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Creatine supplementation in exercised rats: Effects on the aerobic capacity

Open Journal of Animal Sciences 2013年第1期3卷 21-26页

作者： Michel Barbosa de Araújo Roberto C. Vieira Junior Leandro P. Moura Marcelo Costa Junior Rodrigo A. dalia Amanda Christine da silva Sponton Carla Ribeiro Maria alice R. Mello Biosciences Institute Department of Physical Education Universidade Estadual Paulista Júlio de Mesquita Filho Rio Claro Brazil Biosciences Institute Department of Physical Education Universidade Estadual Paulista Júlio de Mesquita Filho Rio Claro Brazil Faculty of Nutrition Universidade Federal de Mato Grosso Cuiabá Brazil

This study aimed to analyze the possible metabolic disturbances caused by creatine supplementation on aerobic capacity of rats, inferred by the maximal lactate steady state. Forty male Wistar rats (90 days old) were distributed into two groups for eight weeks: trained group (T): rats that were submitted to a training protocol, and supplemented-trained group (TCr): rats that were submitted to a training protocol and received balanced diet supplemented with 2% creatine. The blood lactate concentrations equivalent to maximal lactate steady state during treadmill running were analyzed at the beginning and also at the end of the experiment. At the end of the experiment were done comparing the test results MLSS between the two groups. At the beginning of the experiment, prior to groups division, the majority of animals obtained MLSS at a speed of26 m/min, blood lactate concentration of 3.79 ± 0.76 mmol/L. At the end of the experiment, most of trained rats in T presented MLSS at the speed of28 m/min, blood lactate concentration of 3.37 ± 0.68 mmol/L. Most TCr had MLSS at the speed of28 m/min, blood lactate concentration of 3.52 ± 0.69 mmol/L. We conclude that creatine supplementation was not the cause of the improvement in the aerobic capacity of rats in the tread-mill exercise.

关键词： Diet Lactate Maximal Lactate Steady State Treadmill Running

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Phytotoxic Effects of 4-Chlorophenol and 2,4-Dichlorophenol in the Germination of Seeds of Phaseolus vulgarisand Zea mayz

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American Journal of Plant Sciences 2021年第4期12卷 614-623页

作者： Irasema Pérez-Portuondo Manuel Serrat-Díaz rosa M. Pérez-silva Arelis Ábalos-Rodríguez Industrial Biotechnology Studies Center University of Orient Santiago de Cuba Cuba

Soil contaminated with pesticides may reduce plant development due to their toxicity. The aim of this study was to evaluate the influence on the germination of Zea mayz and Phaseolus vulgaris of the two main intermediates of the 2,4-D degradation, which are 4-chlorophenol (4-CP) and 2,4-dichlorophenol (2,4-DCP). Maize and bean seeds were treated with distilled water (control treatment) and increased concentrations of 4-CP and 2,4-DCP (0.006, 0.1, 1.0, 1.5 g·L-1). It was assessed seed germination and calculated various parameters. The parameter most affected by chlorophenols was the index of germination rate, being the P. vulgaris seeds most affected. 2,4-DCP was the compound most toxic for both plants. The germination index was dependent doses for both plant models tested. The results indicate that 4-CP and 2,4-DCP affected the index of germination rate but not influenced in other parameters of germination of Zea mayz and Phaseolus vulgaris. Maize was most tolerance to both chlorophenols in the assessed concentrations. 2,4-DCP dana;">was the most toxic of chlorophenols tested.

关键词： Chlorophenol Bean Maize Phytotoxicity Seed Germination

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Evaluation of an internal control in a multiplex-PCR assay for sex determination of in vitro-produced bovine embryos

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Advances in Bioscience and Biotechnology 2011年第6期2卷 456-462页

作者： Fabiana de Almeida Rufino Marcelo Marcondes Seneda alice Fernandes Alfieri Katia Cristina silva-Santos Karina Cristina Puggesi Rubin Amauri Alcindo Alfieri 不详

The use of an internal control in a multiplex-PCR assay for sex determination of In Vitro-produced bovine embryos was evaluated in biopsies of groups of 54 fresh and 44 frozen embryos. The internal controls used were the primers BOV 1 and BOV 2, which amplify a product with 626 base pairs (bp) of bovine mitochondrial DNA ND5 gene. The primers BRY.4aF and BRY.4aR were used for bovine Y chromosome sequence amplification. The specificity of multiplex- PCR reactions realized in biopsies corresponding to about 20% of each fresh embryo (10 male and 10 female) by means of confirming the sexing in the remaining embryo content (~80%) presented 100% specificity. Amplicons of the internal control and Y chromosome were both amplified until dilution corresponding to 6.25% of total extracted DNA from a male embryo. Sex determination was possible in 53 (98.1%) fresh embryos and 40 (90.9%) frozen embryos. The products related to the Y chromosome and mitochondrial DNA were simultaneously amplified in 34 (63%) fresh embryos and 27 (61.4%) frozen embryos, showing a male embryo. The female sex, distinguished by internal control amplification only, was detected in 19 (35.2%) and 13 (29.5%) biopsies, respectively, of fresh and frozen embryos. In one (1.8%) and four (9.1%) biopsies of fresh and frozen embryos, respectively, neither product was amplified, most likely due to the absence of embryonic cells or the presence of embryonic cells going through apoptosis. The multiplex-PCR assay developed in this work showed avoided the limitation of a lack of an internal standard, and was also sensitive, specific, and efficient in reaction failure identification. This technique shows great potential for use on a commercial scale in routine sex determination of In Vitro-produced embryos.

关键词： Bovine Embryos In Vitro Production Sexing Multiplex-PCR

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Rainwater Harvesting in Multifamily Social Interest Housing

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Journal of Civil Engineering and Architecture 2015年第10期9卷 1261-1268页

作者： Regina Lucia Melo de Oliveira Marilia Karla da silva Santos Simone rosa da silva Polytechnic School of the University of Pernambuco Recife 50720-001 Brazil

This study evaluated the reduction of water consumption in a SIH (social interest housing) complex from the simulation of implanting a rainwater harvesting system. The methodology consisted of characterization of the case study, survey data of water consumption and data of precipitation in the area, on-site visits to define the average consumption and monthly water distribution, and sizing of a rainwater harvesting tank following the Netuno Program. It was obtained 22.500 L as the ideal volume for the tank, supplying the demand for drinkingwater in 32%, although the reduction in the drinking water bills would be minor, since the object of the case study is considered SIH, and residents pay a fixed fee for consuming up to 10 m3 per month. Therefore, it becomes necessary to analyze this situation from a sustainable and environmental perspective, and the benefits are no longer only economic, but rather they take on a more comprehensive social dimension.

关键词：雨水收集系统社会利益住房收获固定费用调查数据 SIH 复合物

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Correction to:Proposed nomenclature for Pseudallescheria,Scedosporium and related genera

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Fungal Diversity 2022年第2期113卷 193-194页

作者： Michaela Lackner G.Sybren de Hoog Liyue Yang Leandro Ferreira Moreno Sarah A.Ahmed Fritz Andreas Josef Kaltseis Markus Nagl Cornelia Lass-Florl Brigitte Risslegger Gunter Rambach Cornelia Speth Vincent Robert Walter Buzina Sharon Chen Jean-Philippe Bouchara Jose F.Cano-Lira Josep Guarro Josepa Gene Fabiola Fernandez silva rosa Haido Gerhard Haase Vladimir Havlicek Dea Garcia-Hermoso Jacques F.Meis Ferry Hagen Martin Kirchmair Johannes Rainer Katharina Schwabenbauer Mirjam Zoderer Wieland Meyer Felix Gilgado Vania A.Vicente Elena Pieckova Monika Regenermel Peter-Michael Rath Joerg Steinmann Xisto Wellington de Alencar Françoise Symoens Kathrin Tintelnot Krzysztof Ulfig Aristea Velegraki Anna Maria Tortorano Sandrine Giraud Sara Mina Kinga Rigler-Hohenwarter Fernando L.Hernando Andoni Ramirez-Garcia Aize Pellon Jashanpreet Kaur Eliana Barreto Bergter Jardel Vieira de Meirelles Ingrid Dutra da silva Laurence Delhaes Ana Alastruey-Izquierdo Ruo-yu Li Qiaoyun Lu Tarek Moussa Omar Almaghrabi Hassan Al-Zahrani Gen Okada Shuwen Deng Wangqing Liao Jingsi Zeng Jouni Issakainen Livia Cristina Liporagi Lopes Division of Hygiene and Medical Microbiology Innsbruck Medical UniversityInnsbruckAustria CBS Fungal Biodiversity Centre UtrechtThe Netherlands Institute for Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamThe Netherlands Sun Yat-Sen Memorial Hospital Sun Yat-Sen UniversityGuangzhouChina Medical University of Graz GrazAustria Centre for Infectious Diseases and Microbiology Westmead HospitalWestmeadAustralia Laboratoire de Parasitologie-Mycologie Centre Hospitalier UniversitaireAngersFrance Unitats de Microbiologia i d’Anatomia Patologica Facultat de Medicina i Ciencies de la Salut IISPVUniversitat Rovira i VirgiliReusSpain Universidade Federal do Estado do Rio de Janeiro Rio de JaneiroBrazil Institute of Medical Microbiology University Hospital RWTHAachenGermany Institute of Microbiology Academy of SciencesPragueCzech Republic Institut Pasteur Unite de Mycologie MoleculaireCentre National de Reference Mycoses Invasives et AntifongiquesParisFrance Department of Medical Microbiology and Infectious Diseases Canisius-Wilhelmina HospitalNijmegenThe Netherlands Department of Medical Microbiology RadboudumcNijmegenThe Netherlands Institute of Microbiology eopold-Franzens-University InnsbruckInnsbruckAustria Molecular Mycology Research Laboratory Centre for Infectious Diseases and MicrobiologySydneyMedical School—Westmead HospitalMarie Bashir Institute for Infectious Diseases and BiosecurityThe University of SydneyWestmead Millennium InstituteWestmeadNSWAustralia Basic Pathology Department Federal University of Parana StateCuritibaParanaBrazil Fellowship from Conselho Nacional de Desenvolvimento Cientifico e Tecnologico(CNPq) BrasiliaBrazil Institute of Preventive and Clinical Medicine BratislavaSlovakia Institut für Hygiene und Mikrobiologie Landesklinikum St.Pölten St.PoltenAustria Institute of Medical Microbiology University Hospital EssenEssenGermany Nursing School of Unigranrio Rio de JaneiroBrazil Scientific Institute of Public Health Culture Collect

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Occupational Performance of Women with Chronic Pelvic Pain and the Potential of Coping as an Unassisted Intervention

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International Journal of Clinical Medicine 2018年第9期9卷 737-749页

作者： Omero Benedicto Poli-Neto Raquel Verceze Bortolieiro Júlio Cesar rosa-e-silva Francisco Jose Candido-dos-Reis Antonio Alberto Nogueira Department of Gynecology and Obstetrics Ribeirao Preto Medical SchoolUniversity of Sao PauloRibeirao PretoSPBrazil

Objective: To assess the satisfaction and occupational performance of women with chronic pelvic pain and to discuss unassisted intervention strategies by the occupational therapist for the care of these patients. Methods: A case-control study was conducted on 75 women with chronic pelvic pain and 75 apparently healthy women. The study was approved by the Research Ethics Committee of the institution and all subjects gave written informed consent to participate. Pain intensity was determined using a visual analogue scale and each patient was submitted to psycho-metric assessment using the Patient Health Questionnaire, the Self-Reporting Questionnaire of Psychiatric Screening and the Tampa Scale of Kinesiophofobia in their Brazilian version. The main outcomes regarding occupational performance and satisfaction were evaluated using the Canadian Measure of Occupational Performance. Results: The performance and satisfaction scores of women with chronic pelvic pain were significantly lower than those of healthy women. The presence of pain and kinesophobia was directly and independently correlated with low performance and satisfaction scores regardless of ethnicity, marital status, schooling, or psychometric scores. Conclusion: women with chronic pelvic pain present significant impairment of satisfaction and occupational performance. Coping is a potential unassisted intervention strategy to be applied to this population by occupational therapists.

关键词： Coping Occupational Therapy Performance Resilience Satisfaction

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Liver transplantation:Fifty years of experience

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World Journal of Gastroenterology 2014年第18期20卷 5363-5374页

作者： alice Tung Wan Song Vivian Iida Avelino-silva Rafael Antonio Arruda Pecora Vincenzo Pugliese Luiz Augusto Carneiro D'Albuquerque Edson Abdala Liver and Digestive Organ Transplantation Unit Gastroenterology DepartmentUniversity of Sao Paulo Medical SchoolSao Paulo 05403-900Brazil Infectious Diseases Department University of Sao Paulo Medical SchoolSao Paulo 05403-900Brazil Hepatitis Research Laboratory Infectious Diseases DepartmentUniversity of Sao Paulo Medical SchoolSao Paulo 05403-000Brazil

Since 1963,when the first human liver transplantation(LT)was performed by Thomas Starzl,the world has witnessed 50 years of development in surgical techniques,immunosuppression,organ allocation,donor selection,and the indications and contraindications for *** has led to the mainstream,wellestablished procedure that has saved innumerable lives ***,there are hundreds of liver transplant centres in over 80 *** review aims to describe the main aspects of LT regarding the progressive changes that have occurred over the *** herein review historical aspects since the first experimental studies and the first attempts at human *** also provide an overview of immunosuppressive agents and their potential side effects,the evolution of the indications and contraindications of LT,the evolution of survival according to different time periods,and the evolution of methods of organ allocation.

关键词： Liver transplantation History Survival Indications

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The in Vitro Antioxidant Capacities of Hydroalcoholic Extracts from Roots and Leaves of Smallanthus sonchifolius (Yacon) Do Not Correlate with Their in Vivo Antioxidant Action in Diabetic Rats

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Journal of Biosciences and Medicines 2016年第2期4卷 15-27页

作者： Ana Carla Broetto Biazon Mariana Marques Nogueira Wendt Júlia rosa Moreira Cristiane Vizioli Castro Ghizoni Andreia Assunção Soares Sandra da silva Silveira Anacharis Babeto de Sá-Nakanishi Ciomar Aparecida Bersani Amado rosane Marina Peralta Adelar Bracht Jurandir Fernando Comar Pharmacy Department Faculdades Integrado Campo Mourao Brazil Biochemistry Department University of Maringá Maringá Brazil Pharmacology and Therapeutics Department University of Maringá Maringá Brazil

Leaf and root extracts of Smallanthus sonchifolius (yacon), have antihyper-glycemic activity and antioxidant properties. The present study aims to compare the in vivo hepatic antioxidant activity of hydroalcoholic extracts of yacon leaves and roots in rats with streptozotocin-induced diabetes in terms of their in vitro antioxidant capacity. Rats were treated during 14 days with 1060 mg·Kg-1 root extract or 400 mg·Kg-1 leaf extract. The latter was richer in phenolics and possessed a much higher in vitro antioxidant activity. Both extracts prevented hyperglycemia in diabetic rats. The liver of diabetic rats presented increased levels of protein carbonyls and ROS and decreased activities of antioxidant enzymes. Treatment with both root and leaf extracts restored the protein carbonyl levels to normality. The root extract also restored the ROS levels to normality, but the leaf extract was not effective. The root extract was also more effective in restoring the activity of at least two important antioxidant enzymes (glucose 6-phosphate dehydrogenase and glutathione peroxidase). In terms of the antioxidant load (which was 17 times lower in the root extract treatment), the in vivo action of the root extract was more effective than the leaf extract in reducing the hepatic oxidative stress that accompanies diabetes.

关键词： Smallanthus sonchifolius Yacon Streptozotocin-Induced Diabetes Liver Oxidative Status

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Morbid Obesity: A Review on the Reasons for Impediments to Physical Exercises and Social Activities

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Modern Plastic Surgery 2020年第3期10卷 93-100页

作者： José Humberto Cardoso Resende Mattheus Duarte da Veiga Jardim Brena Thamyres de Andrade Irineu Leydeane rosa Gomes Gontijo Lucas Radi Cruvinel Zeno Augusto de Sousa Neto Rodrigo Gonç alves de Oliveira daniel Soares de Araújo Emídio silva Falcã o Brasileiro UNIFAN Work Coordinator Aparecida de Goi&#226 nia Brazil UNIFAN Academics of Medicine Aparecida de Goi&#226 nia Brazil UNIFAN Research Coordinator Aparecida de Goi&#226 nia Brazil

Morbid obesity is regarded as a disease due to excess body weight, causing a silence of life as a whole and entailing the most varied disabilities for the person, such as: physical, social, psychological, affective, etc. It represents cases of “public health”, thereby involving competent bodies in the development of solutions that encompass various medical specialties and other health fields, in addition to influencing the mind of these people, causing depression that, due to metabolic involvement, can evolve to the death of the individual. The participation of multidisciplinary health focuses on weight loss, freely and spontaneously, or on the indication of bariatric surgery. We know how difficult it is to lose weight. In order to achieve successful procedures, we recommend the “Obese Workshop” or pre- and post-surgical follow-ups close to the patients, with a view to avoiding recurrences or the “accordion effect” (very common), which can interfere with the Body Mass Index (BMI). Everyone, males and females, complained of tiredness and the impossibility of any physical exercise, even the lightest and simplest to be performed, in addition to the fact that they cannot attend gyms and are unable to open a simple door handle.

关键词： Obesity Hypertension Diabetes Bariatric Illness Physical Exercise

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Testosterone Levels and Development of the Penile Spines and Testicular Tissue during the Postnatal Growth in Wistar Rats

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Advances in Sexual Medicine 2013年第3期3卷 1-9页

作者： Marcela Arteaga silva rosa M.Vigueras Villasenor Socorro Retana Marquez Marisela Hernandez Gonzalez Herlinda Bonilla Jaime Xochitl Guzman García Jose Luis Contreras Montiel Department of Biology of Reproduction Metropolitan Autonomous UniversityIztapalapaMexico Laboratory of Reproductive Biology National Institute of PediatricsMexico CityMexico Institute of Neuroscience University of GuadalajaraJaliscoMexico Department of Hydrobiology Metropolitan Autonomous UniversityIztapalapaMexico

Aim of Study: Gonadal hormones exert a profound influence on the development, structure and function of the sexual organs. The testosterone is one of the androgens that plays an essential role in the development of sexual organs in male mammals. Therefore, the present study was undertaken to evaluate the testosterone levels and developmental pattern of the penile spines and seminiferous tubules during early postnatal life of Wistar rats. Methods and Materials: At 7, 14, 21, 28, 35, 42, 49, 56, 63 and 70 days after birth, penile and testicular tissues of male rats were dissected out and fixed for histological study and plasma testosterone levels were determined using high resolution chromatography. Results: An increase in the number of penile follicles, primarily in the distal region of the penis, was observed from postnatal days 14 to 42, followed by a gradual decrease. Penile spines were absent from birth until the first growth peak, which was observed at 42 postnatal days. Both testicular weight and the area of seminiferous tubules showed gradual increases before achieving their highest values at 42 postnatal days. Similarly, a gradual increase in testosterone levels was detected from day 28, with a peak at 42 postnatal days. Conclusions: These data show a temporal association between the development of the penile spines and testicular tissue with gradual increases in testosterone levels. These results may contribute to a better understanding of the behavioral, hormonal and morphological changes underlying sexual maturation in male rats.

关键词： Testosterone Penile Spines Testicular Tissue Postnatal Growth Male Rat

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