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Comparison of Transformation Efficiency of piggyBac Transposon among Three Different Silkworm Bombyx mori Strains

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Acta Biochimica et Biophysica Sinica 2007年第2期39卷 117-122页

作者： Boxiong ZHONG~1 Jianying LI~1 Jin'e CHEN~(1,2) Jian YE~1 and Songdong YU~1 ~1College of Animal Sciences,Zhejiang University,Hangzhou 310029,China ~2Institute of Sericultural Research,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China College of Animal Sciences Zhejiang University Hangzhou 310029 China Institute of Sericultural Research Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China

The transformation rate of three different strains of silkworm Bombyx mori was comparedafter the introduction of enhanced green fluorescence protein （EGFP）-encoding genes into the silkwormeggs by microinjection of a mixture of piggyBac vector and helper plasmid containing a *** there were no significant differences among the three strains in the percentages offertile moths in microinjected eggs （P=0.1258）,the percentages of G0transformed moths in fertile mothsand injected eggs were both significantly different （P=0.01368 and P=0.02398, respectively）.Thetransformation rate of the Nistari strain （Indian strain） was significantly higher than that of the other twostrains,Golden-yellow-cocoon （Vietnamese strain） and Jiaqiu （Chinese strain）,which had similar rate. Theseresults indicate that the transformation efficiency of the piggyBac-based system might vary with silkwormstrains with different genetic *** presence of endogenous piggyBac-like elements might bean important factor influencing the transformation efficiency of introduced piggyBac-derived vectors,andthe diverse amount and activation in different silkworm strains might account for the significant differences.

关键词： Bombyx mori piggyBac transposon transgenesis transformation efficiency

Many-body theories of density response for a strongly correlated Fermi gas

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Frontiers of physics 2012年第1期7卷 98-108页

作者： Hui Hu (1) 1. ARC Centre of Excellence for Quantum-Atom Optics Centre for Atom Optics and Ultrafast Spectroscopy Swinburne University of Technology Melbourne 3122 Australia

Recent breakthroughs in the creation of ultracold atomic gases in the laboratory have ushered in major changes in physical science. Many novel experiments are now possible, with an unprecedented control of interaction, geometry and purity. Quantum many-body theory is facing severe challenges in quantitatively understanding new experimental results. Here, we review some recently developed theoretical techniques that provide successful predictions for density response of a strongly correlated atomic Fermi gas. These include the strong-coupling random-phase approximation theory, high- temperature quantum virial expansion, and asymptotically exact Tan relations applicable at large momentum.

关键词： quantum many-body theory Fermi gas

Experimental determination of interfacial energies for Ag_2Al solid solution in the CuAl_2-Ag_2Al system

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Chinese Physics B 2009年第9期18卷 3952-3959页

作者： Ocak Y Akbulut S Keslioglu K Marasl1 N ad1rl1 E Kaya H Department of Physics Faculty of Arts and SciencesErciyes University Department of Physics Institute of Science and TechnologyErciyes University Department of Physics Faculty of Arts and SciencesNigde University Department of Science Education Education FacultyErciyes University

The equilibrated grain boundary groove shapes of solid solution Ag2Al in equilibrium with an AI-Cu-Ag liquid were observed from a quenched sample with a radial heat flow apparatus. The Gibbs Thomson coefficient, solid liquid interfacial energy and grain boundary energy of the solid solution Ag2Al have been determined from the observed grain boundary groove shapes. The thermal conductivity of the solid phase and the thermal conductivity ratio of the liquid phase to solid phase for Ag2Al-28.3 at the %CuAl2 alloy at the melting temperature have also been measured with a radial heat flow apparatus and Bridgman type growth apparatus, separately.

关键词： solid-liquid interfaces metals and alloys surface energy thermal conductivity

Genetic variation of Ardisia crenata in south China revealed by nuclear microsatellite

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Journal of Systematics and Evolution 2010年第4期48卷 279-285页

作者： 1,2Hong-Ping MU 1Lan HONG 1Hong-Lin CAO 1Zheng-Feng WANG 1Zhong-Chao LI 1 Hao SHEN 1Zhang-Ming WANG 1Wan-Hui YE 1(South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China) 2(Graduate School of the Chinese Academy of Sciences,Beijing 100049,China) (South China Botanical Garden Chinese Academy of Sciences Guangzhou 510650 China) (Graduate School of the Chinese Academy of Sciences Beijing 100049 China)

Ardisia crenata Sims,one of the most widely distributed Ardisia in the world,is an important ornamental and medicinal plant *** seven polymorphic nuclear microsatellite loci,we studied the genetic variation of 20 natural populations of *** across its distribution center in south *** deviation from the Hardy–Weinberg equilibrium in all populations and at all loci were detected,and the fixation index was high (FIS=0.725),indicating that inbreeding may be dominant in the mixed mating system of this self-compatible *** average genetic diversity within populations was relatively low(HS=0.321).There was significant genetic differentiation among populations(FST=0.583),which may have resulted from a high level of inbreeding and a low level of gene *** crenata in south China can be roughly divided into an eastern group and a western group,consistent with the floristic division of the Sino-Himalayan forest subkingdom and the Sino-Japanese forest *** may be separated glacial refugia in each region.

关键词： differentiation genetic variation inbreeding nuclear microsatellite

Copper Elimination from the Molten Steel by Addition of Hydronitrogens

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International Journal of Minerals,Metallurgy and Materials 1998年第2期12卷 93-96页

作者： Liansheng Li~(1)) Changxiang Xiang~(1)) Jie Cao~(1)) Shiqi Ii~(1)) Eiji Ichise~(2)) 1)Applied Science School, UST Beijing, Beijing 100083, China 2) The Kyoto University, Kyoto, Japan Applied Science School UST Beijing Beijing 100083 China Kyoto University Kyoto Japan

A novel method has been proposed to remove copper from molten steel by adding the compounds of hydrogen and nitrogen into the melt at normal pressure. Feasibility experiments were carried out in Mo-wire resistance furnace at 1600℃. The copper content of about 400 g 45 steel were reduced from 0.60% and 0.57% to 0.41% and 0.51% with 3.25g and 1.20 g NH_4Cl respectively, while the copper contents of about 300 g steel melt were reduced from 1.15% and 0.61% to 0.90% and 0.56% with 4.10 g and 2.00 g NH_2CONH_2. These results indicate that the proposed method is very promising to be put into practical and worthwhile of further study.

关键词： molten steel copper removal hydronitrogens

Some Concepts on Statistical Models of Relating Mechanical Properties to Chemical Composition

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International Journal of Minerals,Metallurgy and Materials 1998年第2期12卷 84-86页

作者： Jun Wu~(1)) Chaobin Hao~(2)) Yana Wang~(1)) Shiqi Li~(1)) Linjie Yang~(1)) 1) Metallurgy School, UST Beijing, Beijing 100083, China 2) Chinese Nuclear Information Center Metallurgy School UST Beijing Beijing 100083 China Chinese Nuclear Information Center China

Following the basic principle of modem multivariate statistical analysis theory, the description model, prediction model and control model to relate chemical compositions and mechanical properties of steels are introduced. As an example, the total flowchart of components and structure/properties description, prediction and control model for chemical composition and mechanical properties of 20 and A_2 steel are presented.

关键词： mechanical property statistical model prediction description control

End-Permian conodont fauna from Dongpan section:Correlation between the deep-and shallow-water facies

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Science China Earth Sciences 2008年第11期51卷 1611-1622页

作者： GenMing Luo (1) (2) XuLong Lai (1) (2) QingLai Feng (1) (3) HaiShui Jiang (1) (2) Paul Wignall (4) KeXin Zhang (1) (3) YaDong Sun (2) Jun Wu (1) (2) School of Earth and Environment University of Leeds LS2 9JT Leeds UK

This paper systematically investigated the conodonts from the uppermost Permian to the Lower Triassic at the Dongpan Section, Southern Guangxi, South China, and obtained abundant Late Permian conodonts from the syndepositional limestone lenses of beds 3 and 5-2 at this section. One genus and eight species of conodont P1 element including one new species, Neogondolella dongpanensis sp. nov., have been identified. The feature of conodont fauna indicates that conodonts collected from beds 3 and 5 at the Dongpan Section belong to the Neogondolella yini conodont zone, and correspond to bed 24 at the Meishan Section. Based on these conodont data, we suggest that the Neoalbaillella optima radiolarian zone at the Dongpan Section at least extended to the upper part of the N. yini conodont zone.

关键词： Dongpan Section end-Permian conodont fauna biostratigraphic correlation

Entanglement and nonlocality in multi-particle systems

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Frontiers of physics 2012年第1期7卷 72-85页

作者： Margaret D. Reid (1) Qiong-Yi He (1) Peter D. Drummond (1) 1. ARC Centre of Excellence for Quantum-Atom Optics Centre for Atom Optics and Ultrafast Spectroscopy Swinburne University of Technology Melbourne 3122 Australia

Entanglement, the Einstein-Podolsky Rosen （EPR） paradox and Bell＇s failure of local-hidden- variable （LHV） theories are three historically famous forms of ＂quantum nonlocality＂. We give experimental criteria for these three forms of nonlocality in multi-particle systems, with the aim of better understanding the transition from microscopic to macroscopic nonlocality. We examine the nonlocality of N separated spin J systems. First, we obtain multipartite Bell inequalities that address the correlation between spin values measured at each site, and then we review spin squeezing inequal- ities that address the degree of reduction in the variance of collective spins. The latter have been particularly useful as a tool for investigating entanglement in Bose Einstein eondensates （BEC）. We present solutions for two topical quantum states： multi-qubit Greenberger-Horne Zeilinger （GHZ） states, and the ground state of a two-well BEC.

关键词： entanglement quantum nonlocality multi-particle two-well Bose-Einstein condensates(BEC)

Crystal structure of human Fanconi-associated nuclease 1

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Protein & Cell 2015年第3期6卷 225-228页

作者： Peng-xian Yan(1)(2) Yan-gao Huo (1) Tao Jiang (1) 1.National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China 2.University of Chinese Academy of Sciences Beijing 100039 China

Dear Editor, DNA interstrand crosslinks （ICLs） are toxic to cells because they covalently link the two strands in the duplex. ICLs can be induced by crosslinking agents and by cell endogenous metabolites, and physically prevent transcription and replication in both directions （MacKay et al., 2010）. This type of DNA damage must be properly repaired to maintain genomic and cellular integrity, and this repair is primarily accomplished by the Fanconi anemia pathway （FA pathway）（Moldovan and D＇Andrea, 2009）.

关键词： Fanconi Anemia Electrostatic Surface Potential Fanconi Anemia Pathway Chronic Kidney Failure Shanghai Synchrotron Radiation Facility