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Decoding altitude-activated regulatory mechanisms occurring during apple peel ripening

Horticulture research 2020年第1期7卷 1149-1160页

作者： Evangelos Karagiannis Michail Michailidis Georgia Tanou federico Scossa Eirini Sarrou George Stamatakis Martina Samiotaki Stefan Martens alisdair r.f.rnie Athanassios Molassiotis Laboratory of Pomology Department of AgricultureAristotle University of Thessaloniki54124 ThessalonikiGreece Institute of Soil and Water resources ELGO-DEMETERThermiThessaloniki 57001Greece Max-Planck-Institute of Molecular Plant Physiology Am Müehlenberg 1.Potsdam-Golm 14476Germany Council for Agricultural research and Economics Research Center for Genomics and BioinformaticsVia Ardeatina 54600178 RomeItaly Institute of Plant Breeding and Genetic resources ELGO-DEMETERThermiThessaloniki 57001Greece Biomedical Sciences research Center“Alexander fleming” Vari 16672Greece fondazione Edmund Mach Centro Ricerca e InnovazioneDepartment of Food Quality and NutritionVia E.Mach138010 San Michele all’AdigeTNItaly

Apple(Malus domestica Borkh)is an important fruit crop cultivated in a broad range of environmental *** fruit ripening is a physiological process,whose molecular regulatory network response to different environments is still not sufficiently investigated and this is particularly true of the peel *** this study,the influence of environmental conditions associated with low(20 m)and high(750 m)altitude on peel tissue ripening was assessed by physiological measurements combined with metabolomic and proteomic analyses during apple fruit development and *** apple fruit ripening was itself not affected by the different environmental conditions,several key color parameters,such as redness and color index,were notably induced by high *** with this observation,increased levels of anthocyanin and other phenolic compounds,including cyanidin-3-O-galactoside,quercetin-3-O-rhamnoside,quercetin-3-O-rutinoside,and chlorogenic acid were identified in the peel of apple grown at high ***,the high-altitude environment was characterized by elevated abundance of various carbohydrates(e.g.,arabinose,xylose,and sucrose)but decreased levels of glutamic acid and several related proteins,such as glycine hydroxymethyltransferase and glutamate–glyoxylate *** processes affected by high altitude were the TCA cycle,the synthesis of oxidative/defense enzymes,and the accumulation of photosynthetic *** the obtained data we were able to construct a metabolite-protein network depicting the impact of altitude on peel *** combined analyses presented here provide new insights into physiological processes linking apple peel ripening with the prevailing environmental conditions.

关键词： altitude network prevailing

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Tecia solanivora infestation increases tuber starch accumulation in Pastusa Suprema potatoes

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Journal of Integrative Plant Biology 2018年第11期60卷 1083-1096页

作者： Pavan Kumar Etzel Garrido Kun Zhao Yi Zheng Saleh Alseekh Erandi Vargas-Ortiz alisdair r.f.rnie Zhangjun fei Katja Poveda Georg Jander Boyce Thompson Institute for Plant research 533 Tower RoadIthacaNY 14853USA Department of Entomology Cornell UniversityIthacaNY 14853USA Max Planck Institute of Molecular Plant Physiology Wissenschaftspark GolmAm Muhlenberg 114476 PotsdamGermany department of entomology and nematology university of floridaGainesvilleFL 32611USA unidad de microbiologia basica y aplicada facultad de ciencias naturalesuniversidad autonoma de queretaroAv.de las Ciencias SINJuriquillaquerdtaroMdxico department of molecular biology institute for scientific and technological research of san luis potosiMexico

In response to infestation with larvae of the Guatemalan tuber moth （Tecia solanivora）, some Solanum tuberosum （potato） varieties exhibit an overcompensa- tion response, whereby the total dry mass of uninfested tubers is increased. Here, we describe early responses, within the first few days, of T. solanivora feeding, in the Colombian potato variety Pastusa Suprema. Non- targeted metabolite profiling showed significant second- ary metabolism changes in T. solanivora-infested tubers, but not in uninfested systemic tubers. In contrast, changes in primary metabolism were greater in unin- fested systemic tubers than in the infested tubers, with a notable 80% decline in systemic tuber sucrose levels within 1 d of T. solanivora infestation. This suggested either decreased sucrose transport from the leaves or increased sink strength, i.e., more rapid sucrose to starch conversion in the tubers. Increased sucrose synthesis was indicated by higher rubisco activase and lower starch synthase gene expression in the leaves of infested plants. Elevated sink strength was demonstrated by 45% more total starch deposition in systemic tubers of T. solanivord- infested plants compared to uninfested control plants. Thus, rather than investing in increased defense of uninfested tubers, Pastusa Suprema promotes deposition of photoassimilates in the form of starch as a response to T. solanivora infestation.

关键词： Tecia solanivora infestation increases tuber starch accumulation in Pastusa Suprema potatoes figure

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A Highly Efficient Agrobacterium-Mediated Method for Transient Gene Expression and functional Studies in Multiple Plant Species

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Plant Communications 2020年第5期1卷 129-140页

作者： Youjun Zhang Moxian Chen Beata Siemiatkowska Mitchell rey Toleco Yue Jing Vivien Strotmann Jianghua Zhang Yvonne Stahl alisdair r.f.rnie Center of Plant Systems Biology and Biotechnology 4000 PlovdivBulgaria Max-Planck-Institut fur Molekulare Pflanzenphysiologie Am Muhlenberg 114476 Potsdam-GolmGermany Department of Biology Hong Kong Baptist Universityand State Key Laboratory of AgrobiotechnologyThe Chinese University of Hong KongShatinHong Kong Institute for Developmental Genetics Heinrich Heine UniversityDusseldorfGermany

Although the use of stable transformation technology has led to great insight into gene function,its application in high-throughput studies remains ***-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and protein interaction analysis,but this technique does not work efficiently in other plant species,including Arabidopsis *** an efficient high-throughput transient expression system is currently lacking in the model plant species ***,we developed a method that is characterized by high efficiency,reproducibility,and suitability for transient expression of a variety of functional proteins in *** and 7 other plant species,including Brassica oleracea,Capsella rubella,Thellungiella salsuginea,Thellungiella halophila,Solanum tuberosum,Capsicum annuum,and *** of this method was independently verified in three independent research facilities,pointing to the robustness of this ***,in addition to demonstrating the utility of this technique in a range of species,we also present a case study employing this method to assess protein–protein interactions in the sucrose biosynthesis pathway in Arabidopsis.

关键词： transient expression agro-infiltration subcellular localization protein-protein interaction

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Metabolons,enzyme-enzyme assemblies that mediate substrate channeling,and their roles in plant metabolism

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Plant Communications 2021年第1期2卷 13-28页

作者： Youjun Zhang alisdair r.f.rnie Center of Plant Systems Biology and Biotechnology 4000 PlovdivBulgaria Max-Planck-Institut für Molekulare Pflanzenphysiologie Am Mühlenberg 114476 Potsdam-GolmGermany

Metabolons are transientmulti-protein complexes of sequential enzymes that mediate substrate *** differ from multi-enzyme complexes in that they are dynamic,rather than permanent,and as such have considerably lower dissociation *** the fact that a huge number of metabolons have been suggested to exist in plants,most of these claims are erroneous as only a handful of these have been proven to *** believe that physical protein-protein interactions between consecutive enzymes of a pathway should rather be called enzyme-enzyme *** this review,we describe how metabolons are generally assembled by transient interactions and held together by both structural elements and non-covalent *** evidence for their existence comes fromprotein-protein interaction studies,which indicate that the enzymes physically interact,and direct substrate channelingmeasurements,which indicate that they functionally ***,advances in cell biology and proteomics have far outstripped those in classical enzymology and flux measurements,rendering most reports reliant purely on interactome *** developments in co-fractionation mass spectrometry will likely further exacerbate this *** this,only dynamic enzyme-enzyme assemblies in which both physical and functional interactions have been demonstrated should be termed *** discuss the level of evidence for the manifold plant pathways that have been postulated to contain metabolons and then list examples in both primary and secondary metabolism for which strong evidence has been provided to support these *** doing so,we pay particular attention to experimental and mathematical approaches to study metabolons as well as complexities that arise in attempting to follow ***,we discuss perspectives for improving our understanding of these fascinating but enigmatic interactions.

关键词： protein-protein interaction metabolon substrate channeling

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Quantitative Trait Loci Analysis Identifies a Prominent Gene Involved in the Production of fatty Acid-Derived flavor Volatiles in Tomato

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Molecular Plant 2018年第9期11卷 1147-1165页

作者： Karolina Garbowicz Zhongyuan Liu Saleh Alseekh Denise Tieman Mark Taylor Anastasiya Kuhalskaya Itai Ofner Dani Zamir Harry J. Klee alisdair r. fernie Yariv Brotman Max-Planck-lnstitute of Molecular Plant Physiology Potsdam-Golm Germany Horticultural Sciences Plant Innovation Center University of Florida Gainesville FL USA Center of Plant System Biology and Biotechnology 4000 Plovdiv Bulgaria Department of Life Sciences Ben-Gurion University of the Negev Beersheba Israel robert H. Smith Institute of Plant Sciences and Genetics Faculty of Agriculture Hebrew University of Jerusalem Rehovot 7610001 Israel These authors contributed equally to this article

To gain insight into the genetic regulation of lipid metabolism in tomato, we conducted metabolic trait loci （mQTL） analysis following the lipidomic profiling of fruit pericarp and leaf tissue of the Solanum pennellii introgression lines （IL）. To enhance mapping resolution for selected fruit-specific mQTL, we profiled the lipids in a subset of independently derived S. pennellii backcross inbred lines, as well as in a nearsogenic sub-iL population. We identified a putative lecithin：cholesterol acyltransferase that controls the levels of several lipids, and two members of the class III lipase family, LIP1 and LIP2, that were associated with decreased levels of diacylglycerols （DAGs） and triacylglycerols （TAGs）. Lipases of this class cleave fatty acids from the glycerol backbone of acylglycerols. The released fatty acids serve as precursors of flavor volatiles. We show that LIP1 expression correlates with fatty acid-derived volatile levels. We further confirm the function of LIP1 in TAG and DAG breakdown and volatile synthesis using transgenic plants. Taken together, our study extensively characterized the genetic architecture of Upophilic compounds in tomato and demonstrated at molecular level that release of free fatty acids from the glycerol backbone can have a major impact on downstream volatile synthesis.

关键词： lipidomics lipids lipase volatile fA-VOC Z-4-decenal

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OsGf14b modulates defense signaling pathways in rice panicle blast response

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The Crop Journal 2021年第4期9卷 725-738页

作者： Shijuan Yan Qing Liu Thomas Naake Wenjie Huang Mengyu Chen Qian Kong Sheng Zhang Wenyan Li Xuan Li Qinjian Liu Jianyuan Yang alisdair r.f.rnie Bin Liu Guangdong Key Laboratory for Crop Germplasm resources Preservation and Utilization Agro-biological Gene Research Center Guangdong Academy of Agricultural Sciences Guangdong Key Laboratory of New Technology in rice Breeding Rice Research Institute Guangdong Academy of Agricultural Sciences Max Planck Institute of Molecular Plant Physiology 4. Proteomics and Metabolomics facility Institute of Biotechnology Cornell University Guangdong Key Laboratory of New Technology in Plant Protection Plant Protection Research Institute Guangdong Academy of Agricultural Sciences

rice with panicle-blast resistance is needed for stable rice production. Although we have previously demonstrated that OsGf14 b underlies a quantitative trait locus that positively regulates rice panicle blast resistance, the mechanism is still unknown. In this study, a multi-omics approach was used to investigate the possible downstream signaling pathway regulated by Os Gf14 b. OsGf14 b both strongly activated the gibberellin biosynthetic pathway during pathogen infection and reprogrammed the lignin biosynthetic pathway. reduced lignin accumulation was observed in glumes of OsGf14 b-overexpressing plants in comparison with the wild type after pathogen inoculation. OsGf14 b activated the auxin and jasmonic acid signaling pathways, but inactivated the salicylic acid signaling pathway. Auxin and jasmonic acid appeared to act independently on OsGf14 b-mediated panicle blast resistance. The roles of gibberellin, lignin, and auxin were different from their roles in leaf blast, suggesting that different mechanisms underlie leaf and panicle blast resistance in rice. This study provides a comprehensive catalog of molecular changes that could be targets for future studies of rice panicle blast resistance.

关键词： rice Panicle blast resistance OsGf14b Gibberellin Phytohormone

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Mapping theArabidopsis Metabolic Landscape by Untargeted Metabolomics at Different Environmental Conditions

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Molecular Plant 2018年第1期11卷 118-134页

作者： Si Wu Takayuki Tohge Alvaro Cuadros-lnostroza Hao Tong Hezi Tenenboim rik Kooke Michael Meret Joost B. Keurentjes Zoran Nikoloski alisdair r. fernie Lothar Willmitzer Yariv Brotman 1 Max Planck Institute of Molecular Plant Physiology 14476 Potsdam-Golm Germany MetaSysX GmbH Am Muhlenberg 11 14476 Potsdam-Golm Germany Laboratory of Genetics Wageningen University Droevendaalsesteeg 1 6708 PB Wageningen the Netherlands Department of Life Sciences Ben Gurion University of the Negev Beersheva Israel

Metabolic genome-wide association studies （mGWAS）, whereupon metabolite levels are regarded as traits, can help unravel the genetic basis of metabolic networks. A total of 309Arabidopsis accessions were grown under two independent environmental conditions （control and stress） and subjected to untargeted LC-MS- based metabolomic profiling; levels of the obtained hydrophilic metabolites were used in GWAS. Our two- condition-based GWAS for more than 3000 semi-polar metabolites resulted in the detection of 123 highly resolved metabolite quantitative trait loci （p ≤ 1.0E-08）, 24.39% of which were environment-specific. Interestingly, differently from natural variation in Arabidopsis primary metabolites, which tends to be controlled by a large number of small-effect loci, we found several major large-effect loci alongside a vast number of small-effect loci controlling variation of secondary metabolites. The two-condition-based GWAS was fol- lowed by integration with network-derived metabolite-transcript correlations using a time-course stress experiment. Through this integrative approach, we selected 70 key candidate associations between struc- tural genes and metabolites, and experimentally validated eight novel associations, two of them showing differential genetic regulation in the two environments studied. We demonstrate the power of combining large-scale untargeted metabolomics-based GWAS with time-course-derived networks both performed under different ablotic environments for identifying metabollte-gene associations, providing novel global insights into the metabolic landscape of Arabidopsis.

关键词： untargeted metabolomics GWAS network analysis different environments secondary metabolism

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Establishment of a Photoautotrophic Cell Suspension Culture of Arabidopsis thaliana for Photosynthetic, Metabolic, and Signaling Studies

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Molecular Plant 2012年第2期5卷 524-527页

作者： Christine Hampp Andreas richter Sonia Osorio Günther Zellnig Alok Krishna Sinha Alexandra Jammer alisdair r. fernie Bernhard Grimm Thomas roitsch Julius-von-Sachs-Institut fuer Biowissenschaften UniversitaetWuerzburgJulius-von-Sachs-Platz 297082 WuerzburgGermany Institute of Biology/Plant Physiology Humboldt UniversityPhilippstr.13Building 1210115 Berlin Max-Planck Institute of Molecular Plant Physiology Am Mühlenberg 114476 Potsdam-GolmGermany Institut fuer Pflanzenwissenschaften Schubertstr.51A-8010 GrazAustria National Institute of Plant Genome research PB No.10531Aruna Asaf Ali RoadNew Delhi–110067India

Dear Editor, Plant cell suspension cultures have been used as model systems to circumvent the problems associated with the analyses of a multi-factorial plant that is composed of multiple tissue and cell types exposed to diverse signals. A number of plant suspension cultures have proven to be valuable to study various topics including defense response, secondary metabolite formation, ion transport, gene regulation, and signal duction （roitsch and Sinha, 2002 and references therein）. However, most cultures reported to date, including the cultures from model species such as Arabidopsis （Christie and Jenkins,

关键词： Photosynthetic Metabolic Signaling Studies

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Analysis of the Interface between Primary and Secondary Metabolism in Catharanthus roseus Cell Cultures Using ^13C-Stable Isotope feeding and Coupled Mass Spectrometry

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Molecular Plant 2013年第2期6卷 581-584页

作者： Carla Antonio Natali r. Mustafa Sonia Osorio Takayuki Tohge Patrick Giavalbco Lothar Willmitzer Heiko rischer Kirsi-Marj Oksman-Caldentey robert Verpoorte alisdair r. fernie Central Metabolism Group Max Planck Institute of Molecular Plant Physiology Am Muehlenberg 1 D-14476 Potsdam-Golm Germany Natural Products Laboratory Institute of Biology Leiden University Einsteinweg 55 2300 RA Leiden The Netherlands Present address: Departamento de Biologia Molecular y Bioquimica Universidad de M.~laga 29071 M=~laga Spain Genes and Small Molecules Group Max Planck Institute of MolecularPlant Physiology Am Muehlenberg 1 D-14476 Potsdam-Golm Germany VTT Technical research Centre of finland PO Box 1000 F1-02044 VTT (Espoo) Finland

Dear Editor, The tropical plant Madagascar periwinkle Catharanthus roseus （L.） *** is a rich source of plant-derived medicinal ter-penoid indole alkaloids （TIAs）, including the anti-hypertensive ajmalicine, the sedative compound serpentine, and the anti-cancer drugs vinblastine and vincristine. However, the latter two compounds are produced in C. roseus plants only in very low amounts. Elicitors such as hormones （e.g. jasmonates or salicylic acid） activate plant natural defense responses, includ-ing increased secondary metabolite production （EI-Sayed and Verpoorte, 2007; Lackman et al.. 2011）.

关键词： GAS-CHrOMATOGrAPHY BIOSYNTHESIS ArABIDOPSIS

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Combined Transcriptomics and Metabolomics of Arabidopsis thaliana Seedlings Exposed to Exogenous GABA Suggest Its role in Plants Is Predominantly Metabolic

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Molecular Plant 2014年第6期7卷 1065-1068页

作者： Albert Batushansky Menny Kirma Nicole Grillich David Toubiana Phuong Anh Pham Ilse Balbo Hillel fromm Gad Galili alisdair r. fernie Aaron fait The Jacob Blaustein Institutes for Desert research Ben-Gurion University of the Negev84990 Sede Boqer Campus Israel Department of Plant Science Weizmann Institute of Science 76100 Rehovot Israel Max-Planck Institute of Molecular Plant Physiology Am Mühlenberg 114476 Potsdam-Golm Germany Department of Molecular Biology and Ecology of Plants Faculty of Life Sciences Tel Aviv University 69978 Tel Aviv Israel

Dear Editor, y-Aminobutyric acid （GABA） is a non-protein amino acid （AA） metabolized via the GABA shunt; a three-enzyme pathway that includes glutamate decarboxylase （GAD）, GABA transaminases （GABA-T）, and succinic semialdehyde dehydrogenase （SSADH）（Bown and Shelp, 1997; Bouch~ and fromm, 2004）. The majority of work on GABA, to date, has focused on its role as an inhibitory neurotransmitter in mammals. In plants, however, due to its intermediate posi- tion between carbon （C） and nitrogen （N）, the metabolism of GABA has been suggested as a modulator of C-N balance （fair et al., 2008）. In addition, the numerous observations of a rapid accumulation of GABA in response to （a）biotic stresses has prompted different hypotheses to be postu- lated on its role including a regulator of cytosolic pH and a herbivore deterrent （Bouche and fromm, 2004; roberts, 2007）. Additionally, a signaling role has been hypothesized in plants, though evidence remains elusive and the debate on the dual function of GABA as a signaling molecule and as a metabolite in plants remains ongoing. In the present work, we report changes in the metabolite and transcript profiles in Arabidopsis seedlings exposed to exogenous GABA. Liquid-grown seedlings were cultured under C and N limitation to reveal a possible role of GABA as either C or N substrate and to ascertain its influence on transcriptional programs.

关键词： GABA 代谢物拟南芥外源性转录组植物幼苗 γ-氨基丁酸

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