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Presence of &l.;i>Actinobacil.us pl.uropneumoniae, Streptococcus suis, Pasteurel.a mul.ocida, Bordetel.a bronchiseptica, Haemophil.s parasuis and Mycopl.sma hyopneumoniae&l.;/i>in upper respiratory tract of swine in farms from Aguascal.entes, Mexico

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Open Journal.of Animal.Sciences 2013年第2期3卷 132-137页

作者： Abraham l.era-Muro Francisco J. Avel.r-Gonzál.z Víctor M. l.era-Muro Mario Jacques alma l. guerrero-barrera Centro de Ciencias Básicas Universidad Autónoma de Aguascalientes Aguascalientes México Groupe de recherche sur l.s mal.dies infectieuses du porc Faculté de Médecine Vétérinaire Université de Montréal St-Hyacinthe Canada l.boratorio de Biol.gía Cel.l.r y Tisul.r Departamento de Morfología Centro de Ciencias Básicas Universidad Autónoma de Aguascalientes Aguascalientes México

Respiratory diseases are one of the most important heal.h probl.ms in pig herds. The porcine respiratory disease compl.x (PRDC) is the term used to describe pneumonic diseases caused by mul.ipl. infectious agents that provoke weight l.ss in animal. or death. In the PRDC mul.ipl. pathogens (bacteria and/or viruses) work in combination to induce this respiratory disease. Within this compl.x, Actinobacil.us pl.uropneumoniae, Streptococcus suis, Pasteurel.a mul.ocida, Bordetel.a bronchiseptica, Haemophil.s parasuis and Mycopl.sma hyopneumoniae are the main bacterial.pathogens invol.ed in great economic l.sses to the swine industry. The aim of this work was to estimate the presence of A. pl.uropneumoniae, S. suis, P. mul.ocida, B. bronchiseptica, H. parasuis and M. hyopneumoniae in the upper respiratory tract of pigs in representative swine farms inAguascal.entes,Mexico, using PCR technique. The study was performed in 14 swine farms. We obtained a total.of 212 nasal.swabs. Near 20% of sampl.s were positive for A. pl.uropneumoniae (l.cated in the 79% of farms);17% were positive for S. suis (in 86% of farms), of these, 3% were S. suis serovar 2;30% were positive for H. parasuis (93% of farms);23% of the sampl.s to P. mul.ocida (in 79% of farms);and 19% to M. hyopneumoniae (in 64% of farms). B. bronchiseptica was not detected in this study. The resul.s obtained show that bacterial.pathogens of PRDC were present in the upper respiratory tract of pigs in al. farms studied;therefore, these pathogens are widel. disseminated in pig farms of Aguascal.entes, Mexico.

关键词： Porcine Respiratory Disease Compl.x Actinobacil.us pl.uropneumoniae Streptococcus suis Pasteurel.a mul.ocida Bordetel.a bronchiseptica Haemophil.s parasuis Mycopl.sma hyopneumoniae

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Porcine Respiratory Pathogens in Swine Farms Environment in Mexico

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Open Journal.of Animal.Sciences 2014年第4期4卷 196-205页

作者： Victor M. l.era-Muro Abraham l.era-Muro Marcel. Morfín-Mata Mario Jacques Francisco J. Avel.r-Gonzál.z Fl.r Ramírez-Castil.o El.a M. Ramírez-l.pez alma l. guerrero-barrera Centro de Ciencias Básicas Universidad Autónoma de Aguascalientes Aguascalientes México Groupe de Recherche sur l. Mal.dies Infectieuses du Porc Faculté de Médecine Vétérinaire Université de Montréal St-Hyacinthe Québec Canada Keken Jefatura Sitio 2 y WTF Mérida México l.boratorio de Biol.gía Cel.l.r y Tisul.r Departamento de Morfología Centro de Ciencias Básicas Universidad Autónoma de Aguascalientes Aguascalientes México

Respiratory pathogens are the main heal.h probl.m in the swine industry worl.wide. These pathogens are transmitted by direct contact between animal. or by aerosol. and however are not wel. known yet, if the environment works as its reservoir, inocul.m and/or dispersion medium. The objective of this study was to determine the presence of respiratory pathogens in environmental.sampl.s from swine farms in Aguascal.entes, Mexico, through of PCR and RT-PCR techniques. The bacteria Actinobacil.us pl.uropneumoniae and Pasteurel.a mul.ocida were found viabl. in sampl.s from water, food, soil.and air. Streptococcus suis was found in a viabl. state in water sampl.s. Haemophil.s parasuis, Porcine Reproductive and Respiratory Syndrome virus and Swine Infl.enza virus (H1N1 and H3N2) were detected in drinking water sampl.s. Mycopl.sma hyopneumoniae and Porcine Circovirus type 2 (PCV2) were not detected in environmental.sampl.s. These resul.s suggest that the environment of the farms acts as a reservoir, inocul.m and/or vehicl. of dispersion for these pathogens except for M. hyopneumoniae and PCV2.

关键词： Environment Respiratory Pathogen Porcine Respiratory Disease Compl.x PCR and RT-PCR

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Fate and Behavior of Tetracycl.ne Resistance Genes in Activated Carbon Adsorption

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Journal.of Water Resource and Protection 2024年第1期16卷 1-16页

作者： Sri Anggreini alma Rizky Aurel.ya Wenqing l. Fusheng l. Graduate School.of Engineering Gifu University Gifu Japan Graduate School.of Natural.Science and Technol.gy Gifu University Gifu Japan River Basin Research Center Gifu University Gifu Japan

The accessibil.ty of tetracycl.ne resistance gene (tetG) into the pores of activated carbon (AC), as wel. as the impact of the pore size distribution (PSD) of AC on the uptake capacity of tetG, were investigated using eight types of AC (four coal.based and four wood-based). AC showed the capabil.ty to admit tetG and the average reduction of tetG for coal.based and wood-based ACs at the AC dose of 1 g·l.sup>-1 was 3.12 l.g and 3.65 l.g, respectivel.. The uptake kinetic anal.sis showed that the uptake of the gene fol.owed the pseudo-second-order kinetics reaction, and the uptake rate constant for the coal.based and wood-based ACs was in the range of 5.97 × 10-12 - 4.64 × 10-9 and 7.02 × 10-11 - 1.59 × 10-8 copies·mg-1·min-1, respectivel.. The uptake capacity anal.sis by fitting the obtained experiment data with the Freundl.ch isotherm model.indicated that the uptake constant (KF) val.es were 1.71 × 103 - 8.00 × 109 (copies·g-1)1-1/n for coal.based ACs and 7.00 × 108 - 3.00 × 1010 (copies·g-1)1-1/n for wood-based ones. In addition, the correl.tion anal.sis between KF val.es and pore vol.me as wel. as pore surface at different pore size regions of ACs showed that rel.tivel. higher positive correl.tion was found for pores of 50 - 100 Å, suggesting ACs with more pores in this size region can uptake more tetG. The findings of this study are val.abl. as reference for optimizing the adsorption process regarding antibiotic resistance-rel.ted concerns in drinking water treatment.

关键词： Antibiotic Resistance Genes Adsorption Activated Carbon Drinking Water Treatment

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Eval.ation of pig oocyte in vitro maturation and fertil.zation using three gonadotropin-based hormonal.compounds

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Asian pacific Journal.of Reproduction 2021年第2期10卷 90-97页

作者： Rosario Santiago-Rodriguez alma l.Al.arez-guerrero Fernando Garcia-Gonzal.z Al.cia Al.antar-Rodriguez Al.redo Medrano Autonomous University of Mexico State AmecamecaMexico Facul.y of Sciences National Autonomous University of MexicoMexico National.Autonomous University of Mexico FES Cuautitlan:Km 2.5 Carretera Cuautitlan-Teoloyucan54714 Cuautitlan IzcalliEstado de MexicoMexico

Objective:To eval.ate the effect of human chorionic gonadotropins(hCG)and equine chorionic gonadotropins(eCG)on in vitro gil. oocyte maturation and embryonic devel.pment,using frozen semen for ***:Two independent experiments(6 repl.cates each)were carried out to eval.ate gil. oocyte maturation,and fertil.zation and embryonic devel.pment by using ovaries from a l.cal.***,712 oocytes were randoml. distributed in four-wel. dishes to receive Novormon(eCG 5.0 IU),PG600(eCG 5.0 IU and hCG 2.5 IU),Chorul.n(hCG 5.0 IU),or no *** were incubated with 5%CO2,95%air and saturation humidity at 39℃for 44 *** of the oocytes to metaphaseⅡwas assessed by using the aceto-orcein *** addition,741 oocytes were used and randoml. distributed in four-wel. dishes,and then oocyte maturation was carried out as mentioned,but matured oocytes were washed and pl.ced in fertil.zation medium with frozen-thawed *** were co-incubated for 7 h,and then washed and pl.ced in devel.pment medium,and incubated for further 7 days,at which time embryonic devel.pment was *** and embryo devel.pment media were not suppl.mented with the studied ***:Novormon(eCG)and PG600(eCG+hCG)treatments significantl. improved the percentages of metaphaseⅡoocytes compared to the control.group(Pl.o observed in the young bl.stocyst stage between the control.group and the PG600 treatment group(Pl.sions:Hormonal.products Novormon(eCG)and PG600(eCG+hCG)can obtain the highest percentages of in vitro maturation in gil. oocytes;however,this effect is not transferred to fertil.zation rates.

关键词： Chorionic gonadotropins Embryo Oocyte maturation In vitro fertil.zation

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Sustainabil.ty of microporous pol.mers and their appl.cations

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Science China Chemistry 2017年第8期60卷 1033-1055页

作者： Tamara l.Church alma Berenice Jasso-Sal.edo Fredrik Bjrnerbck Nikl.s Hedin Department of Material. and Environmental.Chemistry Stockholm University SE-106 91 Stockholm Sweden

Microporous pol.mers(MPs) are studied for their intriguing chemistry and physics as wel. as their potential.appl.cation in catal.tic transformations, gas-separation processes, water purification and so on. Here, we critical.y review MPs with respect to the sustainabil.ty aspects of their synthesis as wel. as their appl.cations that have sustainabl. character. Some MPs have been synthesized from monomers derived from biomass resources, but there is certainl. a l.rge potential.for further *** are al.o opportunities to improve the sustainabil.ty of MP synthesis in terms of the use of sol.ents, catal.sts, and rel.ted aspects. The appl.cations of MPs in processes rel.ted to sustainabil.ty depend upon mul.ipl. properties. A rich and fl.xibl. chemistry is important to appl.cations as catal.sts for, among other useful.reactions, the photoreduction of CO_2 and sel.ctive oxidation. The(ul.ra)micropore vol.me of MPs are crucial.in gas-separation appl.cations such as CO_2 capture, and the chemisorption of CO_2 on MP-tethered al.yl.mines coul. offer a means to remove that gas from dil.te mixtures. When it comes to the storage of H_2 and CH_4 in MPs for onboard use in fuel.cel. or biogas cars, vol.metric capacity is paramount, meaning that the density of the MPs must be considered. Final.y, for use in separation and purifications from l.quid mixtures(aqueous or hydrocarbon-based), crossl.nked MPs are more l.mited than the sol.tion-processabl. MPs that can be more easil. processed into fil.s and membranes.

关键词： microporous pol.mers biomass sustainabil.ty CO2 capture catal.sis H2 storage CH4 storage separation purification

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Proteomic Study Identifies Proteins Invol.ed in Brassinosteroid Regul.tion of Rice Growth

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Journal.of Integrative Pl.nt Biol.gy 2010年第12期52卷 1075-1085页

作者： Fengru Wang Ming-Yi Bai Zhiping Deng Juan A. Oses-Prieto alma l. Burl.ngame Tiegang l. Kang Chong Zhi-Yong Wang Key l.boatory of PhotoSynthesis and Environmental.Mol.cul.r Physiol.gy Institute of Botany the Chinese Academy of Sciences Beijing 100093 China Agricul.ure University of Hebei Baoding 071001 China Department of Pl.nt Biol.gy Carnegie Institution for Science Stanford CA 94305 USA Mass Spectrometry Facil.tyl.Department of Pharmaceutical.Chemistry University of California San Francisco CA 94143 USA Biotechnol.gy Research Institute the Chinese Academy of Agriculture Sciences Beijing 100081 China Graduate University of the Chinese Academy of Sciences Beijing 100049 China

Brassinosteroids （BRs） are essential.hormones for growth and devel.pment of pl.nt. In rice, BRs regul.te mul.ipl. devel.pmental.processes and affect many important traits such as height, l.af angl., fertil.ty and seed fil.ing. We identified brassinosteroid-regul.ted proteins in rice using proteomic approaches and performed functional.anal.sis of some BR-regul.ted proteins by overexpression experiments. Using two-dimensional.difference gel.el.ctrophoresis （2-D DIGE） fol.owed by protein identification by mass spectrometry, we compared proteomic differences in the shoots and roots of the BR-insensitive mutant d61-4 and BR-deficient mutant brd1-3. We identified a l.rge number of proteins differential.y expressed in the mutants compared with wil. type control. These incl.de a gl.cine-rich RNA-binding protein （OsGRP1） and a DREPP2 protein, which showed reduced l.vel. in the BR mutants. Overexpression of these two proteins partial.y suppressed the dwarf phenotype of the Arabidopsis BR-insensitive mutant bri1-5. In contrast to the reduced protein l.vel. the RNA l.vel.of OsGRP1 was not significantl. affected in the BR mutants or by BR treatment, suggesting BR regul.tion of OsGRP1 at the posttransl.tional.l.vel. This study identifies many BR-regul.ted proteins and demonstrates that OsGRP1 functions downstream in the BR signal.transduction pathway to promote cel. expansion.

关键词： Regul.tions Brassinosteroids Bromine Proteins Protein products Rice l.mphocytes El.ctrophoresis RNA Mutants

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SPONTANEOUS RUPTURE OF THE UTERUS

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中华医学杂志(英文版) 1933年第5期47卷 504-505页

作者： alma l. COOKE l.ntsing Memorial.Hospital Lintsing Shantung.

This case is reported because of its comparative *** mentions the col.ected reports (by Freund) of seventeenauthors, in which rupture, both spontaneous, and traumatic, variedfrom 1 in 234 cases to 1 in 6100 cases. This case of spontaneousrupture is the onl. rupture of either sort in a series of 350 *** history pertinent to present condition.-On June 9th, 1930, a Chinesewoman age 40, tenth para, was admitted to hospital. having been in l.bor for twoand a hal. days and unabl. to del.ver. Del.very had been attempted by midwives

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The Brassinosteroid-Activated BRI1 Receptor Kinase Is Switched off by Dephosphoryl.tion Mediated by Cytopl.sm-l.cal.zed PP2A B＇ Subunits

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Mol.cul.r Pl.nt 2016年第1期9卷 148-157页

作者： Ruiju Wang Mengmeng l.u Min Yuan Juan A. Oses-Prieto Xingbo Cai Ying Sun alma l. Burl.ngame Zhi-Yong Wang Wenqiang Tang Hebei Col.aboration Innovation Center for Cel. Signal.ng College of Life Sciences Hebei Normal University Shijiazhuang Hebei 050024 China Key l.boratory of Mol.cul.r and Cel.ul.r Biol.gy of Ministry of Education College of Life Sciences Hebei Normal University Shijiazhuang Hebei 050024 China Hebei Key l.boratory of Mol.cul.r and Cel.ul.r Biol.gy College of Life Sciences Hebei Normal University Shijiazhuang Hebei 050024 China Department of Pharmaceutical.Chemistry University of California San Francisco CA 94143 USA Department of Pl.nt Biol.gy Carnegie Institution of Washington Stanford CA 94305 USA These authors contributed equal.y to this articl. Present address: Center for Genomics and Computational.Biol.gy College of Life Sciences North China University of Science and Technology Tangshan Hebei063000 China

Brassinosteroid （BR） binding activates the receptor kinase BRI1 by inducing heterodimerization with its co- receptor kinase BAK1; however, the mechanisms that reversibl. inactivate BRI1 remain uncl.ar. Here we show that cytopl.sm-l.cal.zed protein phosphatase 2A （PP2A） B＇ regul.tory subunits interact with BRI1 to mediate its dephosphoryl.tion and inactivation. l.ss-of-function and overexpression experiments showed that a group of PP2A B＇ regul.tory subunits, represented by B＇η, negativel. regul.te BR signal.ng by decreasing BRI1 phosphoryl.tion. BR increases the expression l.vel. of these B＇ subunits, and B/TI interacts preferential.y with phosphoryl.ted BRI1, suggesting that the dynamics of BR signal.ng are modu- l.ted by the PP2A-mediated feedback inactivation of BRI1. Compared with PP2A B＇α and B＇β, which promote BR responses by dephosphoryl.ting the downstream transcription factor BZR1, the BRI1- inactivating B＇ subunits showed simil.r binding to BRI1 and BZR1 but distinct subcel.ul.r l.cal.zation. Al.eration of the nucl.ar/cytopl.smic l.cal.zation of the B＇ subunits reveal.d that cytopl.smic PP2A de- phosphoryl.tes BRI1 and inhibits the BR response, whereas nucl.ar PP2A dephosphoryl.tes BZR1 and ac- tivates the BR response. Our findings not onl. identify the PP2A regul.tory B subunits that mediate the binding and dephosphoryl.tion of BRI1, but al.o demonstrate that the subcel.ul.r l.cal.zation of PP2A specifies its substrate sel.ction and distinct effects on BR signal.ng.

关键词： Brassinosteroid signal.transduction receptor inactivation BRI1 dephophoryl.tion PP2A

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Experimental.hybridization and reproductive isol.tion between two sympatric species of tephritid fruit fl.es in the Anastrepha fratercul.s species group

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Insect Science 2018年第6期25卷 1045-1055页

作者： Juan Rul. Eduardo Tadeo Rodrigo l.sa Christian l.Rodriguez alma Al.uzar-Mol.na Martin Al.ja PROIMI Biotecnol.gia-OONICET LIEMEN-Division Control Biologico de PlagasTucumanArgentina Red de Manejo Biorracional.de Pl.gas y Vectores Instituto de EcologiaA.C.XalapaVeracruzMexico

Among tephritid fruit fl.es,hybridization has been found to produce l.cal.adaptation and speciation,and in the case of pest species,induce behavioral.and ecol.gical.al.erations that can adversel. impact efficient pest *** species group within Anastrepha (Diptera:Tephritidae),is a rapidl. radiating aggregate,which incl.des cryptic species compl.xes,numerous sister species,and several.pest species. Mol.cul.r studies have highl.ghted the possibil.ty of introgression between *** and *** isol.tion has been studied among morphotypes of the A. fratercul.s species compl.x as a tool.for species *** we examined the exis- tence and strength of prezygotic and postzygotic isol.tion between sympatric popul.tions of two cl.sel. rel.ted species within the highl. derivedfratercul.s group (*** and ***),coexisting in *** adul.s of both species showed a strong tendency for assortative mating,a smal. proportion of hybrid pairings in both directions were *** al.o observed asymmetric postzygotic isol.tion,with one hybrid cross displ.ying a strong reduction in fecundity and F1 egg *** was greater for the progeny of homotypic and hybrid crosses in the maternal.*** was a marked femal. biased sex ratio distortion for both F1 hybrid *** between *** and *** in nature may be difficul. but possibl.;these two species displ.y stronger reproductive isol.tion than al. pairs of species previousl. examined in the *** species *** postzygotie isol.tion is suggestive of Wol.achia mediated cytopl.smic incompatibil.ties that may be expl.ited in area-wide pest management.

关键词： Anastrephafratercul.s Anastrepha obl.qua Hal.ane's rul. introgression prezygotic isol.tion postzygotic isol.tion

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The Arabidopsis B-box protein BZS1/BBX20 interacts with HY5 and mediates strigol.ctone regul.tion of photomorphogenesis

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Journal.of Genetics and Genomics 2016年第9期43卷 555-563页

作者： Chuang-Qi Wei Chih-Wei Chien l.an-Feng Ai Jun Zhao Zhenzhen Zhang Kathy H. l. alma l. Burl.ngame Yu Sun Zhi-Yong Wang Key l.boratory of Mol.cul.r and Cel.ul.r Biol.gy of Ministry of Education Hebei Collaboration Innovation Center for Cell Signaling Hebei Key Laboratory of Molecular and Cellular Biology College of Life Sciences Hebei Normal University Shijiazhuang 050024 China Department of Pl.nt Biol.gy Carnegie Institution for Science Stanford CA 94305 USA Hebei Entry-Exit Inspection and Quarantine Bureau of the Peopl.'s Republ.c of China Shijiazhuang 050051 China Center of Basic Forestry and Proteomics Haixia Institute of Science and Technology Fujian Agriculture and Forestry University Fuzhou 350002 China Department of Pharmaceutical.Chemistry University of California San Francisco CA 94158 USA

Pl.nt growth is control.ed by integration of hormonal.and l.ght-signal.ng pathways. BZS1 is a B-box zinc finger protein previousl. characterized as a negative regul.tor in the brassinosteroid （BR）-signal.ng pathway and a positive regul.tor in the l.ght-signal.ng pathway. However, the mechanisms by which BZS1/BBX20 integrates l.ght and hormonal.pathways are not ful.y understood. Here, using a quantitative proteomic workfl.w, we identified several.BZSl.associated proteins, incl.ding l.ght-signal.ng compo- nents COP1 and HY5. Direct interactions of BZS1 with COPI and HY5 were verified by yeast two-hybrid and co-immunoprecipitation assays. Overexpression of BZS1 causes a dwarf phenotype that is sup- pressed by the by5 mutation, whil. overexpression of BZSI fused with the SRDX transcription repressor domain （BZS1-SRDX） causes a l.ng-hypocotyl.phenotype simil.r to by5, indicating that BZSI＇s function requires HY5. BZSI positivel. regul.tes HY5 expression, whereas HY5 negativel. regul.tes BZS1 protein l.vel. forming a feedback l.op that potential.y contributes to signal.ng dynamics. In contrast to BR, strigol.ctone （Sl. increases BZS1 l.vel. whereas the Sl.responses of hypocoryl.el.ngation, chl.rophyl. and HY5 accumul.tion are diminished in the BZSI-SRDX seedl.ngs, indicating that BZS1 is invol.ed in these Sl.responses. These resul.s demonstrate that BZS1 interacts with HY5 and pl.ys a central.rol. in integrating l.ght and mul.ipl. hormone signal. for photomorphogenesis in Arabidopsis.

关键词： l.ght signal.ng l.munoprecipitation Mass spectrometry Strigol.ctone BZS1/BBX20 HY5 COP1

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