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Cell-specific pathways recruited for symbiotic nodulation in the Medicago truncatula legume

Molecular plant 2022年第12期15卷 1868-1888页

作者： Sergio Alan Cervantes-pérez Sandra Thibivilliers Carole Laffont Andrew D.Farmer Florian Frugier Marc Libault Department of Agronomy and Horticulture Center for Plant Science InnovationUniversity of Nebraska-LincolnLincolnNE 68503USA Single Cell Genomics Core Facility Center for BiotechnologyUniversity of Nebraska-LincolnLincolnNE 68588USA Institute of plant Sciences paris-Saclay(IpS2) UniversitéParis-SaclayCNRSINRAEUniversitéParis-CitéUniversitéd’Evry91190 Gif-sur-YvetteFrance National Center for Genome Resources Santa FeNM 87505USA

Medicago truncatula is a model legume species that has been studied for decades to understand the symbiotic relationship between legumes and soil bacteria collectively named *** symbiosis called nodulation is initiated in roots with the infection of root hair cells by the bacteria,as well as the initiation of nodule primordia from root cortical,endodermal,and pericycle cells,leading to the development of a new root organ,the nodule,where bacteria fix and assimilate the atmospheric dinitrogen for the benefit of the ***,we report the isolation and use of the nuclei from mock and rhizobia-inoculated roots for the single nuclei RNA-seq(sNucRNA-seq)profiling to gain a deeper understanding of early responses to rhizobial infection in Medicago roots.A gene expression map of the Medicago root was generated,comprising 25 clusters,which were annotated as specific cell types using 119 Medicago marker genes and orthologs to Arabidopsis cell-type marker genes.A focus on root hair,cortex,endodermis,and pericycle cell types,showing the strongest differential regulation in response to a short-term(48 h)rhizobium inoculation,revealed not only known genes and functional pathways,validating the sNucRNA-seq approach,but also numerous novel genes and pathways,allowing a comprehensive analysis of early root symbiotic responses at a cell type-specific level.

关键词： Medicago root single-cell transcriptomic rhizobium nodule initiation root hair cells cortical cells

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Cadmium and lead effects on chlorophyll fluorescence,chlorophyll pigments and proline of Robinia pseudoacacia

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Journal of Forestry Research 2015年第2期26卷 323-329页

作者： A.Dezhban A.Shirvany p.Attarod M.Delshad M.Matinizadeh M.Khoshnevis Department of Forestry and Forest Economics Faculty of Natural ResourcesUniversity of Tehran Department of Horticultural Sciences Faculty of Agricultural Sciences and EngineeringUniversity of Tehran Research Institute of Forests and Rangelands

Heavy metal contamination is one of the most important abiotic stresses affecting physiological activities of *** investigated the effects of cadmium（Cd） and lead（pb） on chlorophyll fluorescence（Fv/Fm,Fo,and Fm）,photosynthetic pigments（chlorophyll a and b）,and proline in one-year-old seedlings of Robinia *** seedlings were treated twice over a period of 10 days with Cd and pb at concentrations of 0,250,500,1000 and2000 mg *** solution containing Cd and pb was sprayed on the *** and proline contents were measured after 10 *** fluorescence of *** was affected slightly by high concentrations（1000,2000 mg L-1） of Cd and *** a and a/b increased at 1000 and 2000 mg L-1of Cd and proline content of leaves was similar in all treatments of Cd and *** results indicated that photosynthetic sensitivity of *** to Cd and pb contamination was *** II chlorophyll pigments were not damaged by pb and Cd *** conclude that chlorophyll fluorescence along with chlorophyll and proline contents are useful indicators of Cd and pb stresses in *** which widely planted in urban polluted regions in Iran.

关键词： Robinia pseudoacacia Cadmium Lead chlorophyll fluorescence chlorophyll proline

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Quasiperiodic Motions for Asymmetric Oscillators

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Acta Mathematica Sinica,English Series 2001年第2期17卷 253-262页

作者： Xiao ping YUAN Department of Mathematics. Fudan University. Shanghai 200433, p. R. china Department of Mathematics Fudan University Shanghai 200433 P. R. China

We prove the existence of quasiperiodic solutions and Lagrange stability for a class of differential equations with jumping nonlinearity x+ax^+-bx^-+φ(x)=p(t), where a, b>0, p(t)∈ C(R/2πZ) and φ: R→R is an unboun... 详细信息

关键词： Quasiperiodic solution Lagrange stability KAM theorem

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Vascular plants distribution in relation to topography and environmental variables in alpine zone of Kedarnath Wild Life Sanctuary,West Himalaya

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Journal of Mountain Science 2018年第9期15卷 1936-1949页

作者： chANDRA Sudeep SINGH Ankit SINGH C.p. NAUTIYAL M.C. RAWAT L.S. High Altitude plant physiology Research Centre(HAppRC)H.N.B.Garhwal University Srinagar Garhwal-246174UttarakhandIndia Space Applications Centre (Indian Space Research Organization)Ahmedabad 380015India Govind Ballabh pant National Institute of Himalayan Environment&Sustainable Development Garhwal Unit Srinagar Garhwal-246174UttarakhandIndia

The present study was carried out in Tungnath alpine meadows of Kedarnath Wild Life Sanctuary, Western Himalaya from subalpine to upper alpine zone. A total of four summits were selected along an altitudinal gradient and sampled for detailed vegetation analysis using multi summit approach as per Global observation research initiative in alpine environments(GLORIA). Species richness, diversity, and evenness among four summits as well as the interaction between environmental variables with plant communities were assessed. Monthly mean soil temperature was calculated using data retrieved from geo-precision temperature logger in order to identify the trend of soil temperature among different season and altitudinal gradient and its implications to plant communities. Soil samples were analyzed fromeach summit by collecting randomized composite soil samples. The indirect non-metric multidimensional scaling(NMDS) and direct canonical correspondence analysis(CCA) tools of ordination techniques to determine the linkage between plant species from various sample summits and biotic/abiotic environmental gradients were used in the present study. The results of the study demonstrated increase in species richness as soil temperature increases, the ecotone representing summits were found most warm summits followed by highest species richness. Annual soil temperature increased by 1.43°C at timberline ecotone. Whereas, at upper alpine zone the soil temperature increased by 0.810 C from year 2015 to 2016. S?rensen's similarity index was found to be increased between subalpine and upper alpine zone with increase in the presence of subalpine plant species at upper alpine zone. Both the ordination tools separate the subalpine summit and their respective vegetation from summits representingtimberline ecotone and upper alpine zone. Soil p H, altitude, soil cation exchange capacity were found as the key abiotic drivers for distribution of plant species.

关键词： GLORIA Non-metric multidimensionalscaling (NMDS) Canonical correspondence analysis(CCA) Ecotone West Himalaya

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Development of artificial neural network to predict the performance of spark ignition engine fuelled with waste pomegranate ethanol blends

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Information processing in Agriculture 2023年第4期10卷 459-474页

作者： D.Y.Dhande C.S.choudhari D.p.Gaikwad Kiran B.Dahe Department of Mechanical Engineering AISSMS College of EngineeringPuneMaharashtra State 411001India Department of Computer Engineering AISSMS College of EngineeringPuneMaharashtra State 411001India

In this study,an artificial neural network(ANN)is developed to predict the performance of a spark-ignition engine using waste pomegranate ethanol blends.A series of experiments on a single-cylinder,four-stroke spark-ignition engine yielded the data needed for neural network training and validation.70 percent of the experimental data was used to train the network using the feed-forward back propagation(FFBp)*** developed network model’s performance was evaluated by contrasting its output with experimental *** parameters included engine speed,ethanol blends,and output parameters included indicated and brake power,thermal,volumetric,and mechanical *** and testing data had regression coefficients that were almost identical to *** research revealed that the ANN model can be a better option for predicting engine performance with a higher level of accuracy.

关键词： ANN Biofuel pomegranate ethanol Solid waste management Spark ignition engine performance

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Natural Bond Orbital(NBO)population Analysis,First Order Hyperpolarizabilities and Thermodynamic properties of Cyclohexanone

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光谱学与光谱分析 2015年第6期35卷 1506-1511页

作者： Rubarani p.Gangadharan S.Sampath Krishnan Department of physics Rajalakshmi Engineering College Department of Applied physics Sri Venkateswara College of Engineering

The molecular structure of cyclohexanone was calculated by the B3LYp density functional model with 6-31G(d,p)and 6-311++G(d,p)basis set by Gaussian *** results from natural bond orbital(NBO)analysis have been analyzed in terms of the hybridization of atoms and the electronic structure of the title *** electron density based local reactivity descriptors such as Fukui functions were *** dipole moment(μ)and polarizability(α),anisotropy polarizability(Δα)and first order hyperpolarizability(βtot)of the molecule have been *** properties of the title compound were calculated at different temperatures.

关键词： Density functional theory(DFT) Natural Bond Orbital(NBO)Analysis Hyperpolarizability

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Geochemistry and petrogenesis of the paleoproterozoic orthogneisses and granitoids of the Banded Gneissic Complex,central Rajasthan,NW India:Implications for crustal reworking processes

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Acta Geochimica 2023年第2期42卷 373-386页

作者： Iftikhar Ahmad T.p.Abdul Latheef M.E.A.Mondal Ismail S.Hamidullah Kamaal parvez Department of Geology Aligarh Muslim UniversityAligarhU.P 202002India Department of Earth Sciences Indian Institute of Technology RoorkeeRoorkee 247667India

Granitoids and orthogneisses(1.7 Ga)from the northern part of the Banded Gneissic Complex(BGC-II domain),Aravalli Craton(NW India)are geochemically analyzed to understand the geodynamic condition and crustal evolution *** samples are metaluminous to peraluminous(molar A/CNK:0.74±2.12),and characterized by Eu-anomaly ranging from 0.17 to 1.06,Fe_(2)O_(3)^(T)/(Fe_(2)O_(3)^(T)+MgO)from 0.8 to 0.9 and high magmatic zircon saturation temperature(>830°C)which are the features suggestive of A-type granite *** discrimination diagrams classify the samples as post-collisional extensional A2-type *** characteristics along with trace element ratios[(Y/Nb)_(N)=0.15 to 4.33(avg.0.76),(Th/Nb)_(N)=4.63 to 255.47(avg.63.13),(Th/Ta)_(N)=1.37 to 9.84(avg.8.86),(Ce/pb)_(N)=0.05 to 3.05(avg.1.43)]indicate that the rocks were derived from a plagioclase-rich and garnet-free crustal source under lowpressure ***,it is also proposed that tonalite-trondhjemite-granodiorite(TTG)rocks which occur dominantly in southern Rajasthan(BGC-I)are precursors and their partial melting led to the generation of the studied A-type granite *** studied samples also bear close geochemical similarity with A-type granites of similar age(1.7 Ga)near the Sakhun-Ladera region of northern *** studied A-type granites are believed to be coeval to similar aged A-type granites of the Khetri and Alwar sub-basins of the North Delhi Fold Belt(NDFB).They are comparable in age and magmatic history to recorded A-type magmatism in North America and parts of the chinese *** large geographical extents of synchronous A-type granites are proposed to be related to the Columbia Supercontinent assembly(ca.1.7 Ga;postcollisional granites).Thus,based on the studied extensional granites,we surmise that BGC-II was part of the Columbia Supercontinent.

关键词： Banded Gneissic Complex(BGC II) Aravalli Craton A-type granites Orthogneisses Rifting of Columbia

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Archery Algorithm:A Novel Stochastic Optimization Algorithm for Solving Optimization problems

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Computers, Materials & Continua 2022年第7期72卷 399-416页

作者： Fatemeh Ahmadi Zeidabadi Mohammad Dehghani pavel Trojovsky Štěpán Hubálovsky Victor Leiva Gaurav Dhiman Department of Mathematics and Computer Sciences Sirjan University of TechnologySirjanIran Department of Mathematics Faculty of ScienceUniversity of Hradec Králové50003Hradec KrálovéCzech Republic Department of Applied Cybernetics Faculty of ScienceUniversity of Hradec Králové50003Hradec KrálovéCzech Republic School of Industrial Engineering Pontificia Universidad Católica de ValparaísoValparaíso2362807Chile Department of Computer Science Government Bikram College of CommercePatialaPunjabIndia

Finding a suitable solution to an optimization problem designed in science is a major ***,these must be addressed utilizing proper *** on a random search space,optimization algorithms can find acceptable solutions to *** Algorithm(AA)is a new stochastic approach for addressing optimization problems that is discussed in this *** fundamental idea of developing the suggested AA is to imitate the archer’s shooting behavior toward the target *** proposed algorithm updates the location of each member of the population in each dimension of the search space by a member randomly marked by the *** AA is mathematically described,and its capacity to solve optimization problems is evaluated on twenty-three distinct types of objective ***,the proposed algorithm’s performance is compared *** approaches,including teaching-learning based optimization,marine predators algorithm,genetic algorithm,grey wolf optimization,particle swarm optimization,whale optimization algorithm,gravitational search algorithm,and tunicate swarm *** to the simulation findings,the AA has a good capacity to tackle optimization issues in both unimodal and multimodal scenarios,and it can give adequate quasi-optimal solutions to these *** analysis and comparison of competing algorithms’performance with the proposed algorithm demonstrates the superiority and competitiveness of the AA.

关键词： Archer meta-heuristic algorithm population-based optimization stochastic programming swarm intelligence population-based algorithm Wilcoxon statistical test

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Molecular and physiological Analysis of Growth-Limiting Drought Stress in Brachypodium distachyon Leaves

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Molecular plant 2013年第2期6卷 311-322页

作者： Wim Verelst Edoardo Bertolini Stefanie De Bodt Klaas Vandepoele Marlies Demeulenaere Mario enrico pè Dirk Inzé Department of plant Systems Biology vie Technologiepark 927 9052 Gent Belgium Department of plant Biotechnology and Bioinformatics Ghent University Technologiepark 927 9052 Gent Belgium present address: Rijk Zwaan Zaadteelt en Zaadhandel B.V. Eerste Kruisweg 9 4793 RS Fijnaart The Netherlands Scuola Superiore Sant'Anna Piazza Martiri della Liberta 33 56127 Pisa Italy

The drought-tolerant grass Brachypodium distachyon is an emerging model species for temperate grasses and cereal crops. To explore the usefulness of this species for drought studies, a reproducible in vivo drought assay was developed. Spontaneous soil drying led to a 45% reduction in leaf size, and this was mostly due to a decrease in cell expansion, whereas cell division remained largely unaffected by drought. To investigate the molecular basis of the observed leaf growth reduction, the third Brachypodium leaf was dissected in three zones, namely proliferation, expan- sion, and mature zones, and subjected to transcriptome analysis, based on a whole-genome tiling array. This approach allowed us to highlight that transcriptome profiles of different developmental leaf zones respond differently to drought. Several genes and functional processes involved in drought tolerance were identified. The transcriptome data suggest an increased energy availability in the proliferation zones, along with an up-regulation of sterol synthesis that may influ-ence membrane fluidity. This information may be used to improve the tolerance of temperate cereals to drought, which is undoubtedly one of the major environmental challenges faced by agriculture today and in the near future.

关键词： Brachypodium drought stress plant growth leaf.

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THE TURBULENT MODEL OF LIQUID-pARTICLE FLOWS AND ITS AppLICATION TO NUMERICAL pREDICTION OF OIL pOLLUTION

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Journal of Hydrodynamics 1992年第2期4卷 72-78页

作者： Shen Yong-ming Zhao Wen-qian, Dalian University of Technology, Dalian 116023, p. R. china Dalian Univ of Technology Dalian China

Simulation of particle turbulent transport in present mathematical models of two- phase turbulent flows is algebraic model based on local following theory. The predicted results by these models are not consistent with that of experiments available. The motion of two-phase flow is described by a unified model of multi-fluid in this paper. The two-fluid turbulent model of two-phase flow based on k-e equations for particles and fluid, and corresponding numerical thch- nique are both developed. The proposed model and the numerical technique are successfully em- ployed to numerical modelling for oil-water two-phase turbulent recirculating flow.

关键词： liquid-particle flows particle turbulence oil pollution

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