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Comparison of Insecticide Resistance and Its Enzyme Mechanisms among Aedes aegypti Collected with Three Methods in a Dengue-Endemic City in southern Mexico

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Advances in Entomology 2022年第3期10卷 252-266页

作者： Walter Eduardo Quezada-Yaguachi Americo D. Rodriguez Francisco solís-santoyo alma delia lópez-solís William C. Black IV Karla saavedra-Rodriguez Diego Morales-Viteri R. Patricia Penilla-Navarro Centro Regional de Investigación en salud Pública Instituto Nacional de Salud Pública Tapachula Mexico Arthropod Borne and Infectious Diseases laboratory Department of Microbiology Immunology and Pathology College of Veterinary Medicine and Biomedical Sciences Colorado State University Fort Collins CO USA sector “El Dorado” Centro de Referencia Nacional de Vectores Instituto Nacional de Investigación en Salud Pública Quito Ecuador

Background: Despite the physical and chemical effort to control Aedes aegypti, the arboviruses transmission in the south of Mexico remains latent. Trying to improve the methods of entomological surveillance routinely used, whether the estimation of resistance to insecticides used for its control, as well as their enzyme mechanisms, were influenced by the phase in which the mosquitoes were collected through three different collection methods was investigated. Materials and Methods: Mosquito collections from the “5 de Febrero” neighborhood in Tapachula, Mexico were obtained by ovitraps, larvitraps, and a CDC backpack aspirator. Insecticide resistance of Fsub>1sub> females was determined by WHO diagnostic doses and resistance ratios (RRsub>50sub>), furthermore, levels of insecticide metabolism enzymes were determined by biochemical assays. Results: Overall, in mosquitoes collected by ovitraps, larvitraps, and CDC backpack aspirator respectively, the low mortalities obtained with the discriminant dose to Malathion (27.57%, 26.97%, and 26.91%), and to Bendiocarb (50.5%, 45.36%, and 54.97%) suggest resistance. However, lCsub>50sub> for Malathion (0.922, 0.934, and 0.915) and for Bendiocarb (0.112, 0.109, and 0.107);and the low resistance ratios (RRsub>50sub>) for Malathion (3.34, 3.29, and 3.27) and for Bendiocarb (2.15, 2.1, and 2.06) does not suggest resistance. Although a slight numerical variation is observed between the three lCsub>50sub> values, the overlap observed between their confidence intervals allows us to assume that there were no differences between the three methods. In general, esterases (determined with three substrates), glutathion s-transferases (GsT) and cytochromes Psup>450sup> were statistically higher than those of the susceptible strain;and the three enzyme levels were statistically different among the three collection methods (P Conclusion: Although using a CDC backpack aspirator demonstrated being the best collection method det

关键词： CDC Backpack Aspirator Insecticide Resistance larvitraps Mosquitoes Ovitraps

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Neutralization of Omicron BA.1,BA.5.1.6,BQ.1.3 and XBB1.1 induced by heterologous vaccination Ad5-nCoV and mRNA-1273

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signal Transduction and Targeted Therapy 2023年第5期8卷 2085-2087页

作者： Jesús Hernández Freddy Dehesa-Canseco alma B.Vázquez-lópez Mónica Reséndiz-sandoval Graciela Caire-Juvera Mario solís-Hernández Olivia Valenzuela Bruno Gómez-Gil Verónica Mata-Haro Centro de Investigación en Alimentación y Desarrollo A.C.HermosilloSonora83304Mexico Comisión México-Estados Unidos para la Prevención de la Fiebre Aftosa y otras Enfermedades Exóticas de los Animales(CPA) SENASICASADERCiudad de México05010Mexico Departamento de Ciencias Químico Biológicas Universidad de SonoraHermosilloSonora83000Mexico

Dear Editor,The emergence of Omicron subvariants has required additional boosters to induce a highly neutralizing antibody response.1 However,the new Omicron subvariants BQ.1 and XBB,evade the humoral response induced by multiple mRNA vaccinations and infections.2,3 CansinoBio(Ad5-nCoV)is a single-dose adenovirus vector-based vaccine with high protection against severe disease4 but lower efficacy and antibody response than mRNA ***,hybrid immunity induced by infection plus vaccine or heterologous vaccines has produced a robust neutralizing antibody response.

关键词： vaccination vaccine immunity

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sustainability of microporous polymers and their applications

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science China Chemistry 2017年第8期60卷 1033-1055页

作者： Tamara l.Church alma Berenice Jasso-salcedo Fredrik Bjrnerbck Niklas Hedin Department of Materials and Environmental Chemistry Stockholm University SE-106 91 Stockholm Sweden

Microporous polymers(MPs) are studied for their intriguing chemistry and physics as well as their potential application in catalytic transformations, gas-separation processes, water purification and so on. Here, we critically review MPs with respect to the sustainability aspects of their synthesis as well as their applications that have sustainable character. some MPs have been synthesized from monomers derived from biomass resources, but there is certainly a large potential for further *** are also opportunities to improve the sustainability of MP synthesis in terms of the use of solvents, catalysts, and related aspects. The applications of MPs in processes related to sustainability depend upon multiple properties. A rich and flexible chemistry is important to applications as catalysts for, among other useful reactions, the photoreduction of CO_2 and selective oxidation. The(ultra)micropore volume of MPs are crucial in gas-separation applications such as CO_2 capture, and the chemisorption of CO_2 on MP-tethered alkylamines could offer a means to remove that gas from dilute mixtures. When it comes to the storage of H_2 and CH_4 in MPs for onboard use in fuel cell or biogas cars, volumetric capacity is paramount, meaning that the density of the MPs must be considered. Finally, for use in separation and purifications from liquid mixtures(aqueous or hydrocarbon-based), crosslinked MPs are more limited than the solution-processable MPs that can be more easily processed into films and membranes.

关键词： microporous polymers biomass sustainability CO2 capture catalysis H2 storage CH4 storage separation purification

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HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium

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World Journal of Gastroenterology 2022年第29期28卷 3886-3902页

作者： Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio alma l Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo sandra Mendoza-Elizalde Edgar O lópez-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-lópez Marcela salazar García Norma Velázquez-Guadarrama laboratorio de Investigación en Enfermedades Infecciosas Hospital Infantil de México Federico GómezMexico City 06720Mexico Posgrado en Biomedicina y Biotecnología Molecular Laboratorio de Producción y Control de BiológicosEscuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalMexico City 11340Mexico laboratorio de Adquisición de Hierro Universidad Autónoma de la Ciudad MéxicoPosgrado Ciencias GenómicaMexico City 03100Mexico laboratorio Central de Microscopía Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalMexico City 11340Mexico Centro de estudios económicos y sociales en salud Hospital Infantil de México Federico GómezMexico City 06720Mexico laboratorio de Investigación en Biología del Desarrollo y Teratogénesis Experimental Hospital Infantil de México Federico GómezMexico City 06720Mexico

BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence *** To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGs) *** Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGs cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPAsy platform, and using the GlYCOPP platform for glycosylation sites. Different programs, via, I-TAssER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional *** HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium *** technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGs *** analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein

关键词： Hypothetical protein HP0953 Adherence Helicobacter pylori Glycocalyx Virulence factor Persistence

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Authors and Open Access:Effective Ways to Achieve OA in China

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图书情报工作 2005年第12期49卷 17-25页

作者： alma swan Key Perspectives ltd. 48 Old Coach Road Playing PlaceTruroTR3 6ETUnited Kingdom

Although there are effective methods available to authors for providing open access to their work, more than half are still not doing so and provision in China is poorer than in many other countries. There are a number of issues and concerns that dissuade authors from making their work open access: some are still unaware of the concept and of the increased visibility and impact that open access brings; many are unfamiliar with open access journals and how they work; many are uninformed about self-archiving and for some of those who are aware of the possibility of providing open access by this means, concerns about copyright and technical issues *** all these worries can be addressed with simple facts that reassure and encourage authors to adopt open access to benefit themselves,their research and their teaching. There is also a wealth of resources now available to authors that provide information and advice on open access and its effects. As institutions and research funders, both with a strong interest in maximising the visibility and impact of research they support, begin to develop formal policies on open access, models for its provision are emerging. The optimal model is a frills' variant) or by service providers who add functionality or selectivity to provide users with value-enhanced products.

关键词：中国 OA 信息资源网格技术学术材料资源共享

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Experimental hybridization and reproductive isolation between two sympatric species of tephritid fruit flies in the Anastrepha fraterculus species group

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Insect science 2018年第6期25卷 1045-1055页

作者： Juan Rull Eduardo Tadeo Rodrigo lasa Christian l.Rodriguez alma Altuzar-Molina Martin Aluja PROIMI Biotecnologia-OONICET LIEMEN-Division Control Biologico de PlagasTucumanArgentina Red de Manejo Biorracional de Plagas y Vectores Instituto de EcologiaA.C.XalapaVeracruzMexico

Among tephritid fruit flies,hybridization has been found to produce local adaptation and speciation,and in the case of pest species,induce behavioral and ecological alterations that can adversely impact efficient pest *** species group within Anastrepha (Diptera:Tephritidae),is a rapidly radiating aggregate,which includes cryptic species complexes,numerous sister species,and several pest species. Molecular studies have highlighted the possibility of introgression between *** and *** isolation has been studied among morphotypes of the A. fraterculus species complex as a tool for species *** we examined the exis- tence and strength of prezygotic and postzygotic isolation between sympatric populations of two closely related species within the highly derivedfraterculus group (*** and ***),coexisting in *** adults of both species showed a strong tendency for assortative mating,a small proportion of hybrid pairings in both directions were *** also observed asymmetric postzygotic isolation,with one hybrid cross displaying a strong reduction in fecundity and F1 egg *** was greater for the progeny of homotypic and hybrid crosses in the maternal *** was a marked female biased sex ratio distortion for both F1 hybrid *** between *** and *** in nature may be difficult but possible;these two species display stronger reproductive isolation than all pairs of species previously examined in the *** species *** postzygotie isolation is suggestive of Wolbachia mediated cytoplasmic incompatibilities that may be exploited in area-wide pest management.

关键词： Anastrephafraterculus Anastrepha obliqua Haldane's rule introgression prezygotic isolation postzygotic isolation

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Chemical Treatment to Recover Molybdenum and Vanadium from spent Heavy Gasoil Hydrodesulfurization Catalyst

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Advances in Chemical Engineering and science 2012年第3期2卷 408-412页

作者： alma delia Rojas-Rodríguez Orlando Flores-Fajardo Fabiola selene Alcántar González Néstor Noé lópez Castillo Modesto Javier Cruz Gómez Chemical Engineering Department of the Faculty of Chemistry Universidad Nacional Autónoma de México (UNAM) Coyoacán México Faculty of Engineering Universidad Anáhuac México Norte México City México

large quantities of spent hydrodesulfurization (HDs) catalysts are available from petrochemical industry. Disposal of spent catalyst is a problem as it falls under the category of hazardous industrial waste due to its vanadium concentration. Most of these catalysts are usually supported on alumina containing a variable percentage of elements such as nickel or molybdenum. Hence these catalysts contain environmentally critical, and economically valuable metals such as molyb denum, vanadium, and, nickel. In this paper, a spent HDs catalyst was treated with caustic soda solution. Parameters such as temperature, time, and NaOH solution concentration have been studied thoroughly, in order to settle the appropriate conditions for the maximum recovery of molybdenum and vanadium. Under the best leaching conditions (20 %w NaOH, room temperature, 2 h) about 95% recovery of Mo and V was achieved, and the recovery of nickel obtained was of 99% in the form of NiAlO4.

关键词： spent HDs Catalyst Vanadium Molybdenum Metal Recovery

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Biogenic production of seleno-amino acids and seleno-nanoparticles by saccharomyces boulardii

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Food Bioscience 2023年第3期53卷 840-847页

作者： lourdes González-salitre Araceli Castañeda-Ovando Ulin Antobelli Basilio-Cortés Adelfa del Carmen García-Contreras Gabriela Mariana Rodríguez serrano Alejandra Cardelle-Cobas alma delia Román-Gutiérrez luis Guillermo González-Olivares Universidad Autónoma del Estado de Hidalgo Área Académica de QuímicaCiudad del ConocimientoMineral de la ReformaHidalgoMexico Área Académica de Biotecnología Agropecuaria Instituto de Ciencias AgrícolasUniversidad Autónoma de Baja CaliforniaMexico Departamento de Biotecnología División de Ciencias Biológicas y de la SaludUnidad IztapalapaUniversidad Autónoma MetropolitanaCiudad de MéxicoMexico Departamento de Producción Agrícola y Animal Unidad XochimilcoUniversidad Autónoma MetropolitanaCiudad de MéxicoMexico laboratorio de Higiene Inspección y Control de AlimentosDepartamento de Química AnalíticaNutrición y BromatologíaUniversidade de Santiago de CompostelaLugoSpain

selenium is an essential element for humans as it involves different metabolic *** bioavailable and bioaccessible selenium species are produced by microorganisms bioconverting inorganic selenium into nanoparticles and seleno-amino acids,which are of technological and health ***,the objective of this study was the biogenic production of seleno-amino acids and selenium nanoparticles by saccharomyces *** was found that the minimum inhibitory concentration of selenite that allowed the viability of the yeast,the biotransformation of selenium,and the production of selenium nanoparticles was 74 ppm of sodium selenite in YPD *** yeast accumulated 3.4 mg/g of selenium after 9 h of fermentation in the YPD broth enriched with the calculated minimum inhibitory concentration of Na_(2)seO_(3).The presence of selenomethionine,selenocysteine,and selenium nanoparticles was verified through RP-HPlC and TEM *** results demonstrated that the biogenic production of seleno-amino acids and seleno-nanoparticles by the probiotic yeast *** is *** compounds could have applications in the fields of food technology and medicine.

关键词： selenium seleno-amino acid seleno-nanoparticle Probiotic yeast

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A Brassinosteroid-signaling Kinase Interacts withMultiple Receptor-like Kinases in Arabidopsis

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Molecular Plant 2014年第2期7卷 441-444页

作者： Peng Xu shouling Xu ZiJing li Wenqiang Tang alma l Burlingame ZhiYong Wang Institute of Molecular and Cell Biology Hebei Key Laboratory of Molecular and Cellular Biology College of Life Sciences Hebei Normal University Shijiazhuang Hebei 050024 China.

Dear Editor, Higher plants have evolved hundreds of genes encodingreceptor-like kinases （RlKs）, which function as cell surfacereceptors perceiving developmental and environmental sig-nals （shiu et al., 2004）. Many RlKs have been shown to playspecific roles in hormone responses, developmental regula-tion, defense against pathogen infection, and adaptationto abiotic stresses （Chae et al., 2009; Antolin-llovera et al.,2012）. The mechanisms that ensure specific signal transduc-tion from each RlK to target cellular responses remain poorlyunderstood. Recent studies revealed that many RlKs trans-duce signals by phosphorylating receptor-like cytoplasmickinases （RlCKs）, which lack the transmembrane domainsbut are anchored at the plasma membrane through lipidmodification （Tang et al., 2008; Zhang et al., 2010; shi et al.,2013）. There are over 400 RlKs and only about 150 RlCKs inArabidopsis （shiu et al., 2004）. One outstanding question iswhether each RlCK mediates signaling downstream of a spe-cific RlK, participates in multiple RlK pathways, or mediatescrosstalk between RlK pathways.

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The effect of fasting and body reserves on cola tolerance in 2 pit-building insect predators

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Current Zoology 2017年第3期63卷 287-294页

作者： Inon sCHARF alma DANIEl Heath Andrew MACMIllAN Noa KATZ Department of Zoology Faculty of Life Sciences Tel Aviv University POB 39040 69978 Tel Aviv Israel Department of Biology York University 4700 Keele St. Toronto Ontario Canada

Pit-building antlions and wormlions are 2 distantly-related insect species, whose larvae construct pits in loose soil to trap small arthropod prey. This convergent evolution of natural histories has led to additional similarities in their natural history and ecology, and thus, these 2 species encounter similar abiotic stress （such as periodic starvation） in their natural habitat. Here, we measured the cold tolerance of the 2 species and examined whether recent feeding or food deprivation, as well as body composition （body mass and lipid content） and condition （quantified as mass-to-size residuals） affect their cold tolerance. In contrast to other insects, in which food deprivation either enhanced or impaired cold tolerance, prolonged fasting had no effect on the cold tolerance of either species, which had similar cold tolerance. The 2 species differed, however, in how cold tolerance related to body mass and lipid content： although body mass was positively correlated with the wormlion cold tolerance, lipid content was a more reliable predictor of cold tolerance in the antlions. Cold tolerance also underwent greater change with ontogeny in wormlions than in antlions. We discuss possible reasons for this lack of effect of food deprivation on both species＇ cold tolerance, such as their high starvation tolerance （being sit-and-wait predators）.

关键词： chill coma deprivation ecophysiology fat content food hunger morphometrics Myrmeleontidae Vermileonidae.

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