检索结果-南通市图书馆

Treating amyotrophic lateral sclerosis with allogeneic Schwann cell-derived exosomal vesicles: a case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第4期20卷 1207-1216页

作者： Pascal j.Goldschmidt-Clermont Aisha Khan George jimsheleishvili Patricia Graham Adriana Brooks Risset Silvera alexander j.P.Goldschmidt Damien D.Pearse W.Dalton Dietrich Allan D.Levi james D.Guest Miller School of Medicine University of MiamiMiamiFLUSA Stem Cell Institute Miller School of MedicineUniversity of MiamiMiamiFLUSA The Miami Project to Cure Paralysis Miller School of MedicineUniversity of MiamiMiamiFLUSA Alzady International LLC MiamiFLUSA Neurological Surgery Miller School of MedicineUniversity of MiamiMiamiFLUSA

Schwann cells are essential for the maintenance and function of motor neurons,axonal networks,and the neuromuscular *** amyotrophic lateral sclerosis,where motor neuron function is progressively lost,Schwann cell function may also be ***,important signaling and potential trophic activities of Schwann cell-derived exosomal vesicles have been *** case report describes the treatment of a patient with advanced amyotrophic lateral sclerosis using serial intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles,marking,to our knowledge,the first instance of such *** 81-year-old male patient presented with a 1.5-year history of rapidly progressive amyotrophic lateral *** initial diagnosis,the patient underwent a combination of generic riluzole,sodium phenylbutyrate for the treatment of amyotrophic lateral sclerosis,and *** patient volunteered to participate in an FDA-approved single-patient expanded access treatment and received weekly intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles to potentially restore impaired Schwann cell and motor neuron *** confirmed that cultured Schwann cells obtained from the amyotrophic lateral sclerosis patient via sural nerve biopsy appeared impaired(senescent)and that exposure of the patient’s Schwann cells to allogeneic Schwann cell-derived exosomal vesicles,cultured expanded from a cadaver donor improved their growth capacity in *** a period of observation lasting 10 weeks,during which amyotrophic lateral sclerosis Functional Rating Scale-Revised and pulmonary function were regularly monitored,the patient received weekly consecutive infusions of 1.54×1012(×2),and then consecutive infusions of 7.5×1012(×6)allogeneic Schwann cell-derived exosomal vesicles diluted in 40 mL of Dulbecco’s phosphate-buffered *** of the infusions were associated with adverse events such as infusion reactions(allergic or otherwise)or

关键词： allogeneic amyotrophic lateral sclerosis exosomes infusion neuromuscular junction Schwann cell

Immunological battlefield in gastric cancer and role of immunotherapies

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World journal of Gastroenterology 2016年第28期22卷 6373-6384页

作者： Minyu Wang Rita A Busuttil Sharon pattison Paul j Neeson Alex Boussioutas Cancer Genetics and Genomics Laboratory Peter MacCallum Cancer Centre East Melbourne 3002 Victoria Australia Sir Peter MacCallum Department of Oncology The University of Melbourne Parkville 3050 Victoria Australia Department of Medicine The University of Melbourne Royal Melbourne Hospital Parkville 3050 Victoria Australia Department of Medicine Dunedin School of Medicine University of Otago Dunedin 9016 New Zealand Southern Blood and Cancer Service Southern District Health Board Dunedin 9054 New Zealand Cancer Immunology Research Peter MacCallum Cancer Centre East Melbourne 3002 Victoria Australia Pathology Department University of Melbourne Parkville 3050 Victoria Australia

Like the wars predating the First World War where human foot soldiers were deemed tools in the battlefield against an enemy, so too are the host immune cells of a patient battling a malignant gastric cancer. Indeed, the tumour microenvironment resembles a battlefield, where the patient's immune cells are the defence against invading tumour cells. However, the relationship between different immune components of the host response to cancer is more complex than an "us against them" model. Components of the immune system inadvertently work against the interests of the host and become pro-tumourigenic while other components soldier on against the common enemy – the tumour cell.

关键词： Immune Gastric cancer Immune therapy Immunology Tumour microenvironment

Guanylyl cyclase C signaling axis and colon cancer prevention

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World journal of Gastroenterology 2016年第36期22卷 8070-8077页

作者： Amanda M pattison Dante j Merlino Erik S Blomain Scott A Waldman Department of Pharmacology and Experimental Therapeutics Thomas Jefferson University

Colorectal cancer(CRC) is a major cause of cancerrelated mortality and morbidity worldwide. While improved treatments have enhanced overall patient outcome, disease burden encompassing quality of life, cost of care, and patient survival has seen little benefit. Consequently, additional advances in CRC treatments remain important, with an emphasis on preventative measures. Guanylyl cyclase C(GUCY2C), a transmembrane receptor expressed on intestinal epithelial cells, plays an important role in orchestrating intestinal homeostatic mechanisms. These effects are mediated by the endogenous hormones guanylin(GUCA2A) and uroguanylin(GUCA2B), which bind and activate GUCY2 C to regulate proliferation, metabolism and barrier function in intestine. Recent studies have demonstrated a link between GUCY2 C silencing and intestinal dysfunction, including tumorigenesis. Indeed, GUCY2 C silencing by the near universal loss of its paracrine hormone ligands increases colon cancer susceptibility in animals and humans. GUCY2C's role as a tumor suppressor has opened the door to a new paradigm for CRC prevention by hormone replacement therapy using synthetic hormone analogs, such as the FDA-approved oral GUCY2 C ligand linaclotide(Linzess^(TM)). Here we review the known contributions of the GUCY2 C signaling axis to CRC, and relate them to a novel clinical strategy targeting tumor chemoprevention.

关键词： Colorectal cancer Guanylin Uroguanylin Chemoprevention Heat-stable enterotoxins Cyclic guanosine monophosphate Guanylyl cyclase C

Factors associated with 5-year survival of combined hepatocellular and cholangiocarcinoma

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World journal of Hepatology 2020年第11期12卷 1020-1030页

作者： Tomoki Sempokuya Eric A Wien Robert j.pattison jihyun Ma Linda L Wong Division of Gastroenterology and Hepatology Department of Internal MedicineUniversity of Nebraska Medical CenterOmahaNE 68198United States Department of Medicine John A.Burns School of MedicineUniversity of Hawaii at ManoaHonoluluHI 96813United States Department of Biostatistics College of Public HealthUniversity of Nebraska Medical CenterOmahaNE 68198United States Department of Surgery John A.Burns School of MedicineUniversity of Hawaii at ManoaHonoluluHI 96817United States

BACKGROUND Combined hepatocellular and cholangiocarcinoma(HCC/CC)is a rare primary hepatic malignancy which carries a poor prognosis due to its aggressive *** centers have enough cases to draw definitive conclusions and there is limited understanding of *** the rarity of HCC/CC,an analysis of large national cancer database was needed to obtain larger number of HCC/CC *** To identify associated factors for 5-year survival of HCC/*** We conducted a retrospective study of The Surveillance,Epidemiology,and End Results(SEER)database obtained from SEER*Stat 8.3.6 *** defined histology code 8180 for the International Classification of Disease for Oncology,3rd edition was used to identify HCC/CC cases from 2004 to *** collected demographics,American joint Committee on Cancer(AjCC)stage,treatment,tumor size,and survival *** data were converted to categorical *** Shapiro-Wilk normality test was used to assess normal ***-Whitney U test was used to compare continuous variables without normal distribution,and t-test was used to compare continuous variables with a normal *** Kaplan-Meier survival curve analyzed 5-year *** and multivariate logistic regression model was used to analyze factors associated with 5-year *** Cox proportional hazard regression was done on 5-year *** defined P<0.05 was statistically *** We identified 497 patients with the following characteristics:Mean age 62.4 years(SD:11.3),149(30.0%)were female,racial distribution was:276(55.5%)white,53(10.7%)black,84(16.9%)Asian and Pacific Islander(API),77(15.5%)Hispanic,and 7(1.4%)others or *** I/II disease occurred in 41.5%and tumor size<50 mm was seen in 35.6%of ***-four(4.8%)received locoregional therapy(LRT),119(23.9%)underwent resection,and 50(10.1%)underwent liver *** overall median survival was 6 mo[Interquartile range(I

关键词： Combined hepatocellular and cholangiocarcinoma Surveillance,Epidemiology,and End Results database Survival Race Hepatobiliary cancer Transplant

Current clinical approach to achalasia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World journal of Gastroenterology 2009年第32期15卷 3969-3975页

作者： alexander j Eckardt Volker F Eckardt Central Interdisciplinary Endoscopy Unit Department of Gastroenterology and HepatologyCharité University Hospitals BerlinCampus VirchowD-13353 BerlinGermany Department of Gastroenterology Deutsche Klinik für DiagnostikD-65191 WiesbadenGermany

Idiopathic achalasia is a rare primary motility disorder of the esophagus. The classical features are incomplete relaxation of a frequently hypertensive lower esophageal sphincter (LES) and a lack of peristalsis in the tubular esophagus. These motor abnormalities lead to dysphagia, stasis, regurgitation, weight loss, or secondary respiratory complications. Although major strides have been made in understanding the pathogenesis of this rare disorder, including a probable autoimmune mediated destruction of inhibitory neurons in response to an unknown insult in genetically susceptible individuals, a definite trigger has not been identified. The diagnosis of achalasia is suggested by clinical features and conf irmed by further diagnostic tests, such as esophagogastroduodenoscopy (EGD), manometry or barium swallow. These studies are not only used to exclude pseudoachalasia, but also might help to categorize the disease by severity or clinical subtype. Recent advances in diagnostic methods, including high resolution manometry (HRM), might allow prediction of treatment responses. The primary treatments for achieving long-term symptom relief are surgery and endoscopic methods. Although limited high-quality data exist, it appears that laparoscopic Heller myotomy with partial fundoplication is superior to endoscopic methods in achieving long-term relief of symptoms in the majority of patients. However, the current clinical approach to achalasia will depend not only on patients' characteristics and clinical subtypes of the disease, but also on local expertise and patient preferences.

关键词： Achalasia Esophageal motility disorder Dysphagia Esophagus Lower esophageal sphincter Pneumatic dilation Botulinum toxin Heller myotomy

Measuring user preferences and behaviour in a topographic immersive virtual environment(TopoIVE)of 2D and 3D urban topographic data

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International journal of Digital Earth 2021年第12期14卷 1835-1867页

作者： Łukasz Halik alexander j.Kent Department of Cartography and Geomatics Adam Mickiewicz UniversityPoznańPoland School of Psychology and Life Sciences Canterbury Christ Church UniversityCanterburyUK

This paper investigates user preferences and behaviour associated with 2D and 3D modes of urban representation within a novel Topographic Immersive Virtual Environment(TopoIVE)created from official 1:10,000 *** participants were divided into two groups:the first were given a navigational task within a simulated city and the second were given the freedom to explore it.A Head-Mounted Display(HMD)Virtual Reality(VR)app allowed participants to switch between 2D and 3D representations of buildings with a remote controller and their use of these modes during the experiment was *** performed mental rotation tests before entering the TopoIVE and were interviewed afterwards about their experiences using the *** results indicate that participants preferred the 3D mode of representation overall,although preference for the 2D mode was slightly higher amongst those undertaking the navigational task,and reveal that different wayfinding solutions were adopted by participants according to their ***,the findings suggest that users exploit different aspects of 2D and 3D modes of visualization in their wayfinding strategy,regardless of their *** potential to combine the functionality of 2D and 3D modes therefore offers substantial opportunities for the development of immersive virtual reality products derived from topographic datasets.

关键词： Virtual reality(VR) immersive virtual environment(IVE) 2D versus 3D wayfinding topographic data

Cloning and functional characterization of two cDNAs encoding NADPH-dependent 3-ketoacyl-CoA reductased from developing cotton fibers

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2005年第6期15卷 465-473页

作者： Francois MA PUjOL,alexander j KASTANIOTIS,j Kalervo HILTUNEN Biocenter Oulu and Department of Biochemistry University of Oulu P.O. Box 3000 FIN-90014 University of Oulu Finland Biocenter Oulu and Department of Biochemistry University of Oulu P.O. Box 3000 FIN-90014 University of Oulu Finland Biocenter Oulu and Department of Biochemistry University of Oulu P.O. Box 3000 FIN-90014 University of Oulu Finland

Genes encoding enzymes involved in biosynthesis of very long chain fatty acids were significantly up-regulatedduring early cotton fiber development. Two cDNAs, GhKCR1 and GhKCR2 encoding putative cotton 3-ketoacyl-CoAreductases that catalyze the second step in fatty acid elongation, were isolated from developing cotton fibers. GhKCR1and 2 contain open reading frames of 963 bp and 924 bp encoding proteins of 320 and 307 amino acid residues,respectively. Quantatitive RT-PCR analysis showed that both these genes were highly preferentially expressed duringthe cotton fiber elongation period with much lower levels recovered from roots, stems and leaves. GhKCR1 and 2showed 30%-32% identity to Saccharomyces cerevisiae Ybr159p at the deduced amino acid level. These cotton cDNAswere cloned and expressed in yeast haploid ybr159w? mutant that was deficient in 3-ketoacyl-CoA reductase ***-type growth rate was restored in ybr159w? cells that expressed either GhKCR1 or 2. Further analysis showed thatGhKCR1 and 2 were co-sedimented within the membranous pellet fraction after high-speed centrifugation, similar to theyeast endoplasmic reticulum marker ScKar2p. Both GhKCR(s) showed NADPH-dependent 3-ketoacyl-CoA reductaseactivity in an in vitro assay system using palmitoyl-CoA and malonyl-CoA as substrates. Our results suggest thatGhKCR1 and 2 are functional orthologues of ScYbr159p.

关键词： very-long-chain fatty acids endoplasmic reticulum fatty acid elongation system 3-ketoacyl-CoA reductase Gossypium hirsutum short-chain alcohol dehydrogenase/reductase protein family.

Forces and energetics of intermittent swimming

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Mechanica Sinica 2017年第4期33卷 725-732页

作者： Daniel Floryan Tyler Van Buren alexander j.Smits Department of Mechanical and Aerospace Engineering Princeton University Princeton NJ 08544 USA

Experiments are reported on intermittent swimming motions. Water tunnel experiments on a nominally two-dimensional pitching foil show that the mean thrust and power scale linearly with the duty cycle, from a value of 0.2 all the way up to continuous motions, indicating that individual bursts of activity in intermittent motions are independent of each other. This conclusion is corroborated by particle image velocimetry（PIV） flow visualizations, which show that the main vortical structures in the wake do not change with duty cycle. The experimental data also demonstrate that intermittent motions are generally energetically advantageous over continuous motions. When metabolic energy losses are taken into account, this conclusion is maintained for metabolic power fractions less than 1.

关键词： intermittent swimming maintained conclusion cycle thrust favorable producing aquatic circles

Interaction of Radiation and Turbulent Natural Convection:A Pseudo-Direct Numerical Study

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Advances in Applied Mathematics and Mechanics 2022年第6期14卷 1567-1586页

作者： alexander Nee Ali j.Chamkha Research and Educational Center of I.N.Butakov National Research Tomsk Polytechnic UniversityTomskRussia Faculty of Engineering Kuwait College of Science and TechnologyDoha DistrictKuwait

This paper presents a hybrid lattice Boltzmann solver for turbulent buoyancy-driven flow coupled with surface thermal *** two-relaxation time scheme for the Boltzmann equation combined with the implicit finite difference scheme for the energy equation is implemented to compute the heat transfer and fluid flow *** accuracy and robustness of the hybrid approach proposed in this study are assessed in terms of the numerical and experimental data of other *** performing the simulation,the Rayleigh number is ranged from 108 to 1010 whereas the surface emissivity is changed from zero to *** computations,it is found that the overall temperature of the cavity is increased as a result of enhancing the surface *** plumes are formed both at the isothermal and the thermally-insulated walls with the Ra109 and#*** the conditions under study,the overall heat transfer rate is raised by around 5%when taking into account the surface thermal radiation.

关键词： Pseudo-direct numerical simulation surface radiation hybrid lattice Boltzmann scheme turbulent natural convection.