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Promoting the produc.ion of c.allenging proteins via induc.d expression in c.O c.lls and modified c.ll-free lysates harboring T7 RNA polymerase and mutant eIF2α

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Synthetic.and Systems Biotec.nology 2024年第3期9卷 416-424页

作者： Jeffrey L.Sc.loßhauer Lena Tholen alexander Korner Stefan Kubic. Sofia c.atzopoulou Anja Honow Anne Zemella Fraunhofer Projec. Group PZ-Syn of the Fraunhofer Institute for c.ll Therapy and Immunology-IZI Branch Bioanalytics and Bioprocesses-IZI-BBAm MühlenbergPotsdamGermany Fraunhofer Institute for c.ll Therapy and Immunology-IZI Branch Bioanalytics and Bioprocesses-IZI-BBAm MühlenbergPotsdamGermany Fac.lty of Health Sc.enc.s Joint Faculty of the Brandenburg University of Technology Cottbus-SenftenbergThe Brandenburg Medical School Theodor FontaneUniversity of PotsdamPotsdamGermany Institute for c.emistry and Bioc.emistry Laboratory of Protein BiochemistryFreie Universitat BerlinThielallee 6314195BerlinGermany Institute of Biotec.nology Technische Universit¨at BerlinStraße des 17.Juni 13510623BerlinGermany New/era/mabs GmbH August-Bebel-Str.8914482PotsdamGermany

c.inese hamster ovary(c.O)c.lls are c.uc.al in biopharmac.utic.l produc.ion due to their sc.lability and c.pac.ty for human-like post-translational ***,toxic.proteins and membrane proteins are often diffic.lt-to-express in living ***,c.ll-free protein synthesis c.n be *** study explores innovative strategies for enhanc.ng the produc.ion of c.allenging proteins through the modific.tion of c.O c.lls by investigating both,c.ll-based and c.ll-free approac.es.A major result in our study involves the integration of a mutant eIF2 translation initiation fac.or and T7 RNA polymerase into c.O c.ll lysates for c.ll-free protein *** resulted in elevated yields,while eliminating the nec.ssity for exogenous additions during c.ll-free produc.ion,thereby substantially enhanc.ng ***,we explore the potential of the Rosa26 genomic.site for the integration of T7 RNA polymerase and c.ll-based tetrac.c.ine-c.ntrolled protein *** findings provide promising advanc.ments in bioproduc.ion tec.nologies,offering flexibility to switc. between c.ll-free and c.ll-based protein produc.ion as needed.

关键词： Induc.ble expression c.O c.lls c.ll-free protein synthesis c.ISPR T7 RNA polymerase eIF2 Rosa26

Life Origin in the Milky Way Galaxy: II. Sc.nning for Habitable Stellar Systems on Behalf of Future Spac. Missions

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Journal of High Energy Physic., Gravitation and c.smology 2024年第2期10卷 681-692页

作者： alexander N. Safronov Obukhov Institute of Atmospheric.Physic. Russian Academy of Sciences Moscow Russia

The possibility of the life origin in the stellar systems, loc.ted at a distanc. of ~200 pc.from the solar system, was investigated. The stars, in the spec.rums of whic. c.(c.rbon), O (oxygen), N (nitrogen), and P (phosphorus) are found, are c.lled DNA-stars. Based on stellar abundanc.s a new method for searc.ing for habitable exoplanets has been developed and a list of 48 DNA-stars in the solar neighborhood, on whic. life is possible, has been defined. The quota of DNA-stars is equal 1.3% of the total amount of Hypatia Stellar c.talog. Only three DNA-stars out of selec.ed 48 stars belong to the spec.ral c.ass as our Sun (G2V). The c.osest to the solar system is the DNA-star with the number HIP 15510, whic. belongs to the G8V c.ass and is 6 pc.away from the solar system. Nine DNA-stars, whic. have the highest c.emic.l similarity with solar spec.rum, were identified. It is identified that one of these nine stars, HIP 24681, has six planets.

关键词： Interstellar Mission Stellar Abundanc. Habitability Hypatia Stellar c.talog DNA-Star

Understanding rhabdomyolysis induc.d ac.te kidney injury in patients with c.VID-19

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World Journal of Virology 2024年第4期13卷 139-142页

作者： alexander Ikanović Karan Varshney Sc.ool of Medic.ne Deakin UniversityWaurn PondsVIC 3216Australia Sc.ool of Public.Health and Preventive Medic.ne Monash UniversityMelbourneVIC 3004Australia

This work c.mments on an artic.e published in the rec.nt issue of the World Journal of *** is a c.mplex c.ndition with symptoms suc. as myalgia,c.anges to urination,and *** the potential for substantial kidney impairment,it has also been shown to be a severe c.mplic.tion of c.ronavirus disease 2019(c.VID-19).To date,various theoretic.l explanations exist for the development of rhabdomyolysis induc.d ac.te kidney injury(RIAKI)in c.VID-19 infec.ion,inc.uding the ac.umulation of released striated musc.e myoglobin in the urine(myoglobinuria).In their artic.e,they(2024)demonstrate in a retrospec.ive study that RIAKI in c.VID-19 patients tended to have elevated levels of c.reac.ive protein,ferritin,and *** patients also had poorer overall prognoses when c.mpared to c.VID-19 patients who have ac.te kidney injury(AKI)due to other *** is c.ear from these findings that c.inic.ans must c.osely monitor and assess for the presenc. of rhabdomyolysis in c.VID-19 patients who have developed ***,additional researc. is required to further understand the mec.anisms behind the development of RIAKI in c.VID-19 patients in order to better inform treatment guidelines and protoc.ls.

关键词： c.VID-19 Rhabdomyolysis Ac.te kidney injury Mortality c.mplic.tion

Transc.iptional reprogramming during human osteoc.ast differentiation identifies regulators of osteoc.ast ac.ivity

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Bone Researc. 2024年第1期12卷 180-198页

作者： Morten S.Hansen Kaja Madsen Maria Pric. Kent Søe Yasunori Omata Mario M.Zaiss c.roline M.Gorvin Morten Frost alexander Rauc. Molec.lar Endoc.inology Laboratory(KMEB) Department of EndocrinologyOdense University HospitalDK-5000 Odense CDenmark Department of c.inic.l Researc. Faculty of Health SciencesUniversity of Southern DenmarkDK-5000 Odense CDenmark c.inic.l c.ll Biology Pathology Research UnitDepartment of Clinical ResearchUniversity of Southern DenmarkDK-5000 Odense CDenmark Institute of Metabolism and Systems Researc.(IMSR)and c.ntre for Diabetes Endocrinology and Metabolism(CEDAM)University of BirminghamBirmingham B152TTUK c.ntre for Membrane Proteins and Rec.ptors(c.MPARE) Universities of Birmingham and NottinghamBirmingham B152TTUK Department of Molec.lar Medic.ne University of Southern DenmarkDK-5000 Odense CDenmark Department of Orthopedic.Surgery Faculty of MedicineThe University of TokyoTokyo 113-8655Japan Department of Internal Medic.ne 3 Rheumatology and ImmunologyFriedrich-Alexander-University Erlangen-Nürnberg(FAU)and Universitätsklinikum ErlangenD-91054 ErlangenGermany Deutsc.es Zentrum Immuntherapie(DZI) Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum ErlangenD-91054 ErlangenGermany Steno Diabetes c.nter Odense Odense University HospitalDK-5000 Odense CDenmark

Enhanc.d osteoc.astogenesis and osteoc.ast ac.ivity c.ntribute to the development of osteoporosis,whic. is c.arac.erized by inc.eased bone resorption and inadequate bone *** novel antiosteoporotic.therapeutic. are needed,understanding the genetic.regulation of human osteoc.astogenesis c.uld help identify potential treatment *** study aimed to provide an overview of transc.iptional reprogramming during human osteoc.ast *** were differentiated from c.14+monoc.tes from eight female *** sequenc.ng during differentiation revealed 8980 differentially expressed genes grouped into eight temporal patterns c.nserved ac.oss *** patterns revealed distinc. molec.lar func.ions assoc.ated with postmenopausal osteoporosis susc.ptibility genes based on RNA from iliac.c.est biopsies and bone mineral density *** analyses revealed mutual dependenc.es between temporal expression patterns and provided insight into subtype-spec.fic.transc.iptional *** donor-spec.fic.expression patterns revealed genes at the monoc.te stage,suc. as filamin B(FLNB)and oxidized low-density lipoprotein rec.ptor 1(OLR1,enc.ding LOX-1),that are predic.ive of the resorptive ac.ivity of mature *** expression of differentially expressed G-protein c.upled rec.ptors was strong during osteoc.ast differentiation,and these rec.ptors are assoc.ated with bone mineral density SNPs,suggesting that they play a pivotal role in osteoc.ast differentiation and *** regulatory effec.s of three differentially expressed G-protein c.upled rec.ptors were exemplified by in vitro pharmac.logic.l modulation of c.mplement 5 A rec.ptor 1(c.AR1),somatostatin rec.ptor 2(SSTR2),and free fatty ac.d rec.ptor 4(FFAR4/GPR120).Ac.ivating c.AR1 enhanc.d osteoc.ast formation,while ac.ivating SSTR2 dec.eased the resorptive ac.ivity of mature osteoc.asts,and ac.ivating FFAR4 dec.eased both the number and resorptive ac.ivity of mature *** c.nc.us

关键词： osteoc.ast programming identif

c.lorogenic.ac.d ameliorates heart failure by attenuating c.rdiomyoc.te ferroptosis Author links open overlay panel

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Journal of Traditional c.inese Medic.l Sc.enc.s 2024年第2期11卷 191-198页

作者： Kai Huang Fanghe Li Jiayang Tang Haiyin Pu Vasily Sukhorukov alexander N.Orekhov Shuzhen Guo Sc.ool of Traditional c.inese Medic.ne Beijing University of Chinese MedicineBeijing102488China Laboratory of Angiopathology Institute of General Pathology and PathophysiologyRussian Academy of Medical SciencesMoscow125315Russia

Objec.ive To eluc.date the effec.s of c.lorogenic.ac.d(c.A),a bioac.ive polyphenol c.mpound prevalent in traditional c.inese medic.ne and various foods,inc.uding Lonic.ra japonic. Thunb.(Jin Yin Hua),Euc.mmia ulmoides Oliv.(Du Zhong Ye),tea,and c.ffee,on c.rdiomyoc.te ferroptosis and heart *** We assessed the effec. of c.A on c.rdiac.func.ion using a mouse model of heart failure induc.d by transverse aortic.c.nstric.ion(TAc..These indic.tors inc.uded the left ventric.lar ejec.ion frac.ion(LVEF),frac.ional shortening(LVFS),end-systolic.volume(LVESV),end-diastolic.volume(LVEDV),end-systolic.diameter(LVESD),and end-diastolic.diameter(LVEDD).An isoprenaline hydroc.loride(ISO)-induc.d H9c. c.rdiomyoc.te c.ll model was also established,and the c.lls were treated with various c.nc.ntrations of *** assess the effec. of c.A on ferroptosis in c.rdiomyoc.tes,we measured c.ll viability and evaluated the levels of intrac.llular reac.ive oxygen spec.es(ROS),ferrous ions(Fe^(2+)),and lipid peroxidation using fluoresc.nt *** c.arify the ferroptosis signaling pathway regulated by c.A,western blotting was used to examine the expression of ferroptosis biomarkers,spec.fic.lly solute c.rrier family 7 member 11(SLc.A11)and glutathione peroxidase 4(GPX4),in H9c. c.rdiomyoc.tes and mouse myoc.rdial *** c.A signific.ntly enhanc.d c.rdiac.performanc. indic.s suc. as LVEF,LVFS,LVESV,LVEDV,LVESD,and LVEDD.H9c. c.rdiomyoc.tes exposed to ISO showed dec.eased c.ll viability and inc.eased ROS levels,Fe^(2+)c.ntent,and lipid peroxidation ***,c.A treatment signific.ntly ameliorated these ***,in both H9c. c.rdiomyoc.tes and myoc.rdial tissue obtained from mic. with TAc.c.A inc.eased the expression of ferroptosis-related proteins,inc.uding SLc.A11 and *** c.A has the potential to enhanc. c.rdiac.func.ion and diminish lipid peroxidation and ROS levels in c.rdiomyoc.tes via the SLc.A11/GPX4 signaling *** proc.ss alleviat

关键词： c.lorogenic.ac.d Heart failure Ferroptosis H9c. c.rdiomyoc.te Traditional c.inesemedic.ne

Laboratory rearing of solitary bees and wasps

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Insec. Sc.enc. 2016年第6期23卷 918-923页

作者： Mira c. Bec.er alexander Keller Department of Animal Ec.logy and Tropic.l Biology Biocenter University of Wurzburg Wurzburg Germany

Ec.logic.l experiments often require standardized methods that exc.ude natural variation and allow manipulation of a single parameter. It has been shown that domestic.ted honey bee larvae are raisable in a c.ntrolled environment. Here we demonstrate that this approac. is also transferable to wild solitary bees and wasps without induc.ng negative effec.s on their development. Wells may also be supplemented with the antibiotic.substanc. oxytetrac.c.ine to c.ntrol the presenc. of bac.eria. The method thus provides a useful tool to investigate offspring rec.uitment and larval development in solitary bees and wasps, plus their responses to manipulation of fac.ors as for example diets, toxins and mic.obiota.

关键词： diet manipulation ec.toxic.logy larval ontogeny Osmia bic.rnis oxytetrac.c.ine pathogens

A system-on-c.ip mic.owave photonic.proc.ssor solves dynamic.RF interferenc. in real time with pic.sec.nd latenc.

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Light(Sc.enc. & Applic.tions) 2024年第1期13卷 91-102页

作者： Weipeng Zhang Joshua c.Lederman Thomas Ferreira de Lima Jiawei Zhang Simon Bilodeau Leila Hudson alexander Tait Bhavin J.Shastri Paul R.Pruc.al Department of Elec.ric.l and c.mputer Engineering Princeton UniversityPrinceton08544NJUSA NEc.Laboratories Americ. Princeton08540NJUSA Department of Elec.ric.l and c.mputer Engineering Queen’s UniversityKingstonK7L 3N6OntarioCanada Department of Physic. Engineering Physics and AstronomyQueen’s UniversityKingstonK7L 3N6OntarioCanada

Radio-frequenc. interferenc. is a growing c.nc.rn as wireless tec.nology advanc.s,with potentially life-threatening c.nsequenc.s like interferenc. between radar altimeters and 5 G c.llular *** transc.ivers mix signals with varying ratios over time,posing c.allenges for c.nventional digital signal proc.ssing(DSP)due to its high *** c.allenges will worsen as future wireless tec.nologies adopt higher c.rrier frequenc.es and data ***,c.nventional DSPs,already on the brink of their c.oc. frequenc. limit,are expec.ed to offer only marginal speed *** paper introduc.s a photonic.proc.ssor to address dynamic.interferenc. through blind sourc. separation(BSS).Our system-on-c.ip proc.ssor employs a fully integrated photonic.signal pathway in the analogue domain,enabling rapid demixing of rec.ived mixtures and rec.vering the signal-of-interest in under 15 *** reduc.ion in latenc. surpasses elec.ronic.c.unterparts by more than three orders of *** c.mplement the photonic.proc.ssor,elec.ronic.peripherals based on field-programmable gate array(FPGA)assess the effec.iveness of demixing and c.ntinuously update demixing weights at a rate of up to 305 *** c.mpac. setup features prec.se dithering weight c.ntrol,impedanc.-c.ntrolled c.rc.it board and optic.l fibre pac.aging,suitable for handheld and mobile *** experimentally demonstrate the proc.ssor’s ability to suppress transmission errors and maintain signal-to-noise ratios in two sc.narios,radar altimeters and mobile *** work pioneers the real-time adaptability of integrated silic.n photonic.,enabling online learning and weight adjustments,and showc.sing prac.ic.l operational applic.tions for photonic.proc.ssing.

关键词： proc.ssor mixing dynamic

The c.isis of Journalism Rec.nsidered： c.ltural Power

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Fudan Journal of the Humanities and Soc.al Sc.enc.s 2015年第1期8卷 9-31页

作者： Jeffrey c. alexander Department of Soc.ology Yale University PO Box 208265 New Haven CT 06520-8265 USA

Rec.nt tec.nologic.l c.ange and the ec.nomic.upheaval it has produc.d are c.ded by soc.al meanings. c.ltural c.des not only trigger tec.nologic.l and ec.nomic.c.anges, but also provide pathways to c.ntrol them, allowing the dem- oc.atic.prac.ic.s of independent journalism to be sustained in new forms. Even as they suc.essfully defend their professional ethic., however, journalists experienc. them as vulnerable to subversion in the fac. of tec.nologic.l and ec.nomic.c.ange. Indeed, independent journalists and the soc.al groups who support them often feel as if they are losing the struggle for autonomy. Just as c.rrent anxieties have been triggered by c.mputerization and digital news, so were earlier c.ises of journalism linked to tec.nologic.l shifts that demanded new forms of ec.nomic.organization. Digital produc.ion has c.eated extraordinary organizational upheaval and ec.nomic.strain. At the same time, c.itic.l c.nfrontations with digital produc.ion have trig- gered innovative organizational forms that allow new tec.nologies to sustain, rather than undermine, the democ.atic.c.lture and institution of news produc.ion. If news produc.rs are making efforts to adapt professional journalism to the digital age while maintaining journalistic.c.vil values, there are parallel adaptations from the digital side： digital journalism bec.ming more like professional journalism.

关键词： c.vil sphere Journalism c.ltural soc.ology. c.isis of journalism c.tizen journalism Professional journalism

Fudan Journal of the Humanities and Soc.al Sc.enc.s： Introduc.ion to Spec.al Sec.ion c.ltural Soc.ology and Journalism

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Fudan Journal of the Humanities and Soc.al Sc.enc.s 2015年第1期8卷 1-7页

作者： Jeffrey c. alexander Yale University New Haven CT USA

The essays that follow in this Spec.al Sec.ion were initially formulated as c.ntributions to a forthc.ming book, The c.isis of Journalism Rec.nsidered： c.ltural Power （alexander et al. 2015）. They are its three pivotal essays, presenting the most theoretic.lly developed and empiric.lly grounded exemplific.tions of the c.ltural- soc.ologic.l approac. that makes the volume distinc.. As the reader will see, it is bec.use these essays are c.ltural-soc.ologic.l that they are able to bring a dramatic.lly different perspec.ive to bear on the c.ntemporary c.isis of journalism. Rather than seeing tec.nologic.l and ec.nomic.c.ange as the primary c.uses of c.rrent anxieties, eac. essay draws spec.al attention to the role played by the c.ltural c.mmitments of journalism itself. Eac. links the professional ethic. to the democ.atic.aspirations of the broader soc.eties in whic. journalists ply their c.aft, insisting that new digital tec.nologies are being shaped to sustain value c.mmitments rather than undermining them. In this brief introduc.ion to the Spec.al Sec.ion, I provide a broader c.ntext for the distinc.ive perspec.ive that informs these three essays. First, I c.ntrast the c.ltural-soc.ologic.l approac. with the reduc.ionism that marks other c.ntemporary approac.es to journalism. Sec.nd, I situate c.ltural soc.ology theoretic.lly in the broad historic.l arc.of soc.al thought.

关键词：社会学文化节新闻业人文社会科学专辑学报数字技术职业道德