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Increased connectivity of hiPSC-derived neural networks in multiphase granular hydrogel scaffolds

Bioactive materials 2022年第3期7卷 358-372页

作者： Chia-Chen Hsu Julian H.George Sharlayne Waller Cyril Besnard David A Nagel Eric J Hill michael D.Coleman alexander m.korsunsky Zhanfeng Cui Hua Ye Institute of Biomedical Engineering Department of Engineering ScienceUniversity of OxfordOX37DQUK mBLEm Department of Engineering ScienceUniversity of OxfordParks RoadOxfordOX13PJUK School of Biosciences College of Health and Life SciencesAston UniversityBirminghamB47ETUK Translational medicine Research Group Aston Medical SchoolCollege of Health and Life SciencesAston UniversityBirminghamB47ETUK

To reflect human development,it is critical to create a substrate that can support long-term cell survival,differentiation,and maturation.Hydrogels are promising materials for 3D cultures.However,a bulk structure consisting of dense polymer networks often leads to suboptimal microenvironments that impedes nutrient exchange and cell-to-cell interaction.Herein,granular hydrogel-based scaffolds were used to support 3D human induced pluripotent stem cell(hiPSC)-derived neural networks.A custom designed 3D printed toolset was developed to extrude hyaluronic acid hydrogel through a porous nylon fabric to generate hydrogel granules.Cells and hydrogel granules were combined using a weaker secondary gelation step,forming self-supporting cell laden scaffolds.At three and seven days,granular scaffolds supported higher cell viability compared to bulk hydrogels,whereas granular scaffolds supported more neurite bearing cells and longer neurite extensions(65.52±11.59μm)after seven days compared to bulk hydrogels(22.90±4.70μm).Long-term(three-month)cultures of clinically relevant hiPSC-derived neural cells in granular hydrogels supported well established neuronal and astrocytic colonies and a high level of neurite extension both inside and beyond the scaffold.This approach is significant as it provides a simple,rapid and efficient way to achieve a tissue-relevant granular structure within hydrogel cultures.

关键词： microgel Hydrogel Hyaluronan iPSC Neural tissue engineering 3D printing

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The olfactory system of Pieris brassicae caterpllars:from receptors to glomeruli

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Insect Science 2024年第2期31卷 469-488页

作者： Qi Wang Hans m.Smid marcel Dicke alexander Haverkamp Laboratory of Entomology Wageningen University and ResearchWageningenthe Netherlands

Tolfactory system of adult lepidopterans is among the best described neuronal circuits.However,comparatively little is known about the organization of the olfactory system in the larval stage of these insects.Here,we explore the expression of olfactory receptors and the organization of olfactory sensory neurons in caterpillars of Pieris brassicae,a significant pest species in Europe and a well-studied species for its chemical ecology.To describe the larval olfactory system in this species,we first analyzed the head transcriptome of third-instar larvae(L3)and identified 16 odorant receptors(ORs)including the OR coreceptor(Orco),13 ionotropic receptors(IRs),and 8 gustatory receptors(GRs).We then quantified the expression of these 16 ORs in different life stages,using qPCR,and found that the majority of ORs had significantly higher expression in the L4 stage than in the L3 and L5 stages,indicating that the larval olfactory system is not static throughout caterpillar development.Using an Orco-specific antibody,we identified all olfactory receptor neurons(ORNs)expressing the Orco protein in L3,L4,and L5 caterpillars and found a total of 34 Orco-positive ORNs,distributed among three sensilla on the antenna.The number of Orco-positive ORNs did not differ among the three larval instars.Finally,we used retrograde axon tracing of the antennal nerve and identified a mean of 15 glomeruli in the larval antennal center(LAC),suggesting that the caterpillar olfactory system follows a similar design as the adult olfactory system,although with a lower numerical redundancy.Taken together,our results provide a detailed analysis of the larval olfactory neurons in P brassicae,highlighting both the differences as well as the commonalities with the adult olfactory system.These findings contribute to a better understanding of the development of the olfactory system in insects and its life-stage-specific adaptations.

关键词： glomeruli larvae Lepidoptera odorant receptor olfactory receptor neurons sensilla

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High resolution ultrastructure imaging of fractures in human dental tissues

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Theoretical & Applied mechanics Letters 2014年第4期4卷 38-41页

作者： Tan Sui Siqi Ying Gabriel Landini alexander m.korsunsky Department of Engineering Science University of OxfordOxford OX1 3PJUnited Kingdom School of Dentistry University of BirminghamUnited Kingdom

Human dental hard tissues are dentine, cementum, and enamel. These are hydrated mineralised composite tissues with a hierarchical structure and versatile thermo-mechanical properties. The hierarchical structure of dentine and enamel was imaged by transmission electron microscopy （TEm） of samples prepared by focused ion beam （FIB） milling. High resolution TEm was carried out in the vicinity of a crack tip in dentine. An intricate ＂random weave＂ pattern of hydroxyapatile crystallites was observed and this provided a possible explanation for toughening of the mineralized dentine tissue at the nano-scale. The results reported here provide the basis for improved understanding of the rela- tionship between the multi-scale nature and the mechanical properties of hierarchically structured biomaterials, and will also be useful for the development of better prosthetic and dental restorative materials.

关键词： human dental tissue transmission electron microscopy focused ion beam,crack

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Transcriptional reprogramming during human osteoclast differentiation identifies regulators of osteoclast activity

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Bone Research 2024年第1期12卷 180-198页

作者： morten S.Hansen Kaja madsen maria Price Kent Søe Yasunori Omata mario m.Zaiss Caroline m.Gorvin morten Frost alexander Rauch molecular Endocrinology Laboratory(KmEB) Department of EndocrinologyOdense University HospitalDK-5000 Odense CDenmark Department of Clinical Research Faculty of Health SciencesUniversity of Southern DenmarkDK-5000 Odense CDenmark Clinical Cell Biology Pathology Research UnitDepartment of Clinical ResearchUniversity of Southern DenmarkDK-5000 Odense CDenmark Institute of metabolism and Systems Research(ImSR)and Centre for Diabetes Endocrinology and Metabolism(CEDAM)University of BirminghamBirmingham B152TTUK Centre for membrane Proteins and Receptors(COmPARE) Universities of Birmingham and NottinghamBirmingham B152TTUK Department of molecular medicine University of Southern DenmarkDK-5000 Odense CDenmark Department of Orthopedic Surgery Faculty of MedicineThe University of TokyoTokyo 113-8655Japan Department of Internal medicine 3 Rheumatology and ImmunologyFriedrich-Alexander-University Erlangen-Nürnberg(FAU)and Universitätsklinikum ErlangenD-91054 ErlangenGermany Deutsches Zentrum Immuntherapie(DZI) Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum ErlangenD-91054 ErlangenGermany Steno Diabetes Center Odense Odense University HospitalDK-5000 Odense CDenmark

Enhanced osteoclastogenesis and osteoclast activity contribute to the development of osteoporosis,which is characterized by increased bone resorption and inadequate bone formation.As novel antiosteoporotic therapeutics are needed,understanding the genetic regulation of human osteoclastogenesis could help identify potential treatment targets.This study aimed to provide an overview of transcriptional reprogramming during human osteoclast differentiation.Osteoclasts were differentiated from CD14+monocytes from eight female donors.RNA sequencing during differentiation revealed 8980 differentially expressed genes grouped into eight temporal patterns conserved across donors.These patterns revealed distinct molecular functions associated with postmenopausal osteoporosis susceptibility genes based on RNA from iliac crest biopsies and bone mineral density SNPs.Network analyses revealed mutual dependencies between temporal expression patterns and provided insight into subtype-specific transcriptional networks.The donor-specific expression patterns revealed genes at the monocyte stage,such as filamin B(FLNB)and oxidized low-density lipoprotein receptor 1(OLR1,encoding LOX-1),that are predictive of the resorptive activity of mature osteoclasts.The expression of differentially expressed G-protein coupled receptors was strong during osteoclast differentiation,and these receptors are associated with bone mineral density SNPs,suggesting that they play a pivotal role in osteoclast differentiation and activity.The regulatory effects of three differentially expressed G-protein coupled receptors were exemplified by in vitro pharmacological modulation of complement 5 A receptor 1(C5AR1),somatostatin receptor 2(SSTR2),and free fatty acid receptor 4(FFAR4/GPR120).Activating C5AR1 enhanced osteoclast formation,while activating SSTR2 decreased the resorptive activity of mature osteoclasts,and activating FFAR4 decreased both the number and resorptive activity of mature osteoclasts.In conclusion,we report the occurrence of transcriptional

关键词： osteoclast programming identif

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涂层中任意奇异点的弹性基本解研究

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中国科技论文 2016年第5期11卷 483-493页

作者：马利锋赵晶 alexander m.korsunsky 西安交通大学航天航空学院西安710049 牛津大学工程科学系

在涂层与基体分别为不同材料的涂层-基体体系中,涂层材料内存在一任意奇异点,运用格林函数法,推导得到这个问题的一般弹性基础解,运用几个退化模型证明基本解的正确性,并用三个简单的例子演示了此基本解的应用。一般任意奇异点可以是位... 详细信息

在涂层与基体分别为不同材料的涂层-基体体系中,涂层材料内存在一任意奇异点,运用格林函数法,推导得到这个问题的一般弹性基础解,运用几个退化模型证明基本解的正确性,并用三个简单的例子演示了此基本解的应用。一般任意奇异点可以是位错、点力、点距、点应变等,所以这个基本解是一系列不同奇异点弹性基本解的一般形式。这个广义的基本解可以作为格林函数法中的核函数用来求解其他更多更复杂的涂层基体问题,例如界面裂纹、TGO残余应变、材料不匹配、夹杂及热膨胀问题、相变问题等。

关键词：涂层-基体体系奇异解弹性基本解格林函数

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Praseodymium metal nanorods as a 5-fluorouracil carrier

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Journal of Rare Earths 2024年第7期42卷 1328-1336页

作者： Varnitha manikantan Govindaraj Sri Varalakshmi Ujma Ashapak Tamboli Archana Sumohan Pillai Aleyamma alexander Anushka Lucas Bose Allben Akash Israel V.m.V.Enoch Centre for Nanoscience and Genomics Karunya Institute of Technology and Sciences (Deemed University) Department of Applied Chemistry Karunya Institute of Technology and Sciences (Deemed University)

Interest in metal nanoparticles as nanoscale drug-carrying vehicles has been increasing.In such an aspect,lanthanide metal nanoparticles are particularly intriguing.Herein,we report praseodymium nanoparticles as a novel anticancer therapeutic transporting agent.The material was made as a composite with the supramolecular host polymer,poly-β-cyclodextrin.The praseodymium nanorods were characterized using standard analytical techniques,including X-ray diffraction（XRD）,X-ray photoelectron spectroscopy（XPS）,and thermogravimetric analysis（TGA）.In addition,they were imaged using transmission electron microscopy.The nanostructures are a long rod-shaped material with 2.0±0.1 μm in length and 230±10 nm in width.The material shows a saturation magnetization value above 30 emu/g with a coercivity value of 130 Oe.The nanocarrier is freighted with the drug 5-fluorouracil,and the encapsulation efficiency is 76.09%.The drug release is continuous from the nanocarrier（polymer-containing praseodymium nanoparticles） and proceeds for above 250 h.The in vitro cytotoxicities of the blank nanocarrier and the 5-fluorouracil-loaded one are deduced by testing them on mCF-7 and Vero（non-cancerous） cell lines.The cytotoxicity on the cancerous cell lines is observed,whereas the noncancerous cells are unaffected.The IC50values on the cell lines are reported.

关键词： Anticancer activity Drug delivery metal nanoparticles Poly-cyclodextrin Praseodymium Rare earths

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Effects of site preparation methods on soil physical properties and outplanting success of coniferous seedlings in boreal forests

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Journal of Forestry Research 2024年第2期35卷 70-80页

作者： Aleksey S.Ilintsev Elena N.Nakvasina alexander P.Bogdanov Northern Research Institute of Forestry 13 Nikitova Str.Arkhangelsk 163062Russian Federation Department of Silviculture and Forest management Northern(Arctic)Federal University Named After M.V.LomonosovSevernoy Dviny Emb.17Arkhangelsk 163002Russian Federation

This study assessed the effect of patch scarification and mounding on the physical properties of the root layer and the success of tree planting in various types of forests.This study was conducted on 12 forest sites in taiga forests of the European part of Russia.A total of 54 plots were set up to assess seedling survival;root collar diameter,height,and heigh increment were measured for 240 seedlings to assess growth.In the rooting layer,240 soil samples were taken to determine physical properties.The study showed that soil treatment methods had no effect on bulk density and total porosity in Cladina sites.However,reduced soil moisture was noted,particularly in mounds,resulting in increased aeration.In myrtillus sites,there were increased bulk density,reduced soil moisture,and total porosity in the mounds.mounding treatment in Polytrichum sites resulted in reduced soil moisture and increased aeration porosity.In the myrtillus and Polytrichum sites,patch scarification had no effects on physical properties.In Polytrichum sites,survival rates,heights,and heigh increments of bareroot Norway spruce seedlings in mounds were higher than in patches;however,the same did not apply to diameter.In Cladina and myrtillus sites,there was no difference in growth for bareroot and containerised seedlings with different soil treatments.Growing conditions and soil types should be considered when applying different soil treatment methods to ensure high survival rates and successful seedling growth.

关键词： Boreal forests mechanical site preparation Patch scarification mounding Soil properties Containerised seedlings Bareroot seedlings

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A Statistical Study of magnetic Flux Emergence in Solar Active Regions Prior to Strongest Flares

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Research in Astronomy and Astrophysics 2024年第4期24卷 147-160页

作者： alexander S.Kutsenko Valentina I.Abramenko Andrei A.Plotnikov Crimean Astrophysical Observatory p/o NauchnyCrimea298409Russia

Using the data on magnetic field maps and continuum intensity for Solar Cycles 23 and 24,we explored 100 active regions(ARs)that produced m5.0 or stronger flares.We focus on the presence/absence of the emergence of magnetic flux in these ARs 2-3 days before the strong flare onset.We found that 29 ARs in the sample emerged monotonically amidst quiet-Sun.A major emergence of a new magnetic flux within a pre-existing AR yielding the formation of a complex flare-productive configuration was observed in another 24 cases.For 30 ARs,an insignificant(in terms of the total magnetic flux of pre-existing AR)emergence of a new magnetic flux within the pre-existing magnetic configuration was observed;for some of them the emergence resulted in a formation of a configuration with a small δ-sunspot;11 out of 100 ARs exhibited no signatures of magnetic flux emergence during the entire interval of observation.In six cases the emergence was in progress when the AR appeared on the Eastern limb,so that the classification and timing of emergence were not possible.We conclude that the recent flux emergence is not a necessary and/or sufficient condition for strong flaring of an AR.The flux emergence rate of flare-productive ARs analyzed here was compared with that of flare-quiet ARs analyzed in our previous studies.We revealed that the flare-productive ARs tend to display faster emergence than the flare-quiet ones do.

关键词： Sun:activity Sun:flares Sun:magnetic fields

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Correction method for moderate and severe degrees of hallux valgus associated with transfer metatarsalgia

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World Journal of Orthopedics 2024年第3期15卷 238-246页

作者： Amangasy Zhanaspayev Nurlan Bokembayev marat Zhanaspayev Aidos Tlemissov Sabina Aubakirova alexander Prokazyuk Department of Traumatology and Orthopaedics The National Scientific Center of Traumatology and Orthopaedics Named after Academician Batpenov NDAstana 010000Kazakhstan Department of Traumatology and Paediatric Surgery Non-Commercial Joint-Stock Company“Semey Medical University”Semey 071400Kazakhstan Department of Habilitation and Rehabilitation Center of Habilitation and Rehabilitation of Persons with Disabilities of the Abai RegionSemey 071400Kazakhstan Department of Anaesthesiology and Resuscitation Center of Nuclear Medicine and OncologySemey 071400Kazakhstan

BACKGROUND Hallux valgus(HV)is a common foot deformity that manifests with increasing age,especially in women.The associated foot pain causes impaired gait and decreases quality of life.moderate and severe HV is a deformity that is charac-terized by the involvement of lesser rays and requires complex surgical treatment.In this study,we attempted to develop a procedure for this condition.AIm To analyse the treatment results of patients who underwent simultaneous surgical correction of all parts of a static forefoot deformity.mETHODS We conducted a prospective clinical trial between 2016 and 2021 in which 30 feet with moderate or severe HV associated with Tailor’s bunion and metatarsalgia were surgically treated via a new method involving surgical correction of all associated problems.This method included a modified Lapidus procedure,m2m3 tarsometatarsal arthrodesis,intermetatarsal fusion of the m4 and m5 bases,and the use of an original external fixation apparatus to enhance correction power.Preoperative,postoperative,and final follow-up radiographic data and American Orthopaedic Foot and Ankle Society(AOFAS)scores were compared,and P valuesmales(93.3%)and 2 males feet(6.7%),20(66.7%)of whom had a moderate degree of HV and 10(33.3%)of whom had severe deformity.m2 and m3 metatarsalgia was observed in 21 feet,and 9 feet experienced pain only at m2.The mean follow-up duration was 11 months.All patients had good correction of the HV angle[preoperative median,36.5 degrees,interquartile range(IQR):30-45;postoperative median,10 degrees,IQR:8.8-10;follow-up median,11.5 degrees,IQR:10-14;Pmetatarsalgia was resolved in most patients(30 vs 5).There was a clinically negligible decrease in the corrected angles at the final follow-up,and the overall AOFAS score was significantly better(median,65 points,IQR:53.8-70;vs 80 points,IQR:75-85;Pmethod showed good sustainability of correction po

关键词： Hallux valgus metatarsalgia Tailor’s bunion Lapidus procedure Proximal metatarsal osteotomy Splayfoot

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Impact of doping profiles on the formation of laser-active centers in bismuth-doped GeO_(2)–SiO_(2)glass fibers

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Photonics Research 2024年第2期12卷 260-270页

作者： SERGEY ALYSHEV alexander VAKHRUSHEV ALEKSANDR KHEGAI ELENA FIRSTOVA KONSTANTIN RIUmKIN mIKHAIL mELKUmOV LYUDmILA ISKHAKOVA ANDREY UmNIKOV SERGEI FIRSTOV Prokhorov General Physics Institute of the Russian Academy of Sciences Dianov Fiber Optics Research Center119333 MoscowRussia Devyatykh Institute of Chemistry of High-Purity Substances of the Russian Academy of Sciences 603951 Nizhny NovgorodRussia

multi-wavelength-band transmission technology based on the exploitation of the extended spectral region is considered as a potential approach to increase the transmission capacity in the deployed fiber-optic communication infrastructure.The development of optical amplifiers operating in the O-,E-,S-,and U-telecom bands is an extremely important challenge for the successful implementation of this technology.Bismuth-doped fibers are of increasing interest as gain materials,which can be used to provide broadband amplification in the mentioned telecom bands.This is due to the ability of Bi ions incorporated into glass network to form bismuth active centers(BACs)with specific optical properties,which are primarily determined by the glass modifiers.In this work,the impact of the doping profiles of both Ge atoms as glass modifiers and Bi ions on the BACs formation is studied using a series of bismuth-doped fibers fabricated by the modified chemical vapor deposition(mCVD)technique.The Bi-to-BACs conversion efficiency in various spatial regions of the studied samples is presented.It is turned out that for high-Bi concentration regions,the conversion efficiency is very low(less than 10%).In addition,the relationship of the conversion efficiency to the distribution of Bi ions and/or Ge atoms is discussed.Finally,a continuous-wave laser at 1.46μm with a record slope efficiency of 80%is demonstrated using a Bi-doped fiber with confined doping profile,where the Bi-to-BACs conversion efficiency is 35%.This paper provides new information which might help to facilitate understanding of the features of Bi-doped fibers and their potentially achievable characteristics.

关键词： fiber fibers bismuth

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