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Detection and Characterization of the Cyanobacteria and Cyanophages of Barnegat Bay, New Jersey

Journal of Environmental Protection 2019年第11期10卷 1472-1483页

作者： Roksana M. Rahman alexandra greco Alisha Nanda Christian Rios-Ruiz Yan Wang Paul Yoon Dena J. Restaino John J. Gaynor Paul A. X. Bologna Tinchun Chu Department of Biological Sciences Seton Hall University NJ USA Department of Biology and Chemistry County College of Morris Randolph NJ USA Department of Biology Montclair State University NJ USA

Cyanobacterial harmful algal blooms (CHAB), caused by eutrophication, are known to threaten both wildlife and human health. Due to urbanization and land use changes, an increase of CHAB’s at a more frequent rate within Barnegat Bay has been observed. In order to detect possible CHAB causing cyanobacteria, water samples were collected from 12 different locations within Barnegat Bay. Each sample was filtered through a 30- and 0.4-μm polycarbonate filter sequentially. Flow cytometry was carried out for the filtrate collected between 0.4- and 30-μm. Chelex DNA extraction, polymerase chain reaction (PCR), and gel electrophoresis were then performed for all sites using four primer sets (27F/785R, PSF/UR, CYA359F/CYA781R and MSF/MSR) designed to detect cyanobacteria. Flow cytometric results indicated the water samples contained a wide range of cyanobacteria, including M. aeruginosa, Cylindrospermum spp., Anabaena spp., and Synechococcus sp. IU 625 ranges from 3.16 to 8.17 × 107 cells·L-1. PCR-based assays suggest that general cyanobacteria as well as phytospecific species were present for all sites, but no toxin-producing Microcystis aeruginosa was detected. Plaque assays demonstrated the presence of cyanophages for S. IU 625, Anabaena spp., and M. aeruginosa at all sites, up to 105 PFU·ml-1.

关键词： Cyanobacterial Harmful Algal Blooms Eutrophication Flow Cytometry Polymerase Chain Reaction Plaque Assay

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