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Evaluation of photochemistry reaction kinetics to pattern bioactive proteins on hydrogels for biological applications

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Bioactive Materials 2018年第1期3卷 64-73页

作者： Taylor B.Dorsey alexander grath Annling Wang Cancan Xu Yi Hong Guohao Dai Department of Biomedical Engineering Rensselaer Polytechnic InstituteTroyNY 12180USA Center for Biotechnology and Interdisciplinary Studies Rensselaer Polytechnic InstituteTroyNY 12180USA Department of Bioengineering Northeastern UniversityBostonMA 02115USA Department of Bioengineering University of Texas at ArlingtonArlingtonTX 76019USA

Bioactive signals play many important roles on cell function and *** most biological studies,soluble biochemical cues such as growth factors or cytokines are added directly into the media to maintain and/or manipulate cell activities in ***,these methods cannot accurately mimic certain in vivo biological signaling motifs,which are often immobilized to extracellular matrix and also display spatial gradients that are critical for tissue *** biochemical cues,biophysical properties such as substrate stiffness can influence cell behavior but is not easy to manipulate under conventional cell culturing *** development in photocrosslinkable hydrogels provides new tools that allow precise control of spatial biochemical and biophysical cues for biological applications,but doing so requires a comprehensive study on various hydrogel photochemistry kinetics to allow thorough photocrosslink reaction while maintain protein bioactivities at the same *** this paper,we studied several photochemistry reactions and evaluate key photochemical parameters,such as photoinitiators and ultra-violet(UV)exposure times,to understand their unique contributions to undesired protein damage and cell *** data illustrates the retention of protein function and minimize of cell health during photoreactions requires careful selection of photoinitiator type and concentration,and UV exposure *** also developed a robust method based on thiol-norbornene chemistry for independent control of hydrogel stiffness and spatial bioactive ***,we highlight a class of bioactive hydrogels to stiffness control and site specific immobilized bioactive proteins/peptides for the study of cellular behavior such as cellular attraction,repulsion and stem cell fate.

关键词： Photo-patterning Bioactive signals PEG hydrogels Click-chemistry

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