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Debaryomyces hansenii supplementation in low fish meal diets promotes growth,modulates microbiota and enhances intestinal condition in juvenile marine fish

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Journal of Animal Science and Biotechnology 2024年第1期15卷 253-276页

作者： Ignasi Sanahuja Alberto ruiz Joana P.Firmino Felipe E.reyes-López Juan B.Ortiz-Delgado Eva Vallejos-Vidal Lluis Tort Dariel Tovar-Ramírez Isabel M.Cerezo Miguel A.Moriñigo Carmen Sarasquete Enric Gisbert Aquaculture Program Institute of Agrifood Research and Technology(IRTA)La Rapita 43540Spain Centro de Biotecnologia Acuicola Universidad de Santiago de ChileSantiagoChile Instituto de Ciencias Marinas de Andalucia(ICMAN-CSIC) Avda.Republica Saharaui no 2Campus Universitario Rio San PedroPuerto RealCadiz 11510Spain Nucleo de Investigaciones Aplicadas en Ciencias Veterinarias y Agronomicas Facultad de Medicina Veterinaria y AgronomiaUniversidad de Las AmericasSantiagoChile Department of Cell Biology Physiologyand ImmunologyUniversitat Autonoma de BarcelonaBarcelonaSpain Centro de Investigaciones Biologicas del Noroeste SC CIBNORLa PazMexico Department of Microbiology Instituto de Biotecnologia Y Desarrollo Azul(IBYDA)Faculty of SciencesUniversity of Malaga29010 MalagaSpain SCBI Bioinformatic UnitUniversity of Malaga29590 MalagaSpain

Background The development of a sustainable business model with social acceptance,makes necessary to develop new strategies to guarantee the growth,health,and well-being of farmed *** hansenii is a yeast species that can be used as a probiotic in aquaculture due to its capacity to i)promote cell proliferation and differen-tiation,ii)have immunostimulatory effects,iii)modulate gut microbiota,and/or iv)enhance the digestive *** provide inside into the effects of *** on juveniles of gilthead seabream(Sparus aurata)condition,we inte-grated the evaluation of the main key performance indicators coupled with the integrative analysis of the intestine condition,through histological and microbiota state,and its transcriptomic *** After 70 days of a nutritional trial in which a diet with low levels of fishmeal(7%)was supplemented with 1.1%of ***(17.2×10^(5) CFU),an increase of ca.12%in somatic growth was observed together with an improve-ment in feed conversion in fish fed a yeast-supplemented *** terms of intestinal condition,this probiotic modu-lated gut microbiota without affecting the intestine cell organization,whereas an increase in the staining intensity of mucins rich in carboxylated and weakly sulphated glycoconjugates coupled with changes in the affinity for certain lectins were noted in goblet *** in microbiota were characterized by the reduction in abundance of several groups of Proteobacteria,especially those characterized as opportunistic *** microarrays-based transcrip-tomic analysis found 232 differential expressed genes in the anterior-mid intestine of ***,that were mostly related to metabolic,antioxidant,immune,and symbiotic *** Dietary administration of *** enhanced somatic growth and improved feed efficiency param-eters,results that were coupled to an improvement of intestinal condition as histochemical and transcriptomic tools *** probiotic ye

关键词： Debaryomyces hansenii Intestine condition Low fish meal diet Microbiota Transcriptomics Yeast probiotic

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Organ Abscission in Plants: With Special Emphasis on Bell Pepper

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Phyton-International Journal of Experimental Botany 2024年第7期93卷 1467-1484页

作者： Ramiro reyes-Castro Héctor G.Núñez-Palenius Juan I.Valiente-Banuet María E.Sosa-Morales Blanca E.Orosco-Alcalá Rafael Guzmán-Mendoza Graciela M.L.ruiz-Aguilar Rogelio Costilla-Salazar Life Sciences Division Universidad de GuanajuatoIrapuato36500México School of Engineering and Sciences Tecnologico de MonterreyQuerétaro76130México

Bell pepper(Capsicum annuum L.),along with potato and tomato,is one of the three most cultivated vegetables in the *** pepper is worldwide accepted due to its characteristics of color,smell,ﬂavor,and *** pepper is also considered a nutritious food due to its vitamin and antioxidant *** Mexico,bell pepper production has a high value because it is grown for the international markets,particularly the United States of ***,the abscission ofﬂowers and fruits is a factor that limits the yield,hinders the planning of activities,and causes a variation in the prices of *** to the importance of this process,in this work we did a bibliometric analysis and literature review of scientiﬁc advances for the understanding of the abscission pro-cess in plants,and in particular for the bell ***,we introduce new perspectives that would help direct future research about organ abscission in ***,the lack of research that would further clarify abscis-sion process in the bell pepper plant is discussed.

关键词： Capsicum annuum abscission zone plant growth regulators environmental factors

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Microbial symphony orchestrated by mucosal IgA

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Cellular & Molecular Immunology 2022年第4期19卷 456-457页

作者： alejand.a reyes-ruiz Jordan D.Dimitrov Centre de Recherche des Cordeliers INSERMCNRSSorbonne UniversitéUniversitéde Paris75006ParisFrance

The ability of an organism to handle the beauty and cruelty of existence is largely due to a complex network of molecular and cellular interactions and processes named the immune *** components of this network are antibodies(Abs)that have key roles in immune defense and *** biological functions of Abs include neutralizing pathogens by blocking functionally important molecules and/or to eliciting lethal effector functions by bridging the recognition of pathogens with binding to different innate immune receptors.

关键词： bridging neutral network

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Increased DNA binding activity of NF-κB,STAT-3,SMAD3 and AP-1 in acutely damaged liver

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World Journal of Gastroenterology 2006年第37期12卷 5995-6001页

作者： Adriana Salazar-Montes Luis ruiz-Corro Ana Sandoval-Rodriguez Alberto Lopez-reyes Juan Armendariz-Borunda Institute of Molecular Biology in Medicine and Gene Therapy CUCS University of Guadalajara Apdo. Postal 2-123 Guadalajara 44281 Jal Mexico Institute of Molecular Biology in Medicine and Gene Therapy CUCS University of Guadalajara Apdo.Postal 2-123 Guadalajara 44281 Jal Mexico OPD Hospital Civil de Guadalajara Guadalajara Jal Mexico

AIM: To investigate the role of genes and kinetics of specific transcription factors in liver regeneration, and to analyze the gene expression and the activity of some molecules crucially involved in hepatic regeneration. METHODS: USING gel-shift assay and RT-PCR, transcription factors, such as NF-κB, STAT-3, SMAD3 and AP-1, and gene expression of inducible nitric oxide synthase (iNOS), hepatocyte growth factor (HGF) and c-met were analyzed in an animal model of chemically induced hepatectomy. RESULTS: Gene expression of HGF and its receptor c-met peaked at 3 h and 24 h after acute CCl4 intoxi- cation. iNOS expression was only observed from 6 to 48 h. Transcriptional factor NF-κB had an early activation at 30 min after acute liver damage. STAT-3 peaked 3 h post- intoxication, while AP-1 displayed a peak of activation at 48 h. SMAD3 showed a high activity at all analyzed times. CONCLUSION: TNF-α and IL-6 play a central role in hepatic regeneration. These two molecules are responsible for triggering the cascade of events and switch-on of genes involved in cell proliferation, such as growth factors, kinases and cyclins which are direct participants of cell proliferation.

关键词： Hepatic regeneration Transcription factor Gene regulation

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Effect of the initial ECAP passes on crystal texture and residual stresses of 5083 aluminum alloy

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International Journal of Minerals,Metallurgy and Materials 2020年第6期27卷 801-808页

作者： L.Romero-Reséndiz A.Flores-Rivera I.A.Figueroa C.Braham C.reyes-ruiz I.Alfonso G.González Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de MéxicoCd.de México 04510México Procédés et Ingénierie en Mécanique et Matériaux Arts et MétiersParis 75013France Departamento de Diseño y Manufactura Facultad de IngenieríaUniversidad Nacional Autónoma de MéxicoPIIT Apodaca 66629México Instituto de Investigaciones en Materiales Unidad MoreliaUniversidad Nacional Autónoma de MéxicoMorelia 58190México

To produce a highly refined microstructure,several metals or alloys have been processed via equal-channel angular pressing(ECAP).In this work,the mechanical and microstructural changes of the 5083 aluminum alloy in H11 condition after processed by two ECAP passes were *** ECAP H13 steel die with an inner angle(α)of 120°and outer curvature(β)of 20°was *** microstructural changes were associated with the loss of texture *** morphologies of the Mg2Si andα-Al(Mn,Fe)Si precipitates for the sample at the initial condition were similar to those subjected to two ECAP *** peak broadening measured by X-ray diffraction revealed an increment of both grain refinement and *** the second extrusion pass,the hardness increased by 62%compared with the initial ***,the heterogeneous hardness behavior was compatible with a highly localized dislocation *** two ECAP passes,shear parallel bands were observed to be at nearly 45°to the extrusion *** evaluation of first-order residual stress as a function of the depth of the analyzed sample displayed compressive or tensile values,depending on the measured *** the plastic deformation applied,the first and second-order residual stresses exhibited significant ***-Hall plots showed positive slopes,indicating an increment in the microstrain.

关键词： aluminum alloy equal-channel angular pressing texture microstructure residual stress

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Application of formal languages in polynomial transformations of instances between NP-complete problems

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Journal of Zhejiang University-Science C(Computers and Electronics) 2013年第8期14卷 623-633页

作者： Jorge A. ruiz-VANOYE Joaquín PREZ-ORTEGA Rodolfo A. PAZOS RANGEL Ocotlán DíAZ-PARRA Héctor J. FRAIRE-HUACUJA Juan FRAUSTO-SOLíS Gerardo reyes-SALGADO Laura CRUZ-reyes DACI Universidad Autónoma del Carmen Computer Science Centro Nacional de Investigación y Desarrollo Tecnológico Systems and Computer Science Instituto Tecnológico de Ciudad Madero Informatics Universidad Politécnica del Estado de Morelos Computer Science Instituto Tecnológico de Cuautla

We propose the usage of formal languages for expressing instances of NP-complete problems for their application in polynomial transformations. The proposed approach, which consists of using formal language theory for polynomial transformations, is more robust, more practical, and faster to apply to real problems than the theory of polynomial transformations. In this paper we propose a methodology for transforming instances between NP-complete problems, which differs from Garey and Johnson's. Unlike most transformations which are used for proving that a problem is NP-complete based on the NP-completeness of another problem, the proposed approach is intended for extrapolating some known characteristics, phenomena, or behaviors from a problem A to another problem B. This extrapolation could be useful for predicting the performance of an algorithm for solving B based on its known performance for problem A, or for taking an algorithm that solves A and adapting it to solve B.

关键词： Formal languages Polynomial transformations NP-completeness

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Human CD4 ＋ effector T lymphocytes generated upon TCR engagement with self-peptides respond defectively to IL-7 in their transition to memory cells

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Cellular & Molecular Immunology 2013年第3期10卷 261-274页

作者： Gabriela Gonzalez-Perez Norma C Segovia Amaranta Rivas-Carvalho Diana P reyes Honorio Torres-Aguilar Sergio R Aguilar-ruiz Claudine Irles Gloria Soldevila Carmen Sainchez-Torres 1Department of Molecular Biomedicine Centre de Investigacion y de Estudios Avanzados del I.P.N. (CINVESTAV-IPN) Mexico City Mexico Department of Physiology Facultad de Medicina Mexico City Mexico Department of Immunology Institute de Investigaciones Biomedicas Universidad Nacional Autonoma de Mexico (UNAM) Mexico City Mexico

The peripheral repertoire of CD4＋ T lymphocytes contains autoreactive cells that remain tolerant through several mechanisms. However, nonspecific CD4＋ T cells can be activated in physiological conditions as in the course of an ongoing immune response, and their outcome is not yet fully understood. Here, we investigate the fate of human naive CD4＋ lymphocytes activated by dendritic cells （DCs） presenting endogenous self-peptides in comparison with lymphocytes involved in alloresponses. We generated memory cells （Tmem） from primary effectors activated with mature autologous DCs plus interleukin （IL）-2 （Tmauto）, simulating the circumstances of an active immune response, or allogeneic DCs （Tmallo）. Tmem were generated from effector cells that were rested in the absence of antigenic stimuli, with or without IL-7. Tmem were less activated than effectors （demonstrated by CD25 downregulation） particularly with IL-7, suggesting that this cytokine may favour the transition to quiescence. Tmauto and TmaHo showed an effector memory phenotype, and responded similarly to polyclonal and antigen-specific stimuli. Biochemically, IL-7-treated Tma^o were closely related to conventional memory lymphocytes based on Erk-l/2 activation, whereas Tmauto were more similar to effectors. Autologous effectors exhibited lower responses to IL-7 than allogeneic cells, which were reflected in their reduced proliferation and higher cell death. This was not related to IL-7 receptor expression but rather to signalling deficiencies, according to STAT5 activation These results suggest that ineffective responses to IL-7 could impair the transition to memory cells of naive CD4＋ T lymphocytes recognizing self-peptides in the setting of strong costimulation.

关键词： human CD4+ T cells IL-7 memory self-peptides

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Characterization of two Achromobacter xylosoxidans isolates from patients with pertussis-like symptoms

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Asian Pacific Journal of Tropical Medicine 2015年第6期8卷 465-468页

作者： Fiorella Orellana-Peralta Michelle Jacinto Maria J.Pons Cláudia Gomes Carlos Bada Isabel reyes Juana del Valle Mendoza Joaquim ruiz Facultad de Ciencias de la Salud.Universidad Peruana de Ciencias Aplicadas-LiPC LimaPeru ISGlobal Barcelona Ctr.Int.Health Res.(CRESIB)Hospital Clinic-Universitat de Barcelona Hospital de Emergencias Pediatricas.Lima Peru Instituto de Investigacion Nutrirional LimaPeru

Objective:To characterize two Achromobaeter xylosoxidans recovered from 2 patients diagnosed with pertussis during a Bordetella pertussis surveillance ***:Nasopharyngeal swabs from 2 children under 1 year of age with clinical suspicion of pertussis were analyzed by culture and ***:Two Achromobaeter xylosoxidans A8,closely related to Bordetella *** recovered from 2 patients diagnosed of pertussis,both carrying the ptxA gene and IS418 the pertussis toxin encoding ***,antibiotic susceptibility was evaluated by disk-diffusion method and by ***:Although more detailed studies are needed,the present data highlight the possibility that Achromobaeter *** related Bordetella pertussis microorganisms and not covered under the vaccine umbrella,might also result in cases of whooping *** further surveillance is necessary to determine the extension and relevance of their pathogenic role in order to discriminate their real public health implication.

关键词： Achromobacter xylosoxidans Bordetella pertussis

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Selection and Analysis of Polymorphisms in Somaclonal Variants of Agave americana Resistant to Fusarium oxysporum via an Ethyl Methanesulphonate Treatment

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Phyton-International Journal of Experimental Botany 2021年第6期90卷 1727-1739页

作者： Sheila Jazmín reyes-Zambrano Carlos Alberto Lecona-Guzmán Federico Antonio Gutiérrez-Miceli Víctor Manuel ruiz-Valdiviezo Arely Anayansi Vargas-Díaz Laboratorio de Biotecnología Vegetal Instituto Tecnológico de Tuxtla GutiérrezTecnológico Nacional de MéxicoTuxtla GutiérrezChiapas29050México CONACYT-Colegio de Postgraduados Campus Campeche ChampotónCampeche24450México

Agave americana *** were exposed to different concentrations of ethyl methanesulphonate(EMS)0,15,30,45 and 60 mM and to different times of exposure(2 and 4 h).The viability and capacity of shoot formation were shown to be affected when the callus were exposed to high concentrations(30–60 mM).Only the callus exposed to 15 mM EMS presented shoot formation;the exposure time of two hours produced the largest quantity of shoots regenerated per callus(21 shoots/callus).In order to generate somaclonal variants resistant to Fusarium oxysporum,a selection pressure was applied through of a culture filtrate(CF)of 100 ppm of the *** was made in callus obtained in the treatment with 15 mM EMS during 2 h of *** CF caused oxidation and necrosis in 71.25%of the callus;however,they were capable of generating shoots(3.5 shoots/callus).Molecular markers type RAPD,ISSR and DAMD were used to evaluate the genetic variation arising from the mutations caused by EMS on control plants and 16-month-old somaclonal *** polymorphic information content(PIC)for each one of the initiating groups was:0.28(DAMD),0.09(ISSR)and 0.14(RAPD).DAMD revealed a greater percentage of polymorphism than RAPD and *** bands were detected in the somaclonal *** indicated that the EMS caused genetic variation in the regenerated plants conferring resistance to them against Fusarium oxysporum.

关键词： Mutagenesis polymorphisms genetic variation molecular markers

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Insecticidal Activity and Physicochemical Characterization of Nanoparticles from Foliar Extract of Capsicum chinense

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Journal of Renewable Materials 2023年第11期11卷 3933-3943页

作者： Arnoldo E.Alfaro-Corres Daniel Gonzalez-Mendoza Esau ruiz-Sanchez Carlos Ail-Catzin Benjamin Valdez-Salas Federico Gutierrez-Miceli Arturo reyes-Ramirez Jacques Fils Pierre Division of Postgraduate Studies and Research Tecnologico Nacional de MéxicoCampus ConkalConkalYucatán97345Mexico Institute of Agricultural Sciences Universidad Autonoma de Baja CaliforniaEjido Nuevo LeonMexicaliBaja California21705Mexico Instituto de Ingeniería Universidad Autónoma de Baja CaliforniaMexicaliBaja California21100Mexico Division of Postgraduate Studies and Research Tecnologico Nacional de MexicoCampus Tuxtla GutierrezTuxtla GutierrezChiapas29050Mexico

This work was carried out to evaluate the insecticidal effect of mono,bi and trimetallic nanoparticles(NPs)from leaf extract of habanero pepper(Capsicum chinense Jacq.)on the red flour beetle(Tribolium castaneum herbst.)and cotton mealybug(Phenacoccus solenopsis Tinsley).The results showed that Cu NPs,Cu/Mn NPs and Zn/Cu NPs had high insecticidal effect against ***(63%-80% mortality after 96 h).The Cu NPs and Zn/Cu NPs showed also insecticidal effect against ***,but to a lesser extent(20%–28% after 96 h).With regards to the physicochemical characterization,Cu NPs had a zeta potential of 21.1 mV,whereas Cu/Mn NPs and Zn/Cu NPs exhibited zeta potentials of 200 *** polydispersity index(PDI)values for Cu NPs,Cu/Mn NPs,and Zn/Cu NPs were 10.1,29.16,and 14.34,*** TF-IR spectra of the NPs varied from 600 to 4000 cm−*** EDX weight percentages showed the presence of Cu(0.29%–2.4%),Mn(1.4%),and Zn(2.6%)in Cu/Mn NPs and Zn/Cu NPs,respectively.

关键词： Phytonanoparticles botanical insecticide pest control agronanotechnology

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