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Over-expression of Ultrabithorax alters embryonic body plan and wing patterns in the butterfly Bicyclus anynana

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Over-expression of Ultrabithorax alters embryonic body plan ...

Potential of contemporary earth-sheltered buildings to achieve Plus Energy status in various European climates during the heating season

第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会

作者： Xiaoling Tong Steven Hrycaj Ondrej Podlaha aleksandar popadic Antónia Monteiro Department of Ecology and Evolutionary Biology Yale University State Key Laboratory of Silkworm Genome Biology Southwest University Department of Biological Sciences Wayne State University Department of Biological Sciences National University of Singapore

In insects, forewings and hindwings usually have different shapes, sizes, and color patterns. A variety of RNAi experiments across insect species have shown that the hox gene Ultrabithorax（Ubx） is necessary to promote hindwing identity. However, it remains unclear whether Ubx is sufficient to confer hindwing fate to forewings across insects. Here, we address this question by over-expressing Ubx in the butterfly Bicyclus anynana using a heat-shock promoter. Ubx whole-body over-expression during embryonic and larvae development led to body plan changes in larvae but to mere quantitative changes to adult morphology, respectively. Embryonic heat-shocks led to fused segments, loss of thoracic and abdominal limbs, and transformation of head limbs to larger appendages. Larval heat-shocks led to reduced eyespot size in the expected homeotic direction, but neither additional eyespots nor wing shape changes were observed in forewings as expected of a homeotic transformation. Interestingly, Ubx was found to be expressed in a novel, non-characteristic domain – in the hindwing eyespot centers. Furthermore, ectopic expression of Ubx on the pupal wing activated the eyespot-associated genes spalt and Distal-less, known to be directly repressed by Ubx in the fly’s haltere and leg primordia, respectively, and led to the differentiation of black wing scales. These results suggest that Ubx has been co-opted into a novel eyespot gene regulatory network, and that it is capable of activating black pigmentation in butterflies.

关键词： Ultrabithorax Over-expression Homeotic transformation Wing Eyespot Bicyclus anynana

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Building Simulation 2024年第1期17卷 41-52页

作者： aleksandar Nesovic Nebojsa Jurisevic Robert Kowalik lvana Terzic Faculty of Engineering University of KragujevacSestre Janjic 634000 KragujevacSerbia Faculty of Environmental Geomatic and Energy EngineeringKielce University of TechnologyTysigclecia Panstwa Polskiego725-314 KielcePoland

This study investigates the potential of contemporary Earth-Sheltered Buildings to contribute to sustainable societies by achieving Plus Energy Building status during the heating season.The buildings analyzed are intended to house a four-member family(102.5 m^(2)).The house is equipped with a ground source heat pump,geothermal vertical probes,floor heating panels,an energy recovery ventilator,and building-integrated photovoltaic panels installed on the exposed building wall.The numerical analysis(using EnergyPlus software)was carried out for nine European sites covering five different climatic zones(namely:southern,Atlantic,continental,alpine,and northern climate)and three latitude belts spanning Europe from south to north.Buildings above 65°N(northern and alpine climate)could not achieve Plus Energy status with 20% efficient BIPVs.With 15% efficient BIPVs,the building above 59°N(northern climate)was incapable of achieving this as well.Regarding the monthly time step,the buildings in the southern and Atlantic climates with 15% efficient BiPVs were able to produce an energy surplus every month,as was the building in theAlps with 20% efficientBIPVs.

关键词： civil engineering earth-sheltered buildings renewable energy sustainable societies Plus Energy Buildings Energy Plus software

Non-thermal atmospheric-pressure positive pulsating corona discharge in degradation of textile dye Reactive Blue 19 enhanced by Bi_(2)O_(3) catalyst

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Plasma Science and Technology 2024年第2期26卷 104-113页

作者： Milica PETROVIC Dragan RADIVOJEVIC Sasa RANCEV Nena VELINOV Milos KOSTIC Danijela BOJIC aleksandar BOJIC Department of Chemistry Faculty of Sciences and MathematicsUniversity of NisNis 18000Serbia Department of Physics Faculty of Sciences and MathematicsUniversity of NisNis 18000Serbia

In this work,monoclinic Bi_(2)O_(3) was applied for the first time,to the best of our knowledge,as a catalyst in the process of dye degradation by a non-thermal atmospheric-pressure positive pulsating corona discharge.The research focused on the interaction of the plasma-generated species and the catalyst,as well as the role of the catalyst in the degradation process.Plasma decomposition of the anthraquinone reactive dye Reactive Blue 19(RB 19) was performed in a selfmade reactor system.Bi_(2)O_(3) was prepared by electrodeposition followed by thermal treatment,and characterized by x-ray diffraction,scanning electron microscopy and energy-dispersive xray techniques.It was observed that the catalyst promoted decomposition of plasma-generated H_(2)O_(2) into ·OH radicals,the principal dye-degrading reagent,which further attacked the dye molecules.The catalyst improved the decolorization rate by 2.5 times,the energy yield by 93.4%and total organic carbon removal by 7.1%.Excitation of the catalyst mostly occurred through strikes by plasma-generated reactive ions and radical species from the air,accelerated by the electric field,as well as by fast electrons with an energy of up to 15 eV generated by the streamers reaching the liquid surface.These strikes transferred the energy to the catalyst and created the electrons and holes,which further reacted with H_(2)O_(2) and water,producing ·OH radicals.This was indentified as the primary role of the catalyst in this process.Decolorization reactions followed pseudo first-order kinetics.Production of H_(2)O_(2) and the dye degradation rate increased with increase in the input voltage.The optimal catalyst dose was 500 mg·dm^(-3).The decolorization rate was a little lower in river water compared with that in deionized water due to the side reactions of ·OH radicals with organic matter and inorganic ions dissolved in the river water.

关键词： corona RB 19 Bi_(2)O_(3) catalyst degradation

In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi

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Microsystems & Nanoengineering 2024年第2期10卷 251-267页

作者： Tanja Pajić Katarina Stevanović Nataša V.Todorović aleksandar J.Krmpot MiroslavŽivić Svetlana Savić-Šević Steva M.Lević Marina Stanić Dejan Pantelić Brana Jelenković Mihailo D.Rabasović Institute of Physiology and Biochemistry“Ivan Djaja” Faculty of BiologyUniversity of BelgradeStudentski trg 1611158BelgradeSerbia Institute for Biological Research“Siniša Stanković” University of BelgradeNational Institute of the Republic of SerbiaBulevar Despota Stefana 14211000BelgradeSerbia Institute of Physics Belgrade University of BelgradeNational Institute of the Republic of SerbiaPregrevica 11811080BelgradeSerbia University of Belgrade Faculty of AgricultureNemanjina Street 611080BelgradeSerbia Institute for Multidisciplinary Research University of BelgradeKneza Višeslava 111030BelgradeSerbia

Studying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing pathogens.However,their plasma membrane has been inaccessible for a micron-sized patch-clamp pipette for pA current recordings due to the rigid chitinous cell wall.Here,we report the first femtosecond IR laser nanosurgery of the cell wall of the filamentous fungi,which enabled patch-clamp measurements on protoplasts released from hyphae.A reproducible and highly precise(diffraction-limited,submicron resolution)method for obtaining viable released protoplasts was developed.Protoplast release from the nanosurgery-generated incisions in the cell wall was achieved from different regions of the hyphae.The plasma membrane of the obtained protoplasts formed tight and high-resistance(GΩ)contacts with the recording pipette.The entire nanosurgical procedure followed by the patch-clamp technique could be completed in less than 1 hour.Compared to previous studies using heterologously expressed channels,this technique provides the opportunity to identify new ionic currents and to study the properties of the ion channels in the protoplasts of filamentous fungi in their native environment.

关键词： patch recording laser

转录因子HNF1A、HNF4A和FOXA2调节肝细胞蛋白质N-糖基化

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Engineering 2024年第1期32卷 57-68页

作者： Vedrana Vicic Bockor Nika Foglar Goran Josipovic Marija Klasic Ana Vujic Branimir Plavsa Toma Keser Samira Smajlovic aleksandar Vojta Vlatka Zoldos Laboratory for Epigenetics Department of BiologyFaculty of ScienceUniversity of ZagrebZagreb 10000Croatia Genos Glycoscience Research Laboratory Zagreb 10000Croatia Faculty of Pharmacy and Biochemistry University of ZagrebZagreb 10000Croatia

Hepatocyte nuclear factor 1 alpha(HNF1A),hepatocyte nuclear factor 4 alpha(HNF4A),and forkhead box protein A2(FOXA2)are key transcription factors that regulate a complex gene network in the liver,cre-ating a regulatory transcriptional loop.The Encode and ChIP-Atlas databases identify the recognition sites of these transcription factors in many glycosyltransferase genes.Our in silico analysis of HNF1A,HNF4A.and FOXA2 binding to the ten candidate glyco-genes studied in this work confirms a significant enrich-ment of these transcription factors specifically in the liver.Our previous studies identified HNF1A as a master regulator of fucosylation,glycan branching,and galactosylation of plasma glycoproteins.Here,we aimed to functionally validate the role of the three transcription factors on downstream glyco-gene transcriptional expression and the possible effect on glycan phenotype.We used the state-of-the-art clus-tered regularly interspaced short palindromic repeats/dead Cas9(CRISPR/dCas9)molecular tool for the downregulation of the HNF1A,HNF4A,and FOXA2 genes in HepG2 cells-a human liver cancer cell line.The results show that the downregulation of all three genes individually and in pairs affects the transcrip-tional activity of many glyco-genes,although downregulation of glyco-genes was not always followed by an unambiguous change in the corresponding glycan structures.The effect is better seen as an overall change in the total HepG2 N-glycome,primarily due to the extension of biantennary glycans.We propose an alternative way to evaluate the N-glycome composition via estimating the overall complexity of the glycome by quantifying the number of monomers in each glycan structure.We also propose a model showing feedback loops with the mutual activation of HNF1A-FOXA2 and HNF4A-FOXA2 affecting glyco-genes and protein glycosylation in HepG2 cells.

关键词： Clustered regularly interspaced short palindromic repeats/dead Cas9(CRISPR/dCas9) Epigenetics Hepatocyte nuclear factor 1 alpha(HNF1A) Hepatocyte nuclear factor 4 alpha(HNF4A) Forkhead box protein A2(FOXA2) N-glycosylation HepG2 cells

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时间隔离——一种新的控制交尾模式

中国蜂业 2024年第5期75卷 22-23页

作者： aleksandar Uzunov Marin Kovacic 陈超单金琼中国农业科学院蜜蜂研究所资源昆虫高效养殖与利用全国重点实验室北京100193 北马其顿圣基里尔·麦托迪大学农业科学与食品学院斯科普里1000 克罗地亚奥西耶克大学农业生物技术科学学院奥西耶克31000

控制交尾是蜜蜂育种项目中遗传改良和保护的关键因素。在上一期文章中,我们讨论了控制交尾的四种可选的模式及其相关性,即地理隔离、生物隔离、人工授精和时间隔离。实际上,最后一种模式在近几十年刚刚发展起来,可以作为传统模式的一种... 详细信息

控制交尾是蜜蜂育种项目中遗传改良和保护的关键因素。在上一期文章中,我们讨论了控制交尾的四种可选的模式及其相关性,即地理隔离、生物隔离、人工授精和时间隔离。实际上,最后一种模式在近几十年刚刚发展起来,可以作为传统模式的一种替代模式。在本文中,我们更加详细的介绍时间隔离的概念及其变体,这种模式吸引了蜜蜂育种人员和育王人员的高度关注。

关键词：地理隔离时间隔离育王遗传改良替代模式蜜蜂育种交尾人工授精

Evaluation of stability and functionality of BaCe_(1−x)In_(x)O_(3−δ) electrolyte in a wider range of indium concentration

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Journal of Advanced Ceramics 2022年第3期11卷 443-453页

作者： aleksandar MALESEVIC aleksandar RADOJKOVIC Milan ZUNIC Aleksandra DAPCEVIC Sanja PERAC Zorica BRANKOVIC Goran BRANKOVIC Center of Excellence for Green Technologies Institute for Multidisciplinary ResearchUniversity of BelgradeKneza Viseslava 711030 BelgradeSerbia Faculty of Technology and Metallurgy University of BelgradeKarnegijeva 411120 BelgradeSerbia

The properties of BaCe_(1−x)In_(x)O_(3−δ) (x = 0.05, 0.10, 0.15, 0.20, _(0.25), 0.30, 0.35, and 0.40) as proton conducting electrolyte are examined. The dense electrolyte is formed after sintering at 1300 ℃ for 5 h in air. The samples with In content ≥ 25 mol% contain In_(2)O_(3) as a secondary phase. The highest total conductivity is around 5×10^(−3) S/cm for BaCe_(0.75)In_(0.25)O_(3−δ) in the wet hydrogen atmosphere at 700 ℃. After exposure to pure CO_(2) atmosphere at 700 ℃ for 5 h, the concentrations of at least 15 mol% In can completely suppress degradation of the electrolyte. The power density of Ni-BaCe_(0.75)In_(0.25)O_(3−δ)/BaCe_(0.75)In_(0.25)O_(3−δ)/LSCF-BaCe_(0.75)In_(0.25)O_(3−δ) fuel cell tested in wet hydrogen atmosphere reaches 264 mW/cm2 at 700 ℃. This result is an indication of stability and functionality of this electrolyte and its versatility in respect to type of fuel and performing environment.

关键词： ionic conductivity perovskite fuel cell BaCeO_(3)

Computational Methods for Single-cell Multi-omics Integration and Alignment

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Genomics, Proteomics & Bioinformatics 2022年第5期20卷 836-849页

作者： Stefan Stanojevic Yijun Li aleksandar Ristivojevic Lana X.Garmire Department of Computational Medicine and Bioinformatics University of MichiganAnn ArborMI 48109USA Department of Biostatistics University of MichiganAnn ArborMI 48109USA Amherst College AmherstMA 01002USA

Recently developed technologies to generate single-cell genomic data have made a revolutionary impact in the field of biology.Multi-omics assays offer even greater opportunities to understand cellular states and biological processes.The problem of integrating different omics data with very different dimensionality and statistical properties remains,however,quite challenging.A growing body of computational tools is being developed for this task,leveraging ideas ranging from machine translation to the theory of networks,and represents another frontier on the interface of biology and data science.Our goal in this review is to provide a comprehensive,up-to-date survey of computational techniques for the integration of single-cell multi-omics data,while making the concepts behind each algorithm approachable to a non-expert audience.

关键词： Single-cell Multi-omics Machine learning Unsupervised learning Integration

Hyperledger fabric platform for healthcare trust relations—Proof-of-Concept

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Blockchain(Research and Applications) 2023年第4期4卷 63-74页

作者： aleksandar Nedakovic Anton Hasselgren Katina Kralevska Danilo Gligoroski Department of Information Security and Communication Technology Norwegian University of Science and Technology(NTNU)7034 TrondheimNorway Department of Neuromedicine and Movement Science Norwegian University of Science and Technology(NTNU)7034 TrondheimNorway

In recent years,blockchain technologies have expanded from the finance field to other areas that rely on trustbased solutions.The healthcare industry represents one such area,as digital transformation disrupts relationships between patients,healthcare professionals,and healthcare institutes.Patients and healthcare institutes lack a proficient tool to verify the credentials of medical professionals in a digital environment.Furthermore,healthcare professionals lack a tool where they are in control over their credentials.The first contribution of this paper is a proposal of a solution that leverages the private permissioned Hyperledger Fabric blockchain and smart contracts to provide a source of transparent trust for relationships within the healthcare industry.Second,we pave the ground for GDPR compliance by storing only the hash values on the blockchain.Third,we solve the problem of patient authentication by utilizing cryptographic techniques.Finally,we prove the usability of the proposed solution by implementing a user interface and creating a live deployment.

关键词： Blockchain Hyperledger fabric Health systems Distributed ledger