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Acta Biochimica et Biophysica Sinica 2006年第3期38卷 194-200页

作者： Hai-Jing SUN~(1,2) Xin XU~1 Xiu-Li WANG~1 Liang WEI~1 Fen LI~1 Jun LU~1* Bai-Qu HUANG~11 Institute of Genetics and Cytology,Northeast Normal University,Changchun 130024,China2 Beijing Normal University,Beijing 100875,China Institute of Genetics and Cytology Northeast Normal UniversityChangchun 130024 China Beijing Normal University Beijing 100875 China

There has been considerable interest in researching the regulatory mechanisms that control thesynthesis of interleukin （IL）-12,which plays a central role in the differentiation of T-helper-1 *** thisstudy,we performed a series of transient transfection experiments designed to elucidate the functional rela-tionship between the IL-12 promoter-specific transcription factors （Ets2 and Sp1） and histone acetylationmodification in IL-12 regulation mediated by p300 and various histone deacetylases （HDACs）.Results pre-sented in this report demonstrated that the transcription factors Ets2 and Sp1 acted synergistically with p300to activate the human IL-12 *** histone acetyltransferase （HAT） activity of p300 was required forthis synergic effect,because the adenovirus E1A protein inhibited the ***,HDACs re-pressed the synergic effect of transcription factors and histone acetylation on the activation of IL-12,whilep300 was able to rectify *** data indicated that Ets2 and Sp1 worked concertedly and synergisticallywith p300 in the regulation of human IL-12 expression.

关键词： Ets2 Sp1 histone acetyltransferase p300 interleukin-12

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新一轮家电“以旧换新”行动方案影响

日用电器 2024年第3期 1-2页

作者： 1.华创证券 2.光大证券 3.中信期货华创证券光大证券中信期货

国务院印发《推动大规模设备更新和消费品以旧换新行动方案》（以下简称《行动方案》），明确了5方面20项重点任务和实施设备更新、消费品以旧换新、回收循环利用、标准提升四大行动，提出积极开展家电等消费品以旧换新。《行动方案》...

国务院印发《推动大规模设备更新和消费品以旧换新行动方案》（以下简称《行动方案》），明确了5方面20项重点任务和实施设备更新、消费品以旧换新、回收循环利用、标准提升四大行动，提出积极开展家电等消费品以旧换新。《行动方案》家电直接相关内容1.到2027年废旧家电回收量较2023年增长30%,

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Behavior pattern extraction by trajectory analysis

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Frontiers of Computer Science 2011年第1期5卷 37-44页

作者： Jia Wen (12) wjyanyuan@*** Chao Li (1) Zhang Xiong (1) Beihang Univ Sch Engn & Comp Sci Beijing 100191 Peoples R China Yanshan Univ Dept Comp Coll Informat Sci & Engn Qinhuangdao 066004 Peoples R China

Trajectory clustering and behavior pattern extraction are the foundations of research into activity perception of objects in motion. In this paper, a new framework is proposed to extract behavior patterns through trajectory analysis. Firstly, we introduce directional trimmed mean distance （DTMD）, a novel method used to measure similarity between trajectories. DTMD has the attributes of anti-noise, self-adaptation and the capability to determine the direction for each trajectory. Secondly, we use a hierarchical clustering algorithm to cluster trajectories. We design a length-weighted linkage rule to enhance the accuracy of trajectory clustering and reduce problems associated with incomplete trajectories. Thirdly, the motion model parameters are estimated for each trajectory＇s classification, and behavior patterns for trajectories are extracted. Finally, the difference between normal and abnormal behaviors can be distinguished.

关键词： trajectory clustering directional trimmedmean distance (DTMD) behavior pattern extraction

Ligands of Peroxisome Proliferator-activated Receptor Inhibit Homocysteineinduced DNA Methylation of Inducible Nitric Oxide Synthase Gene

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Acta Biochimica et Biophysica Sinica 2007年第5期39卷 366-376页

作者： Yideng JIANG~(1*), Jianzhong ZHANG~1, Jiantuan XIONG~1, Jun CAO~1, Guizhong LI~1, and Shuren WANG~2 1 Department of Pathology, Ningxia Medical College, Yinchuan 750004. China 2 Department of Pathophysiology, West China College of Preclinical and Forensic Medical Sciences, Sichuan University, Chengdu 610041, China Department of Pathology Ningxia Medical CollegeYinchuan 750004 China Department of Pathophysiology West China College of Preclinical and Forensic Medical Sciences Sichuan UniversityChengdu 610041 China

Homocysteine (Hcy) is a risk factor for atherosclerosis. It is generally accepted that inducible nitric oxide synthase (iNOS) is a key enzyme in the regulation of vascular disease. The aim of the present study is to investigate the effects of peroxisome proliferator-activated receptor ligands on iNOS in the presence of Hcy in human monocytes. Foam cells, induced by oxidize low density lipoprotein (ox-LDL) and phorbol myristate acetate (PMA) in the presence of different concentrations of Hcy, clofibrate and pioglitazone in human monocytes for 4 d, were examined by oil red O staining. The activity of iNOS was detected by real-time quantitative reverse transcription-polymerase chain reaction and Western blot analysis. The capability of DNA methylation was measured by assaying endogenous C5 DNA methyltransferase (C5MTase) activity, and the iNOS promoter methylation level was determined by quantitative MethyLight assays. The results indicated that Hcy increased the activity of C5MTase and the level of iNOS gene DNA methylation, resulting in a decrease of iNOS expression. Clofibrate and pioglitazone could antagonize the Hcy effect on iNOS expression through DNA methylation, resulting in attenuation of iNOS transcription. These findings suggested that Hcy decreased the expression of iNOS by elevating iNOS DNA methylation levels, which can repress the transcription of some genes. Peroxisome proliferator-activated receptor α/γ ligands can down- regulate iNOS DNA methylation, and could be useful for preventing Hcy-induced atherosclerosis by repressing iNOS expression.

关键词： homocysteine DNA methyltransferase PPARα/γ ligand iNOS DNA methylation

RANGE SURVEY OF DEPOSITION IN THE LOWER YELLOW RIVER

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International Journal of Sediment Research 2002年第2期17卷 91-101页

作者： LONG Yuqian1, LIANG Guoting1, ZHANG Yuanfeng1, SHEN Guanqing1, ZHANG Liuzhu1 and CHENG Longyuan1 Yellow River Conservancy Commission Zhengzhou 450003 Henan P.R.China

A range survey database is established and used to study the deposition or erosion processes in the Lower Yellow River (LYR). The huge amount of data obtained through range surveys over nearly 50 years is managed by software (RGTOOLS) developed for this study. RGTOOLS is also used to evaluate the amount and distribution of deposition or erosion in LYR. Volumetric method is used for computation of the amount of deposition. Spatial and temporal variations of the deposition are studied and illustrated with drawings. It is found that a total of 6.44 billion m3 of sediment were deposited in the lower Yellow River over approximately 50 years. The pattern of deposition varies in response to the variation of oncoming flow, which is influenced by natural climatic variations and also by reservoir operation.

关键词： Lower Yellow River Range survey database Erosion and deposition Volumetric method

Molecular mechanism for the substrate recognition of USP7

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Protein & Cell 2015年第11期6卷 849-852页

作者： Jingdong Cheng (1) (2) Ze Li (1) (2) Rui Gong (1) Jian Fang (1) Yi Yang (1) Chang Sun (1) Huirong Yang (1) Yanhui Xu (1) (2) (3) 1.Key Laboratory of Molecular Medicine Ministry of Education Department of Systems Biology for Medicine School of Basic Medical Sciences Shanghai Medical College of Fudan University Shanghai 200032 China 2.School of Basic Medical Sciences Fudan University Shanghai Cancer Center Institutes of Biomedical Sciences Shanghai Medical College of Fudan University Shanghai 200032 China 3.State Key Laboratory of Genetic Engineering School of Life Sciences Collaborative Innovation Center of Genetics and Development Fudan University Shanghai 200433 China

Dear Editor, The ubiquitin specific protease 7 （USP7）, also known as herpes virus associated ubiquitin specific protease （HAUSP）, is a well-characterized deubiquitinase （Reyes- Turcu et al., 2009）. USP7 plays important roles in various biological processes, including cell survival, proliferation, apoptosis, tumorigenesis, viral infection, and epigenetic regulation through regulating the protein stability of tumor suppressors （p53, PTEN, FOXO, claspin）, E3 ligases （MDM2, Mule, viral proteins ICP0）, epigenetic regulators （DNMT1, Tip60, UHRF1）（Pfoh et al., 2015）. USP7 is comprised of three recognizable domains： the N-terminal TRAF domain, the catalytic domain, and the C-terminal Tandem UBL domain （designated TUDusPT）（Fig. 1A）. Previous study shows that the TRAF domain binds to P/AxxS motif （from p53 and MDM2） and is responsible for substrate recognition （Sheng et al., 2006）. However, how USP7 recognizes other substrates remains largely unknown.

关键词： Form Hydrogen Bond Protein ICP0 Latent Viral Infection Human Protein Database Acidic Pocket

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Differentiation profile of brain tumor stem cells:a comparative study with neural stem cells

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Cell Research 2006年第12期16卷 909-915页

作者： Quan Bin Zhang~1 Xiao Yan Ji~1 Qiang Huang~1 Jun Dong~1 Yu De Zhu~1 Qing Lan~1 Neurosurgical Department and Brain Tumor Research Laboratory,Second Affiliated Hospital of Suzhou Universiry,Suzhou 215004,China Neurosurgical Department and Brain Tumor Research Laboratory Second Affiliated Hospital of Suzhou University Suzhou China

Understanding of the differentiation profile of brain tumor stem cells (BTSCs),the key ones among tumor cell popula-tion,through comparison with neural stem cells(NSCs)would lend insight into the origin of glioma and ultimately yieldnew approaches to fight this intractable ***,we cultured and purified BTSCs from surgical glioma specimensand NSCs from human fetal brain tissue,and further analyzed their cellular biological behaviors,especially their differ-entiation *** expected,NSCs differentiated into mature neural phenotypes. In the same differentiation condition,however,BTSCs exhibited distinguished ***,cells grew flattened and attached for the first week,but gradually aggregated and reformed floating tumor sphere *** the corresponding period,the expressionrate of undifferentiated cell marker CD 133 and nestin in BTSCs kept decreasing,but 1 week later,they regained ascend-ing ***,the differentiated cell markers GFAP and β-tubulinⅢ showed an expression change inverse tothat of undifferentiated cell *** together,BTSCs were revealed to possess a capacity to resist differentiation,which actually represents the malignant behaviors ofglioma.

关键词： brain tumor stem cell neural stem cell differentiation

A Preliminary Study of the Noninvasive Remote Control System for Rat Bio-Robot

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Journal of Bionic Engineering 2010年第4期7卷 375-381页

作者： Xitian Pi~(1,2),Shuangshuang Li~1,Lin Xu~1,Hongying Liu~1,Shenshan Zhou~1,Kang Wei~1,Zhenyu Wang~1,Ziru Jia,Xiaolin Zheng~(1,2),Zhiyu Wen~2 1.Bioengineering College,Chongqing University,Chongqing 400030,P.R.China 2.Key Laboratories for National Defense Science and Technology of Innovative Micro-Nano Devices and System Technology,Chongqing University,Chongqing 400030,P.R.China Bioengineering College Chongqing University Chongqing P. R. China Key Laboratories for National Defense Science and Technology of Innovative Micro-Nano Devices and System Technology Chongqing University Chongqing P. R. China

A system is described here that can noninvasively control the navigation of freely behaving rat via ultrasonic,epidermaland LED photic stimulators on the *** system receives commands from a remote host computer to deliver specifiedelectrical stimulations to the hearing,pain and visual senses of the rat *** results demonstrate that the three stimuliwork in groups for the rat *** can control the rat to proceed and make right and left turns with great *** verified that the rat was able to reach a setting destination in the way of cable with the help of a person through theappropriate coordination of the three *** telemetry video camera mounted on the head of the rat also achieveddistant image acquisition and helped to adjust its navigation path over a distance of 300 *** a word,the non-invasive motioncontrol navigation system is a good,stable and reliable bio-robot.

关键词： rat navigation bio-robot ultrasonic stimulator epidermal stimulator LED photic stimulator

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TECA hybrid artificial liver support system in treatment of acute liver failure

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World Journal of Gastroenterology 2001年第6期7卷 826-829页

作者： Yi-Long Xue~1 Shi-Feng Zhao~1 Yun-Luo~1 Xin-Jian Li~1 Zhong-Ping Duan~2 Xiao-Ping Chen~1 Wen-Ge Li~1 Xiao-Qiang Huang~1 Yan-Ling Li~1 Xin-Cui~1 Da-Guang Zhong~1 Zuo-Yun Zhang~1 Zhi-Qiang Huang~1 1 Chinese PLA General Hospital,Beijing 100853,China2 Beijing You-An Hospital,Beijing 100054,China Chinese PLA General Hospital Beijing 100853 China. xueyl@***

AIM: To assess the efficacy and safety of TECA type hybrid artificial liver support system (TECA-HALSS) in providing liver function of detoxification, metabolism and physiology by treating the patients with acute liver failure (ALF). METHODS: The porcine liver cells (1-2) x 10(10) were separated from the Chinese small swine and cultured in the bioreactor of TECA-BALSS at 37.0 degrees C and circulated through the outer space of the hollow fiber tubes in BALSS. The six liver failure patients with various degree of hepatic coma were treated by TECA-HALSS and with conventional medicines. The venous plasma of the patients was separated by a plasma separator and treated by charcoal adsorbent or plasma exchange. The plasma circulated through the inner space of the hollow fiber tubes of BALSS and mixed with the patients' blood cells and flew back to their blood circulation. Some small molecular weight substances were exchanged between the plasma and porcine liver cells. Each treatment lasted 6.0-7.0 h. Physiological and biochemical parameters were measured before,during and after the treatment. RESULTS: The average of porcine liver cells was (1.0-3.0) x 10(10) obtained from each swine liver using our modified enzymatic digestion method. The survival rate of the cells was 85%-93% by trypan blue stain and AO/PI fluorescent stain. After cultured in TECA-BALSS bioreactor for 6 h, the survival rate of cells still remained 70%-85%. At the end of TECA-HALSS treatment, the levels of plasma NH(3), ALT, TB and DB were significantly decreased. The patients who were in the state of drowsiness or coma before the treatment improved their appetite significantly and regained consciousness, some patients resumed light physical work on a short period after the *** to two days after the treatment, the ratio of PTA increased warkedly. During the treatment, the heart rates, blood pressure, respiration condition and serum electrolytes (K(+), Na(+) and Cl(-)) were stable without thrombo

关键词： Liver, Artificial Acute Disease Adult Animals Female Humans Liver Liver Failure Male Middle Aged Research Support, Non-U.S. Gov't Swine Swine, Miniature

Pathway-based analysis for genome-wide association study data of bipolar disorder provides new insights for genetic study

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Protein & Cell 2015年第12期6卷 912-915页

作者： Suhua Chang (1) Jinglu Wang (1) (2) Kunlin Zhang (1) Jing Wang (1) 1.Key Laboratory of Mental Health Institute of Psychology Chinese Academy of Sciences Beijing 100101 China 2.University of Chinese Academy of Sciences Beijing 100049 China

Bipolar disorder （BD） is an episodic recurrent pathological mood disturbance with estimated heritability ranging from 80%-85% （Barnett and Smoller, 2009）. Although many studies have indicated that BD is a polygenic disease influenced by many genes with small effect, the pathogenesis of BD is still not well understood. According to the catalog of published genome-wide association studies （GWAS）（Welter et al., 2014）, there have been 31 BD GWASs （including bipolar disorder related symptoms and combined GWAS with other related disorders）till 01/15/2015, including the largest BD GWAS from the Psychiatric Genomics Consortium （PGC）（Sklar et al., 2011）.

关键词： Bipolar Disorder Retinol GWAS Data Wellcome Trust Case Control Consortium Candidate Pathway

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