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Ethyl Methane Sulphonate (EMS) Induced Mutagenesis in Malaysian Rice (cv. MR219) for Lethal Dose Determination

American Journal of Plant Sciences 2012年第12期3卷 1661-1665页

作者： ali benjavad talebi Amin benjavad talebi Behzad Shahrokhifar 2De- partment of Computer Sciences Faculty of Science and Information Technology National University of Malaysia (UKM) Bangi Malaysia Department of Plant Biotechnology National Institute of Genetic Engineering and Biotechnology (NIGEB) Tehran Iran Department of Weed Science Shoushtar Branch Islamic Azad University Shoushtar Iran

Chemical and physical mutagenesis has been used to increase genetic variability in crop plants. More than 430 new varieties have been derived as mutants of rice (Oryza sativa L.) via the application of different mutagenic agents. Chemical mutagens such as ethyl methane sulphonate (EMS), diepoxybutane-derived (DEB), sodium azide and irradiation (Gamma rays, X-rays and fast neutrons) have been widely used to induce a large number of functional variations in rice and others crops. Among chemical mutagens, the alkylating agent, ethyl methane sulfonate (EMS) is the most commonly used in plants as it causes a high frequency of nucleotide substitutions, as detected in different genomes. In this study, seeds of potential genotype of the popular variety, (Oryza sativa L. spp. Indica cv. MR219) were treated with EMS at concentrations of 0.25%, 0.50%, 0.75%, 1%, 1.25%, 1.5% and 2%. Sensitivity to EMS was determined by various measurements on the M1 generation. As concentration of applied EMS increased, will decrease in germination, seedling height, root length and emergence under field conditions was observed in M1 generation as compared to the non-treatment control. Plant height and root length also decreased with increases in EMS mutagenesis in an approximately linear fashion. The LD25 and LD50 values were observed based on growth reduction of seedlings after EMS treatment with 0.25% and 0.50% on the rice variety (Oryza sativa L. spp. Indica cv. MR219).

关键词： EMS Lethal Dose Chemical Mutagenesis Ethyl-Methane Sulfonate Oryza Sativa Indica cv. MR219

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