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Ahmadea dalanensis *** ***.,an edible truffle from Pakistan

Studies in Fungi 2020年第1期5卷 452-461页

作者： aman.n.khalid an.mon.alvo jm Department of Botany University of the PunjabLahorePakistan Department of natural History Royal Ontario MuseumTorontoONCanada Department of Ecology and Evolutionary Biology University of TorontoTorontoONCanada

Ahmadea,a monotypic truffle genus is described morphologically and its position in the family Pezizaceae(Pezizales,Ascomycota)is inferred from the phylogenetic analyses of DnA sequences obtained from the large subunit nuclear ribosomal RnA gene(LSU).Its type species,***,is found in arid and semi-arid regions of Punjab,Pakistan,often occurring in Sorghum vulgare crop fields where it has been known for its edibility for *** of Ahmadea with the related truffle genera Stouffera Kovács&Trappe,Temperantia ***,Healy&Kovács,Hydnobolites Tul.&*** Delastria Tul.&*** discussed in this paper.

关键词： Ascomycota ITS paralogues LSU Pezizaceae Pezizales Sorghum Taxonomy

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The Effect of Media on Biomass and Oil Production in Botryococcus braunii Strains Kossou-4 and Overjuyo-3

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International Journal of Clean Coal and Energy 2015年第1期4卷 11-22页

作者： khalid A. Al-Hothaly Aidyn Mouradov Abdulatif A. Mansur Brian H. May Andrew S. Ball Eric M. Adetutu School of Applied Sciences RMIT University Bundoora Australia Department of Biotechnology Faculty of Science Taif University Taif Kingdom of Saudi Arabia Department of Environmental and natural Resources Engineering Faculty of Engineering Azawia University Azawia Libya School of Health Sciences RMIT University Bundoora Australia

The green algae Botryococcus braunii is widely recognized as a source of oil, including hydrocarbons. However, the slow rate of growth B. braunii hampers its commercial development. This stu- dy addresses this by examining the effects of three growth media on biomass and oil production in two B. braunii Race B strains, Kossou-4 and Overjuyo-3. Growth of B. braunii strains in BG11 medium resulted in significantly higher growth (2.3 - 4.2 and 2.9 - 6.0 fold increases in Kossou-4 and Overjuyo-3 respectively) compared to the jm and BBM-3n media after 15 days. A similar trend was obtained when biomass was measured indirectly using optical density (OD) and chlorophyll fluo-rescence. Oil production was also significantly higher in BG11 whether measured as oil weight or absorbance (ODs at 680 and 750 nm). However, the presence of extracellular oil was shown to in-crease absorbance values making OD measurements less reliable than dry weight assays. Maximum recovery of oil was recorded when hexane was used as solvent compared to hexane-isopro- panol and heptane. These results suggest that BG11 is the best growth medium for these two strains under the conditions of this experiment.

关键词： B. braunii Race B Media Biomass Oil Production

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