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Rodent hosts and flea vectors in Brazilian plague foci:a review

Integrative Zoology 2021年第6期16卷 810-819页

作者： Diego Leandro REIS DA SILVA FERNANdeS Matheus FILGUEIRA BEZERRA Marise SOBREIRA BEZERRA DA SILVA Nilma Cintra LEAL Christian Robson de SOUZA REIS alzira maria paiva de almeida Nacional Reference Service for Plague Institut Aggeu Magalhães-FIOCRUZ PERecifePEBrazil department of Microbiology Institut Aggeu Magalhães-FIOCRUZ PERecifePEBrazil

Plague,caused by the Yersinia pestis bacterium,has several foci scattered throughout a large area from the Brazilian territory that ranges from the Northeastern State of Cearáto the Southeastern State ofMinas Gerais and another separated area at the State of Rio de *** review gathers data from plague control and surveillance programs on the occurrence and geographic distribution of rodent hosts and flea vectors in the Brazilian plague areas during the period of from 1952 to ***,we discuss how the interaction between *** and some rodent host species may play a role in the disease *** absence of human cases nowadays in Brazil does not mean that it was *** dynamics of plague in Brazil and in other countries where it was introduced during the 3rd pandemic are quite alike,alternating epidemics with decades of ***,it remains an important epidemic disease of global *** existence of a large animal reservoir and competent vectors demonstrate a need for continuous surveillance to prevent new outbreaks of this disease in humans.

关键词： fleas plague rodents transmission vectors

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The development of a Loop-Mediated Isothermal Amplification (LAMP) Procedure for Plague Diagnostic

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American Journal of Analytical Chemistry 2014年第16期5卷 1069-1077页

作者： mariana de Lira Nunes Carina Lucena Mendes-Marques alzira maria paiva de almeida Nilma Cintra Leal departamento de Microbiologia Centro de Pesquisas Aggeu Magalh&atilde es FIOCRUZ-PE Recife Brazil

Plague caused by Yersinia pestis is one of the infectious diseases subject to the International Health Regulations (IHR). Permanent monitoring of the focal plague areas is mandatory in order to enable prompt control measures to prevent the spread of the disease. Therefore, the availability of efficient diagnosis tests is of paramount importance. Here, we describe a loop-mediated isothermal amplification (LAMP)-based procedure for rapid Y. pestis detection. We constructed a set of LAMP primers, which were used in assays to establish the reaction conditions that would lead to the quick visualization of the results by evaluating the test tube with the naked eye. The primers were specifically designed to target the caf1 gene located on pFra/Tox (pMT), a prototypical plasmid of Y. pestis. The LAMP procedure was performed at 65°C for 45 min in a water bath and allowed for the detection of at least 10 pg of bacterial DNA. Due to its simplicity, specificity, sensitivity and rapidity, the LAMP technique is an additional tool that may be implemented in routine plague diagnoses, especially in emergencies.

关键词： Plague Yersinia pestis Diagnosis Tests Loop-Mediated Isothermal Amplification (LAMP)

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Loop-Mediated Isothermal Amplification (LAMP) for the detection of <i>Listeria monocytogenes</i>and Major Pathogenic Serotypes

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American Journal of Analytical Chemistry 2014年第16期5卷 1057-1064页

作者： Ana Paula Rocha da Costa mariana de Lira Nunes Carina Lucena Mendes-Marques alzira maria paiva de almeida Nilma Cintra Leal departamento de Microbiologia Centro de Pesquisas Aggeu Magalh&atilde es FIOCRUZ-PE Recife Brazil

Rapid identification and characterization of Listeria monocytogenes are required for the food industry, epidemiological studies, and disease prevention and control. However, typing procedures are labor-intensive and time-consuming, and they require technical expertise, a panel of sera and reference culture strains or sophisticated and expensive equipment. To improve upon traditional diagnostic methods for L. monocytogenes we developed and evaluated an efficient procedure for the specific identification of L. monocytogenes and the major pathogenic serotypes of the species based on loop-mediated isothermal amplification (LAMP). Four individual reactions were designed using primers targeting any L. monocytogenes serotypes (LAMP-AS) and the 1/2a (LAMP-1/2a), 1/2b (LAMP-1/2b), and 4b (LAMP-4b) serotypes. The procedure distinguished L. monocytogenes from closely genetically related species and the targeted serotypes. Cross-reactivity with a few rare serotypes isolated from food or clinical samples did not impair the usefulness of the procedure. Thus, our approach constitutes a fast, easy and low-cost alternative for L. monocytogenes diagnosis and serotyping and may be useful for surveillance and epidemiological investigation programs.

关键词： Listeria monocytogenes Diagnosis Serotypes Identification LAMP

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Technique to Obtain Mitotic Chromosomes of <i>Conyza bonariensis</i>L. Cronquist (Asteraceae)

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American Journal of Plant Sciences 2015年第9期6卷 1466-1474页

作者： Juliana maria de Paula Cecília alzira Ferreira Pinto-Maglio Programa de Pós-Graduaç&atildeo em Genética Melhoramento Vegetal e Biotecnologia Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais Instituto Agron&ocircmico de Campinas Campinas Brazil Centro de Pesquisa e desenvolvimento de Recursos Genéticos Vegetais Instituto Agron&ocircmico de Campinas Campinas Brazil

Cytogenetic analysis requires cytological preparations that unequivocally reveal the chromosome number and permit optimal visualizations of chromosome morphology for the construction of karyotypes and ideograms. Chromosomal characterization is possible only by establishing these two parameters. To cytogenetically characterize the weed Conyza bonariensis (L.) Cronquist (Asteraceae), it was necessary to improve cytological analysis techniques to obtain optimal results. This species belongs to a genus whose plants have wide phenotypic plasticity. These plants can be morphologically differentiated by other types of analysis, and thus the application of this technique will serve as a reference for cytogenetical analysis of other groups of plants that have cytogenetic characteristics similar to those of C. bonariensis. The methodology described here highlights three main protocol steps: 1) root tip collection from newly germinated seed radicles and from young root tips of mature plants, 2) pretreatment of meristems with antimitotic agents, both isolated and combined, and 3) acid and enzymatic hydrolytic processes.

关键词： Hairy Fleabane Cytogenetics Chromosomes Weed

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