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External quality control and training of semen analysis in the Netherlands:starting point for further reduction of outcome variability

引用

Asian Journal of Andrology 2022年第1期24卷 15-20页

作者： Louise Lemmens Leonie van den Hoven Netty J van Vrouwerff Didi DM Braat Willianne LDM Nelen Marian A Spath alex mm wetzels Fertility Laboratory Department of Obstetrics and GynaecologyRadboud University Medical CenterPO Box 91016500 HB NijmegenThe Netherlands Department of Clinical Chemistry Sint Antonius HospitalPO Box 25003430 EM NieuwegeinThe Netherlands Department of Obstetrics and Gynaecology Radboud University Medical CenterPO Box 91016500 HB NijmegenThe Netherlands

Semen analysis is characterized by high levels of intra-and inter-laboratory variability,due to a low level of standardization,high subjectivity of the assessments,and problems with automated *** improve consistency of laboratory results,quality control and training of technicians are important *** goals of this study are to evaluate the results of an external quality control(EQC)program and standardized training by ESHRE Basic Semen Analysis Courses(BSAC)on the variability in manual assessments of semen *** performed retrospective analyses of(1)the interlaboratory variability in the Dutch EQC program and(2)the interobserver variability in BSACs for concentration,motility,and morphology *** data showed that the interlaboratory coefficient of variation(CV)for concentration assessment decreased(range from 24.0%–97.5%to 12.7%–20.9%)but not for morphology and motility *** variability was lower if improved Neubauer hemocytometers were *** assessment showed highest CVs(up to 375.0%),with many outliers in the period of 2007–*** BSAC,a significant reduction of interobserver variability could be established for all parameters(P<0.05).The absence of an effect in the EQC program for motility and morphology might be explained by respectively the facts that motility assessment was introduced relatively late in the EQC program(since 2013)and that criteria for morphology assessment changed in *** results might have been influenced by the pretraining level of participants and the influence of external *** EQC and training show positive effects on reducing *** willingness by laboratories to change their methods toward standards may lead to further improvements.

关键词： external quality control semen analysis standardized training

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