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Skin tissue engineering advances in severe burns:review and therapeutic applications

Burns & Trauma 2016年第1期4卷 13-26页

作者： alvin wen choong chua Yik Cheong Khoo Bien Keem Tan Kok Chai Tan Chee Liam Foo Si Jack Chong Singapore General Hospital Department of PlasticReconstructive andAesthetic Surgery20 College RoadAcademia Level 4Singapore 169845Singapore. Singapore General Hospital Skin Bank UnitBlock 4 Level 3Room 15Outram RoadSingapore 169608Singapore. Transplant TissueCentre c/o Skin Bank UnitSingapore General HospitalBlock 4 Level 3 RoomA7Outram RoadSingapore 169608Singapore. Singapore General Hospital Skin Bank UnitBlock 4 Level 3Room 15Outram RoadSingapore 169608Singapore. Transplant TissueCentre c/o Skin Bank UnitSingapore General HospitalBlock 4 Level 3 RoomA7Outram RoadSingapore 169608Singapore. Singapore General Hospital Department of PlasticReconstructive andAesthetic Surgery20 College RoadAcademia Level 4Singapore 169845Singapore. Singapore General Hospital Skin Bank UnitBlock 4 Level 3Room 15Outram RoadSingapore 169608Singapore.

Current advances in basic stem cell research and tissue engineering augur well for the development of improved cultured skin tissue substitutes:a class of products that is still fraught with limitations for clinical *** the ability to grow autologous keratinocytes in-vitro from a small skin biopsy into sheets of stratified epithelium(within 3 to 4 weeks)helped alleviate the problem of insufficient donor site for extensive burn,many burn units still have to grapple with insufficient skin allografts which are used as intermediate wound coverage after burn *** offered by tissue-engineered skin dermal replacements to meet emergency demand have been used fairly *** the availability of these commercial products,they all suffer from the same problems of extremely high cost,sub-normal skin microstructure and inconsistent engraftment,especially in full thickness *** practice for severe burn treatment has since evolved to incorporate these tissue-engineered skin substitutes,usually as an adjunct to speed up epithelization for wound closure and/or to improve quality of life by improving the functional and cosmetic results *** review seeks to bring the reader through the beginnings of skin tissue engineering,the utilization of some of the key products developed for the treatment of severe burns and the hope of harnessing stem cells to improve on current practice.

关键词： Burns Skin tissue engineering Stem cells Cultured epithelial autografts Dermal substitutes Microskin grafting

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A Review of Skin Banking Guidelines and Standards Worldwide:Towards the Harmonization of Guidelines for Skin Banking in Therapeutic Applications for the Regions under the Asia Pacific Burn Association(APBA)

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Burns & Trauma 2020年第1期8卷 82-93页

作者： Wee Ling Heng Qi Wei Wang Renuka Sornarajah Jacynthe Tremblay Nandita Melati Putri Suzina Sheikh Ab Hamid Pornthep Pungrasmi Hsian-Jenn Wang Dong Chul Kim Daizo Saito Nhu Lam Nguyen Wan Azman Wan Sulaiman Aditya Wardhana Vinita Puri Hajime Matsumura Niann-Tzyy Dai Rajeev BAhuja Gaoxing Luo Weifeng He Si Jack Chong alvin wen choong chua Transplant Tissue Centre Singhealth Duke-NUS Transplant CentreSingapore Heart Valve and Tissue Bank Lady Ridgeway Hospital for ChildrenDr Danister De Silva MawathaColomboSri Lanka Jacynthe Tremblay Consultation QuebecCanada Burn Unit Faculty of MedicineCipto Mangunkusumo General HospitalJakartaIndonesia Otorhinolaryngology-Head and Neck Surgery School of Medical SciencesHealth CampusUniversiti Sains MalaysiaKelantanMalaysia Plastic and Reconstructive Surgery Division King Chulalongkorn Memorial HospitalBangkokThailand Plastic and Reconstructive Surgery Taipei Medical UniversityTaipeiChinese Taipei Plastic and Reconstructive Surgery Bundang Jesaeng HospitalSeongnamSouth Korea Division of Traumatology Research InstituteNational Defence Medical CollegeSaitamaJapan Vietnam National Burn Hospital HanoiVietnam Reconstructive Sciences Programme School of Medical SciencesHealth CampusUniversiti Sains MalaysiaKelantanMalaysia Department of Plastic Surgery King Edward Memorial Hospital and Seth Gordhandas Sunderdas Medical CollegeMumbaiIndia Department of Plastic and Reconstructive Surgery Tokyo Medical UniversityTokyoJapan Division of Plastic and Reconstructive Surgery Department of SurgeryTri-Service General HospitalNational Defense Medical CenterTaipei Plastic and Aesthetic Surgery Sir Ganga Ram HospitalNew DelhiIndia Institute of Burn Research Southwest HospitalArmy Medical UniversityChongqingChina Department of Plastic Reconstructive and Aesthetic SurgerySingapore General HospitalSingapore Musculoskeletal Sciences Academic Clinical Programme Duke-NUS Medical SchoolSingapore

Currently,there are no harmonized guidelines which govern skin banking in the Asia Pacific ***,skin banks are either unregulated or rely on their nation’s legislation or international accreditation to uphold their quality standards.A new set of skin banking guidelines was devel-oped through a comprehensive review and collation of best international practices for the Asia Pacific Burn Association(APBA)members,from donor screening and testing,to skin recovery,processing,storage and distribution,and quality *** regulatory requirements reviewed include the European directives,Australia’s Therapeutic Goods Administration and Sin-gapore’s tissue banking *** technical and quality management recommendations are referenced from the American Association of Tissue Banks(AATB),the United States Food and Drug Administration standards and guidance documents,various relevant European guides,Japanese Society of Tissue Transplantation guidelines and the Asia Pacific Association of Surgical Tissue *** mainly from the AATB standards,the new Asia Pacific Burn Association Guidelines for Skin Banking in Therapeutic Applications offer a comprehensive manual,address-ing:governance and contracts;staff responsibilities;quality management;facilities,equipment and supplies management;donor consent and testing;and recommendations of good practices pertaining to skin recovery,processing,storage and *** complementing current generic regulations,they provide technical specifications of major aspects unaddressed in most *** inaugural set of new regional skin banking guidelines would be a start for regional members of the APBA to adopt,and will hopefully culminate in a set of standards so that,in the long run,skin allografts from this region can be of similar quality,which can simplify import process and facilitate the exchange of allografts between members.

关键词： Skin banking Skin allografts Skin transplantation Tissue banking Tissue donation

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