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Relationship between Dual-Energy X-Ray Absorptiometry-Derived Appendicular Lean Tissue Mass and Total Body Skeletal Muscle Mass Estimated by Ultrasound

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International Journal of Clinical Medicine 2013年第6期4卷 283-286页

作者： Takashi Abe nicole C. Dabbs Vinayak K. nahar M. allison Ford Martha A. Bass Mark Loftin Department of Health Exercise Science & Recreation Management School of Applied Science The University of Mississippi Oxford USA

Dual-energy X-ray absorptiometry (DXA) is an attractive method for evaluating sarcopenia, age-related loss of skeletal muscle mass and function, using appendicular lean tissue (aLT) mass for criteria of diagnosis, although minimal radiation is exposed. Skeletal muscle (SM) mass can be estimated by using ultrasound-measured muscle thickness (MTH). However, the association between these two methods is unclear. To examine the relationship between DXA-derived aLT mass and total body SM mass estimated by ultrasound, thirty-six healthy adults (18 men and 18 women) aged 19 - 65 years participated in this study. Ultrasound-measured muscle thickness was used to estimate the total SM mass. DXA was used to estimate whole body and regional body composition, and aLT mass was considered equivalent to the sum of lean tissue in both the right and left arms and legs. Total SM mass (26.3 ± 4.4 kg for men and 15.7 ± 2.6 kg for women) estimated by ultrasound was similar to DXA-estimated aLT mass (24.5 ± 3.8 kg for men and 15.7 ± 2.7 kg for women). There was a strong correlation between DXA-measured aLT mass and total SM mass estimated by ultrasound in men (r = 0.927, n = 18) and women (r = 0.931, n = 18) as well as overall sample (r = 0.975, n = 36). The ratio of total SM mass to aLT mass was 1.07 for men and 1.00 for women. These results suggest that DXA-derived aLT mass can be accurately predicted from ultrasound estimated total SM mass, although the predicted value may underestimate in men (approximately 7% at the group level).

关键词： Body Composition Skeletal Muscle Mass Sarcopenia B-Mode Ultrasonography

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Spinal cord injury reprograms muscle fibroadipogenic progenitors to form heterotopic bones within muscles

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Bone Research 2022年第2期10卷 310-325页

作者： Hsu-Wen Tseng Dorothée Girard Kylie A.Alexander Susan M.Millard Frédéric Torossian Adrienne Anginot Whitney Fleming Jules Gueguen Marie-Emmanuelle Goriot Denis Clay Beulah Jose Bianca nowlan allison R.Pettit Marjorie Salga François Genêt Marie-Caroline Le Bousse-Kerdilès Sébastien Banzet Jean-Pierre Lévesque Mater Research Institute-The University of Queensland WoolloongabbaQLDAustralia Institut de Recherche Biomédicale des Armées(IRBA) INSERM UMRS-MD1197 ClamartFrance InSERM UMRS-MD 1197 Universitéde Paris-SaclayHôpital Paul BrousseVillejuifFrance InSERM UMS-44 Universitéde Paris-SaclayHôpital Paul BrousseVillejuifFrance UPOH(UnitéPéri Opératoire du Handicap Perioperative Disability Unit)Physical and Rehabilitation Medicine departmentRaymond-PoincaréHospitalAssistance Publique-Hôpitaux de Paris(AP-HP)GarchesFrance Universitéde Versailles Saint Quentin en Yvelines UFR Simone Veil-SantéEND:ICAP INSERM U1179Montigny le BretonneuxFrance

The cells of origin of neurogenic heterotopic ossifications(nHOs), which develop frequently in the periarticular muscles following spinal cord injuries(SCIs) and traumatic brain injuries, remain unclear because skeletal muscle harbors two progenitor cell populations: satellite cells(SCs), which are myogenic, and fibroadipogenic progenitors(FAPs), which are mesenchymal. Lineage-tracing experiments using the Cre recombinase/Lox P system were performed in two mouse strains with the fluorescent protein Zs Green specifically expressed in either SCs or FAPs in skeletal muscles under the control of the Pax7 or Prrx1 gene promoter, respectively. These experiments demonstrate that following muscle injury, SCI causes the upregulation of PDGFRα expression on FAPs but not SCs and the failure of SCs to regenerate myofibers in the injured muscle, with reduced apoptosis and continued proliferation of muscle resident FAPs enabling their osteogenic differentiation into nHOs. no cells expressing Zs Green under the Prrx1 promoter were detected in the blood after injury, suggesting that the cells of origin of nHOs are locally derived from the injured muscle. We validated these findings using human nHO biopsies. PDGFRα+mesenchymal cells isolated from the muscle surrounding nHO biopsies could develop ectopic human bones when transplanted into immunocompromised mice, whereas CD56+myogenic cells had a much lower potential. Therefore, nHO is a pathology of the injured muscle in which SCI reprograms FAPs to undergo uncontrolled proliferation and differentiation into osteoblasts.

关键词： progenitor injuries muscles

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Agrobacterium tumefaciens: A Bacterium Primed for Synthetic Biology

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BioDesign Research 2020年第1期2020卷 91-106页

作者： Mitchell G.Thompson William M.Moore niklas F.C.Hummel allison n.Pearson Collin R.Barnum Henrik V.Scheller Patrick M.Shih Joint BioEnergy Institute EmeryvilleCAUSA Environmental Genomics and Systems Biology Division Lawrence Berkeley National LaboratoryBerkeleyCAUSA Department of Plant Biology University of California-DavisDavisCAUSA Department of Plant and Microbial Biology University of California-BerkeleyBerkeleyCAUSA Genome Center University of California-DavisDavisCAUSA

Agrobacterium tumefaciens is an important tool in plant biotechnology due to its natural ability to transfer DnA into the genomes of host *** manipulations of *** have yielded considerable advances in increasing transformational efficiency in a number of plant species and ***,there is overwhelming evidence that modulating the expression of various mediators of *** virulence can lead to more successful plant transformation;thus,the application of synthetic biology to enable targeted engineering of the bacterium may enable new opportunities for advancing plant *** this review,we highlight engineering targets in both *** and plant hosts that could be exploited more effectively through precision genetic control to generate high-quality transformation events in a wider range of host *** then further discuss the current state of *** and plant engineering with regard to plant transformation and describe how future work may incorporate a rigorous synthetic biology approach to tailor strains of *** used in plant transformation.

关键词： transformation enable rigorous

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在血管疾病患者中肾小球滤过率和n-末端脑利钠肽前体为心血管死亡的预测因素

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世界核心医学期刊文摘(心脏病学分册) 2007年第9期 58-59页

作者： Shadman R. allison M.A. Criqui M.H. 赵君 University of California San Diego San Diego CA United States

目的:评价肾小球滤过率(GFR)和n-末端脑利钠肽前体(nT-proBnP)对血管疾病患者中死亡终点的预后预测作用。背景:据报道,无论传统危险因素如何,

关键词：脑利钠肽前体预测因素心血管血管疾病患者滤过

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氮与自然

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AMBIO－人类环境杂志 2002年第2期31卷 97-101页

作者： Peter M.Vitousek Stephan Htttenschwiler Lydia Olander Steven allison 王胜斯坦福大学生物科学系巴赛尔大学植物学研究所

在某种程度上,人类对全球氮循环的改变是很重要的,因为增加的氮极大地改变了许多天然生态系统的组成、生产率和其它性质。增加的氮为什么有这么大的影响力?为什么如此多的天然生态系统都处于缺氮状态?减缓氮相对于其它元素的循环过程,... 详细信息

在某种程度上,人类对全球氮循环的改变是很重要的,因为增加的氮极大地改变了许多天然生态系统的组成、生产率和其它性质。增加的氮为什么有这么大的影响力?为什么如此多的天然生态系统都处于缺氮状态?减缓氮相对于其它元素的循环过程,和控制生态系统水平的氮输入输出过程,都使氮供应成为生态系统动态的限制因子。我们讨论了可以减慢氮循环的因子陆地植物和其它有机体之间的化学计量学差异,蛋白质沉淀防御植物的多度,土壤有机质中C-n键状态。对输入来讲,氮固定的能量消耗及其后果,氮以外的养分供应,对固氮者的优先取食,所有这些都能限制生物固氮者的多度和/或活动。这些过程加在一起,推动和维持着许多天然陆地生态系统的氮限制。

关键词：氮循环输入输出生物氮固定人类氮自然

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Acute care needs in an Indian emergency department： A retrospective analysis

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World Journal of Emergency Medicine 2016年第3期7卷 191-195页

作者： Elizabeth G. Clark Jessica Watson allison Leemann Alan H. Breaud Frank G. Feeley III James Wolff Tamorish Kole Gabrielle A. Jacquet Kerala Institute of Medical Sciences Trivandrum Kerala India Boston University School of Public Health Boston Massachusetts USA Department of Emergency Medicine Boston Medical Center Boston Massachusetts USA Boston University Center for Global Health and Development Boston Massachusetts USA Max Hospital New Delhi India Boston University School of Medicine Boston Massachusetts USA

BACKGROUnD: Emergencies such as road traffic accidents(RTAs), acute myocardial infarction(AMI) and cerebrovascular accident(CVA) are the most common causes of death and disability in India. Robust emergency medicine(EM) services and proper education on acute care are necessary. In order to inform curriculum design for training programs, and to improve the quality of EM care in India, a better understanding of patient epidemiology and case burden presenting to the emergency department(ED) is ***: This study is a retrospective chart review of cases presenting to the ED at Kerala Institute of Medical Sciences(KIMS), a private hospital in Trivandrum, Kerala, India, from november 1, 2011 to April 21, 2012 and from July 1, 2013 to December 21, 2013. De-identified charts were systematically sampled and ***: A total of 1 196 ED patient charts were analyzed. Of these patients, 55.35%(n=662) were male and 44.7%(n=534) were female. The majority(67.14%, n=803) were adults, while only 3.85%(n=46) were infants. The most common chief complaints were fever(21.5%, n=257), renal colic(7.3%, n=87), and dyspnea(6.9%, n=82). The most common ED diagnoses were gastrointestinal(15.5%, n=185), pulmonary(12.3%, n=147), tropical(11.1%, n=133), infectious disease and sepsis(9.9%, n=118), and trauma(8.4%, n=101).COnCLUSIOn: The patient demographics, diagnoses, and distribution of resources identifi ed by this study can help guide and shape Indian EM training programs and faculty development to more accurately refl ect the burden of acute disease in India.

关键词： Emergency care systems Emergency department Education Acute care Emergency department utilization

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由放牧压力所影响的肉牛的饲草进食

由放牧压力所影响的肉牛的饲草进食

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“加强世界饲草——牲畜系统”第十四届国际草地会议

作者： C.D.allison M.M.KOTHMAnn L.R.RITTEnHOUSE 李鸥 new Mexico State University Las CrncesN.M.U.S.A

在1977年做了一系列的试验,来确定放牧压力水平对有机物质进食量的影响,以及进食量与饲草消失之间的关系。在四、七和九月的14天试验中所研究的放牧压力水平为每天给每个家畜单位提供10、20、40和50公斤饲草（公斤/家畜单位/天）。每次...

在1977年做了一系列的试验,来确定放牧压力水平对有机物质进食量的影响,以及进食量与饲草消失之间的关系。在四、七和九月的14天试验中所研究的放牧压力水平为每天给每个家畜单位提供10、20、40和50公斤饲草（公斤/家畜单位/天）。每次试验之前,中期以及之后都要测定饲草的现有量。有机物质进食量是用排粪量:未消化率比率技术来估计的。有机物质进食量在四月份最高;肉牛在九月份消耗的饲草比七月份多。把饲草质量与家畜生理状况结合起来可以解释试验中进食量的变化。肉牛所消耗的有机物质在试验结束时要比开始时大约要少12克/公斤代谢体重（kg体重0.75）。在10和20公斤/家畜单位/天的处理中,肉牛在试验结束时有比在开始时消耗的饲草减少的趋势。总的现存量在放牧试验中稳定地下降,饲草的可利用率在试验结束时也随之显著少于开始时和中期。三个试验平均起来,在放牧压力为10、20、40和50公斤/家畜单位/天时,饲草的消失分别为8.5、12.0、12.7和16.3公斤/家畜单位/天。然而对于所有的处理,期间和试验来说,平均每天的进食量约为9公斤/家畜单位/天。当放牧压力的水平为10公斤/家畜单位/天时,饲草的消失约等于平均的日进食量,而在放牧压力为20、40和50公斤/家畜单位/天的条件下,饲草的消失则分别超过进食量28%、48%和90%。这些数据表明,当放牧压力增加时放牧肉牛的饲草收获效率有可能增长2倍。

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人参减少 γ射线照射的淋巴细胞微核量(英文)

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新乡医学院学报 2004年第3期21卷 153-159页

作者：李同光 Ron R allison Kevin F O'Brien Prabhaker G Khazanie Larry J Dobbs Jr. Peter J Kragel 东卡罗莱纳大学Brody医学院东卡罗莱纳大学卫生学院

目的　评价中国人参对减少辐射诱导的人周围血 G0 期淋巴细胞 (PBL)微核量的作用。方法　采用细胞因子阻滞的微核法检测健康志愿者的血标本。体外 1 37Cs照射之前 ,每份血标本的单个核细胞培养以不同浓度 (0～ 2 0 0 0μg· m l- 1 )的... 详细信息

目的　评价中国人参对减少辐射诱导的人周围血 G0 期淋巴细胞 (PBL)微核量的作用。方法　采用细胞因子阻滞的微核法检测健康志愿者的血标本。体外 1 37Cs照射之前 ,每份血标本的单个核细胞培养以不同浓度 (0～ 2 0 0 0μg· m l- 1 )的人参根水提取物孵育 2 4 h。结果　 (1)在 0 Gy时 ,每 10 0 0个双核 (Bn )细胞的微核量是 11.3± 2 .7,人参水提取物≥ 5 0 0μg· ml- 1 时微核量下降 ,但微核量和人参水提取物的浓度之间的差异是显著的。 (2 )单独射线照射显著增加微核量 ,当暴露于 1Gy的照射时 ,随着培养介质中人参水提取物浓度的增加 ,微核量呈显著的线性降低 (R2 =0 .0 5 ,P =0 .0 0 2 ) ;对暴露于 1Gy辐射诱导的微核量 ,人参水提取物浓度在 15 0 0μg· m l- 1 时减少 4 6 .6 % ,浓度在 2 0 0 0μg·m l- 1时减少 5 7.6 % ;对暴露于 2 Gy辐射诱导的微核量的减少呈更强的线性相关 (R2 =0 .7,P=0 .0 0 0 1) ;(3)微核数据分析的结果呈现出与 Poisson模型相关的过度离散趋势。结果表明 :(1)人参水提取物浓度达 2 0 0 0 μg· ml- 1时 ,对 PBL 细胞毒作用无明显影响 ;(2 )人参水提取物浓度在 5 0 0 μg· ml- 1和 2 0 0 0 μg· ml- 1之间时 ,对抗 1 37Cs照射诱导的 Mn发挥保护作用。

关键词：人参辐射防护微核淋巴细胞

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放疗前周围血淋巴细胞敏感性是骨盆放疗所致疾病的预示因素(英文)

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新乡医学院学报 2002年第6期19卷 445-455页

作者：李同光 Ron R.allison Kevin F.O’Brien Roberta M.Johnke Karen I.Christie James L.naves Ulf L.Karlsson Leo W.Jenkins癌症中心放射肿瘤科东卡罗来纳大学公共卫生学院生物统计中心

目的　探讨前列腺癌 (PC)治疗前体外照射诱导的Go淋巴细胞微核量是否与放疗 (xRT)相关疾病的发生有关。方法　对 3 8例PC周围血淋巴细胞 (PBL)以细胞因子阻止的微核法进行前瞻性观察。患者平均年龄 68.7± 1.0岁。临床分期 :T116例 ,T2... 详细信息

目的　探讨前列腺癌 (PC)治疗前体外照射诱导的Go淋巴细胞微核量是否与放疗 (xRT)相关疾病的发生有关。方法　对 3 8例PC周围血淋巴细胞 (PBL)以细胞因子阻止的微核法进行前瞻性观察。患者平均年龄 68.7± 1.0岁。临床分期 :T116例 ,T2 16例 ,T36例。治疗前前列腺癌特异性抗原 (PSA)≤ 4ng·ml-1者 5例 ,4～ 10ng·ml-1者 19例 ,>10ng·ml-1者 14例。所有患者均接受标准的骨盆外xRT(41.4～ 5 0 .4Gy) ,追加剂量 (16.4～ 2 6Gy)。平均随访 3 2 .8± 4.6个月。随访末 ,按RTOG标准对胃肠道 (GI)或泌尿生殖道 (GU)xRT相关疾病的发生率进行评定。结果　发生xRT相关疾病 2 4例为高敏 (HS) ,未发生者 14例为正常敏感性 (nS) ,HS患者均有GI(1～ 3级 )或GU(1～ 2级 )xRT相关疾病。体外照射暴露的血标本 ,与照射剂量相关的PBL微核量在HS和nS患者之间有显著差异 ,特别是当剂量≥ 3Gy时。xRT相关疾病的发生与治疗前PBL微核量相关 ,而与病情无关。

关键词：放疗周围血淋巴细胞微核剂量反应关系前列腺癌

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Variable change in renal function by hypertonic saline

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World Journal of Critical Care Medicine 2014年第2期3卷 61-67页

作者： Jesse J Corry Panayiotis Varelas Tamer Abdelhak Stacey Morris Marlisa Hawley allison Hawkins Michelle Jankowski Department of neurology Henry Ford Hospital Department of neurosurgery Henry Ford Hospital Department of Biostatistics Henry Ford Hospital

AIM: To investigate the effects of hypertonic saline in the neurocritical care ***: We retrospectively reviewed our hospital's use of hypertonic saline(HS) since March of 2005, and prospectively since October 2010. Comparisons were made between admission diagnoses, creatinine change(Cr), and HS formulation(3% na Cl, 3% na Cl/sodium acetate mix, and 23.4% na Cl) to patients receiving normal saline or lactated ringers. The patients(n = 1329) of the retrospective portion were identified. The data presented represents the first 230 patients with data. RESULTS: Significant differences in Acute Physiology and Chronic Health Evaluation Ⅱ scores and GlasgowComa Scale scores occurred between different saline formulations. no significant correlation of Cl- or na+ with Cr, nor with saline types, occurred. When dichotomized by diagnosis, significant correlations appear. Traumatic brain injury(TBI) patients demonstrated moderate correlation between na+ and Cr of 0.45. Stroke patients demonstrated weak correlations between na+ and Cr, and Cl- and Cr(0.19 for both). Patients receiving HS and not diagnosed with intracerebral hemorrhage, stroke, subarachnoid hemorrhage, or TBI demonstrated a weak but significant correlation between Cl- and Cr at ***: Cr directly correlates with na+ or Cl- in stroke, na+ in TBI, and Cl- in other populations. Prospective comparison of HS and renal function is needed.

关键词： Hypertonic saline solution Sodium chloride Acute kidney injury Cerebral edema Critical care

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