检索结果-南通市图书馆

Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells

World Journal of Stem Cells 2020年第12期12卷 1640-1651页

作者： Cintia DS Horinouchi maría Julia Barisón Anny W Robert Crisciele Kuligovski alessandra m aguiar BrunoDallagiovanna Stem Cells Basic Biology Laboratory Instituto Carlos ChagasCuritiba 81350010ParanáBrazil

BACKGROUND Human adipose-derived stromal/stem cells(hASCs)are one of the most useful types of mesenchymal stromal/stem cells,which are adult multipotent cells with great therapeutic potential for the treatment of several ***,for successful clinical application,it is critical that high-quality cells can be *** factors seem to be able to influence cell quality and performance,especially factors related to donors’intrinsic characteristics,such as ***,there is no consensus regarding this characteristic,and there is conflicting information in the *** To investigate the growth kinetics and differentiation potential of adipose-derived stem cells isolated from the lipoaspirates of elderly and young *** hASCs were harvested from liposuctioned adipose tissue obtained from female donors(aged 20-70 years).Cells were distributed into two groups according to age range:old hASCs(oASCs,≥55 years,n=9)and young hASCs(yASCs,≤35 years,n=9).For each group,immunophenotypic characterization was performed by flow *** doubling time was assessed over seven *** adipogenic potential evaluation,lipid deposits were assessed after 7 d,14 d and 21 d of adipogenic *** potential was verified by analyzing cell mineralization after 14 d,21 d and 28 d of osteogenic *** expression of PPARγ2,CEBPA and Runx2 were detected by quantitative reverse transcription polymerase chain *** hASCs were successfully obtained,cultured,and grouped according to their age:yASCs(26.33±4.66 years old)and oASCs(64.78±4.58 years old).After maintenance of the cells in culture,there were no differences in morphology between cells from the young and old ***,both groups showed classical immunophenotypic characteristics of mesenchymal stem/stromal *** average doubling time indicated that yASCs(4.09±0.94 d)did not significantly differ from oASCs(4.19±1.29 d).Concerning differentiation pot

关键词： Adipose-derived stem cells Stem cells Adipogenesis Osteogenesis Cell differentiation Donor age

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Terpenes extracted from marine sponges with antioxidant activity:a systematic review

引用

Natural Products and Bioprospecting 2023年第1期13卷 516-527页

作者： Cintia Cristina Santi martignago Beatriz Soares-Silva Julia Risso Parisi Lais Caroline Souza e Silva Renata Neves Granito alessandra mussi Ribeiro Ana Cláudia muniz Renno Lorena Ramos Freitas de Sousa Anna Caroline Campos aguiar Departamento de Biociências Universidade Federal de São Paulo(UNIFESP)Rua Silva Jardim 136Edifício CentralSantosSP 11015-020Brazil Institute of Chemistry Federal University of Catalão(UFCAT)Av.Dr.Lamartine Pinto de Avelar1120 Vila ChaudCatalãoGO 75704-020Brazil

marine biodiversity has emerged as a very promising resource of bioactive compounds and secondary metabolites from different sea *** sponge’s secondary metabolites demonstrated various bioactivities and potential pharmacological *** systematic review of the literature focuses on the advances achieved in the antioxidant potential of marine sponges in *** review was performed in accordance with PRISmA *** main inclusion criterion for analysis was articles with identification of compounds from terpene classes that demonstrate antioxidant activity in *** in three different databases,two hundred articles were *** screening abstracts,titles and evaluating for eligibility of manuscripts 14 articles were *** most performed analyzes to detect antioxidant activity were scavenging activity 2,2-diphenyl-1-picrylhydrazyl(DPPH)and measurement of intracellular reactive oxygen species(ROS).It was possible to identify 17 compounds of the terpene class with pronounced antioxidant activity in *** evidence of the studies included in this review was accessed by the GRADE *** play an important ecological role,moreover these molecules have a pharmaceutical and industrial application.

关键词： Antioxidant marine drugs Sponge Free radical

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Alkaline phosphatase-positive cells isolated from human hearts have mesenchymal stem cell characteristics

引用

Stem Cell Discovery 2011年第3期1卷 71-80页

作者： alessandra melo de aguiar Crisciele Kuligovski marise Teresinha Brenner Affonso da Costa marco Augusto Stimamiglio Carmen Lúcia Kuniyoshi Rebelatto Alexandra Cristina Senegaglia Paulo Roberto Slud Brofman Bruno Dallagiovanna Samuel Goldenberg Alejandro Correa 不详

Tissue-specific resident cells have been identified as a promising population of progenitor cells for cell-based therapies. We describe here the isolation from adult human hearts of tissue nonspecific alkaline phosphatase-positive cells (ALPL+ cells) with mesenchymal stem cell (mSC) characteristics. Samples from 24 adult cadaveric donors were obtained from a valve bank. mean total ischemia time was 21.5 ± 9.1 hours. The success rate for the isolation of human heart-derived cells by the explant culture technique was 70% for the right auricle (14 of 20 trials) and 33% for the right ventricle (7 of 21 trials). The total auricle-derived cell population (TAD) was used for the purification of ALPL+ cells. TAD and ALPL+ cells expressed markers for mSC and pericytes. TAD cells and ALPL+ cells differentiated into adipocytes, osteoblasts and chondroblasts, and ALPL+ cells expressed markers of these three lineages more strongly than TAD cells, as shown by RT-PCR. This population therefore has a greater potential for differentiation into mesechymal lineages than TAD cells. Both cell populations express some markers of cardiac progenitors, such as GATA4, CD117 and VEGF. ALPL+ cells expressed troponin T and ABCG2, which are also markers of the cardiac lineage. Heart samples from tissue banks could be considered as sources of mSC with putative commitment towards cardiac lineages, even after prolonged ischemia times.

关键词： Alkaline Phosphatase mesenchymal Stem Cell Pericyte Heart Cell Differentiation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：