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Optimization Techniques and Development of Neural Models Applied in Biosurfactant Production by <i>Bacillus subtilis</i>Using Alternative Substrates

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Advances in Bioscience and Biotechnology 2017年第10期8卷 343-360页

作者： Juliana Ferrari Ferreira Secato Brunno Ferreira dos Santos alexandre nunes ponezi Elias Basile Tambourgi School of Chemical Engineering (FEQ) Department of Chemical Systems Engineering (DESQ) University of Campinas Campinas Brazil Department of Chemical and Materials Engineering (DEQM) Pontifical Catholic University of Rio de Janeiro Rio de Janeiro Brazil Research Center for Chemistry Biology and Agriculture (CPQBA) University of Campinas Campinas Brazil

Bacillus subtilis was investigated as production of biosurfactant using a combination based on waste of candy industry and glycerol from biodiesel production process as only substrate. The experimental design chosen for optimization by response surface methodology was a central composite rotatable design (CCRD) and dry weight (DW) and crude biosurfactant (CB) concentrations were selected as responses in analysis. Two techniques were implemented response surface methodology (RSM) and artificial neural network (ANN). First challenge of study was to assess the effects of the interactions between variables and reach optimum values. With the CCRD results, RSM and ANN models were developed, optimizing the production of biosurfactant. The correlation coefficients (R2) of RSM models explained 88% for DW and 73% for CB of the interactions among substrate concentrations, while ANN models explained 99% for DW and 98% for CB, demonstrating that developed ANN models were more accurate and consistent in predicting optimized conditions than RSM model. The maximum DW and CB produced in the optimum conditions were 25.60 ± 5.0 g/L and 668 ± 40 mg/L, respectively. The crude biosurfactant also showed applications in cases of oil spreading in water due to clear zone produced in Petri dishes assays.

关键词： Biosurfactant Bacillus subtilis Response Surface Methodology Artificial Neural Network Oil Spreading Waste Management

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Structural and optical properties of pillared Eu^(3+)-containing layered double hydroxides intercalated by 2-to 12-carbon aliphatic dicarboxylates

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Journal of Rare Earths 2022年第2期40卷 260-267,I0003页

作者： alexandre Candido Teixeira Ivan Guide nunes Silva Alysson Ferreira Morais Danilo Mustafa Physics Institute.University of Sao Paulo 05508-090Sao PauloSPBrazil

While the versatile composition of layered double hydroxides(LDHs)allows the introduction of almost any polyvalent cation in their structure,the exchangeable negatively charged ions intercalated between the plates increase their vast range of *** we report on the preparation and optical properties of pillared Eu^(3+)-substituted ZnAl LDHs intercalated by aliphatic dicarboxylates:-OOC-(CH_(2))_(n-2)-COO-.The basal distance in these materials is dependent on the size and packing of the intercalated *** varying the number(n)of carbon atoms in the aliphatic chain from 2 to 12,the interlayer gallery of these ZnAlEu LDHs is considerably expanded from 0.9 to 2.1 *** the interlayer gallery,the aliphatic dicarboxylates form a monolayer,with the aliphatic chain inclined by an angleα≈63°with the hydroxide *** carbon atom in the COO-group is 0.43 nm far from the metal plane,showing that these carboxylates are not grafted in the brucite-like *** LDHs are thermally stable up to around 150℃,after which dehydroxylation of the hydroxide layers is *** Judd-Ofelt intensity parameters were obtained and compared to the Eu^(3+)-containing complexes with the same ligand series indicating a more symmetrical and less polarizable chemical environment around the rare earth ion.

关键词： Europium Layered double hydroxides Carboxylate ligands Luminescence Rare earths

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Rescuing agroforestry as strategy for agriculture in Southern Brazil

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Journal of Forestry Research 2016年第4期27卷 739-746页

作者： alexandre Siminski Karine Louise dos Santos Juliano Gil nunes Wendt Universidade Federal de Santa Catarina Centro de Curitibanos

Knowledge about agricultural ecosystems, as well as about ecological and social interactions that occur in an agricultural context, is essential to promote a balanced environment for production. Incorporating the forest landscape into farming systems has promoted the development of integrated production systems such as agroforestry. However, many aspects of the relationship between farming and the forest landscape have changed since the modernization of agriculture introduced by the Green Revolution. The objectives of this review are：（1） to contribute to the understanding of the changes in cultivation strategies and in the dynamics of agricultural systems,particularly in the context of farming in Santa Catarina State, Brazil;（2） to contribute to the understanding of agroforestry systems as a strategy to reintroduce the element of forestry into agricultural production systems; and（3） to compile a list of forest plant species that could be used in agroforestry. The approach adopted in this manuscript seeks to highlight the potential of agroforestry systems to conserve natural resources and thereby to support agricultural development by connecting environmental conservation with income for farmers.

关键词： agriculture agricultural farming Brazil landscape cultivation modernization highlight income compile

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Liver transplantation as an alternative for the treatment of perihilar cholangiocarcinoma: A critical review

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Hepatobiliary & Pancreatic Diseases International 2024年第2期23卷 139-145页

作者： Wellington Andraus Francisco Tustumi alexandre Chagas Santana Rafael Soares nunes Pinheiro Daniel Reis Waisberg Liliana Ducatti Lopes Rubens Macedo Arantes Vinicius Rocha Santos Rodrigo Bronze de Martino Luiz Augusto Carneiro D’Albuquerque Department of Gastroenterology Transplantation UnitUniversidade de São PauloSão PauloBrazil

Background:Perihilar cholangiocarcinoma(phCCC)is a dismal *** is no consensus regard-ing the best treatment for patients with unresectable *** present review aimed to gather the current pieces of evidence for liver transplantation and liver resection as a treatment for phCCC and to build better guidance for clinical *** sources:The search was conducted in PubMed,Embase,Cochrane,and *** related references were searched *** criteria were:reports in English or Portuguese literature that a)patients with confirmed diagnosis of phCCC;b)patients treated with a curative intent;c)patients with the outcomes of liver resection and liver *** reports,reviews,letters,editorials,conference abstracts and papers with full-text unavailability were excluded from the ***:Most of the current literature is based on observational retrospective studies with low grades of *** resection has better long-term outcomes than systemic chemotherapy or palliation ther-apy and liver transplantation is a good alternative for selected patients with unresectable *** candidates for resection or transplantation should be medically fit and free of intrahepatic or extrahep-atic *** a general rule,patients presenting with a tumor having a longitudinal size>3 cm or extending below the cystic duct,lymph node disease,confirmed extrahepatic dissemination;intraoper-atively diagnosed metastatic disease;a history of other malignancies within the last five years,and did not complete chemoradiation regimen and were medically unfit should not be considered for *** of these criteria should be individually *** transplantation or resection should only be considered in highly experienced hepatobiliary centers,and any decision-making must be based on a multidisciplinary ***:phCCC is a complex condition with high *** therapies,including hepatec-tomy and liv

关键词： Liver transplantation Cholangiocarcinoma Liver neoplasms

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Giardia infection:Protein-losing enteropathy in an adult with immunodeficiency

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World Journal of Gastroenterology 2012年第19期18卷 2430-2433页

作者： alexandre Khodr Furtado Virginia Lucia Ribeiro Cabral Thiago nunes Santos Eli Mansour Cristiane Kibune Nagasako Sonia Leticia Lorena Rogerio Antunes Pereira-Filho Faculdade de Ciências Médicas Universidade de Campinas-UnicampRua Tessália Vieira de Camargo126Cidade Universitária "Zeferino Vaz"13083-887 CampinasBrazil

The case of a 52-year-old woman with a past history of thymoma resection who presented with chronic diarrhea and generalized edema is the focal point of this article.A diagnosis of Giardia lamblia infection was established,which was complicated by protein-losing enteropathy and severely low serum protein level in a patient with no urinary protein loss and normal liver *** anti-helmintic treatment,there was recovery from hypoalbuminemia,though immunoglobulins persisted at low serum levels leading to the hypothesis of an immune system ***'s syndrome is a rare cause of immunodeficiency characterized by the association of hypogammaglobulinemia and *** primary immune disorder may be complicated by severe infectious diarrhea secondary to disabled humoral and cellular immune *** is the first description in the literature of an adult patient with an immunodeficiency syndrome who presented with protein-losing enteropathy secondary to giardiasis.

关键词： Chronic diarrhea Giardiasis Protein-losingenteropathy Immunodeficiency

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Fluid and electrolyte overload in critically ill patients: An overview

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World Journal of Critical Care Medicine 2015年第2期4卷 116-129页

作者： Bruno Adler Maccagnan Pinheiro Besen André Luiz nunes Gobatto Lívia Maria Garcia Melro alexandre Toledo Maciel Marcelo Park Intensive Care Unit Department of Medical Emergencies Hospital das Clínicas University of Sao Paulo Medical School Imed Research Group Intensive Care Unit Hospital Sao Camilo Pompéia

Fluids are considered the cornerstone of therapy for many shock states, particularly states that are associated with relative or absolute hypovolemia. Fluids are also commonly used for many other purposes, such as renalprotection from endogenous and exogenous substances, for the safe dilution of medications and as "maintenance" fluids. However, a large amount of evidence from the last decade has shown that fluids can have deleterious effects on several organ functions, both from excessive amounts of fluids and from their non-physiological electrolyte composition. Additionally, fluid prescription is more common in patients with systemic inflammatory response syndrome whose kidneys may have impaired mechanisms of electrolyte and free water excretion. These processes have been studied as separate entities(hypernatremia, hyperchloremic acidosis and progressive fluid accumulation) leading to worse outcomes in many clinical scenarios, including but not limited to acute kidney injury, worsening respiratory function, higher mortality and higher hospital and intensive care unit lengthof-stays. In this review, we synthesize this evidence and describe this phenomenon as fluid and electrolyte overload with potentially deleterious effects. Finally, we propose a strategy to safely use fluids and thereafter wean patients from fluids, along with other caveats to be considered when dealing with fluids in the intensive care unit.

关键词： Fluid therapy Critically Ill Oliguria Waterelectrolyte balance Central venous pressure Resuscitation Acute kidney injury Diuretics Multiple organ dysfunction syndrome Systemic inflammatory response syndrome

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Combined Selection in Backcross Population of Papaya(Carica papaya L.)by the Mixed Model Methodology

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American Journal of Plant Sciences 2014年第20期5卷 2973-2983页

作者： Helaine Christine Cancela Ramos Messias Gonzaga Pereira alexandre Pio Viana Lucas nunes da Luz Deisy Lucia Cardoso Geraldo Antonio Ferreguetti Laboratory of Plant Breeding Centre for Agricultural Sciences and TechnologyNorth Fluminense State University(UENF)Campos dos GoytacazesBrazil Caliman Agrícola S.A. LinharesBrazil

The selection of superior genotypes based on the simultaneous response to several characteristics of agronomic importance is a key strategy to overcome the scarcity of available varieties of papaya. This study aimed to apply the combined selection by using distinct selection indexes based on both the genetic values obtained by the REML/BLUP methodology and the real measured values to select agronomically superior genotypes of papaya within backcross progenies. The combined selection was carried out based on genetic and phenotypic values, original and standardized, multiplied by the agronomic weights. The results of the analysis of genetic parameters indicate that the evaluated progenies have expressive genetic variability for the considered traits, and that there are real possibilities of genetic progress with the selection. Among the analyzed indexes, the one based on standardized genetic value presented greater consistency in the ranking of genetic material, demonstrating the advantage of data standardization. Five progenies belonging to the BC1 generation, and five to the BC3 generation were selected using this index. A total of 27 plants ag-ronomically superior were selected within the top five progenies and recommended for generation advance, 23 being selected by combined selection and 4 using the direct selection for the four mainly characters in papaya breeding program: production, pulp and fruit firmness and soluble solids. Beyond the selection of superior genotypes for the development of future inbred lines, this study also allowed defining the best strategy to apply the combined selection in papaya using pre-dicted breeding values obtained by BLUP. This strategy may allow higher accuracy in the selection process, thus increasing the chances of success of the breeding programs.

关键词： Papaya Tree Backcross Selection Index Mixed Model REML/BLUP

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Assessment of Acute Episodes in Chronic Stable Angina: A Clinical-Laboratory Approach to the Use of the Coronary Vasodilator Propatyl Nitrate

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World Journal of Cardiovascular Diseases 2016年第7期6卷 246-252页

作者： Gerson Goldwasser Renato Kaufman Carlos Pereira nunes alexandre Hid Stephanie Wrobel Goldberg Alessandra Santos Lisa Oliveira Adenilson de Souza da Fonseca Mauro Geller Souza Marques Medical School Rio de Janeiro Brazil Universidade Estadual do Rio de Janeiro Rio de Janeiro Brazil UNIFESO Medical School Teresópolis Brazil Washington University School of Medicine St. Louis MO USA

Background/Objectives: Propatyl nitrate is a coronary vasodilator with immediate and prolonged action, indicated in the treatment and prevention of acute angina pectoris episodes. Methods: This was an open, self-paired comparative study performed at UNIFESO Medical School evaluating the clinical and laboratory results of treatment with propatyl nitrate in patients with chronic stable angina pectoris. Subjects received 10 mg of propatyl nitrate, at the dose of three sublingual tablets per day, to be taken at 8:00 A.M., 2:00 P.M., and 8:00 P.M. Subjects returned to the study center after 15 days of treatment for Visit 2 assessments, and at the end of the 30-day treatment period (Visit 3). Results: A total of 200 subjects were included in the study. There was a statistically significant reduction in blood pressure (p < 0.0001) and heart rate (p = 0.0001), but no change in respiratory rate (p = 0.23). Laboratory results did not vary throughout the treatment period. There was no significant change from pretreatment in the SAQ Physical Limitation scale (p = 0.7415). The Angina Stability, Angina Frequency, and Treatment Satisfaction, and Quality of Life scales showed a significant improvement from pretreatment (p < 0.0001). Adverse events were observed among 41 subjects at Visit 2 and 35 subjects at Visit 3. Conclusions: Propatyl nitrate was safe and effective in treating chronic stable angina pectoris over the course of the 30-day treatment period. Treatment with propatyl nitrate increased angina stability and reduced angina frequency while increasing treatment satisfaction and quality of life in the patient population evaluated.

关键词： Propatyl Nitrate Stable Chronic Angina Pectoris Seattle Angina Questionnaire

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B-1 cells modulate the murine macrophage response to Leishmania major infection

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World Journal of Biological Chemistry 2017年第2期8卷 151-162页

作者： Angelica F Arcanjo Marise P nunes Elias B Silva-Junior Monique Leandro Juliana Dutra Barbosa da Rocha alexandre Morrot Debora Decote-Ricardo Celio Geraldo Freire-de-Lima Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio de Janeiro Instituto Oswaldo Cruz FIOCRUZ Instituto de Microbiologia Paulo de Góes Universidade Federal do Rio de Janeiro Instituto de Veterinária Universidade Federal Rural do Rio de Janeiro

AIM To investigate the modulatory effect of B-1 cells on murine peritoneal macrophages infected with Leishmania major(L. major) in *** Peritoneal macrophages obtained from BALB/c andBALB/c XID mice were infected with L. major and cultured in the presence or absence of B-1 cells obtained from wild-type BALB/c mice. Intracellular amastigotes were counted, and interleukin-10(IL-10) production was quantified in the cellular supernatants using an enzymelinked immunosorbent assay. The levels of the lipid mediator prostaglandin E2(PGE2) were determined using a PGE2 enzyme immunoassay kit(Cayman Chemical, Ann Arbor, MI), and the number of lipid bodies was quantified in the cytoplasm of infected macrophages in the presence and absence of B-1 cells. Culturing the cells with selective PGE2-neutralizing drugs inhibited PGE2 production and confirmed the role of this lipid mediator in IL-10 production. In contrast, we demonstrated that B-1 cells derived from IL-10 KO mice did not favor the intracellular growth of L. *** We report that B-1 cells promote the growth of L. major amastigotes inside peritoneal murine macrophages. We demonstrated that the modulatory effect was independent of physical contact between the cells, suggesting that soluble factor(s) were released into the cultures. We demonstrated in our co-culture system that B-1 cells trigger IL-10 production by L. major-infected macrophages. Furthermore, the increased secretion of IL-10 was attributed to the presence of the lipid mediator PGE2 in supernatants of L. major-infected macrophages. The presence of B-1 cells also favors the production of lipid bodies by infected macrophages. In contrast, we failed to obtain the same effect on parasite replication inside L. major-infected macrophages when the B-1 cells were isolated from IL-10 knockout mice. CONCLUSION Our results show that elevated levels of PGE2 and IL-10 produced by B-1 cells increase L. major growth, as indicated by the number of parasites

关键词： Leishmania major Macrophages B-1 cells Interleukin-10 Prostaglandin E2 Infection

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New Sulfonamides Derived from Carvacrol: Compounds with High Antibacterial Activity against Resistant Staphylococcus aureus Strains

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Journal of Biosciences and Medicines 2016年第7期4卷 105-114页

作者： Aldo Sena de Oliveira Luana Canzian Llanes Inês Maria Costa Brighente Ricardo José nunes Rosendo Augusto Yunes Nilton Máximo Junior Ana Milda Karsten Baumgart Anays Noumeh Aust alexandre Bella Cruz Department of Chemistry Federal University of Santa Catarina Florianópolis Brazil Graduate Program in Pharmaceutical Sciences University of Valley of ltajaí Itajaí Brazil

Nine new sulfonamides derived from carvacrol were prepared through a reaction between 4-hydroxy-2-isopropyl-5-methyl benzene sulfonyl chloride with various amines in excellent yields (76% - 92%). The sulfonamides were characterized using spectrometric and spectroscopic methods. Among these compounds, three derivatives showed excellent results in antibacterial activity against resistant S. aureus strains, with MIC values ranging from 3.9 to 62.50 ppm. The sulfonamide derivative of 4-methylaniline (SULF-1) had the best performance for all tested strains of bacteria (MIC = 3.9 to 15.62 ppm). Furthermore, the sulfonamide derivative of 4-fluoro aniline (SULF-3), which also presented promising results, was found to have a synergistic effect when combined with tetracycline and partial synergistic effect when combined with ampicillin, exhibiting an FIC index between 0.50 and 0.75. The sulfonamide derivative of 4-methylaniline had a synergistic effect in combination with erythromycin exhibiting an FIC index of 0.37. Carvacrol in association with the antibiotics tested did not have a synergistic effect.

关键词： Sulfonamides Carvacrol Antibacterial Staphylococcus aureus Synergism

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