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The effects of soil properties, cropping systems and geographic location on soil prokaryotic communities in four maize production regions across China

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Journal of integrative Agriculture 2022年第7期21卷 2145-2157页

作者： TiAN Xue-liang LiU Jia-jia LiU Quan-cheng XiA Xin-yao PENG Yong alejandra i.huerta YAN Jian-bing Li Hui LiU Wen-de State Key Laboratory for Biology of Plant Diseases and insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Henan Engineering Research Center of Biological Pesticide&Fertilizer Development and Synergistic Application Henan Institute of Science and TechnologyXinxiang 453003P.R.China School of Biological Science and Technology University of JinanJinan 250022P.R.China National Key Laboratory of Crop Genetic improvement Huazhong Agricultural UniversityWuhan 430070P.R.China Department of Entomology and Plant Pathology North Carolina State UniversityRaleigh 27606USA

The diversity of prokaryotic communities in soil is shaped by both biotic and abiotic ***,little is known about the major factors shaping soil prokaryotic communities at a large scale in *** this end,we undertook a study to investigate the impact of maize production cropping systems,soil properties and geographic location(latitude and longitude)on soil prokaryotic communities using metagenomic techniques,across four distinct maize production regions in *** all study sites,the dominant prokaryotes in soil were Alphaproteobacteria,Gammaproteobacteria,Betaproteobacteria,Gemmatimonadetes,Acidobacteria,and ***-metric multidimensional scaling revealed that prokaryotic communities clustered into the respective maize cropping systems in which they *** analysis(RDA)showed that soil properties especially pH,geographic location and cropping system jointly determined the diversity of the prokaryotic *** functional genes of soil prokaryotes from these samples were chiefly influenced by latitude,soil pH and cropping system,as revealed by RDA *** abundance of genes in some metabolic pathways,such as genes involved in microbe–microbe interactions,degradation of aromatic compounds,carbon fixation pathways in prokaryotes and microbial metabolism were markedly different across the four maize production *** study indicated that the combination of soil pH,cropping system and geographic location significantly influenced the prokaryotic community and the functional genes of these *** work contributes to a deeper understanding of the composition and function of the soil prokaryotic community across large-scale production systems such as maize.

关键词： metagenome cropping system maize soil prokaryotes

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An optimized protocol for stepwise optimization of real-time RT-PCR analysis

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Horticulture Research 2021年第1期8卷 2474-2494页

作者： Fangzhou Zhao Nathan A.Maren Pawel Z.Kosentka Ying-Yu Liao Hongyan Lu James R.Duduit Debao Huang Hamid Ashrafi Tuanjie Zhao alejandra i.huerta Thomas G.Ranney Wusheng Liu Soybean Research institute Nanjing Agricultural University210095 NanjingChina Department of Horticultural Science North Carolina State UniversityRaleighNC 27607USA Mountain Crop improvement Lab Department of Horticultural ScienceMountain Horticultural Crops Research and Extension CenterNorth Carolina State UniversityMills RiverNC 28759USA Department of Entomology and Plant Pathology North Carolina State UniversityRaleighNC 27607USA College of Biosystems Engineering and Food Science Zhejiang University310058 HangzhouChina

Computational tool-assisted primer design for real-time reverse transcription(RT)PCR(qPCR)analysis largely ignores the sequence similarities between sequences of homologous genes in a plant *** can lead to false confidence in the quality of the designed primers,which sometimes results in skipping the optimization steps for ***,the optimization of qPCR parameters plays an essential role in the efficiency,specificity,and sensitivity of each gene’s ***,we proposed an optimized approach to sequentially optimizing primer sequences,annealing temperatures,primer concentrations,and cDNA concentration range for each reference(and target)*** approach started with a sequence-specific primer design that should be based on the single-nucleotide polymorphisms(SNPs)present in all the homologous sequences for each of the reference(and target)genes under *** combining the efficiency calibrated and standard curve methods with the 2−ΔΔCt method,the standard cDNA concentration curve with a logarithmic scale was obtained for each primer pair for each *** a result,an R 2≥0.9999 and the efficiency(E)=100±5% should be achieved for the best primer pair of each gene,which serve as the prerequisite for using the 2^(−ΔΔCt) method for data *** applied our newly developed approach to identify the best reference genes in different tissues and at various inflorescence developmental stages of Tripidium ravennae,an ornamental and biomass grass,and validated their utility under varying abiotic stress *** also applied this approach to test the expression stability of six reference genes in soybean under biotic stress treatment with Xanthomonas axonopodis ***(Xag).Thus,these case studies demonstrated the effectiveness of our optimized protocol for qPCR analysis.

关键词： optimization analysis stepwise

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The Beneficial Effects of Components of Garlic (Allium sativum L.) in the Poultry industry

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Food and Nutrition Sciences 2024年第1期15卷 27-57页

作者： alejandra Meza-Rios Gilberto Velazquez-Juarez inkar Castellanos-huerta Rocio ivette Lopez-Roa Luis A. Anguiano-Sevilla Xochitl Hernanez-Velasco Xochitl Hernanez-Velasco Saeed El-Ashram Ebtsam Al-Olayan Billy Hargis Guillermo Tellez-isaias Adelaida Sara Minia Zepeda-Morales Departamento de Farmacobiología CUCEI Universidad de Guadalajara Blvd. M. García Barragán Guadalajara México Laboratorio de Análisis Fisicoquímicos Externos Departamento de Química CUCEI Universidad de Guadalajara Boulevard Marcelino García Barragán Guadalajara México Center of Excellence for Poultry Science Department of Poultry Science Dale Bumpers College of Agricultural Food and Life Sciences University of Arkansas Cumberland United States Laboratorio de investigación y Desarrollo Farmacéutico Departamento de Farmacobiología CUCEI Universidad de Guadalajara Boulevard Marcelino García Barragán Guadalajara México Departamento de Medicina y Zootecnia de Aves Facultad de Medicina Veterinaria y Zootecnia UNAM Mexico Mexico Zoology Department Faculty of Science Kafrelsheikh University Kafr El-Sheikh Egypt Department of Zoology College of Science King Saud University Riyadh Saudi Arabia Laboratorio de Análisis Clínicos y Bacteriológicos (vinculación) Departamento de Farmacobiología CUCEI Universidad de Guadalajara Boulevard Marcelino García Barragán Guadalajara México

Plant-based antimicrobial agents are readily available, cost-effective, and exhibit low toxicity, making them promising alternatives in combatting microbial infections. Among these plants, garlic (Allium sativum) stands out for its traditional medicinal use in effectively combating various microorganisms. in the poultry industry, preventing avian virus and bacterial infections is paramount for chicken husbandry. However, using conventional drugs poses potential risks to human health. Garlic, a widely used Asian plant in traditional medicine for various pathologies, has shown potential as an herbal prophylactic remedy against viral and bacterial infections. Recently, researchers explored garlic and its derivatives as a scientific strategy in veterinary practices for diverse purposes, such as improving poultry production characteristics and acting as antibiotic growth promoters. This comprehensive review delves into garlic and its derivatives as preventive and corrective treatments for viral diseases in laying hens and broilers. The paper highlights their potential effectiveness and safety as a natural means to enhance poultry health and welfare while mitigating the risks associated with conventional drug usage in the food industry.

关键词： Garlic Antiviral Veterinary Chickens Allium sativum

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The Beneficial Effects of Components of Garlic (Allium sativum L. ) in the Poultry industry

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Food and Nutrition Sciences（食品与营养科学） 2024年第1期

作者： alejandra Meza-Rios Gilberto Velazquez-Juarez inkar Castellanos-huerta Rocio ivette Lopez-Roa Luis A.Anguiano-Sevilla Xochitl Hernanez-Velasco Saeed El-Ashram Ebtsam Al-Olayan Billy Hargis Guillermo Tellez-isaias Adelaida Sara Minia Zepeda-Morales

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Potential Species Distribution and Richness of ixodidae Ticks Associated with Wild Vertebrates from Michoacán, Mexico

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Journal of Geographic information System 2014年第5期6卷 467-477页

作者： Margarita Vargas-Sandoval Angel G. Priego-Santander alejandra Larrazábal Carolina G. Sosa-Gutiérrez Blanca Lara-Chávez Teresita Avila-Val Facultad de Agrobiología “Presidente Juárez” Universidad Michoacana de San Nicolás de Hidalgo Uruapan México Centro de investigaciones en Geografía Ambiental UNAM Antigua Carretera a Pátzcuaro No. 8701 ExHacienda de San José de la Huerta Morelia México Unidad de investigación Médica de Enfermedades infecciosas y Parasitarias Hospital de Pediatría Centro Médico Nacional Siglo XXI México DF México

Ticks are regarded as the most relevant vectors of disease-causing pathogens in domestic and wild animals. The diversity of ixodidae is known for a very small number of ***?are represented by 26 species, and?in?2007 the first reported ticks vectors in Mexico for the causal agent of Lyme disease. Recent rise in tick-borne disease in many parts of the world is a phenomenon in need of an explanation. The main objective of the present work was to map at a regional scale (1:2,000,000)?of?the distribution of ticks of the family ixodidae that are potentially present on the wild fauna of state of Michoacan, Mexico. We compiled all available literature on ticks at a national level together with complete cartographic and bibliographic georeferenced information of the distribution of hosts in order to build a spatial database in ArcView 3.3. The results indicated that the wild fauna in the state’s territory could potentially include 31 species of ticks of ixodidae. The map of potential species richness of ixodid ticks on the wild fauna of the state can be categorized into five classes of species richness. The highest potential concentration of ixodid species on wild fauna occurs in the volcanic area of Pico de Tancítaro.

关键词： Ticks ixodidae Michoacán Geographic Distribution Potential Species Richness

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