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In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi

Microsystems & Nanoengineering 2024年第2期10卷 251-267页

作者： Tanja Pajić Katarina Stevanović Nataša V.Todorović aleksandar j.krmpot Miroslav Živić Svetlana Savić-Šević Steva M.Lević Marina Stanić Dejan Pantelić Brana jelenković Mihailo D.Rabasović Institute of Physiology and Biochemistry“Ivan Djaja” Faculty of BiologyUniversity of BelgradeStudentski trg 1611158BelgradeSerbia Institute for Biological Research“Siniša Stanković” University of BelgradeNational Institute of the Republic of SerbiaBulevar Despota Stefana 14211000BelgradeSerbia Institute of Physics Belgrade University of BelgradeNational Institute of the Republic of SerbiaPregrevica 11811080BelgradeSerbia University of Belgrade Faculty of AgricultureNemanjina Street 611080BelgradeSerbia Institute for Multidisciplinary Research University of BelgradeKneza Višeslava 111030BelgradeSerbia

Studying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing ***,their plasma membrane has been inaccessible for a micron-sized patch-clamp pipette for pA current recordings due to the rigid chitinous cell ***,we report the first femtosecond IR laser nanosurgery of the cell wall of the filamentous fungi,which enabled patch-clamp measurements on protoplasts released from hyphae.A reproducible and highly precise(diffraction-limited,submicron resolution)method for obtaining viable released protoplasts was *** release from the nanosurgery-generated incisions in the cell wall was achieved from different regions of the *** plasma membrane of the obtained protoplasts formed tight and high-resistance(GΩ)contacts with the recording *** entire nanosurgical procedure followed by the patch-clamp technique could be completed in less than 1 *** to previous studies using heterologously expressed channels,this technique provides the opportunity to identify new ionic currents and to study the properties of the ion channels in the protoplasts of filamentous fungi in their native environment.

关键词： patch recording laser

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A resilience-based approach in managing the closure and abandonment of large mine tailing ponds

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International journal of Mining Science and Technology 2020年第5期30卷 737-746页

作者： Dragan Komljenovic Lazar Stojanovic Vladimir Malbasic aleksandar Lukic Hydro-Québec Research Institute(IREQ) VarennesQC J3X 1S1Canada Faculty of Mining University of Banja Luka79101 PrijedorBosnia and Herzegovina ArcelorMittal Prijedor d.o.o.Prijedor 79101 PrijedorBosnia and Herzegovina

Mining tailing ponds are large infrastructure objects whose life cycle spans over several *** are indispensable for certain types of mines where technological process produces and rejects *** also have potential to generate risks for human life,property and *** that reason,it is essential to adequately manage them throughout all the stages of their life *** phase of their closure and abandonment is less studied and *** paper proposes a holistic resilience-based approach for analyzing this phase of their life *** proposed methodology is validated through a case study at an actual surface iron ore mine in Bosnia and Herzegovina.

关键词： Mine tailing ponds Closure Abandonment Resilience Risks Uncertainties Complexity

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Reply to “Is physical performance (in mice) increased by Veillonella atypica or decreased by Lactobacillus bulgaricus?”

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journal of Sport and Health Science 2020年第3期9卷 201-202页

作者： aleksandar D.Kostic Section on Pathophysiology and Molecular Pharmacology Joslin Diabetes CenterBostonMA 02215USA Department of Microbiology Harvard Medical SchoolBostonMA 02215USA

Dear editor,In a recent longitudinal metagenomic study of the gut microbiome in elite athletes,1 we conducted multiple, repeated sampling from runners in the 2015 Boston Marathon as well as sedentary controls approximately 1 week prior to and 1 week after marathon day. We also analyzed an independent cohort of endurance athletes, including Olympic-caliber rowers。

关键词： elite editor Boston

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Response of Growth Dynamics of Two Spring Geophytes to Light Regime in a Lime-Beech Forest

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journal of Integrative Plant Biology 2006年第5期48卷 527-535页

作者： Zorica Popovic aleksandar Mijovic Branko Karadzic Miroslava Mijatovic Institute of Biological Research Department of Ecology Despota Stefana Boulevard 142 11060 Belgrade Serbia and Montenegro Environment Protection Agency Ministry for Science and Environmental Protection Republic of Serbia Serbia and Montenegro

Blomaee accumulation, leaf longevity and growth rate of two spring forest geophytes, Scllla blfolla L. and Arum maculatum L. were estimated separately for three size groups within each population of these species. Despite the differences in leaf longevity, both species showed a similar pattern of blomass accumulation In relation to their phenologles and reproductive demands. Eco-physlological acclimation to changing light environment was assumed through photosynthetic parameters and dynamics of leaf area Index In the predominant size group of each species. A light response curve was measured under natural light for each species through the continuum of Its phenology to quantify the photosynthetic photon flux density at light saturation, light-saturated photosynthetic rate, light compensation point, and dark respiration. Light-saturated assimilation per leaf area basis, dark respiration rate and light compensation points were significantly higher in S. blfolla relative to A. maculatum. However, the acclimation of photosynthesis that would respond to light changes in environment was not found in S. bifolla. In contrast, In A. maculatum a marked shift In the light dependence of photosynthesis through the season was noticed, which resulted In a strong photosynthetic acclimation to the low-light conditions. Accompanied by significant leaf area Index, this efficient low-light photosynthesis enabled greater leaf longevity, and consequently longer accumulative period to A. maculatum. From the different parameters that we determined （both photosynthetic acclimation and growth strategy） it would appear that these species belong to two distinct subgroups： S. blfolla to the early and A. maculatum to the late vernals.

关键词： biomass accumulation deciduous forests early and late vernals leaf area index (LAI) light response of photosynthesis spring geophytes.

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Diesel and welding aerosols in an underground mine

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International journal of Mining Science and Technology 2020年第4期30卷 449-454页

作者： aleksandar D.Bugarski Teresa L.Barone jon A.Hummer National Institute for Occupational Safety and Health Pittsburgh Mining Research DivisionPittsburghPA 15236USA

Researchers from the National Institute for Occupational Safety and Health(NIOSH)conducted a study in an isolated zone of an underground mine to characterize aerosols generated by:(1)a diesel-powered personnel carrier vehicle operated over a simulated light-duty cycle and(2)the simulated repair of existing equipment using manual metal arc welding(MMAW).Both the diesel-powered vehicle and MMAW process contributed to concentrations of nano and ultrafine aerosols in the mine *** welding process also contributed to aerosols with electrical mobility and aerodynamic mobility count median diameters of approximately 140 and 480 nm,*** welding particles collected on the filters contained carbon,iron,manganese,calcium,and aluminum.

关键词： Diesel aerosols Welding aerosols Underground mining Size distribution

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Hyperledger fabric platform for healthcare trust relations—Proof-of-Concept

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Blockchain(Research and Applications) 2023年第4期4卷 63-74页

作者： aleksandar Nedakovic Anton Hasselgren Katina Kralevska Danilo Gligoroski Department of Information Security and Communication Technology Norwegian University of Science and Technology(NTNU)7034 TrondheimNorway Department of Neuromedicine and Movement Science Norwegian University of Science and Technology(NTNU)7034 TrondheimNorway

In recent years,blockchain technologies have expanded from the finance field to other areas that rely on trustbased *** healthcare industry represents one such area,as digital transformation disrupts relationships between patients,healthcare professionals,and healthcare *** and healthcare institutes lack a proficient tool to verify the credentials of medical professionals in a digital ***,healthcare professionals lack a tool where they are in control over their *** first contribution of this paper is a proposal of a solution that leverages the private permissioned Hyperledger Fabric blockchain and smart contracts to provide a source of transparent trust for relationships within the healthcare ***,we pave the ground for GDPR compliance by storing only the hash values on the ***,we solve the problem of patient authentication by utilizing cryptographic ***,we prove the usability of the proposed solution by implementing a user interface and creating a live deployment.

关键词： Blockchain Hyperledger fabric Health systems Distributed ledger

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Computational Methods for Single-cell Multi-omics Integration and Alignment

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Genomics, Proteomics & Bioinformatics 2022年第5期20卷 836-849页

作者： Stefan Stanojevic Yijun Li aleksandar Ristivojevic Lana X.Garmire Department of Computational Medicine and Bioinformatics University of MichiganAnn ArborMI 48109USA Department of Biostatistics University of MichiganAnn ArborMI 48109USA Amherst College AmherstMA 01002USA

Recently developed technologies to generate single-cell genomic data have made a revolutionary impact in the field of ***-omics assays offer even greater opportunities to understand cellular states and biological *** problem of integrating different omics data with very different dimensionality and statistical properties remains,however,quite challenging.A growing body of computational tools is being developed for this task,leveraging ideas ranging from machine translation to the theory of networks,and represents another frontier on the interface of biology and data *** goal in this review is to provide a comprehensive,up-to-date survey of computational techniques for the integration of single-cell multi-omics data,while making the concepts behind each algorithm approachable to a non-expert audience.

关键词： Single-cell Multi-omics Machine learning Unsupervised learning Integration

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Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation

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Chinese journal of Chemical Engineering 2020年第4期28卷 1136-1144页

作者： jelena Bebic Katarina Banjanac Marija Corović Ana Milivojevic Milica Simovic aleksandar Marinkovic Dejan Bezbradica Directorate of Measures and Precious Metals Mike Alasa 1411000 BelgradeSerbia Innovation Centre of Faculty of Technology and Metallurgy University of BelgradeKarnegijeva 411000 BelgradeSerbia Department of Biochemical Engineering and Biotechnology Faculty of Technology and MetallurgyUniversity of BelgradeKarnegijeva 411000 BelgradeSerbia Department of Organic Chemistry Faculty of Technology and MetallurgyUniversity of BelgradeKarnegijeva 411000 BelgradeSerbia

This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oryzae(Novozym 51003?laccase)on amino modified fumed nano-silica(AFNS)and the possible use in ***,for the first time,factors affecting the immobilization of Novozym 51003?laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase *** highest specific activity(13.1 IU·mg-1 proteins)was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield,reaching68.3%after the equilibrium time,at optimum pH 5.0,was *** immobilization occurs by adsorption as monolayer enzyme binding in 40 min,following pseudo-first-order *** possible use of obtained immobilized preparation was investigated in degradation of pesticide *** 24 h,lindane concentration was reduced to 56.8%of initial concentration and after seven repeated reuses it retained 70%of the original activity.

关键词： Silica nanoparticles Laccase Enzyme immobilization Lindane Bioremediation

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潮流建模中的不确定性——边界潮流法的应用(英文)

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电力系统自动化 2005年第16期29卷 6-15页

作者： aleksandar Dimitrovski Kevin Tomsovic Washington StateUniversityPullman WA 99164美国

回顾了边界潮流法最新研究进展和应用现状。边界潮流法是一种通过赋予不确定节点功率以模糊/区间数来找到精确潮流解的方法。边界潮流法得到的结果可以纳入系统不充裕指标的形式中。这些指标可用于风险评估——决策过程中的基本部分。11... 详细信息

回顾了边界潮流法最新研究进展和应用现状。边界潮流法是一种通过赋予不确定节点功率以模糊/区间数来找到精确潮流解的方法。边界潮流法得到的结果可以纳入系统不充裕指标的形式中。这些指标可用于风险评估——决策过程中的基本部分。118节点测试系统的数字仿真结果表明了所提方法的适用性。

关键词：连续潮流模糊集合区间算法潮流分析电力系统规划风险评估不确定性

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Eruptive xanthomas associated with diabetes mellitus

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Chinese Medical journal 2009年第17期122卷 2074-2075页

作者： Ivana Binic aleksandar jankovic Clinic of Dermatology and Venereology Clinical Center Nis Bid.Zorana Djindjica 48 18000 Nis Serbia

Diabetes mellitus （DM） is a most common endocrine disorder and it is characterized by high serum glucose levels and by disturbance of lipid metabolism. As a result, the patients can develop long-term systemic complications. Numerous skin lesions are associated with either type 1 or type 2 diabetes mellitus, and they are specific chronic complications of the disease. Cutaneous xanthomas result from deposition of lipids in the histiocytes in the dermis or subcutaneous tissue. Eruptive xanthomas are a characteristic, but uncommon complication of diabetes mellitus associated with a more sustained hyperlipidemia affecting plasma triglycerides and cholesterol, and hyperglycemia with glycosuria.

关键词： eruptive xanthomas diabetes mellitus hypertriglyceridemia hypercholesterolemia

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