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World journal of Transplantation 2024年第2期14卷 126-134页

作者： Gabriel Lazzarotto-da-Silva Leandro A Scaffaro mauricio Farenzena Lucas Prediger Rafaela K Silva Flávia Heinz Feier Tomaz j m Grezzana-Filho Pablo D Rodrigues alexandre de Araujo mario Reis Alvares-da-Silva Roberta C marchiori Cleber Rosito Pinto Kruel marcio Fernandes Chedid Department of Liver Transplant and Hepatobiliary Surgery Unit Hospital de Clínicas de Porto AlegrePorto Alegre 90035-007Brazil Department of Interventional Radiology Unit Hospital de Clínicas de Porto AlegrePorto Alegre 90035-007Brazil Department of Gastroenterology and Hepatology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-007Brazil

BACKGROUND Hepatocellular carcinoma(HCC)is an aggressive malignant neoplasm that requires liver transplantation(LT).Despite patients with HCC being prioritized by most organ allocation systems worldwide,they still have to wait for long *** therapies(LRTs)are employed as bridging therapies in patients with HCC awaiting *** largely used in the past,transarterial embolization(TAE)has been replaced by transarterial chemoembolization(TACE).However,the superiority of TACE over TAE has not been consistently shown in the *** To compare the outcomes of TACE and TAE in patients with HCC awaiting *** All consecutive patients with HCC awaiting LT between 2011 and 2020 at a single center were *** patients underwent LRT with either TACE or *** patients also underwent percutaneous ethanol injection(PEI),concom-itantly or in different treatment *** choice of LRT for each HCC nodule was determined by a multidisciplinary *** primary outcome was waitlist dropout due to tumor progression,and the secondary outcome was the occurrence of adverse *** the subset of patients who underwent LT,complete pathological response and post-transplant recurrence-free survival were also *** Twelve(18.5%)patients in the TACE group(only TACE and TACE+PEI;n=65)and 3(7.9%)patients in the TAE group(only TAE and TAE+PEI;n=38)dropped out of the waitlist due to tumor progression(P log-rank test=0.29).Adverse events occurred in 8(12.3%)and 2(5.3%)patients in the TACE and TAE groups,respectively(P=0.316).Forty-eight(73.8%)of the 65 patients in the TACE group and 29(76.3%)of the 38 patients in the TAE group underwent LT(P=0.818).Among these patients,complete pathological response was detected in 7(14.6%)and 9(31%)patients in the TACE and TAE groups,respectively(P=0.145).Post-LT,HCC recurred in 9(18.8%)and 4(13.8%)patients in the TACE and TAE groups,respectively(P=0.756).Posttransplant recurrence-free survival was simila

关键词： Hepatocellular carcinoma Transarterial embolization Transarterial chemoembolization Liver transplantation Locoregional therapy Bridging

Environmental determinants of social wasp diversity and assemblage structure in an Amazonian archipelagic landscape

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Zoological Research(Diversity and Conservation) 2024年第2期1卷 121-129页

作者： jose Victor Alves Ferreira alexandre Somavilla maíra Benchimol Ana Filipa Palmeirim Carlos A.Peres Danielle Storck-Tonon Laboratório de Ecologia Aplicada àConservação Universidade Estadual de Santa CruzIlhéus 45662-900Brazil Instituto Nacional de Pesquisas da Amazônia Coordenação de BiodiversidadeManaus 69067-001Brazil CIBIO Centro de Investigação em Biodiversidade e Recursos GenéticosInBIO Laboratório AssociadoCampus de VairãoUniversidade do PortoVairão 4485-661Portugal BIOPOLIS Program in Genomics Biodiversity and Land PlanningCIBIOCampus de VairãoVairão 4485-661Portugal School of Environmental Sciences University of East AngliaNorwich NR47TJUK Instituto juruá Manaus69057-060Brazil Programa de Pós-Graduação em Ambiente e Sistemas de Produção Agrícola(PPGASP) Universidade do Estado de Mato GrossoTangaráda Serra 78300-970Brazil

Hydropower development has become an important driver of habitat loss and fragmentation across lowland tropical *** ample evidence on the detrimental effects of insular habitat fragmentation on biodiversity,invertebrate taxa,that may be critical to ecosystem functioning,have been *** assessed the assemblage-level responses of social wasps to forest insularization induced by the Balbina Hydroelectric Dam in Central Brazilian *** malaise trapping,we captured wasps on 27 forest islands and three continuous *** constructed Generalized Linear models and employed a model selection approach to examine the impact of local variables(fire severity(FIRE)and basal area of pioneer tree species(PIONEER))and landscape-scale variables(amount of habitat(COVER))on patterns of species richness,composition,and body size of wasps.A total of 374 individuals(29 species)were collected across all sampling *** was the main predictor of species richness,while PIONEER was the only variable that explained variation in community composition,with a negative effect on body *** results add evidence to the pervasive impacts of large hydroelectric dams on tropical forest biodiversity,and suggest that social wasps,among other invertebrates,can be used as bioindicators in infrastructure development projects.

关键词： Habitat amount hypothesis Habitat fragmentation Habitat loss Hydroelectric dams Invertebrates

In the lack of extreme pioneers:trait relationships and ecological strategies of 66 subtropical tree species

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journal of Plant Ecology 2015年第4期8卷 359-367页

作者： Cristiane Forgiarini alexandre F.Souza Solon j.Longhi juliano m.Oliveira Programa de Pós-Graduação em Biologia Diversidade e Manejo da Vida SilvestreUniversidade do Vale do Rio dos SinosAv.UNISINOS 950C.P.275São Leopoldo 93022-000RSBrazil Departamento de Ecologia CBUniversidade Federal do Rio Grande do NorteCampus UniversitárioLagoa NovaNatal 59072-970RNBrazil PPG-Engenharia Florestal Dep.de Ciências FlorestaisUniversidade Federal de Santa MariaAv.Roraimas/nBairro CamobiSanta Maria 97105-900RSBrazil Present address:Departamento de Botânica Instituto BiociênciasUniversidade Federal do Rio Grande do SulAv.Bento Gonçalves9500Prédio 43433Campus AgronomiaPorto Alegre 91501-970RSBrazil.

Aims Despite the growing interest in the topic of functional ecology,there are still forest regions that have not been examined,as most work has been done in the *** issues include the strength of a growth-mortality trade-off in trees(originally identified for seedlings)and the nature of the association between plant traits and vital rates,if *** objectives were to examine whether(i)ecological strategies in south american mixed forests are organ-ized along the fast competitor×slow stress-tolerator and height gradients as the main strategy axes depicted in the overall trait and vital rate correlation structure,and(ii)a tentative path model we proposed can explain the patterns of covariation among traits and vital *** We studied a different habitat(subtropical mixed conifer-hardwood forests)and region(brazilian atlantic Forest)from the majority of related studies in forests,carried out in the Neotropical *** on total height,stem slenderness,crown depth,wood density,specific leaf area,leaf and seed length,seed dispersal mode,annual mortality,diameter relative growth rate and relative growth rate under favorable conditions were measured in southern brazil for 66 tree *** were subjected to principal components analysis and path *** data on saplings and treelets were analyzed through *** Findingsstudied traits were reduced to four principal *** components analysis produced axes that fit the resource acquisition versus resource-conservation and the height-mortality trade-offs,although the former was split into two distinct *** size and seed dispersal mode appeared independently of these axes.a path model showed that leaf length and specific leaf area caused direct changes in trunk slenderness and,indirectly through growth,affected *** trade-offs between growth and survivorship and between wood density and stem slenderness trade-offs were not *** may

关键词： Araucaria angustifolia atlantic forest ecological strategies life-history trade-offs mixed forests

A combinatorial indexing strategy for low-cost epigenomic profiling of plant single cells

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Plant Communications 2022年第4期3卷 72-82页

作者： Xiaoyu Tu alexandre P.marand Robert j.Schmitz Silin Zhong joint Center for Single Cell Biology School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Shanghai Collaborative Innovation Center of Agri-Seeds School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Department of Genetics University of GeorgiaAthensGA 30602USA State Key Laboratory of Agrobiotechnology School of Life SciencesThe Chinese University of Hong KongHong KongChina

Understanding how cis-regulatory elements facilitate gene expression is a key question in *** advances in single-cell genomics have led to the discovery of cell-specific chromatin landscapes that underlie transcription programs in animal ***,the high equipment and reagent costs of commercial systems limit their applications for many *** this study,we developed a combinatorial index and dual PCR barcode strategy to profile the Arabidopsis thaliana root single-cell epigenome without any specialized *** generated chromatin accessibility profiles for 13576 root nuclei with an average of 12784 unique Tn5 integrations per *** of the single-cell assay for transposaseaccessible chromatin sequencing and RNA sequencing data sets enabled the identification of 24 cell clusters with unique transcription,chromatin,and cis-regulatory *** with single-cell data generated using the commercial microfluidic platform from 10X Genomics revealed that this low-cost combinatorial index method is capable of unbiased identification of cell-type-specific chromatin *** anticipate that,by removing cost,instrumentation,and other technical obstacles,this method will be a valuable tool for routine investigation of single-cell epigenomes and provide new insights into plant growth and development and plant interactions with the environment.

关键词： combinatorial indexing single-cell ATAC-seq epigenome

Nanoscale image of the drug/metal mono-layer interaction:Tapping A Fm-IR investigations

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Nano Research 2020年第4期13卷 1020-1028页

作者： Natalia Piergies alexandre Dazzi Ariane Deniset-Besseau jeremie mathurin magdalena Ocwieja Czeslawa Paluszkiewicz Wojciech m.Kwiatek Institute of Nuclear Physics Polish Academy of SciencesPL-31342 KrakowPoland Laboratoire de Chimie Physique(LCP) CNRS UMR 8000Univ.of Paris-SudUniversite Paris-Saclay91405 OrsayFrance jerzy Haber Institute of Catalysis and Surface Chemistry Polish Academy of Sciences Niezapominajek 8PL-30239 KrakowPoland

The application of metal nanoparticles as an efficient drug delivery system is one of the directions of cancer therapy ***,this strategy requires precise information about how the drug interacts with the applied *** this study,atomic force microscopy combined with infrared spectroscopy(AFm-IR)was used for the first time to investigate the erlotinib adsorption structure on two different types of 15 nm metal nanoparticle mono-layers,namely,silver nanoparticle(AgNP)and gold nanoparticle(AuNP)*** the metal nanoparticles are loosely bound samples,only the tapping AFm-IR mode is suitable for the collection of IR maps and spectra for such a *** obtained results indicated the relevance of the AFm-IR technique for characterizing drug interactions with a metal mono-layer *** investigated drug interacts with the AgNPs mainly through phenyl rings and methoxy moieties,while quinazoline,amino,and ethoxy moieties appear to be farther from the *** the AuNPs,the interaction occurs through both the phenyl ring and the quinazoline ***,the aliphatic groups of erlotinib directly participate in this *** novelty of the present work is also related to the use of the tapping AFm-IR mode to study metal NP mono-layers with a drug adsorbed on *** collected IR maps for the most enhanced erlotinib bands show specific areas with very high signal *** connection between these areas and the"hot spots"typical for the surface plasmon resonance phenomenon of metals is considered.

关键词： metal nanoparticle mono-layer drug's adsorption infrared nanospectroscopy atomic force microscopy erlotinib

Theplant cytosolic m^(6)A RNA methylome stabilizes photosynthesis in the cold

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Plant Communications 2023年第6期4卷 70-84页

作者： alexandre magno Vicente Nikolay manavski Paul Torben Rohn Lisa-marie Schmid Antoni Garcia-molina Dario Leister Charlotte Seydel Leo Bellin Torsten möhlmann Gregor Ammann Stefanie Kaiser jörg meurer Plant molecular Biology Faculty of BiologyLudwig-Maximilians-University MunichGroßhaderner Street 2-482152 Planegg-MartinsriedGermany Plant Development Faculty of BiologyLudwig-Maximilians-University MunichGroßhaderner Street 2-482152 Planegg-MartinsriedGermany Plant Physiology Faculty of BiologyUniversity of KaiserslauternErwin-Schröodinger-Street767663 KaiserslauternGermany Pharmaceutical Chemistry Goethe University FrankfurtMax-von-Laue-Str.960438 Frankfurt am MainGermany Present address:Loewe Biochemica GmbH Möuhlweg 2a82054 SauerlachGermany Present address:Center for Research in Agricultural Genomics 08193

The sessile lifestyle of plants requires an immediate response to environmental stressors that affect photosynthesis,growth,and crop ***,we showed that three abiotic perturbations—heat,cold,and high light—triggered considerable changes in the expression signatures of 42 epitranscriptomic factors(writers,erasers,and readers)with putative chloroplast-associated functions that formed clusters of commonly expressed genes in *** expression changes under all conditions were reversible upon deacclimation,identifying epitranscriptomic players as modulators in acclimation *** dysfunctions,particularly those induced by the oxidative stress-inducing norflurazon in a largely GENOmE UNCOUPLED-independent manner,triggered retrograde signals to remodel chloroplastassociated epitranscriptomic expression patterns.N6-methyladenosine(m^(6)A)is known as the most prevalent RNA modification and impacts numerous developmental and physiological functions in living *** cold treatment,expression of components of the primary nuclear m^(6)A methyltransferase complex was upregulated,accompanied by a significant increase in cellular m^(6)A mRNA *** the cold,the presence of FIP37,a core component of the writer complex,played an important role in positive regulation of thylakoid structure,photosynthetic functions,and accumulation of photosystemⅠ,the Cytb6f complex,cyclic electron transport proteins,and Curvature Thylakoid1 but not that of photosystemⅡcomponents and the chloroplast ATP *** of FIP37 affected abundance,polysomal loading,and translation of cytosolic transcripts related to photosynthesis in the cold,suggesting m^(6)Adependent translational regulation of chloroplast *** summary,we identified multifaceted roles of the cellular m^(6)A RNA methylome in coping with cold;these were predominantly associated with chloroplasts and served to stabilize photosynthesis.

关键词： m^(6)A RNA methylation cold acclimation stress response Arabidopsis thaliana photosynthesis chloroplast

The Deep Thioredoxome in Chlamydomonas reinhardtii： New Insights into Redox Regulation

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molecular Plant 2017年第8期10卷 1107-1125页

作者： maria Esther Perez-Perez Adeline mauries alexandre maes Nicolas j. Tourasse marion Hamon Stephane D. Lemaire Christophe H. marchand Institut de Biologie Physico-Chimique UMR8226 CNRS Sorbonne Universit6s UPMC Univ Paris 06 13 rue Pierre et Marie Curie 75005 Paris France Institut de Biologie Physico-Chimique Plateforme de Proteomique FRC550 CNRS 13 rue Pierre et Marie Curie 75005 Paris France Instituto de Bioquimica Vegetal y Fotosintesis Consejo Superior de Investigaciones Cientificas (CSIC)-Universidad de Sevilla Seville Spain ARNA Laboratory INSERM U1212 CNRS UMR5320 Universite Bordeaux 2 Bordeaux France

Thiol-based redox post-translational modifications have emerged as important mechanisms of signaling and regulation in all organisms, and thioredoxin plays a key role by controlling the thiol-disulfide status of target proteins. Recent redox proteomic studies revealed hundreds of proteins regulated by glutathio- nylation and nitrosylation in the unicellular green alga Chlamydomonas reinhardtii, while much less is known about the thioredoxin interactome in this organism. By combining qualitative and quantitative proteomic analyses, we have comprehensively investigated the Chlamydomonas thioredoxome and 1188 targets have been identified. They participate in a wide range of metabolic pathways and cellular pro- cesses. This study broadens not only the redox regulation to new enzymes involved in well-known thiore- doxin-regulated metabolic pathways but also sheds light on cellular processes for which data supporting redox regulation are scarce （aromatic amino acid biosynthesis, nuclear transport, etc）. moreover, we char- acterized 1052 thioredoxin-dependent regulatory sites and showed that these data constitute a valuable resource for future functional studies in Chlamydomonas. By comparing this thioredoxome with proteomic data for glutathionylation and nitrosylation at the protein and cysteine levels, this work confirms the existence of a complex redox regulation network in Chlamydomonas and provides evidence of a tremendous selectivity of redox post-translational modifications for specific cysteine residues.

关键词： Chlamydomonas reinhardtii disulfide bond isotope-coded affinity tag redox proteomics redox regulation thioredoxin targets

Livestock induces strong spatial heterogeneity of soil CO2, N2O and CH4 emissions within a semi-arid sylvo-pastoral landscape in West Africa

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journal of Arid Land 2017年第2期9卷 210-221页

作者： mohamed H ASSOUmA Dominique SERCA Frederic GUERIN Vincent BLANFORT Philippe LECOmTE Ibra TOURE alexandre ICKOWICZ Raphael j mANLAY martial BERNOU jonathan VAYSSIERES CIRAD-UmR Selmet Campus International Baillarguet Montpellier 34398 France DP PPZS ISRA-LNERV BP 2057 Dakar Hann Senegal Laboratoire d'Aerologie-OmP Universite Paul Sabatier 14 Avenue Edouard Belin Toulouse 31400 France Geosciences Environnement Toulouse UmR 5563 & UR 234 IRD Universite Paul Sabatier Avenue Edouard Belin 14 Toulouse 31400 France CILSS 03 BP 7049 Ouagadougou Burkina Faso AgroParisTech-UmR Eco&Sols 2 place Viala Montpellier SupAgro Montpellier 34060 France IRD-UmR Eco&Sols 2 place Viala Montpellier SupAgro Montpellier 34060 France

Greenhouse gas （GHG） emissions from the surface soils and surface water receiving animal excreta may be important components of the GHG balance of terrestrial ecosystems, but the associated processes are poorly documented in tropical environments, especially in tropical arid and semi-arid areas. A typical sylvo-pastoral landscape in the semi-arid zone of Senegal, West Africa, was investigated in this study. The study area （706 km2 of managed pastoral land） was a circular zone with a radius of 15 km centered on a borehole used to water livestock. The landscape supports a stocking rate ranging from 0.11 to 0.39 tropical livestock units per hectare depending on the seasonal movements of the livestock. Six landscape units were investigated （land in the vicinity of the borehole, natural ponds, natural rangelands, forest plantations, settlements, and enclosed plots）. Carbon dioxide （CO2）, nitrous oxide （N2O） and methane （CH4） fluxes were measured with static chambers set up at 13 sites covering the six landscape units, and the 13 sites are assumed to be representative of the spatial heterogeneity of the emissions. A total of 216 fluxes were measured during the one-year study period （may 2014 to April 2015）. At the landscape level, soils and surface water emitted an average 19.8 t C-CO2 eq/（hm2.a）（CO2： 82%, N2O： 15%, and CH4： 3%）, but detailed results revealed notable spatial heterogeneity of GHG emissions. CO2 fluxes ranged from 1148.2 （±91.6） mg/（m2.d） in rangelands to 97,980.2 （±4,861.7） mg/（m2.d） in surface water in the vicinity of the borehole. N2O fluxes ranged from 0.6 （±0.1） mg/（m2.d） in forest plantations to 22.6 （±10.8） mg/（m2.d） in the vicinity of the borehole. CH4 fluxes ranged from -3.2 （±0.3） mg/（m2.d） in forest plantations to 8788.5 （±2295.9） mg/（m2.d） from surface water in the vicinity of the borehole. This study identified GHG emission ＂hot spots＂ in the landscape. Emissions from the surface soils were significantly higher in the landscape units most freq

关键词： greenhouse gases soil surface water livestock landscape Senegal

Pain inhibition through transplantation of fetal neuronal progenitors into the injured spinal cord in rats

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Neural Regeneration Research 2019年第11期14卷 2011-2019页

作者： Chary m. Batista Eric D. mariano Camila S. Dale alexandre F. Cristante Luiz R. Britto jose P. Otoch manoel j. Teixeira matthias morgalla Guilherme Lepski Department of Neurology School of MedicineUniversity of Sao PauloSao PauloBrazil Department of Anatomy Institute of Biomedical ScienceUniversity of Sao PauloSao PauloBrazil Department of Orthopedic and Traumatology School of MedicineUniversity of Sao PauloSao PauloBrazil Department of Physiology and Biophysics Institute of Biomedical ScienceUniversity of Sao PauloSao PauloBrazil Department of Surgery School of MedicineUniversity of Sao PauloSao PauloBrazil Department of Neurosurgery Eberhard-Karls UniversityTuebingenGermany Department of Psychiatry School of MedicineUniversity of Sao PauloSao PauloBrazil

Neuropathic pain after spinal cord injury(SCI) is a complex condition that responds poorly to usual treatments. Cell transplantation represents a promising therapy;nevertheless, the ideal cell type in terms of neurogenic potential and effectiveness against pain remains largely controversial. Here, we evaluated the ability of fetal neural stem cells(fNSC) to relieve chronic pain and, secondarily, their effects on motor recovery. Adult Wistar rats with traumatic SCI were treated, 10 days after injury, with intra-spinal injections of culture medium(sham) or fNSCs extracted from telencephalic vesicles(TV group) or the ventral medulla(Vm group) of E/14 embryos. Sensory(von Frey filaments and hot plate) and motor(the Basso, Beattie,Bresnahan locomotor rating scale and inclined plane test) assessments were performed during 8 weeks. Thereafter, spinal cords were processed for immunofluorescence and transplanted cells were quantified by stereology. The results showed improvement of thermal hyperalgesia in the TV and Vm groups at 4 and 5 weeks after transplantation, respectively. moreover, mechanical allodynia improved in both the TV and Vm groups at 8 weeks. No significant motor recovery was observed in the TV or Vm groups compared with sham. Stereological analyses showed that ~70% of TV and Vm cells differentiated into NeuN+ neurons,with a high proportion of enkephalinergic and GABAergic cells in the TV group and enkephalinergic and serotoninergic cells in the Vm group. Our study suggests that neuronal precursors from TV and Vm, once implanted into the injured spinal cord, maturate into different neuronal subtypes, mainly GABAergic, serotoninergic, and enkephalinergic, and all subtypes alleviate pain, despite no significant motor recovery. The study was approved by the Animal Ethics Committee of the medical School of the University of S?o Paulo(protocol number 033/14) on march 4, 2016.

关键词： spinal cord injuries chronic pain neural stem cells cell transplantation neuronal differentiation GABAergic neuron serotoninergic neuron enkephalinergic neuron