检索结果-南通市图书馆

Effects of calibration process on the simulation of rear-end conflicts at roundabouts

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Traffic and Transportation Engineering(English Edition) 2019年第2期6卷 175-184页

作者： Vincenzo Gallelli Giuseppe Guido Alessandro Vitale Rosolino Vaiana Department of Civil Engineering University of Calabria

A methodology for calibrating and validating VISSIM simulation model is presented that allows to replicate the observed vehicles conflicts. A roundabout case study has been selected to test the usefulness of a combined approach of VISSIM simulation package and the surrogate safety assessment model(SSAM) for providing reliable estimates of traffic conflicts. Safety performance has been assessed from the field by video-recording vehicle interactions at the roundabout, and then expressed in terms of time to collision(TTC)values.The proposed calibration procedure has been performed by a multistage methodology involving microscopic drivers' car following behavior parameters to enhance the correlation between observed and simulated queue lengths at the roundabout's entries. The calibration procedure is based on a statistical screening of inputs leading to a linear expression relating significant parameters to the queue length. The best estimates of the model's parameters have been determined using a genetic algorithm technique.The spatial distribution of the rear-end conflicts and the TTC values determined by SSAM have been finally compared with the observed ones to analyze the capability of the model of replicating rear-end conflicts.The results suggest to this calibration procedure impacts positively on the estimate of the safety performance measures obtained through the simulation processes.Notwithstanding the good results in the evaluation of the model's accuracy, the simulation seems to fail in reproducing the traffic phenomena linked to unusual driving behavior, and therefore it is not able to replicate forced drivers' maneuvers that can lead to a conflict situation.

关键词： Simulation Safety performance Unmanned aerial vehicle Roundabouts Traffic conflicts

Transplant benefit for patients with hepatocellular carcinoma

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2013年第48期19卷 9183-9188页

作者： Alessandro Vitale Michael Volk Umberto Cillo Unità di Chirurgia Epatobiliare e Trapianto Epatico Azienda Ospedaliera-Università di Padova 35128 Padova Italy Division of Gastroenterology and Hepatology University of Michigan Ann Arbor MI 48109 United States

Although liver transplantation is theoretically the best treatment for hepatocellular carcinoma(HCC),it is limited by the realities of perioperative complications,and the shortage of donor organs.Furthermore,in many cases there are available alternative treatments such as resection or locoregional therapy.Deciding upon the best option for a patient with HCC is complicated,involving numerous ethical principles including:urgency,utility,intention-to-treat survival,transplant benefit,harm to candidates on waiting list,and harm to living donors.The potential contrast between different principles is particularly relevant for patients with HCC for several reasons:(1)HCC candidates to liver transplantation are increasing;(2)the great prognostic heterogeneity within the HCC population;(3)in HCC patients tumor progression before liver transplantation may significantly impair post transplant outcome;and(4)effective alternative therapies are often available for HCC candidates to liver transplantation.In this paper we suggest that allocating organs by transplant benefit could help balance these competing principles,and also introduce equity between patients with HCC and nonmalignant liver disease.We also propose a triangular equipoise model to help decide between deceased donor liver transplantation,living donor liver transplantation,or alternative therapies.

关键词： Hepatocellular carcinoma Deceased donor liver transplantation Living donor liver transplantation Transplant benefit Utility Urgency Intention-to-treat survival Harm

Molecular Mechanism of the Specificity of Protein Import into Chloroplasts and Mitochondria in Plant Cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2019年第7期12卷 951-966页

作者： Dong Wook Lee Sumin Lee Junho Lee Seungjin Woo Md.Abdur Razzak Alessandro Vitale Inhwan Hwang Division of Integrative Biosciences and Biotechnology Pohang University of Science and Technology Pohang 37673 Korea Department of Life Sciences Pohang University of Science and Technology Pohang 37673 Korea Istituto di Biologia e Biotecnologia Agraria Consiglio Nazionale Delle Ricerche Milano Italy

Plants possess both types of endosymbiotic organelles, chloroplasts and mitochondria. Transit peptides and presequences function as signal sequences for specific import into chloroplasts and mitochondria, respectively. However, how these highly similar signal sequences confer the protein import specificity remains elusive. Here, we show that mitochondrial- or chloroplast-specific import involves two distinct steps, specificity determination and translocation across envelopes, which are mediated by the N-terminal regions and functionally interchangeable C-terminal regions, respectively, of transit peptides and presequences. A domain harboring multiple-arginine and hydrophobic sequence motifs in the N-terminal regions of presequences was identified as the mitochondrial specificity factor. The presence of this domain and the absence of arginine residues in the N-terminal regions of otherwise common targeting signals confers specificity of protein import into mitochondria and chloroplasts, respectively. AtToc159, a chloroplast import receptor, also contributes to determining chloroplast import specificity. We propose that common ancestral sequences were functionalized into mitochondrial- and chloroplast-specific signal sequences by the presence and absence, respectively, of multiple-arginine and hydrophobic sequence motifs in the N-terminal region.

关键词： transit peptide presequence protein import into chloroplasts and mitochondria N-terminal specificity domain C-terminal common translocation domain import specificity determination

Prediction of hepatocellular carcinoma biological behaviorin patient selection for liver transplantation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2016年第1期22卷 232-252页

作者： Umberto Cillo Tommaso Giuliani Marina Polacco Luz Maria Herrero Manley Gino Crivellari Alessandro Vitale Hepatobiliary Surgery and Liver Transplantation Unit Department of General Surgery and Organ TransplantationUniversity Hospital of Padua35128 PadovaItaly UnitàOperativa di Oncologia Medica Istituto Oncologico Veneto(IOV)IRCCS35128 PadovaItaly

Morphological criteria have always been considered the benchmark for selecting hepatocellular carcinoma(HCC)patients for liver transplantation(LT).These criteria,which are often inappropriate to express the tumor’s biological behavior and aggressiveness,offer only a static view of the disease burden and are frequently unable to correctly stratify the tumor recurrence risk after LT.Alpha-fetoprotein(AFP)and its progression as well as AFP-m RNA,AFP-L3%,des-γ-carboxyprothrombin,inflammatory markers and other serological tests appear to be correlated with post-transplant outcomes.Several other markers for patient selection including functional imaging studies such as18F-FDG-PET imaging,histological evaluation of tumor grade,tissue-specific biomarkers,and molecular signatures have been outlined in the literature.HCC growth rate and response to pre-transplant therapies can further contribute to the transplant evaluation process of HCC patients.While AFP,its progression,and HCC response to pretransplant therapy have already been used as a part of an integrated prognostic model for selecting patients,the utility of other markers in the transplant setting is still under investigation.This article intends to review the data in the literature concerning predictors that could be included in an integrated LT selection model and to evaluate the importance of biological aggressiveness in the evaluation process of these patients.

关键词： Hepatocellular carcinoma Liver Alphafetoprotein Transplantation Biomarkers Histopathology Recurrence Integrated prognostic tool

Management of hepatitis C infection before and after liver transplantation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2015年第15期21卷 4447-4456页

作者： Stefano Fagiuoli Roberto Ravasio Maria Grazia Lucà Anna Baldan Silvia Pecere Alessandro Vitale Luisa Pasulo Gastroenterology and Transplant Hepatology Papa Giovanni XXIII Hospital PHarm ES Milano Liver Transplantation and Hepatobiliary Surgical Unit Padua University Hospital

Chronic hepatitis C(CHC) is the most common indication for liver transplantation(LT). Aggressive treatment of hepatitis C virus(HCV) infection before cirrhosis development or decompensation may reduce LT need and risk of HCV recurrence post-LT. Factors associated with increased HCV risk or severity of recurrence include older age, immunosuppression, HCV genotype 1 and high viral load at LT. HCV recurrence post-LT leads to accelerated liver disease and cirrhosis development with reduced graft and patient survival. Currently, interferon(IFN)-based regimens can be used in dualagent regimens with ribavirin, in triple-agent antiviral strategies with direct-acting antivirals(e.g., protease inhibitors telaprevir or boceprevir), or before transplant in compensated patients to reduce HCV viral load to prevent or reduce the risk of post-LT recurrence and complications; they cannot be used in patients with decompensated cirrhosis. IFN-based regimens are used in less than half of HCV-infected patients waiting for LT due to extremely low efficacy and poor tolerability. However, antiviral therapy is indicated after LT in patients with histologically confirmed CHC despite tolerability issues. Improvements in side effect management have increased survival in patients achieving therapeutic targets. HCV treatment pre- and post-LT results in significant health care costs especially when lack of efficacy leads to disease worsening, although studies have shown sofosbuvir treatment before LT vs conventional post-LT dual antiviral is cost effective. The suboptimal efficacy and tolerability of IFN-based therapies, plus the significant economic burden, means the need for effective and well tolerated IFN-free antiHCV therapy for pre- and post-LT remains high.

关键词： Hepatitis C virus Orthotopic liver trans plantation Interferon-free treatment Decompensated cirrhosis Chronic hepatitis C

The Tenchini's collection:a forensic anthropometric legacy of 19th century Parma, Italy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Forensic Sciences Research 2019年第1期4卷 82-87页

作者： Laura Donato Roberto Toni alessandro Porro Marco vitale Fulvio Barbaro Rossana Cecchi Department of Medicine and Surgery Section of Forensic PathologyUniversity of ParmaParmaItaly Department of Medicine and Surgery Museum and Historical Library of Biomedicine(BIOMED)and Section of Human AnatomyUniversity of ParmaParmaItaly Department of Clinical and Community Sciences University of MilanMilanItaly Department of Medicine and Surgery Section of Human AnatomyUniversity of ParmaParmaItaly

A group of 19th century inmates dead in the prison of Parma are the protagonist of an incredible scientific collection. Lorenzo Tenchini started the creation of this collection and dedicated his work and his studies to its completeness. Anatomist and academic, Lorenzo Tenchini (1852–1906) dedicated his scientific studies to macroscopic anatomy, particularly about central nervous system and its correlation with psychic function. In 1881 he became ordinary professor in Normal Human Anatomy at the University of Parma dedicating himself to the study of the anatomical organization of the brain and psychic and social disturbs. During the study of the skulls and brains of psychotic patients and the deformations of skulls belonging to patients admitted in the Hospital of Brescia, he started a collaboration with alessandro Cugini (1829–1913), founder of the Institute of Legal Medicine at the University of Parma. Tenchini realized an anatomical collection, preserved today in the Museum of Biomedicine of the University of Parma. This collection represents the master-piece of his research carried out during his academic activity and still a unicum in the west-ern world, as there are no similar collection assembling such a multidisciplinary information. The peculiarity of this collection is due not only to the scientific interest of the anatomic samples and their full clinical documentation, but also to the methods employed in order to realize it. At the end of the 19th century, as a student of Cesare Lombroso (1835–1909), Tenchini based his work on the study of the face, the skull and brain of each dead inmate of Parma's prison or Colorno's mental hospital. These individuals as protagonists of Tenchini's collection, leave a legacy identifiable as scientific heritage. Their skulls and brains, the reproduction of their faces through ceroplastic and other anatomical samples treated with other techniques, are accompanied by an autoptic and psychiatric full documentation, allowing the collection to be complete with every aspect r

关键词： Anatomical collection death mask forensic sciences inmates multidisciplinary Parma

Protein Domains Involved in Assembly in the Endoplasmic Reticulum Promote Vacuolar Delivery when Fused to Secretory GFP, Indicating a Protein Quality Control Pathway for Degradation in the Plant Vacuole

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2008年第6期1卷 1067-1076页

作者： Ombretta Foresti Francesca De Marchis Maddalena de Virgilio Eva M. Klein Sergio Arcioni Michele Bellucci Alessandro Vitale Istituto di Biologia e Biotecnologia Agraria Consiglio Nazionale delle Ricerche via Bassini 15 20133 Milano Italy EU Istituto di Genetica Vegetale Consiglio Nazionale delle Ricerche via della Madonna Alta 130 06128 Perugia Italy EU

The correct folding and assembly of newly synthesized secretory proteins are monitored by the protein quality control system of the endoplasmic reticulum （ER）. Through interactions with chaperones such as the binding protein （BiP） and other folding helpers, quality control favors productive folding and sorts for degradation defective proteins. A major route for quality control degradation identified in yeast, plants, and animals is constituted by retrotranslocation from the ER to the cytosol and subsequent disposal by the ubiquitin/proteasome system, but alternative routes involving the vacuole have been identified in yeast. In this study, we have studied the destiny of sGFP418, a fusion between a secretory form of GFP and a domain of the vacuolar protein phaseolin that is involved in the correct assembly of phaseolin and in BiP recognition of unassembled subunits. We show that sGFP418, despite lacking the phaseolin vacuolar sorting signal, is delivered to the vacuole and fragmented, in a process that is inhibited by the secretory traffic inhibitor brefeldin A. Moreover, a fusion between GFP and a domain of the maize storage protein γ-zein involved in zein polymerization also undergoes post-translational fragmentation similar to that of sGFP418. These results show that defective secretory proteins with permanently exposed sequences normally involved in oligomerization can be delivered to the vacuole by secretory traffic. This strongly suggests the existence of a plant vacuolar sorting mechanism devoted to the disposal of defective secretory proteins.

关键词： Endoplasmic reticulum protein degradation protein traffic vacuole.

Grassland Height Assessment by Satellite Images

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Remote Sensing 2017年第1期6卷 40-53页

作者： alessandro Cimbelli Valerio vitale Environmental and Territorial Statistics Directorate National Institute of Statistics Rome Italy

Images collected by optical and radar satellite sensors represent a most viable solution for the extraction of biophysical parameters of the earth surface. The mid-resolution dataset acquired by Landsat and Sentinel satellites have recently become available free of charge for all users. At the same time, some software for image processing and GIS, like QGIS, R, and ImageJ, have reached a high level of maturity and a large community of users, thanks to their open source license. In this project, free satellite images and open source software have been used for the assessment of the grassland biomass. The overall goal is the enhancement of the statistics of grassland production and dried fodder for the animal breeding. Currently, the National Institute of Statistics collects this kind of dataset at the province level. The project consists in some “in situ” surveys in a specific site in central Italy and in the building of a regression model between the grassland heights and the corresponding radiometric values of the most relevant image bands.

关键词： Biomass Sentinel Landsat Grassland

Role of laparoscopic and robotic liver resection compared to open surgery in elderly hepatocellular carcinoma patients: a systematic review and meta-analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hepatoma Research 2020年第6期6卷 77-91页

作者： Alberto Brolese Marta Rigoni Alessandro Vitale Giovanni de Pretis Ivo Avancini CeciliaPravadelli Michela Frisinghelli Umberto Rozzanigo Giacomo Luppi Francesco Dionisi Stefano Marcucci Giovanni Viel Paolo Beltempo Cristina Prezzi Marco Frisini Marco Brolese Giandomenico Nollo Francesco A.Ciarleglio Department of General Surgery&HPB Unit(Chief:Alberto Brolese MD)Azienda Provinciale per i Servizi Sanitari(APSS)Santa Chiara HospitalTrento 38122Italy IRCS-Innovation and Clinical Health Research-Bruno Kessler Foundation(FBK) Trento 38123Italy Department of Surgical Oncological&Gastroenterological SciencesPadua University HospitalPadua 35128Italy Department of Hepatology&Gastroenterology APSSTrento 38122Italy Department of Oncology Azienda Provinciale per i Servizi Sanitari(APSS)Trento 38122Italy Department of Radiology Azienda Provinciale per i Servizi Sanitari(APSS)Trento 38122Italy Proton therapy Unit Azienda Provinciale per i Servizi Sanitari(APSS)Trento 38122Italy Department of Industrial Engineering BIOtech LabUniversity of TrentoTrento 38122Italy School of Medicine Padua UniversityPadua 35128Italy

Aim:This study aimed to compare mini-invasive liver resection(MILR)(laparoscopic/robotic approach)and open liver resection(OLR)for hepatocellular carcinoma(HCC)in elderly patients with regard to clinical and oncological outcomes through a comprehensive systematic review.Methods:The MEDLINE and Cochrane Library electronic databases were systematically searched from 2009 to December 2019 to identify relevant English written studies comparing MILR and OLR.The main endpoints were Child-Pugh score,serum total bilirubin level,comorbidity,presence/absence of cirrhosis,minor/major resection,challenge segment approach,operative time,estimated intraoperative blood loss,liver failure rate,morbidity according to the Clavien-Dindo classification,length of hospital stay(LOS),postoperative mortality,number of lesions,tumor size,readmission rate,recurrence rate and survival at 1,3 and 5 years after operation.Meta-analyses provided pooled relative risks and mean differences for these outcomes.Cut-off for"elderly age"was set at 65 years old.Results:Eight studies that evaluated 3051 patients who underwent liver resection for HCC,with 950 undergoing MILR and 2101 OLR,were included after the screening process.Blood loss,morbidity,and LOS showed statistical significance in favor of MILR.In particular,with respect to OLR,MILR decreased on average blood loss by 161.43 mL(95%CI:250.24-72.61),risk of morbidity by 42%(P<0.01),LOS by 4 days(95%CI:7-2),postoperative mortality risk by 47%(although not significantly,P=0.06).Major resections were significantly more common in the OLR group(P<0.0001).Recurrence,although not significant(P=0.06),must also be emphasized.The two surgical approaches were comparable with regard to the other outcomes investigated.Conclusion:Meta-analyses confirmed the advantages of MILR in terms of short perioperative outcomes,where it may promote the extension of liver resection to HCC patients with borderline liver function.MILR may be considered an important treatment option with significant benefits in the elderly a

关键词： Hepatocellular carcinoma HCC mini-invasive liver resection laparoscopic liver surgery robotic liver surgery open liver surgery meta-analysis