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Potential of contemporary earth-sheltered buildings to achieve Plus Energy status in various European climates during the heating season

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Building Simulation 2024年第1期17卷 41-52页

作者： aleksandar Nesovic Nebojsa Jurisevic Robert Kowalik lvana Terzic Faculty of Engineering University of KragujevacSestre Janjic 634000 KragujevacSerbia Faculty of Environmental Geomatic and Energy EngineeringKielce University of TechnologyTysigclecia Panstwa Polskiego725-314 KielcePoland

This study investigates the potential of contemporary Earth-Sheltered Buildings to contribute to sustainable societies by achieving Plus Energy Building status during the heating *** buildings analyzed are intended to house a four-member family(102.5 m^(2)).The house is equipped with a ground source heat pump,geothermal vertical probes,floor heating panels,an energy recovery ventilator,and building-integrated photovoltaic panels installed on the exposed building *** numerical analysis(using EnergyPlus software)was carried out for nine European sites covering five different climatic zones(namely:southern,Atlantic,continental,alpine,and northern climate)and three latitude belts spanning Europe from south to *** above 65°N(northern and alpine climate)could not achieve Plus Energy status with 20% efficient *** 15% efficient BIPVs,the building above 59°N(northern climate)was incapable of achieving this as *** the monthly time step,the buildings in the southern and Atlantic climates with 15% efficient BiPVs were able to produce an energy surplus every month,as was the building in theAlps with 20% efficientBIPVs.

关键词： civil engineering earth-sheltered buildings renewable energy sustainable societies Plus Energy Buildings Energy Plus software

Non-thermal atmospheric-pressure positive pulsating corona discharge in degradation of textile dye Reactive Blue 19 enhanced by Bi_(2)O_(3) catalyst

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Plasma Science and Technology 2024年第2期26卷 104-113页

作者： Milica PETROVIC Dragan RADIVOJEVIC Sasa RANCEV Nena VELINOV Milos KOSTIC Danijela BOJIC aleksandar BOJIC Department of Chemistry Faculty of Sciences and MathematicsUniversity of NisNis 18000Serbia Department of Physics Faculty of Sciences and MathematicsUniversity of NisNis 18000Serbia

In this work,monoclinic Bi_(2)O_(3) was applied for the first time,to the best of our knowledge,as a catalyst in the process of dye degradation by a non-thermal atmospheric-pressure positive pulsating corona *** research focused on the interaction of the plasma-generated species and the catalyst,as well as the role of the catalyst in the degradation *** decomposition of the anthraquinone reactive dye Reactive Blue 19(RB 19) was performed in a selfmade reactor ***_(2)O_(3) was prepared by electrodeposition followed by thermal treatment,and characterized by x-ray diffraction,scanning electron microscopy and energy-dispersive xray *** was observed that the catalyst promoted decomposition of plasma-generated H_(2)O_(2) into ·OH radicals,the principal dye-degrading reagent,which further attacked the dye *** catalyst improved the decolorization rate by 2.5 times,the energy yield by 93.4%and total organic carbon removal by 7.1%.Excitation of the catalyst mostly occurred through strikes by plasma-generated reactive ions and radical species from the air,accelerated by the electric field,as well as by fast electrons with an energy of up to 15 eV generated by the streamers reaching the liquid *** strikes transferred the energy to the catalyst and created the electrons and holes,which further reacted with H_(2)O_(2) and water,producing ·OH *** was indentified as the primary role of the catalyst in this *** reactions followed pseudo first-order *** of H_(2)O_(2) and the dye degradation rate increased with increase in the input *** optimal catalyst dose was 500 mg·dm^(-3).The decolorization rate was a little lower in river water compared with that in deionized water due to the side reactions of ·OH radicals with organic matter and inorganic ions dissolved in the river water.

关键词： corona RB 19 Bi_(2)O_(3) catalyst degradation

In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi

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Microsystems & Nanoengineering 2024年第2期10卷 251-267页

作者： Tanja Pajić Katarina Stevanović Nataša V.Todorović aleksandar J.Krmpot Miroslav Živić Svetlana Savić-Šević Steva M.Lević Marina Stanić Dejan Pantelić Brana Jelenković Mihailo D.Rabasović Institute of Physiology and Biochemistry“Ivan Djaja” Faculty of BiologyUniversity of BelgradeStudentski trg 1611158BelgradeSerbia Institute for Biological Research“Siniša Stanković” University of BelgradeNational Institute of the Republic of SerbiaBulevar Despota Stefana 14211000BelgradeSerbia Institute of Physics Belgrade University of BelgradeNational Institute of the Republic of SerbiaPregrevica 11811080BelgradeSerbia University of Belgrade Faculty of AgricultureNemanjina Street 611080BelgradeSerbia Institute for Multidisciplinary Research University of BelgradeKneza Višeslava 111030BelgradeSerbia

Studying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing ***,their plasma membrane has been inaccessible for a micron-sized patch-clamp pipette for pA current recordings due to the rigid chitinous cell ***,we report the first femtosecond IR laser nanosurgery of the cell wall of the filamentous fungi,which enabled patch-clamp measurements on protoplasts released from hyphae.A reproducible and highly precise(diffraction-limited,submicron resolution)method for obtaining viable released protoplasts was *** release from the nanosurgery-generated incisions in the cell wall was achieved from different regions of the *** plasma membrane of the obtained protoplasts formed tight and high-resistance(GΩ)contacts with the recording *** entire nanosurgical procedure followed by the patch-clamp technique could be completed in less than 1 *** to previous studies using heterologously expressed channels,this technique provides the opportunity to identify new ionic currents and to study the properties of the ion channels in the protoplasts of filamentous fungi in their native environment.

关键词： patch recording laser

转录因子HNF1A、HNF4A和FOXA2调节肝细胞蛋白质N-糖基化

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Engineering 2024年第1期32卷 57-68页

作者： Vedrana Vicic Bockor Nika Foglar Goran Josipovic Marija Klasic Ana Vujic Branimir Plavsa Toma Keser Samira Smajlovic aleksandar Vojta Vlatka Zoldos Laboratory for Epigenetics Department of BiologyFaculty of ScienceUniversity of ZagrebZagreb 10000Croatia Genos Glycoscience Research Laboratory Zagreb 10000Croatia Faculty of Pharmacy and Biochemistry University of ZagrebZagreb 10000Croatia

Hepatocyte nuclear factor 1 alpha(HNF1A),hepatocyte nuclear factor 4 alpha(HNF4A),and forkhead box protein A2(FOXA2)are key transcription factors that regulate a complex gene network in the liver,cre-ating a regulatory transcriptional *** Encode and ChIP-Atlas databases identify the recognition sites of these transcription factors in many glycosyltransferase *** in silico analysis of HNF1A,*** FOXA2 binding to the ten candidate glyco-genes studied in this work confirms a significant enrich-ment of these transcription factors specifically in the *** previous studies identified HNF1A as a master regulator of fucosylation,glycan branching,and galactosylation of plasma ***,we aimed to functionally validate the role of the three transcription factors on downstream glyco-gene transcriptional expression and the possible effect on glycan *** used the state-of-the-art clus-tered regularly interspaced short palindromic repeats/dead Cas9(CRISPR/dCas9)molecular tool for the downregulation of the HNF1A,HNF4A,and FOXA2 genes in HepG2 cells-a human liver cancer cell *** results show that the downregulation of all three genes individually and in pairs affects the transcrip-tional activity of many glyco-genes,although downregulation of glyco-genes was not always followed by an unambiguous change in the corresponding glycan *** effect is better seen as an overall change in the total HepG2 N-glycome,primarily due to the extension of biantennary *** propose an alternative way to evaluate the N-glycome composition via estimating the overall complexity of the glycome by quantifying the number of monomers in each glycan *** also propose a model showing feedback loops with the mutual activation of HNF1A-FOXA2 and HNF4A-FOXA2 affecting glyco-genes and protein glycosylation in HepG2 cells.

关键词： Clustered regularly interspaced short palindromic repeats/dead Cas9(CRISPR/dCas9) Epigenetics Hepatocyte nuclear factor 1 alpha(HNF1A) Hepatocyte nuclear factor 4 alpha(HNF4A) Forkhead box protein A2(FOXA2) N-glycosylation HepG2 cells

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时间隔离——控制交尾的替代方案及新发展

中国蜂业 2024年第6期75卷 26-27页

作者： aleksandar Uzunov Marin Kovacic 段宇杰陈超中国农业科学院蜜蜂研究所资源昆虫高效养殖与利用全国重点实验室北京100193 北马其顿圣基里尔·麦托迪大学农业科学与食品学院斯科普里1000 克罗地亚奥西耶克大学农业生物技术科学学院奥西耶克31000

时间隔离的第二种方法被称为“迷宫”,这个装置可以将蜂王限制在交尾箱中或将其释放。该方法不需要黑暗和寒冷的环境,并且配备该装置的交尾箱可以始终放置在室外。这让蜂农的工作变得更简单,而且由于无需制冷设备,该装置的成本也会较低... 详细信息

时间隔离的第二种方法被称为“迷宫”,这个装置可以将蜂王限制在交尾箱中或将其释放。该方法不需要黑暗和寒冷的环境,并且配备该装置的交尾箱可以始终放置在室外。这让蜂农的工作变得更简单,而且由于无需制冷设备,该装置的成本也会较低。然而,交尾箱需要安装迷宫进行改装。下面简要介绍一下迷宫这种装置以及操作步骤。

关键词：交尾箱时间隔离制冷设备替代方案迷宫操作步骤蜂王蜂农

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时间隔离——一种新的控制交尾模式

中国蜂业 2024年第5期75卷 22-23页

作者： aleksandar Uzunov Marin Kovacic 陈超单金琼中国农业科学院蜜蜂研究所资源昆虫高效养殖与利用全国重点实验室北京100193 北马其顿圣基里尔·麦托迪大学农业科学与食品学院斯科普里1000 克罗地亚奥西耶克大学农业生物技术科学学院奥西耶克31000

控制交尾是蜜蜂育种项目中遗传改良和保护的关键因素。在上一期文章中,我们讨论了控制交尾的四种可选的模式及其相关性,即地理隔离、生物隔离、人工授精和时间隔离。实际上,最后一种模式在近几十年刚刚发展起来,可以作为传统模式的一种... 详细信息

控制交尾是蜜蜂育种项目中遗传改良和保护的关键因素。在上一期文章中,我们讨论了控制交尾的四种可选的模式及其相关性,即地理隔离、生物隔离、人工授精和时间隔离。实际上,最后一种模式在近几十年刚刚发展起来,可以作为传统模式的一种替代模式。在本文中,我们更加详细的介绍时间隔离的概念及其变体,这种模式吸引了蜜蜂育种人员和育王人员的高度关注。

关键词：地理隔离时间隔离育王遗传改良替代模式蜜蜂育种交尾人工授精

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以抗螨选育为重点的德国蜜蜂育种

中国蜂业 2024年第7期75卷 28-29页

作者： Ralph Büchler aleksandar Uzunov 程瑞益陈超单金琼德国黑森州蜜蜂研究所基希海因35274 北马其顿圣基里尔·麦托迪大学农业科学与食品学院斯科普里1000 中国农业科学院蜜蜂研究所资源昆虫高效养殖与利用全国重点实验室北京100193

一、德国蜜蜂育种的发展1870年前后,不同欧洲国家间的蜂王贸易蓬勃发展,这与德国采用活框饲养密切相关。在几十年的时间里,这个区域拥有了五花八门的蜂种,导致了本土黑蜂(欧洲黑蜂,Apis mellifera mellifera)的严重杂化。蜂种的自由混... 详细信息

一、德国蜜蜂育种的发展1870年前后,不同欧洲国家间的蜂王贸易蓬勃发展,这与德国采用活框饲养密切相关。在几十年的时间里,这个区域拥有了五花八门的蜂种,导致了本土黑蜂(欧洲黑蜂,Apis mellifera mellifera)的严重杂化。蜂种的自由混合导致蜂群攻击性和分蜂性增加、产蜜量变差。

关键词：蜂种分蜂蜜蜂育种欧洲黑蜂产蜜量活框饲养抗螨蜂王

Development of Dendritic Cell System

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Cellular & Molecular Immunology 2004年第2期1卷 112-118页

作者： aleksandar Dakic The Walter and Eliza Hall Institute of Medical Research IGRoyal ParadeParkvilleVictoria 3050Australia.

The dendritic cell system contains conventional dendritic cells(DCs)and plasmacytoid pre-dendritic cells (pDCs).Both DCs and pDCs are bone marrow derived *** the common functions of DCs are antigen-processing and T-lymphocyte activation,they differ in surface markers,migratory patterns,and cytokine *** differences can determine the fate of the T cells they *** subsets of mature DCs have been described in both mouse and human and the developmental processes of these specialized DC subsets have been studied *** original concept that all DCs were of myeloid origin was questioned by several recent studies,which demonstrated that in addition to the DCs derived from myeloid precursors, some DCs could also be efficiently generated from lymphoid-restricted ***,it has been shown recently that both conventional DCs and pDCs can be generated by the Flt3 expressing hemopoietic pro- genitors regardless of their myeloid-or *** findings suggest an early developmental flexibility of precursors for DCs and *** review summarizes some recent observations on the deve- lopment of DC system in both human and *** & Molecular Immunology.2004;1(2):112-118.

关键词： dendritic cell development hemopoietic precursor plasmacytoid dendritic cell Flt3

Integer Codes Correcting Single Errors and Random Asymmetric Errors within a Byte

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Journal of Systems Science & Complexity 2020年第6期33卷 2103-2113页

作者： RADONJIC aleksandar VUJICIC Vladimir Institute of Technical Sciences of the Serbian Academy of Sciences and Arts BelgradeSerbia

In optical networks without optical amplifiers(ONWOAs) photons may fade or fail to be detected, but new photons cannot be generated. Hence, under normal conditions, only 1 → 0 errors can occur. However, in some situations, the photodetector may generate a false 0 → 1 error. This mostly occurs in cases when the dark current is higher than specified. With this in mind, in this paper,the authors present a class of codes suitable for use in ONWOAs using self-synchronous *** presented codes can correct single errors and random asymmetric(1 → 0) errors within a b-bit byte. Unlike classical codes, these codes use integer and lookup table operations. As a result, their interleaved version, implemented on a dual-core 3.0 GHz processor, achieves the theoretical throughput above the operating rate of 10 G networks.

关键词： Asymmetric errors decoding throughput integer codes optical networks single errors