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A Tool for Interrogation of Macromolecular Structure

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材料科学与工程（中英文B版） 2014年第2期4卷 55-63页

作者： Francisco Torrens gloria Castellano University Institute for Molecular Science University of Valencia Building of Institutes of Paterna P. O. Box 22085 Valencia46071 Spain Faculty of Veterinary and Experimental Sciences Valencia Catholic University Saint Vincent Martyr Guillem de Castro-94Valencia 46001 Spain

Binding of Mono, Bi and Tridomain Proteins to Zwitterionic and Anionic Vesicles： Asymmetric Location of Anionic Phospholipids in Mixed Zwitterionic/Anionic Vesicles and Cooperative Binding

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Journal of Life Sciences 2011年第3期5卷 167-181页

作者： Francisco Torrens gloria Castellano Institute for Molecular Science University of Valencia Valencia E-46071 Spain Energesis Chair of Interdisciplinary Technology Saint Vincent Martyr Catholic University of Valencia Valencia E-46003 Spain

Lysozyme, myoglobin and BSA were used as models of globular proteins covering a wide range of pl. The purpose is to extend the studies to anionic lipid bilayers. Electrostatics is studied in cationic protein adsorption to zwitterionic PC and anionic mixed PC/PG SUVs. Protein adsorption is investigated in SUVs along with changes of fluorescence emission spectra. Partition coefficients and cooperativity parameters are calculated. At pl binding obtains maximum while at lower or higher pHs binding decreases. In Gouy-Chapman formalism activity coefficient goes with square charge, which deviations indicate asymmetric location of anionic phospholipid in the inner leaflet, in mixed SUVs for lysozyme- and myoglobin-PC/PG systems, in agreement with experiments and molecular dynamics simulations. Vesicles bind myoglobin anti-cooperatively while lysozyme-BSA cooperativitivey. A model is proposed for both, which composes two protein sub-layers with different structures and properties. Hill coefficient reflects subunit cooperativity of bi and tridomain proteins.

关键词： Protein-lipid interaction sigmoid adsorption isotherm transbilayer asymmetry anionic phospholipid asymmetric location cooperative binding hill plot.

Manet and modern beauty

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Manet and modern beauty

C-Nanostructures Cluster Models in Organic Solvents： Fullerenes, Tubes, Buds and Graphenes

作者： edited by Scott Allan Emily A. Beeny gloria Groom

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Journal of Chemistry and Chemical Engineering 2013年第11期7卷 1026-1035页

作者： Francisco Torrens gloria Castellano University Institute for Molecular Science University of Valencia Valencia 46071 Spain Faculty of Veterinary and Experimental Sciences Valencia Catholic University Saint Vincent Martyr Valencia 46001 Spain

The existence of nanographene in cluster form is discussed in organic solvents. Theories are developed based on the columnlet, bundlet and droplet models describing the size-distribution functions. Phenomena present a unified explanation in the columnlet model in which free energy of Cgraphene involved in cluster is combined from a volume part proportional to the number of molecules n in cluster and a constant. The columnlet model enables describing distribution function of Cgraphene clusters by size. From purely geometrical considerations the columnlet （Cgraphene）, bundlet （single-wall carbon nanotube）, CNT （carbon nanotube）, SWNT （single-wall C-nanotube）, and carbon nanobud, CNB （carbon nanobud）） and droplet （fullerene） models predict dissimilar behaviours. The interaction-energy parameters of Cgraphene are taken from C60. An CNB behaviour or further is expected. The decay of solubility with rising temperature is smaller for Cgraphene than for SWNT and CNB and, furthermore, than for C60, in agreement with lesser numbers of units in Cgraphene clusters. The discrepancy between the experimental data of the heat of solution of fullerenes, CNTs, CNBs and graphenes is ascribed to the sharp concentration dependence of the heat of solution. The diffusion coefficient drops with temperature result greater for Cgraphene than CNB and SWNT than C60 corresponding to lesser number of units in clusters. The aggregates near （C60）13, SWNT/CNB7 and （Cgraphene）3 could be representative of the droplet, bundlet and columnlet models.

关键词： Nanostructure graphene solubility columnlet cluster model bundlet cluster model droplet cluster model nanobud.

Towards efficient strain engineering of 2D materials:A four-points bending approach for compressive strain

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Nano Research 2024年第6期17卷 5317-5325页

作者： Hao Li Félix Carrascoso Ana Borrás gloria P.Moreno Francisco J.Aparicio Ángel Barranco Andrés Castellanos Gómez Campus de Cantoblanco Instituto de Ciencia de Materiales de MadridC.Sor Juana Inés de la Cruz328049 MadridSpain Nanotechnology on Surfaces and Plasma Lab Instituto de Ciencia de Materiales de Sevilla(CSIC-Universidad de Sevilla).C/Americo Vespucio 49E-41092 SevilleSpain Departamento de Física Aplicada I Escuela Politécnica SuperiorUniversidad de Sevillac/Virgen deÁfrica 7E-41011 SevilleSpain

Strain engineering,as a powerful strategy to tune the optical and electrical properties of two-dimensional(2D)materials by deforming their crystal lattice,has attracted significant interest in recent years.2D materials can sustain ultra-high strains,even up to 10%,due to the lack of dangling bonds on their surface,making them ideal brittle *** remarkable mechanical resilience,together with a strong strain-tunable band structure,endows 2D materials with a broad optical and electrical response upon ***,strain engineering based on 2D materials is restricted by their nanoscale and strain quantification *** this study,we have modified a homebuilt three-points bending apparatus to transform it into a four-points bending apparatus that allows for the application of both compressive and tensile strains on 2D *** approach allows for the efficient and reproducible construction of a strain system and minimizes the buckling effect caused by the van der Waals interaction by adamantane encapsulation *** results demonstrate the feasibility of introducing compressive strain on 2D materials and the potential for tuning their optical and physical properties through this approach.

关键词： uniaxial compressive strain polymer encapsulation differential reflectance spectroscopy exciton tunability

Association of swine influenza H1N1 pandemic virus(SIV-H1N1p) with porcine respiratory disease complex in sows from commercial pig farms in Colombia

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Virologica Sinica 2014年第4期29卷 242-249页

作者： Luisa Fernanda Mancipe Jiménez gloria Ramírez Nieto Victor Vera Alfonso Jairo Jaime Correa Laboratory of Animal Virology Faculty of Veterinary Medicine and Animal Science National University of Colombia

Porcine respiratory disease complex （PRDC） is a serious health problem that mainly affects growing and finishing pigs. PRDC is caused by a combination of viral and bacterial agents, such as porcine reproductive and respiratory syndrome virus （PRRSV）, swine influenza virus （SIV）, Mycoplasma hyopneumoniae （Myh）, Actinobacillus pleuropneumoniae （APP）, Pasteurella multocida and Porcine circovirus 2 （PCV2）. To characterize the specific role of swine influenza virus in PRDC presentation in Colombia, 11 farms from three major production regions in Colombia were examined in this study. Nasal swabs, bronchial lavage and lung tissue samples were obtained from animals displaying symptoms compatible with SIV. Isolation of SIV was performed in 9-day embryonated chicken eggs or Madin-Darby Canine Kidney （MDCK） cells. Positive isolates, identified via the hemagglutination inhibition test, were further analyzed using PCR. Overall, 7 of the 11 farms were positive for SIV. Notably, sequencing of the gene encoding the hemagglutinin （HA） protein led to grouping of strains into circulating viruses identified during the human outbreak of 2009, classified as pandemic H1N1-2009. Serum samples from 198 gilts and multiparous sows between 2008 and 2009 were obtained to determine antibody presence of APP, Myh, PCV2 and PRRSV in both SIV-H1Nlp-negative and -positive farms, but higher levels were recorded for SIV- HI Nlp-positive farms. Odds ratio （OR） and P values revealed statistically significant differences （p〈0.05） in PRDC presentation in gilts and multiparous sows of farms positive for SIV-HINlp. Our findings indicate that positive farms have increased risk of PRDC presentation, in particular, PCV2, APP and Myh.

关键词： SIV-HIN1 swine influenza pandemic porcine respiratory disease complex Colombia

Lay vs. Professional Perspectives: The Sexual and Reproductive Health of Orphans and Vulnerable Children in Uganda

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Open Journal of Preventive Medicine 2014年第7期4卷 573-582页

作者： gloria K. Seruwagi Department of Public Health Victoria University Kampala Uganda

Background/Introduction: Orphans and vulnerable children (OVC) in sub-Saharan Africa have been the focus of much popular and academic writing and the growing body of evidence cannot be overlooked. Existing research focuses on projections of OVC numbers, interventions, outcomes and descriptions of care arrangements. A lot of information exists on their poor sexual and reproductive health (SRH) status—most of it quantitative. The purpose of this paper is to present the voice and perceptions of OVC in regards to their SRH. Methods: This was a mixed-methods qualitative study drawing from phenomenology and it used purposive sampling to recruit 129 participants. Results: OVC perceptions and priorities do not always intersect with those of professionals and this has implications for existing and planned interventions. For example some OVC think favorably about early marriages while others have mechanisms of self-protection again SRH harm although this is not usually acknowledged. Conclusions: OVC demonstrate agency;therefore positioning them at the heart of service planning and delivery is an effective strategy in increasing the uptake and impact of interventions seeking to address their sexual and reproductive health needs.

关键词： Orphans and Vulnerable Children OVC OVC Health Child Health Child Agency

Comparison between Liver Cancer Mortality Rates in China and Spain

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Journal of Clinical and Translational Hepatology 2024年第10期12卷 831-833页

作者： Javier Guinea-Castanares Jesus Maria Iturralde Iriso Irune Natalia Elizondo Pinillos gloria Martinez Iniesta Osakidetza Basque Health Service Ringgold Standard Institution(16651) Vitoria-GasteizSpain

Dear Editors,We have read with interest the article by Liu H et al.1 published in your journal regarding liver cancer mortality in China and would like to compare it with that of our country,Spain,which is a European ... 详细信息

关键词： mortality Cancer

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