai Lokanadha Rao Gunupuru ambekar nareshkumar Vennapusa Amaranatha Reddy Uppala Lokesh Merum Pandurangaiah Boya Venkatesh Tanguturi Venkata Kirankumar Chinta Sudhakar Department of Botany Sri Krishnadevaraya University Anantapur India Molecular Plant-Microbe Interactions Laboratory University College Dublin Dublin Ireland Department of Crop Physiology GKVK Bangalore India Shenzhen Research Institute The Chinese University of Hong Kong Shenzhen China

Drought and salt stress are two major environmental constraints that limit the productivity of agriculture crops worldwide. WRKY transcription factors are the plant-specific transcription factors that regulate several developmental events and stress responses in plants. The WRKY domain is defined by a 60-amino acid conserved sequence named WRKYGQK at N-terminal and a Zinc Finger-like motif at the C-terminal. WRKY genes are known to respond several stresses which may act as negative or positive regulators. The function of most of the WRKY transcription factors from non-model plants remains poorly understood. This investigation shows the expression levels of eight WRKY transcription factor genes from horsegram plant under drought and salt stress conditions. The increase in mRNA transcript levels of WRKY transcription factor genes was found to be high in drought stressed plants compared to salt-stressed plants. The levels of MDA which indicates the lipid peroxidation were less in drought stress. More ROS is produced in salt stress conditions compared to drought. The results show that the expression of WRKY transcription factors in drought stress conditions is reducing the adverse effect of stress on plants. These results also suggest that, during abiotic stress conditions such as drought and salt stress, WRKY transcription factors are regulated at the transcription level.

关键词： Drought Salt Lipid Peroxidation Plant Abiotic Stress qRT-PCR Reactive Oxygen Species WRKY

The Use of Dried Blood Spot Samples in Screening Drugs of Abuse

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Pharmacology & Pharmacy 2013年第2期4卷 152-159页

作者： Rizwana Quraishi Raka Jain Atul ambekar National Drug Dependence Treatment Centre Department of Psychiatry All India Institute of Medical Sciences New Delhi India

The present article will provide an overview of use of dried blood sampling method for analysis of drug of abuse. Relatively short half life and instability in blood, calls for alternative sampling method for determination of drugs of abuse. Dried blood spot (DBS) method has many advantages over the conventional sampling methods. The available method for DBS sample collection, storage and transport are described here. The techniques involved in and the factors that may influence the accuracy and reproducibility of the DBS methods for determination of drugs of abuse are presented. The DBS sampling has the potential to be a useful technique to detect drugs of abuse. The use of DBS for any drug should be judged against the potential error involved with the method.

关键词： Dried Blood Spots Drugs of Abuse Screening Review

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世界著名沉船事件之二泰坦尼克号

海外英语 2006年第4期 8-9页

作者： Yogesh ambekar

　　Titanicthe name itself brings in front of us the picture of disaster of huge ship and sympathy1 for thousands of passengers on the Titanic who were drowned.……

关键词：世界著名沉船事件

鲤科鱼类遗传研究评述及遗传改良亚洲鲤科鱼类的途径

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国外水产 1991年第3期 12-15页

作者： ambekar E.Eknath 高健

亚洲有数千年养殖鲤科鱼类的历史,主要养殖品种有鲤(Cyprinus carpio)、草鱼(Ctenopharyngodon idella)、鳙(Aristichthys nobilis)、鲢(Hypophthalmichthys molitrix)、厚唇鲃(Catla carla)、印度野鲮(Labeo rohita)和印度鲮(Cirrhinus... 详细信息

关键词：鲤科鱼类遗传亚洲鲤科改良

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养殖罗非鱼的品种鉴定和遗传改良

国外水产 1993年第1期 22-24页

作者： ambekar E.Eknath 任维美山东省淡水水产研究所

菲律宾于1950年首次从泰国引入莫桑比克罗非鱼(Oreochromis mossambicus).由于此鱼生殖过早使池塘中的群体过剩未引起人们的养殖兴趣,故一直未形成商业化养殖.直到70年代引进了尼罗罗非鱼(Oreochromis niloticus)后。

关键词：罗非鱼品种鉴定遗传改良

脊髓髓内肿瘤手术病人的出院后处理、并发症和住院费用:2003-2010年美国全国住院病人样本数据分析

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中国微侵袭神经外科杂志 2013年第12期18卷 576-576页

作者： Sharma M Sonig A ambekar S 周静侯维娟

目的利用美国全国住院病人样本（NIS）数据库，对手术切除的脊髓髓内肿瘤病人的不良预后、住院病死率、总体并发症发生率、住院时间和费用进行分析。方法选取2003—2010年NIS同一组病人数据，回顾性分析多种病人相关变量（人口统计类别... 详细信息

目的利用美国全国住院病人样本（NIS）数据库，对手术切除的脊髓髓内肿瘤病人的不良预后、住院病死率、总体并发症发生率、住院时间和费用进行分析。方法选取2003—2010年NIS同一组病人数据，回顾性分析多种病人相关变量（人口统计类别、并发症、合并疾病和平均家庭收入）和医院相关变量（病床数、高／低收容量、位于农村／城市、区域、所有权、教学现状）。将不良处理、住院病死率以及较高的住院费用作为因变量。结果来自NIS数据库的15545例住院病人被纳入研究。

关键词：并发症发生率住院病人住院费用脊髓髓内肿瘤手术病人院后处理样本数据美国