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Chemical Composition,Antioxidant and Antimicrobial Activity of the Oil and Plant Extract Myrocarpus frondosus Allemao

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American Journal of Plant Sciences 2017年第7期8卷 1560-1571页

作者： Ivandra Ignês de Santi Darci Alberto Gatto Miriam Ribeiro Galvao Machado Patrícia soares Bilhalva dos santos Rogerio Ant?nio Freitag Natural Products Research Laboratory Graduate Program in Biochemistry and Bioprospecting Federal University of Pelotas Pelotas Brasil Postgraduate Program Materials Science and Engineering Federal University of Pelotas Pelotas Brasil Center for Chemical Sciences Pharmaceutical and Food Federal University of Pelotas Pelotas Brasil Chemical and Environmental Engineering Department University of the Basque Country (UPV/EHU) San Sebastian Spain

The objective of this study was to evaluate the chemical profile of extracts (aqueous and ethanol) and the essential oil of Myrocarpus frondosus Allem?o, the sensitivity of strains of Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Listéria monocytogenes, front the extracts and essential oil, by means of the microdilution method in broth, the antioxidant activity by the ABTS method, and the content of phenolic compounds present in the extracts and oil. For the preparation of extracts from plant leaves used with ethanol and water, then separated, the chemical identification of compounds was performed by high-performance liquid chromatography (CLAE-DAD) and gas chromatography coupled to mass spectrum (CG/MS). With the chemical analysis of the extracts obtained the presence of the major compound rutin, and oil major compound was found germacrene B. In the microdilution method in broth, oil and extracts showed inhibition against all the bacteria tested in the concentrations 1 mg/ml to 0.25 mg/ml, except for Staphylococcus aureus at a concentration of 0.25 mg/ml of the essential oil and trans-caryophyllene. The results of Minimum Bactericidal Concentration showed that the essential oil had bactericidal activity at a concentration of 1mg/ml for all bacteria tested and trans-caryophyllene at the same concentration only for Listeria monocytogenes. In relation to the essential oil, antioxidant activity showed higher radical reduction capacity of 40.92% and the content of phenolic compounds ethanol extract showed more 12.72%. The in vitro results support the conclusion that the essential oil is very promising both in antimicrobial action as antioxidant activity and the leaf extracts on antioxidant activity.

关键词： Myrocarpus frondosus Allemao Antimicrobial Antioxidant

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Rapid Differentiation of Closely Related Citrus Genotypes by Fluorescence Spectroscopy

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Advances in Bioscience and Biotechnology 2014年第11期5卷 903-914页

作者： Dayse Drielly Souza Santana-Vieira Debora Marcondes Bastos Pereira Milori Paulino Ribeiro Villas Boas Marina Franca e Silva Magno Guimaraes santos Fernanda Amato Gaiotto Walter dos santos soares Filho Abelmon da Silva Gesteira Embrapa Cassava and Fruits Cruz das AlmasBrazil Department of Biological Sciences State University of Santa CruzIlheusBrazil Embrapa Instrumentation Sao CarlosBrazil

The differentiation of closely related Citrus genotypes is a meticulous, laborious, and time-consuming task that involves the assessment of complex traits such as growth, tolerance to stress, photosynthetic efficiency, yield, and many others. Such a task is generally accomplished either by analyzing specific features of adult plants or by applying molecular markers to young trees. On one hand, only after plants start yielding can distinct genotypes be differentiated by comparing their fruit sizes, shapes, taste, and the number of seeds. On the other hand, molecular markers are expensive, and demand expertise and time for the analysis of a larger number of plants. For these reasons, the development of techniques that could assist in an early, quick and accurate differen-tiation of closely related Citrus varieties is of utmost importance. In this context, laser-induced fluorescence spectroscopy (LIFS) is a promising technique, since it is rapid, highly sensitive, and inexpensive. Previous studies showed that LIFS can differentiate a variety of sweet orange. However, this new study aimed to determine LIFS accuracy in the differentiation and grouping of very closely varieties of four Sunki mandarin selections: Comum, Florida, Tropical, and Maravilha. Furthermore we compared the results with ISSR and SSR molecular markers for the same varieties. LIFS technique distinguished the four selections with accuracy greater than 70%. Only with molecular markers was possible distinguishing clearly Tropical from Maravilha, but not Comum from Florida selections. In this way the results suggest that LIFS may be a sound tool for helping the identification of closely Citrus varieties.

关键词： Differentiation of Citrus Genotypes Sunki LIFS Molecular Markers PLSR

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Propranolol hydrochloride degradation using La@TiO_(2) functionalized with CMCD

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Journal of Rare Earths 2022年第4期40卷 579-585,I0003页

作者： Gustavo Lopes Colpani Vitor Follmann santos Rubieli Carla Frezza Zeferino Micheli Zanetti Josiane Maria Muneron de Mello Luciano Luiz Silva Natan Padoin Regina de Fatima Peralta Muniz Moreira Márcio Antônio Fiori Cíntia soaresc Post-Graduate Program in Technology and Innovation Management Community University of ChapecóRegionChapecó89809-900Brazil Department of Chemical Engineering Community University of ChapecóRegionChapecó89809-900Brazil Laboratory of Materials and Scientific Computing(LabMAC) Department of Chemical and Food EngineeringFederal University of Santa CatarinaFlorianópolis88040-900Brazil

Propranolol hydrochloride can be considered a persistent and bioaccumulative pharmaceutical in the *** drug and its by-products are potentially toxic and have adverse effects,since these compounds have been associated with endocrine-disrupting effects,reproductive deficiencies,embryo abnormalities and pericardial ***_(2)–La 0.05%–carboxymethyl-β-cyclodextrin(CMCD)nanoparticles were successfully prepared by a simple two-step method,which consists of sonification and *** characterization analyses reveal that lanthanum is dispersed on the semiconductor surface,probably forming Ti–O–La bonds,which can induce oxygen vacancies and surface defects that effectively restrain the recombination of photogenerated electron/holes *** efficiency of TiO_(2)–La 0.05%–CMCD samples in degradation of propranolol under UV-light irradiation is higher than that of pristine TiO_(2) within 20 min reaction,probably due to complex formation between theβ-blocker and the oligosaccharide,which allows us to propose a photocatalytic mechanism based on the formation of intermediates and competition of these compounds to the radicals and CMCD cavities.

关键词： TiO_(2) Lanthanum CMCD Propranolol Photocatalysis Functionalization

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Effect of Osmotic Stress on the Initial Development of Bean Seedlings

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American Journal of Plant Sciences 2014年第13期5卷 1973-1982页

作者： Pablo Diego Silva Cabral Leonardo N.S.dos santos Henrique Duarte Vieira Tais Cristina Bastos soares Cintia Aparecida Bremenkamp Weverton Pereira Rodrigues University of North Rio de Janeiro State Darcy Ribeiro Rio de JaneiroBrazil Faculty of Agricultural Engineering University of CampinasSao PauloBrazil Agricultural Sciences Center Federal University of Espirito SantoVictoriaBrazil Instituto Capixaba de Pesquisa e Extensao Rural—INCAPER VictoriaBrazil

One of the methods to determine the tolerance of plants to water stress is the observation of germination of seeds under stress induced in laboratory conditions. Thus, the aim was to evaluate the response of four common bean cultivars to water deficit simulated under laboratory conditions. Two common landrace genotypes acquired from farmers (FORT 07 and FORT 08) and two commercial cultivars, IAPAR 81 and Serrano were evaluated. The different levels of water deficit were induced with mannitol solution. The experimental design was completely randomized and a factorial scheme 5 × 4 (osmotic potential x genotypes), with four replications. The characteristics: primary root protrusion, seed vigor, normal and abnormal seedling, seedling and root length, fresh and dry weight of seedling and root were evaluated. By reducing the osmotic potential seedling vigor bean was more affected than the primary root protrusion. Regardless of cultivar, there was a significant and progressive reduction in the normal seedlings percentage from potential -0.3 MPa. The IAPAR 81 and FORT 07 cultivars showed higher tolerance to water deficit compared to other cultivars.

关键词： Phaseolus vulgaris L. Mannitol Water Stress

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Conception of a Web Operation System for Processing Petroleum Related Drilling Data: A Focus on Pre-Salt Real-Time Automation and Optimization

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Journal of Software Engineering and Applications 2019年第4期12卷 61-71页

作者： Yuri soares Pinheiro Lucas Campos Vieira Andreas Nascimento Francisco de Assis Souza dos santos Mauro Hugo Mathias Gerhard Thonhauser Asad Elmgerbi Julian Hunt Department of Engineering and Technology Petroleum Engineering Programs Universidade Federal do Espirito Santo (UFES/DETEC) Sao Mateus Brazil Department of Computational and Electronics Universidade Federal do Espirito Santo (UFES/DCEL) Sao Mateus Brazil Department of Petroleum Engineering Chair of Drilling and Completion Engineering Montanuniversit&#228 t Leoben (MUL/DPE/CDC) Leoben Austria International Institute of Applied System Analysis (IIASA/ASA-ENE) Laxenburg Austria Mechanic Department Faculty of Engineering Universidade Estadual Paulista (UNESP/FEG-DME/PRH48-ANP) Guaratingueta Brazil

Petroleum and Natural Gas still represent a considerable share in terms of energy consumption in the current global matrix, so that its exploration/exploitation is present in the market and driving activities in locations of specific complexities, as the ones along unconventional hydrocarbon resources from the Brazilian pre-salt. The daily cost of well drilling under harsh conditions can exceed US $1 million a day, turning any type of downtime or necessary maintenance during the activities to be very costly, moment in which processes optimization starts to be a key factor in costs reduction. Thus, new technologies and methods in terms of automating and optimizing the processes may be of great advantages, having its impact in total related project costs. In this context, the goal of this research is to allow a computation tool supporting achieving a more efficient drilling process, by means of drilling mechanics parameters choosiness aiming rate of penetration (ROP) maximization and mechanic specific energy (MSE) minimization. Conceptually, driven by the pre-operational drilling test curve trends, the proposed system allows it to be performed with less human influences and being updateable automatically, allowing more precision and time reduction by selecting optimum parameters. A Web Operating System (Web OS) was designed and implemented, running in online servers, granting accessibility to it with any device that has a browser and internet connection. It allows processing the drilling parameters supplied and feed into it, issuing outcomes with optimum values in a faster and precise way, allowing reducing operating time.

关键词： Trend Curves Web OS Optimization Pre-Salt Petroleum

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Fertilization with Nitrogen, Phosphorus and Potassium in Upland Rice Cultivars in the Southern Region of Rondônia, Brazil

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American Journal of Plant Sciences 2015年第14期6卷 2263-2271页

作者： Edimar Rodrigues soares Robertt Fernandes Laércio da Silva Londero Leandro Galon Fabiana Ferreira Pires Marcelo de Andrade Barbosa Diego Lopes dos santos Samara Cristina Sampaio Correa Everton Augusto Sampaio Correa Remy Carvalho dos santos Department of Soil Science S&atilde o Paulo State University (UNESP) Jaboticabal Brazil Department of Agronomy Federal Institute of Education Science and Technology of Rond&ocirc nia (IFRO) Colorado do Oeste Brazil Department of Agronomy College Amazon (FAMA) Vilhena Brazil Department Herbology Federal University of the Border South (UFFS) Erechim Brazil Federal University Mato Grosso (UFMT) Cuiaba Brazil

The upland rice productivity in the state of Rond?nia is still low, in view of the potential of culture. The use of cultivars adapted to different regions and more responsive to fertilizer employed is an essential practice which can change that. The aim of this study was to evaluate the agronomic characteristics and productivity of two upland rice cultivars with nitrogen, phosphorus and potassium (N-P-K) doses in two municipalities in the southern state of Rondonia region. The experimental design was a randomized block design with four replications in a factorial 2 × 2 × 5, with the first factor composed of two cultivars (hybrid Ecco and conventional farming AN Cambará), the second factor, the environment of the two municipalities, Cerejeiras and Vilhena and the third factor of five doses of N-P-K (0-0-0, 30-40-30, 60-60-60, 90-90-90 and 120-100-120 kg·ha-1). The characteristics evaluated were: tillering, number of integers and sterile grains per panicle, weight of 1000 grains, grain yield (kg·ha-1) and whole grain yield. There was no triple interaction between the three factors for any of the traits. The hybrid Ecco has higher tillering ability than AN Cambara and presents fewer sterile grains per panicle, heavier 1000 grains and hence greater productivity. For both cultivars, the highest yields are obtained with a dose of 120-100-120 kg·ha-1 N-P-K. For the Ecco, the productivity is achieved with this dose and the dose of 90-90-90 kg·ha-1 is statistically similar. There is no difference in productivity between the municipalities when the dose of N-P-K is less than 60-60-60 kg·ha-1. The highest yield of whole grains in function of N-P-K fertilization is obtained in Cerejeiras.

关键词： Oryza sativa L. Productivity Hybridization

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Hygienic and Sanitary Conditions of Eggs Commercialized in Town Fairs and Markets

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Food and Nutrition Sciences 2018年第7期9卷 855-867页

作者： Wanessa Karine da Silva Lima Ludmilla Santana soares Barros Ricardo Mendes da Silva Tamiles Barreto de Deus Danuza das Virgens Lima Adriana dos santos Silva Center for Agrarian Environmental and Biological Sciences Federal University of Rec&ocirc ncavo da Bahia Cruz das Almas Brazil

High nutritional content coupled with low cost and ease of purchase justifies the frequent use of chicken eggs. Even with technological innovations in the production, storage, and distribution of eggs, food poisoning continues to occur. Therefore, in the present study, we 1) evaluated the microbiological profile and the hygienic and sanitary quality of eggs commercialized in town fairs and markets and 2) verified whether there is any correlation between microbiological results and commercialization conditions. Thirty-six samples were collected from six municipalities in the region of Rec?ncavo from Bahia, Brazil. The hygienic and sanitary conditions were analyzed by quantifying the total coliforms, E. coli, mesophilic and psychrotrophic microorganisms, molds, and yeasts, and by the presence of Salmonella sp. The observational analysis showed that 91% and 68% of the samples in town fairs and markets, respectively, did not comply with the requirements of the current Brazilian legislation. There was no significant difference in the microbiological profile among samples from the fairs and markets. Several samples presented relevant significant values, indicating inadequate hygienic conditions and fecal contamination. Salmonella sp. was found in one sample. There was co-relationship between microorganism count and the variables “external dirt”, “external stains”, and “closeness to other products”, with high concentrations of total coliforms and E. coli in the non-conforming samples. Although no limits exist for mold and yeast counts, the presence of fungi makes the product unfit for consumption. Thus, the commercialization of eggs would benefit from more attention and supervision by the relevant authorities.

关键词： Contamination Egg Salmonella Microorganism Indicator Hygiene Condition

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Patogenic and Indicator Microorganisms in Chicken Cuts Sold in the Recôncavo-Bahia-Brazil

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Food and Nutrition Sciences 2017年第11期8卷 1028-1038页

作者： Wanessa Karine Da Silva Lima Ludmilla Santana soares Barros Ricardo Mendes Da Silva Tamiles Barreto De Deus Adriana Dos santos Silva Danuza Das Virgens Lima Centro de Ciências Agrárias Ambientais e Biológicas Universidade Federal do Rec&ocirc ncavo da Bahia Cruz das Almas Bahia

Hygiene-sanitary conditions, the presence of Salmonella spp. and indicator microorganisms of chicken cuts sold on the market in the Rec?ncavo da Bahia area, are analyzed and their co-relationship with conditions of commercialization is verified. Sixty samples were collected between May and August 2016 at informal selling sites (fairs, butcher shops, abattoirs) and in supermarkets of ten municipalities of the Rec?ncavo da Bahia region. Hygiene and sanitary conditions were assessed through a check list. Total coliforms, Escherichia coli, mesophylls, psychrotrophic organisms, molds and yeast, and Salmonella spp. were counted. Results revealed statistical difference for total coliforms and Escherichia coli only, featuring greater concentration rates in fairs, butcher shops and abattoirs. Further, 31.7% of samples indicated 31.7% Salmonella spp. and 16.6% of samples failed to comply with sanitary legislation on Escherichia coli. Observational analysis demonstrated that 61.83% and 38.1% of the samples respectively on informal sites and in supermarkets did not comply with legislation and indicated a co-relationship of some variables with the growth of total coliforms, Escherichia coli and mesophylls. Pathogenic and indicator microorganisms suggest flaws in hygiene and sanitary conditions in the commercialization of chicken cuts and health risks to consumers.

关键词： Microbiological Contamination Hygiene and Sanitary Conditions Salmonella Chickens

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Agronomic Performance of Cultivars of Upland Rice in the Southern of the Region of Rondonia,Brazil

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Agricultural Sciences 2014年第6期5卷 513-518页

作者： Edimar Rodrigues soares Robertt Fernandes Laercio da Silva Londero Diego Lopes dos santos Samara Cristina Sampaio Correa Everton Augusto Sampaio Correa Remy Carvalho dos santos Ariel Pereira Gomes Leandro Galon Fabiana Ferreira Pires Rosilene da Silva Goncalves Department of Soil Science Sao Paulo State University(UNESP)JaboticabalBrazil Department of Agronomy Federal Institute of Education Science and Technology of Rondonia(IFRO)Colorado do OesteBrazil Department of Agronomy College Amazon(FAMA)VilhenaBrazil Agronomist Federal University Mato Grosso(UFMT)CuiabáBrazil Department Herbology Federal University of the Southern Border South(UFFS)ErechimBrazil

The upland rice is grown in most Brazilian states. However, in many of them the yield obtained is low. The choice of cultivar more suited to a particular environment can contribute to increased productivity of this crop. This research aimed to evaluate the performance of a hybrid cultivar of upland rice (Ecco) and five conventional cultivars (BRS Monarca, BRS Primavera, AN Cambará, BRS Sertaneja and BRS MG Curinga) in two environments (Vilhena and Cerejeiras). The tests were conducted in the agricultural year 2010/2011. We evaluated the following characteristics: tillering, panicle number per unit area, number of filled grains per panicle sterile, mass of grains per panicle, 1000 grain weight, grain yield (kg·ha-1) and whole grain yield. There was no cultivar x environment interaction for all traits. Greater tillering, higher panicle number per area, higher productivity and yield of whole grains in the municipality of Cerejeiras were observed, while the highest number of sterile grains per panicle was obtained in Vilhena. The hybrid Ecco highlighted in relation to all other cultivars is in relation to tillering, panicle number per area and grain yield. The municipality of Cerejeiras is the most suitable for the cultivation of upland rice.

关键词： Oryza sativa L. Hybrid Upland Rice Genotype x Environment

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Application of Neuro-Fuzzy Estimation for Rotor Flux in Speed Vector Control of Bearingless Induction Motor

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Journal of Energy and Power Engineering 2015年第2期9卷 192-198页

作者： Jose soares Batista Lopes Luciano P. dos santos Jose A. de Paiva Valci F. Victor Andre L. Maitelli Andres O. Salazar Institute of Education Science and Technology of Rio Grande do Norte RN 59200-000 Brazil Institute of Education Science and Technology of Tocantis TO 77.015-200 Brazil Department of Computer Engineering and Automation Federal University of Rio Grande do Norte Natal 59072-970 RN Brazil

The main requirement of a vector controller is knowing the magnitude and position of the rotating flow in the rotor. This feature permits to use either flow sensors or flow estimators. The solution chosen was the estimation of rotor flux with the hybrid neuro-fuzzy system. The motor characteristics are： 3.75 kW （5 HP）, two pole-pair, operate at 60 Hz and air-gap length 0.2 mm. The ANFIS （adaptive neuro-fuzzy inference system） was used to tune the membership functions in fuzzy system. The hybrid estimator aims at compensating possible parametric variations of the machine caused by agents, such as temperature or nucleus saturation. The simulated results have shown good performance.

关键词： Hybrid systems rotor flux estimation speed control ANFIS.

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