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Wide transcriptional investigation unravel novel insights of the on-tree maturation and postharvest ripening of‘Abate Fetel’pear fruit

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Horticulture Research 2019年第1期6卷 1378-1392页

作者： Nicola Busatto Brian Farneti alice tadiello Vicky Oberkofler Antonio Cellini Franco Biasioli Massimo Delledonne Alessandro Cestaro Christos Noutsos Fabrizio Costa Department of Genomics and Biology of Fruit Crops Research and Innovation CentreFondazione Edmund Mach(FEM)Via E.Mach 138010 San Michele all’AdigeItaly Department of Biology University of PadovaVia G.Colombo 335121 PadovaItaly Institute for Biochemistry and Biology Karl-Liebknecht-Str.24-2514476 PotsdamGermany Department of Agricultural and Food Science University of BolognaVia Fanin 4640127 BolognaItaly Department of Food Quality and Nutrition Research and Innovation CentreFondazione Edmund Mach(FEM)Via E.Mach 138010 San Michele all’AdigeItaly Department of Biotecnology University of VeronaStrada le Grazie 15CàVignal 137134 VeronaItaly Unit of Computational Biology Research and Innovation CentreFondazione Edmund Mach(FEM)Via E.Mach 138010 San Michele all’AdigeItaly Biology Department SUNY College at Old WestburyOld WestburyNY 11568USA

To decipher the transcriptomic regulation of the on-tree fruit maturation in pear cv.‘Abate Fetel’,a RNA-seq transcription analysis identified 8939 genes differentially expressed across four harvesting stages.These genes were grouped into 11 SOTA clusters based on their transcriptional pattern,of which three included genes upregulated while the other four were represented by downregulated genes.Fruit ripening was furthermore investigated after 1 month of postharvest cold storage.The most important variation in fruit firmness,production of ethylene and volatile organic compounds were observed after 5 days of shelf-life at room temperature following cold storage.The role of ethylene in controlling the ripening of‘Abate Fetel’pears was furthermore investigated through the application of 1-methylcyclopropene,which efficiently delayed the progression of ripening by reducing fruit softening and repressing both ethylene and volatile production.The physiological response of the interference at the ethylene receptor level was moreover unraveled investigating the expression pattern of 12 candidate genes,initially selected to validate the RNA-seq profile.This analysis confirmed the effective role of the ethylene competitor in downregulating the expression of cell wall(PG)and ethylene-related genes(ACS,ACO,ERS1,and ERS2),as well as inducing one element involved in the auxin signaling pathway(Aux/IAA),highlighting a possible cross-talk between these two hormones.The expression patterns of these six elements suggest their use as molecular toolkit to monitor at molecular level the progression of the fruit on-tree maturation and postharvest ripening.

关键词： furthermore tree insight

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Ethylene-auxin crosstalk regulates postharvest fruit ripening process in apple

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Fruit Research 2021年第1期1卷 116-128页

作者： Nicola Busatto alice tadiello Marco Moretto Brian Farneti Francesca Populin Urska Vrhovsek Mauro Commisso Elisa Sartori Paolo Sonego Franco Biasioli Guglielmo Costa Flavia Guzzo Paolo Fontana Kristof Engelen Fabrizio Costa Research and Innovation Centre Fondazione Edmund Machvia Mach 138098 San Michele all'AdigeTrentoItaly Department of Biotechnology University of VeronaStrada le Grazie 15CàVignal 137134 VeronaItaly Department of Agricultural Science University of BolognaVia Fanin 4640127BolognaItaly Center Agriculture Food Environment C3A University of TrentoVia Mach 138098 San Michele all'AdigeTrentoItaly

The ripening of climacteric fruits,such as apple,is represented by a series of genetically programmed events orchestrated by the action of several hormones.In this study,we investigated the existence of a hormonal crosstalk between ethylene and auxin during the post-harvest ripening of three internationally known apple cultivars:‘Golden Delicious',‘Granny Smith'and‘Fuji'.The normal climacteric ripening was impaired by the exogenous application of 1-methylcyclopropene(1-MCP)that affected the production of ethylene and the physiological behaviour of specific ethylene-related quality traits,such as fruit texture and the production of volatile organic compounds.The application of 1-MCP induced,moreover,a de-novo accumulation of auxin.The RNA-Seq wide-transcriptome analysis evidenced as the competition at the level of the ethylene receptors induced a cultivar-dependent transcriptional re-programming.The DEGs annotation carried out through the KEGG database identified as most genes were assigned to the plant hormone signaling transduction category,and specifically related to auxin and ethylene.The interplay between these two hormones was further assessed through a candidate gene analysis that highlighted a specific activation of GH3 and ILL genes,encoding key steps in the process of the auxin homeostasis mechanism.Our results showed that a compromised ethylene metabolism at the onset of the climacteric ripening in apple can stimulate,in a cultivar-dependent fashion,an initial de-novo synthesis and de-conjugation of auxin as a tentative to restore a normal ripening progression.

关键词： crosstalk cultivar process

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