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A novel Ga(Ⅲ)coordination complex as an efficient sensitizer for enhancing photocatalytic activity of TiO_(2)/rGO nanocomposite

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Journal of Rare Earths 2023年第7期41卷 1004-1013,I0002页

作者： Amir Hossein Cheshme Khavar Neda Khedri Rubén Rizo Juan Miguel Feliu Martínez ali Reza mahjoub Masoumeh Doolabi Ehsan Aghayani Department of Chemistry Farhangian UniversityTehranIran Department of Chemistry Faculty of Basic SciencesTarbiat Modares UniversityTehranIran Instituto de Electroquímica y Departamento de Química Física Universidad de Alicante03080AlicanteSpain Research Center for Environmental Contaminants Abadan University of Medical SciencesAbadanIran Department of Environmental Health Engineering Abadan University of Medical SciencesAbadanIran

Narrow solar-light response range and rapid charges recombination are the main technical barriers in TiO_(2) photocatalysis *** overcome these restrictions,in this work we synthesized a novel binuclear gadolinium(Ⅲ) coordination complex,[Gd_(2)(DPDB)_6(DMF)_6(H_(2)O_()2)](DPDB=[(4-dimethylamino)phenyldiazenyl] benzenesulfonate),which was used as an inorganic sensitizer for boosting the visible light-harvesting and quantum efficiency of TiO_(2) supported-reduced graphene oxide(rGO) *** structure of [Gd_(2)(DPDB)_6(DMF)_6(H_(2)O)_(2)](Gd-CMP) was determined from single-crystal X-ray diffraction data,which indicates three-dimensional(3D) supramolecular architecture through hydrogen bonding and CH…π_(ph) *** prepared nanohybrid(Gd-CMP/TG) reveals significantly enhanced visible-light-induced photocatalytic activity for degradation of acetaminophen(ACT).The complete removal of 10 mg/L ACT is achieved over Gd-CMP/TG,and the corresponding rate constant of ACT degradation of nanohybrid is 40-fold higher than that of bare TiO_(2).In prepared ternary nanohybrid,metal-coordination sensitizer(Gd-CMP) acts as an electron donor,and at the same time,rGO serves as an electron acceptor,and the synergistic effect between them efficiently enhances charges separation and inhibits e/h pair recombination in the hybridized *** radical scavenger tests indicate that the photo-induced O_(2)^(-·) radicals dominate ACT *** the basis of the experimental results and calculated energy of electronic levels,a possible mechanism for the improved photocatalytic activity is discussed.

关键词： Gadolinium Coordination complex Sensitizer Photocatalytic Light-harvesting Rare earths

Characterization and of Ralstonia TAL-Like DNA-Binding Specificities Effectors

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Molecular Plant 2013年第4期6卷 1318-1330页

作者： Lixin Li Ahmed Atef Agnieszka Piatek Zahir ali Marek Piatek Mustapha Aouida Altanbadralt Sharakuu ali mahjoub Guangchao Wang Suhail Khan Nina V. Fedoroff Jian-Kang Zhu Magdy M. Mahfouz Center for Desert Agriculture King Abdullah University of Science and Technology Thuwal 23955-6900 Kingdom of Saudi Arabia Department of Biological Sciences Faculty of Sciences King Abdulaziz University PO Box 80203 Jeddah 21589 Kingdom of Saudi Arabia Imaging Core Laboratory King Abdullah University of Science and Technology Thuwal 23955-6900 Kingdom of Saudi Arabia Department of Horticulture and Landscape Architecture Purdue University West Lafayette IN 47907 USA

Transcription activator-like effectors （TALEs） from Xanthomonas sp. have been used as customizable DNA- binding modules for genome-engineering applications, Ralstonia solanacearum TALE-like proteins （RTLs） exhibit similar structural features to TALEs, including a central DNA-binding domain composed of 35 amino acid-long repeats. Here, we characterize the RTLs and show that they localize in the plant cell nucleus, mediate DNA binding, and might function as transcriptional activators. RTLs have a unique DNA-binding architecture and are enriched in repeat variable di-residues （RVDs）, which determine repeat DNA-binding specificities. We determined the DNA-binding specificities for the RVD sequences ND, HN, NP, and NT. The RVD ND mediates highly specific interactions with C nucleotide, HN interacts spe- cifically with A and G nucleotides, and NP binds to C, A, and G nucleotides. Moreover, we developed a highly efficient repeat assembly approach for engineering RTL effectors. Taken together, our data demonstrate that RTLs are unique DNA-targeting modules that are excellent alternatives to be tailored to bind to user-selected DNA sequences for targeted genomic and epigenomic modifications. These findings will facilitate research concerning RTL molecular biology and RTL roles in the pathogenicity of Ralstonia spp.

关键词： Ralstonia solanacearum genome engineering TAL effectors TALE activators and repressors TALE nucleases (TALENs) targeted genome modifications.

Antioxidant and antimicrobial activities of Padina pavonica and Enteromorpha sp. from the Tunisian Mediterranean coast

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Journal of Coastal Life Medicine 2017年第8期5卷 336-342页

作者： Malek Besbes Hlila Amel Omri Hichri Mohamed ali mahjoub Zine Mighri Maha Mastouri Laboratory of Transmissible Diseases and Biological Active Substances Faculty of PharmacyAvenue Avicenne5000MonastirUniversity of MonastirMonastirTunisia Laboratory of Heterocyclic Chemistry Natural Products and ReactivityTeam:Medicinal Chemistry and Natural ProductsFaculty of Sciences ofMonastirUniversity of MonastirMonastirTunisia

Objective: To examine the antioxidant and the antimicrobial activities of the marine seaweeds Padina pavonica (P. pavonica) and Enteromorpha sp. from the Tunisian Mediterranean coast. Methods: The acetone and water were used for algae extraction to envisage the antimicrobial activity versus Gram-negative (Escherichia coli and Pseudomonas aeruginosa), Gram-positive bacteria (Staphylococcus aureus and Enterococcus faecalis) and against four Candida. The microdilution method was used to evaluate this activity. In vitro, total phenolic content and the antioxidant activity including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and 2,2'-azino-bis-(3-ethylbenthiazoline)-6-sulfonic acid (ABTS) were also studied. Results: The highest amount of phenolic compound was found in the P. pavonica acetonic extract ((90.61 ± 0.11) mg catechin equivalent/g extract)This brown algae sample demonstrated greater DPPH and ABTS radical scavenging ability potential in comparison to other green seaweed, Enteromorpha sp. The maximum antimicrobial activity was shown by the P. pavonica acetonic extract against all the pathogenic strains tested (minimum inhibitory concentrations = minimum inhibitory bactericidal = minimum inhibitory fungicidal concentrations = 0.04 mg/mL). Those activities might be due to phenolic substances present in this fraction. Conclusions: The present results highlight the possible use of P. pavonica as source of antioxidant and antimicrobial compounds.

关键词： Padina pavonica Enteromorpha sp. Antioxidant Antimicrobial

新烟碱类杀虫剂对麦扁盾蝽若虫和成虫的毒效(英文)

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昆虫学报 2011年第8期54卷 938-942页

作者： ali Akbar JAFARPOUR Aziz SHEIKHI GARJAN Sohrab IMANI Seyed Mahdi mahjoub Mohammad MAHMOUDVAND Islamic Azad University Science & Research BranchTehran 19395/1445Iran Iranian Research Institute of Plant Protection Tehran 19395/1445Iran Section of Plant Protection Agricultural Research CentreKermanshahIran Department of Plant Protection Khorramabad BranchIslamic Azad UniversityKhorramabadIran

在伊朗,麦扁盾蝽Eurygaster integriceps是小麦和大麦上的主要害虫。新烟碱类杀虫剂是一类具有较强活性的杀虫剂,对刺吸式口器害虫具有良好的效果。本研究应用触杀和熏蒸两种生测方法测定了4种新烟碱类杀虫剂(噻虫嗪、吡虫啉、呋虫胺和... 详细信息

在伊朗,麦扁盾蝽Eurygaster integriceps是小麦和大麦上的主要害虫。新烟碱类杀虫剂是一类具有较强活性的杀虫剂,对刺吸式口器害虫具有良好的效果。本研究应用触杀和熏蒸两种生测方法测定了4种新烟碱类杀虫剂(噻虫嗪、吡虫啉、呋虫胺和啶虫咪)对麦扁盾蝽3龄若虫和成虫的毒效。触杀法生测结果显示,各杀虫剂对麦扁盾蝽没有强烈的触杀活性,但是在高浓度下(5000mg/La.i.),可见较高的死亡率。熏蒸法生测结果显示,所有杀虫剂对麦扁盾蝽的若虫和成虫均有较好的毒性。结果提示应用熏蒸方法可能是治理这一重要害虫的新方法。

关键词：麦扁盾蝽新烟碱类杀虫剂毒性触杀活性熏蒸刺吸式口器害虫

Introducing a Rapid and Safe Method for Myeloperoxidase Staining

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Open Journal of Pathology 2015年第2期5卷 38-41页

作者： Fatemeh E. mahjoub Fahimeh Firouzjaie Karder Issa Jahanzad Saghi Vaziri Ramezan ali Sharifian Zahra Farahani Pathology Maternal Fetal and Neonatal Research Center Tehran University of Medical Sciences Tehran Iran Imam Khomeini Hospital Complex Central Laboratory and Cancer Institute Pathology Laboratory Tehran University of Medical Sciences Tehran Iran Valiasr Hospital Central Laboratory Tehran University of Medical Sciences Tehran Iran Imam Khomeini Hospital Complex Cancer Institute Tehran University of Medical Sciences Tehran Iran Hematology Oncology Imam Khomeini Hospital Complex Valiasr Hospital Tehran University of Medical Sciences Tehran Iran

Background: Myeloperoxidase staining is used to differentiate leukemias since several decades. Despite implementation of flow cytometric, cytogenetic and molecular techniques for identification of leukemic blasts, histochemical stains such as myeloperoxidase stain are persistently used for better classification of leukemias. The myeloperoxidase staining is a time consuming and hazardous procedure. The present report describes a sensitive, rapid and easy method for assessment of peroxidase activity. Materials and Methods: Bone marrow aspiration slides were stained with Dako product: Code number: K3467 containing DAB chromogen (3,3-diaminobenzidine in chromogen solution) and substrate buffer (Imidasole-HCL buffer, PH 7.5 containing hydrogen peroxide and an anti microbial agent) in a rapid procedure taking only ten minutes time. The staining needs no material preparation steps. Neutrophils in the slide are taken as positive control or another normal smear was costained to be used as control. All cases were followed up with flow cytometry and cytogenetic studies. Result: The reaction product of this stain is brown and granular. Promyelocytes and myelocytes are the most strongly staining cells with positive (primary) granules. Lymphoblasts are negative. The result of classification of leukemias with this technique was in concordance with flow cytometric immunophenotyping. Discussion: Many practical techniques have been described using benzidine as an indicator for myeloperoxidase staining. Benzidine is a carcinogenic material and its usage is severely restricted in laboratory. Formerly we prepared requisite materials for myeloperoxidase staining by hazardous ways (boiling), but we decided to apply ready to use 3,3-diaminobenzidine (DAB), which is used in final step of immunohistochemistry stains. Conclusion: Use of 3,3-diaminobenzidine (DAB) is highly recommended for myeloperoxidase staining, while the result is extraordinary and fully compatible with flow cytometry and

关键词： Myeloperoxidase Staining DAB 3 3-Diaminobenzidine Safe and Rapid Method

Urosepsis among Sudanese Patients: A Paradigm from Limited Resources Country

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Advances in Microbiology 2022年第3期12卷 109-120页

作者： Salma Omer Ibrahim Mohammed Elimam Sami mahjoub Taha Sanaa Mohammed Yousif Hajir Mohammed Hussein Omer Mirghani Yousif Seitelbanat Yassin ali Elnaiem Yousif Abdelhameed Mohammed Omer Abu Elhasan Adama Dawoud Abakar Elhadi Abdalla Ahmed Department of Medical Microbiology Faculty of Medical Lab Sciences University of Gezira Wad Medani Sudan Faculty of Medicine University of Gezira Wad Medani Sudan National Blood Bank Ministry of Health Khartoum Sudan Clinical Pharmacy and Pharmacy Practice Faculty of Pharmacy University of Gezira Wad Medani Sudan Department of Pharmaceutics Faculty of Pharmacy University of Gezira Wad Medani Sudan Resident at Gezira Hospital for Renal Disease and Surgery Urology (GHRDS) Wad Medani Sudan Cancer National Institute University of Gezira Wad Medani Sudan Wad Madani College for Medical Science and Technology Wad Medani Sudan Department of Medical Parasitology Faculty of Medical Laboratory Sciences University of Gezira Wad Medani Sudan

Background: Urosepsis is life-threatening sepsis that leads to organ dysfunction and results from a defective response to a urinary tract infection;the major precipitating is obstructive uropathy in the upper or lower urinary tract (UT). The magnitude and burden of bacteria that caused uropathy were reported to increase annually. In 30% of all septic patients who were diagnosed with urosepsis, 1.5% of them were found in urology and a quarter due to hospital-acquired urinary tract infections (HAUTIs). This study aims to determine the clinical pattern and the frequency of commonly used antibiotics against bacteria associated with urosepsis among Sudanese patients. Methods: This was a cross sectional laboratory-based study, study subjects were recruited from patients attended to Gezira Hospital for Renal diseases and surgery (GHRDS) and was diagnosed, on clinical and laboratory basis, to have urosepsis. Hundred (n = 100) urine samples were collected and inoculated on cysteine lactose electrolyte deficient agar (CLED) media and identify using the suitable biochemical test and performed antimicrobial susceptibility testing (AST) by Kirby Bauer disc diffusion technique for selected antimicrobial agents, according to clinical laboratory standard institute (CLSI) guidelines. Results: Amongst urosepsis infection the frequency of E. coli, S. aureus, Proteus mirabilis, Klebseilla pneumonia and Pseudomonas aeruginosa were (37%, 21%, 10%, 6%, 4% respectively). Resistance to commonly prescribed antibiotics was high, ranging from 17% for meropenem to 100% for cefepime. P. aeruginosa was multidrug resistant compared with other isolates. Conclusions: There was high rate of antibiotic resistance against the common causes of urosepsis in GHRDS, and this reflects the importance of culture and sensitivity test and necessitates adoption of guidelines for selection of suitable antibiotic.

关键词： Urosepsis Antimicrobial Susceptibility Gezira Hospital for Renal Diseases and Surgery Sudan

Rental Mucinous Tubular and Spindle Cell Carcinoma:Case Report

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Journal of Oncology Research 2021年第1期3卷 28-30页

作者： Mosab Abdalla ali Alzubier Abd Elmouniem ali Elgasim Sami mahjoub Taha Assistant Professor of Urology MBBSMRCSEF urologyGadarif UniversityFaculty of MedicineSudan.Temporally Assistant Professor of UrologyPrince Sattam ibn Abdulaziz UniversityFaculty of MedicineConsultant Urologist Prince Sattam University Hospital.Al kharjKSA Consultant Urologist Ibn sina HospitalSudan Professor of Urology University of GeziraFaculty of MedicineSudan

Mucinous tubular and spindle cell carcinoma(MTSCC)of the kidney is an uncommon recently recognized renal cell *** reported A 60 year's old man who presented with right flank pain,abdominal swelling and one attack of *** intraoperative finding was a huge cystic swelling arising from the right kidney occupying almost all the abdominal cavity displacing the bowel to the left side of the *** was no ascites or evidences of *** radical nephrectomy was *** the diagnosis of renal MTSCC was *** condition of the patient was improved and one year prognosis was *** our knowledge this is the first reported case of MTSCC in Sudan,and the outcome of treatment was satisfactory.

关键词： Mucinous tubular and spindle cell carcinoma Renal tumour Sudan

Congenital Microvillous Atrophy, Report of Two Consecutive Siblings with Complete Histologic, Immunohistochemical and Detailed Electron Microscopic Studies, First Report from Iran

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Open Journal of Pathology 2014年第2期4卷 64-67页

作者： Fatemeh Elham mahjoub Seyedeh Nakisa Niknejad Mahnaz Sadeghian Afshin Abdirad Mehri Najafi Sani Issa Jahanzad Elham Talachian 1Maternal Fetal and Neonatal Research Center Tehran University of Medical Sciences Tehran Iran 2Imam Khomeini Hospital Complex Affiliated with Tehran University of Medical Sciences Tehran Iran ali-Asghar Children Hospital Children Hospital Affiliated with Iran University of Medical Sciences Tehran Iran Markaze Tebbi Koodakan Children Hospital Affiliated with Tehran University of Medical Sciences Tehran Iran Maternal Fetal and Neonatal Research Center Tehran University of Medical Sciences Tehran Iran Tehran University of Medical Sciences Tehran Iran

Introduction: Microvillous inclusion disease (MVD) or microvillous atrophy disorder is a congenital disorder of the small intestinal epithelial cells that presents with persistent and severe diarrhea and it is characterized by enterocytes abnormalities [1] 08D0C9EA79F9BACE118C8200AA004BA90B02000000080000000E0000005F005200650066003300380035003800380033003700360030000000 . For these children, prognosis is generally poor due to metabolic acidosis with poor compensation. To our experiment, this disease is very rare in Iran and it is yet unreported, so we decided to report two consecutive siblings with the same disease from Iran. Report of Cases: Two siblings were born to healthy parents. Parents were cousins. Both siblings were hospitalized due to severe diarrhea starting shortly after breast feeding. The frequency of diarrhea in both cases was 10 to 17 times per day and their stools were loose and green. Histological studies of both siblings revealed duodenal mucosa with complete flattening of villi (total villous atrophy). Superficial lining cells showed atrophy. Crypts showed no hyperplasia, however it showed distortion and difference in size. By PAS staining and CD10 staining, a poorly developed brush border and typical inclusions were seen in apical boarder of enterocytes. Electron microscopy was performed for the second case and showed microvillous involution and inclusions in the apical part of the epithelial cells. Discussion: Microvillous congenital atrophy is a rare congenital disorder. Due to rareness of congenital microvillous atrophy (CMA), it is crucial to distinguish it from other diseases with persistent and severe diarrhea as soon as possible.

关键词： Congenital Microvillous Atrophy Pediatric Diarrhea Histopathological Findings Electron Microsopy