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Steady-state kinetics of <i>Roystonea regia</i>palm tree peroxidase

Journal of Biophysical Chemistry 2012年第1期3卷 16-28页

作者： Laura Sánchez zamorano Nazaret Hidalgo Cuadrado Patricia Pérez Galende Manuel G. Roig Valery L. Shnyrov Departamento de Química Física Facultad de Química Universidad de Salamanca Salamanca Spain

Royal palm tree peroxidase (RPTP) has been isolated to homogeneity from leaves of Roystonea regia palm trees. The enzyme purification steps included homogenization, (NH4)SO4 precipitation, extraction of palm leaf colored compounds and consecutive chromatography on Phenyl-Sepharose, TSK-Gel DEAE-5PW and Superdex-200. The novel peroxidase was characterized as having a molecular weight of 48.2 ± 3.0 kDa and an isoelectric point pI 5.4 ± 0.1. The enzyme forms dimers in solution with approximate molecular weight of 92 ± 2 kDa. Here we investigated the steady-state kinetic mechanism of the H2O2-supported oxidation of different organic substrates by RPTP. The results of the analysis of the initial rates vs. H2O2 and reducing substrate concentrations were seen to be consistent with a substrate-inhibited Ping-Pong Bi-Bi reaction mechanism. The phenomenological approach used expresses the peroxidase Ping-Pong mechanism in the form of the Michaelis-Menten equation and affords an interpretation of the effects in terms of the kinetic parameters KmH2O2, KmAH2, kcat, KSIH2O2, KSIAH2 and of the microscopic rate constants k1 and k3 of the shared three-step peroxidase catalytic cycle. Furthermore, the concentration and time-dependences and the mechanism of the suicide inactivation of RPTP by hydrogen peroxide were studied kinetically with guaiacol as co-substrate. The turnover number (r) of H2O2 required to complete the inactivation of the enzyme was 2154 ± 100 and the apparent rate constants of catalysis 185 s–1 and 18 s–1.

关键词： Roystonea regia Peroxidase Steady-State Kinetics Substrate Inhibition Mechanism-Based Inactivation Kinetics Hydrogen Peroxide

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Exploring the Role of Nutraceuticals (Red Yeast Rice) in Secondary Prevention: A New Pathway Can Be Opened

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World Journal of Cardiovascular Diseases 2022年第1期12卷 24-29页

作者： Santiago de Dios Eduardo Fernandez Carrion Javier Antona Makoshi Juan Jose Parra Fuertes Hector Fleites Eduardo Zatarain Jose Luis zamorano Gomez Department of Cardiology Hospital Universitario de la Zarzuela Madrid Spain Department of Maths Universidad Complutense Madrid Spain Department of Cardiology Hospital Conde Valle-Suchil Madrid Spain Department of Cardiology Hospital 12 de Octubre Madrid Spain Department of Cardiology Hospital Gregorio Mara&ntilde ón Madrid Spain

Background: Long-term survival in acute coronary syndrome has increased steadily in the last decades. Follow-up studies developed in this patient clearly reveal that they are at risk of suffering a new event, placing them in a new stage, secondary prevention. Assuming this increased risk, the control target of their cardiovascular risk factors become more ambitious. In this field, control of Cholesterol levels, particularly LDL-C, has arisen as a priority objective in patients with coronary arterial disease. In this sense, management of dyslipidemia guidelines, recently recognises the role of functional food, highlighting among them is the Red Yeast Rice (RYR). The aim of the study is to establish the potential role of functional food, in secondary prevention, while determining its additional capacity to reduce LDL-C in patients that despite optimal classic treatment (maximum tolerated dose of stain plus Ezetimibe) is still out of control objectives. Results and Discussion: 88 patients were included and after 3 months of tr

关键词： Lipids Nutraceuticals Coronary Artery Disease Secondary Prevention

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New environmental factors related to diabetes risk in humans:Emerging bisphenols used in synthesis of plastics

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World Journal of Diabetes 2023年第8期14卷 1301-1313页

作者： Rafael Moreno-Gómez-Toledano María Delgado-Marín Alberto Cook-Calvete Claudia González-Cucharero Nunzio Alcharani Beatriz Jiménez-Guirado Ignacio Hernandez Rafael Ramirez-Carracedo Laura Tesoro Laura Botana Sandra Sánchez-Esteban Javier Diez-Mata Jose Luis zamorano Ricardo J.Bosch Carlos Zaragoza Marta Saura Universidad de Alcalá Department of Biological Systems/PhysiologyAlcaláde Henares 28871Spain Cardiology Department University Hospital Ramón y CajalMadrid 28034Spain Laboratory of Cardiovascular Pathophysiology Joint Translational Research UnitUniversity Francisco de Vitoria School of MedicineMadrid 28034Spain Instituto Ramón y Cajal de Investigación Sanitaria-IRYCIS Madrid 28034Spain

BACKGROUND Diabetes mellitus(DM)is one of the largest global health emergencies of the 21st *** recent years,its connection with environmental pollutants,such as bisphenol A(BPA),has been demonstrated;consequently,new structurally similar molecules are used to replace BPA in the plastics industry(BPS,BPF and BPAF).AIM To carry out a systematic review to allow coherent evaluation of the state of the ***,a meta-analysis was performed to unify the existing quantitative *** Firstly,a systematic review was carried out,using the terms“(bisphenol)AND(Diabetes OR Hyperglycemia)”,to maximize the number of ***,three authors analyzed the set of ***,a meta-analysis was performed for each BP,using RevMan *** addition,funnel plots were developed to study publication *** The systematic analysis of the literature revealed 13 recent articles(2017–2023)related to the study *** qualitative analysis showed interesting data linking diabetes to the three most widely used substitute BPs in the industry:BPS,BPF and ***,the meta-analysis determined a positive relationship with BPS,BPF and BPAF,which was only statistically significant with *** There is a need to apply the precautionary principle,regulating the use of new ***,replacing BPA with BPS,BPF or BPAF is unlikely to protect the population from potential health risks,such as DM.

关键词： Bisphenol S Bisphenol F Bisphenol AF Diabetes mellitus Systematic review Meta-analysis

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在经皮球囊二尖瓣成形术中非侵入性评估二尖瓣面积:实时3D超声心动图的作用

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世界核心医学期刊文摘（心脏病学分册） 2005年第5期1卷 32-33页

作者： zamorano J. Perez De Isla L. 高登峰

In the last decade, multiple studies depicted discrepancies between mitral valvular orifice area(MVA) measurements obtained with the pressure half-time(PHT) method and invasive methods during the immediate post-percutaneous mitral valvuloplasty(PMV) period. Our aim was to assess the accuracy of Real-Time 3D echo(RT3D) to measure the MVA in the immediate post-PMV period. The invasively determined MVA was used as the gold standard. We studied 29 patients with rheumatic mitral stenosis from two centres(27 women; mean age 48.2±11.3 years), all of which had underwent PMV. MVA was calculated before and after PMV using the PHT method, 2D echo planime try, RT3D echo planimetry and invasive determination (Gorlin’s method). The RT3D MVA assessment showed a better agreement with the invasively derived MVA before and in the immediate post-PMV period (Bland-Altman analysis: Average difference between both methods and limits of agreement: 0.01(-0.31 to 0.33) cm2 and -0.12(-0.71 to 0.47) cm2) before and immediately after the PMV, respectively. RT3D is a feasible and accurate technique for measuring MVA in patients with RMVS. It has the best agreement with the invasively determined MVA, particularly in the immediate post-PMV period.

关键词：二尖瓣面积超声心动图瓣口面积金指标面积法

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墨西哥青少年睡眠时间与血压的关系

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中华高血压杂志(中英文) 2021年第2期29卷 198-198页

作者： Jansen EC Dunietz GL Matos-Moreno A Solano M Lazcano-Ponce E Sánchez-zamorano LM 周卫叶鹏

全球有5%的儿童患有高血压,并预示着以后心血管病的发病率。关于成人睡眠不足与高血压的关系报道较多,但鲜有在儿童中进行研究的报道。本研究是在一个大型墨西哥儿童队列中分析晚睡(睡眠时间短的标志)和潜在的昼夜节律异常在偶发性高血...

全球有5%的儿童患有高血压,并预示着以后心血管病的发病率。关于成人睡眠不足与高血压的关系报道较多,但鲜有在儿童中进行研究的报道。本研究是在一个大型墨西哥儿童队列中分析晚睡(睡眠时间短的标志)和潜在的昼夜节律异常在偶发性高血压中的作用。方法:纳入2 033名来自墨西哥莫雷洛斯公立学校的无血压升高青少年,即血压<同性别、年龄和身高青少年血压的第90百分位数的青少年。

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在有症状的患者中,与传统危险因素相比,冠状动脉钙化积分能更准确地预测显著的冠状动脉狭窄:欧洲钙化性冠状动脉疾病研究

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中华高血压杂志 2016年第3期24卷 206-206页

作者：刘莉叶鹏 Nicoll R Wiklund U Zhao Y Diederichsen A Mickley H Ovrehus K zamorano P Gueret P Schmermund A Maffei E Cademartiri F Budoff M Henein M

在这项回顾性研究中,研究人员评估了冠状动脉钙化积分相对于传统危险因素对显著狭窄（冠状动脉狭窄〉50%）的预测价值。方法与结果：纳入来自丹麦、法国、德国、意大利、西班牙和美国有症状的患者5515例,所有研究对象均进行危险因素评... 详细信息

在这项回顾性研究中,研究人员评估了冠状动脉钙化积分相对于传统危险因素对显著狭窄（冠状动脉狭窄〉50%）的预测价值。方法与结果：纳入来自丹麦、法国、德国、意大利、西班牙和美国有症状的患者5515例,所有研究对象均进行危险因素评估、计算机断层冠状动脉造影（computed tomographic coronary angiogram,CTCA）或常规血管造影。

关键词：冠状动脉钙化传统危险因素钙化性危险因素评估冠状动脉造影预测价值血管造影 tomographic 高胆固醇血症多元回归分析

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首次心肌梗死前二尖瓣反流对预后的影响

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世界核心医学期刊文摘（心脏病学分册） 2005年第8期1卷 47-47页

作者： zamorano J. Perez De Isla L. Oliveros L. 郭战宏 Echocardiogr./Cardiovasc. I. L. Hospital Clinico San Carlos Plaza de Cristo Rey 28040 Madrid Spain

Aims Mitral regurgitation(MR) following an acute myocardial infarction(AMI) confers an adverse prognosis during long term follow- up. There are no studies evaluating the influence of pre- AMI MR in the short- and long- term prognosis of such patients. Our aim was to assess the prognostic value of pre- AMI MR in the short and long- term follow- up of patients who suffered a first AMI and to assess its influence on left ventricular haemodynamics. Methods and results Sixty- eight consecutive patients with a first AMI and an echocardiographic study before AMI(< 3 months) were included in the study. The pre- AMI echo was performed for various reasons. Of these 68 patients, 42 had pre- AMI MR(Group 1) and 26 showed no pre- AMI MR(Group 2). The presence of degenerative changes at the level of the mitral valve was confirmed in all cases. Patients with any other cause of MR were excluded. Clinical and echocardiographic variables for both phases(pre- AMI and post- AMI) were analysed and patients were followed up. Mean age was 75.5± 9.5 years; there were 38 males(55.9% ). There were no statistical differences in baseline clinical variables between the groups, except for the presence of pre- AMI atrial fibrillation, which was more frequent in Group 1(21.4 vs. 0% ; P=0.01 ). After AMI, only end- diastolic left ventricular diameter was significantly larger in Group 1(54.9± 4.7 vs. 48.1± 5.6 mm; P< 0.001). During long- term follow- up, median survival times were 912 days(interquartile range: 690 days) in Group 1 and 1423 days(interquartile range: 520 days) in Group 2(Log- rank P=0.02). The multi- variable analysis showed that the presence of pre- AMI MR relates to a statistically significant relationship with a worse post- AMI evolution[relative risk(95% confidence interval): 3.8(1.1- 13.1); P=0.037]. Conclusion The present study shows that the presence of pre- AMI MR is an independent prognostic marker among those patients suffering a first AMI.

关键词：二尖瓣反流超声检查超声心动图血流动力学预后预测中位生存时间临床参数预后指标四分位数多变量分析

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首次非ST段抬高型急性冠状动脉综合征后功能性二尖瓣反流的预后意义

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世界核心医学期刊文摘(心脏病学分册) 2007年第5期 24-24页

作者： Perez De Isla L. zamorano J. Quezada M. 韩瑞娟 Instituto Cardiovascular Echocardiographic Laboratory Hospital Clnico San Carlos Plaza Cristo Rey 28040 Madrid Spain

目的:急性心肌梗死(AMI)后发生二尖瓣反流(MR)已被公认为是一种常见的并发症,其对存活率的负面影响已有观察。然而,关于非ST段抬高型急性冠状动脉综合征(NSTSEACS)后发生MR的资料少见。本研

关键词：急性冠状动脉综合征预后意义 LVEF ST

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急性心肌梗死后参数成像定量心肌声学造影识别心肌顿抑

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世界核心医学期刊文摘（心脏病学分册） 2005年第12期1卷 28-28页

作者： Serra V. Perez De Isla L. Ferro M. P. J. zamorano 杨海涛 Instituto Cardiovascular Hospital Clnico San Carlos Madrid Spain Dr.

Microvascular integrity demonstrated by myocardial contrast echocardiography(MCE) predicts functional recovery after an acute myocardial infarction(AMI). Recently, parametric imaging-based quantitative MCE has been developed. Our aim was to assess the usefulness of parametric imaging-based quantitative MCE parameters to predict the functional recovery of akinetic segments after primary percutaneous transluminal coronary angioplasty(PTCA). Fifty-three consecutive patients with a first AMI were enrolled. They underwent primary PTCA. Standard echocardiography and real-time MCE were performed. Qualitative analysis and parametric imaging-based quantitative parameters were measured offline by different blinded investigators. Dobutamine stress echocardiography was performed 1 month later. A new standard echocardiogram to assess the functional status of the akinetic segments and coronary angiography to evaluate the presence of restenosis at the level of the culprit lesion were performed 6 months later. The mean patient age was 62.9±14 years, and 42 were men(79%); 170 segments were akinetic. Of these, 105(62%) recovered their function. The best parameter to predict functional recovery was the myocardial blood flow velocity(β). These results were better than those obtained using dobutamine stress echocardiography and qualitative MCE to predict functional recovery. In conclusion, parametric imaging-based quantitative MCE is an accurate diagnostic tool to detect stunned myocardium after AMI. Its diagnostic accuracy in predicting the functional recovery of akinetic segments after primary PTCA is better than the accuracy of dobutamine stress echocardiography and qualitative MCE.

关键词：心肌声学造影急性心肌梗死心肌顿抑负荷超声心动图心肌节段冠状动脉造影再狭窄多巴酚丁胺心肌血流功能恢复情况

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