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Plasticity of wheat seedling responses to K^(+) deficiency highlighted by integrated phenotyping of roots and root hairs over the whole root system

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Stress Biology 2023年第1期3卷 69-83页

作者： Ikram Madani Jean-Benoit Peltier Martin Boeglin Hervé Sentenac Anne-aliénor Véry Institut des Sciences des Plantes de Montpellier UMR 5004 CNRS-386 INRAE-Université Montpellier-Institut AgroCampus SupAgro-INRAE Bat 7Place VialaMontpellier 34060 Cedex 2France

The availability in the soil of potassium(K^(+)),a poorly mobile macronutrient required in large quantities for plant growth,is generally suboptimal for crop production in the absence of fertilization,making improvement of the ability of crops to adapt to K^(+)deficiency stress a major *** the uptake capacity of the root system is among the main strategies to achieve this ***,we report an integrative approach to examine the effect of K^(+)deficiency on the development of young plant entire root system,including root hairs which are known to provide a significant contribution to the uptake of poorly mobile nutrients such as K^(+),in two genetically distant wheat varieties.A rhizobox-type methodology was developed to obtain highly-resolved images of root and root hairs,allowing to describe global root and root hair traits over the whole root system via image analysis *** two wheat varieties responded differently to the K^(+)shortage:Escandia,a wheat ancestor,reduced shoot biomass in condition of K^(+)shortage and substantially increased the surface area of its root system,specifically by increasing the total root hair *** Zenati,a landrace,conversely appeared unresponsive to the K^(+)shortage but was shown to constitutively express,independently of the external K^(+)availability,favorable traits to cope with reduced K^(+)availability,among which a high total root hair ***,valuable information on root system adaptation to K^(+)deficiency was provided by global analyses including root hairs,which should also be relevant for other nutrient stresses.

关键词： K^(+)deficiency Root hair Root system Phenotyping Wheat Rhizobox Root plasticity

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甜瓜钾离子通道基因MIRK受外界Na+调控的分子机制

甜瓜钾离子通道基因MIRK受外界Na+调控的分子机制

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中国园艺学会第八届青年学术讨论会暨现代园艺论坛

作者：张屹东邓扬悟 Anne-aliénor Véry Herré Sentenac 黄丹枫上海交通大学农业与生物学院

从网纹甜瓜(Cucumis melo L.)中克隆出的第一个钾离子通道基因MIRK(Melon Inward Rectifying Kchannel,GenBank accession number:DQ116940),通过对其进行电生理和生物信息学分析,发现该通道具有一些明显区别于其他已经克隆出来的植物... 详细信息

从网纹甜瓜(Cucumis melo L.)中克隆出的第一个钾离子通道基因MIRK(Melon Inward Rectifying Kchannel,GenBank accession number:DQ116940),通过对其进行电生理和生物信息学分析,发现该通道具有一些明显区别于其他已经克隆出来的植物钾离子通道的特征:①通过膜片钳研究该钾离子通道的电生理特性,发现该钾离子通道蛋白内向钾电流信号受外界钠离子强烈抑制(调节)。当外界Na浓度从0 mmol·L提高到10 mmol·L时,外界10 mmol·L K浓度激发的钾离子内向电流信号降低约一倍。电压钳结果同样显示,当外界Na浓度继续提高至100 mmol·L时,外界10 mmol·LK浓度激发的钾离子内向正态化尾电流信号降低到近乎为零;②通过将MIRK通道蛋白序列与从其他植物中克隆出来的Shaker家族钾离子通道蛋白序列进行比对,发现位于MIRK通道蛋白通道孔保守区内有两个位点的氨基酸残基(L252和Q234)与其他钾离子通道蛋白有显著区别。其他植物Shaker家族钾离子通道蛋白在252位均为R(精氨酸,碱性带正电荷的氨基酸残基),而MIRK在该位点为中性的不带电荷的L(亮氨酸);同样在234位点只有MIRK和其他3个Shaker家族钾离子通道为不带正电荷的氨基酸残基Q(谷氨酰胺)。基于这两个发现,我们初步认为这两个位点与该通道电生理信号受钠离子抑制(调控)有关。下一步将通过定点突变并结合膜片钳技术对定点突变结果进行验证,探索该甜瓜钾离子通道MIRK受外界钠离子抑制的分子机制,以期为解决作物盐胁迫下钾营养的高效率运输提供一定的理论基础。

关键词：甜瓜钾离子通道 MIRK 调控定点突变

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Tendon Transfer for Foot Drop: A Simple Procedure with Effective Outcome Local Experience

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Open Journal of Modern Neurosurgery 2019年第3期9卷 258-268页

作者： Hesham El Sobky Nabil ali Amr Farid Khalil Department of Neurosurgery Mansoura University Mansoura Egypt

Introduction:?Patients presented with old neglected common peroneal nerve injuries, failed 2ry repair, compression neuropathies not improved after decompression and closed injuries with no improvement on conservative measures which are still unsolved problems. Poor recovery of this nerve after repair or decompression gives great importance to tendon transfer in these situations. Material and Methods: This study was conducted in Neurosurgery Department of Mansoura University from 2015 to 2018 on twenty-six patients (16 males and 10 females). Their age ranged from 6 - 58 years. All of them presented with persistent foot drop at least for one year after injury with no signs of improvement clinically or in electrophysiological studies. All patients underwent tibialis posterior tendon transfer through the interosseous membrane to the tendons of the leg anterior compartment. All of them were put in a plaster cast for four weeks postoperative. A physiotherapeutic program was applied for all patients as preoperative preparation and postoperative rehabilitation. Results: Twenty-two patients showed satisfactory results, three patients fair improvement and one patient no improvement. Two patients had wound infection and there was no mortality in this study. Conclusion:?Tendon transfer is an effective valid solution to be considered in the management of foot drop in these patients.

关键词： Foot Drop Tibialis Posterior Transfer

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Factors Influencing the Photocatalytic Degradation of Reactive Yellow 145 by TiO<sub>2</sub>-Coated Non-Woven Fibers

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American Journal of Analytical Chemistry 2014年第8期5卷 445-454页

作者： Said Alahiane Samir Qourzal Mahmoud El Ouardi Abdelhadi Abaamrane ali Assabbane Physical Chemistry Laboratory Photocatalysis and Environment Team Department of Chemistry Faculty of Science Ibn Zohr University Agadir Morocco

The photocatalytic degradation of the synthetic textile dye Reactive Yellow 145 (ry 145) in aqueous solution, using TiO2 coated non-woven fibers as photocatalyst, under UV-lamp irradiation, was studied. The effects of the operational parameters such as initial dye concentration, pH, addition of oxidant hydrogen peroxide and addition of ethanol on the reaction rate were investigated. The effect of some inorganic ions such as and , commonly present in real effluents, on the photodegradation of ry 145 was also examined. The experimental results showed that the photocatalytic degradation rate was favoured by a high concentration of solution in respect to Langmuir-Hinshelwood model. The maximum rate of complete decolorization of ry 145 was observed in the acidic medium at pH 3. The presence of and anions led to an increase of the effectiveness of the photocatalytic degradation. However, the presence of and anions decreased differently the photodegradation reaction rate. TiO2/UV process was proved to be capable of the complete degradation of the ry 145.

关键词： Photocatalytic Degradation Immobilized TiO2 Reactive Yellow 145 Water Treatment

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