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A new oxidative pathway of nitric oxide production from oximes in plants

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Molecular Plant 2024年第1期17卷 178-198页

作者： Pedro lopez-Gomez Javier Buezo Marina Urra Alfonso Cornejo Raquel Esteban Jorge Fernandez de los reyes Estibaliz Urarte Estefania Rodriguez-Dobreva Alejandro Chamizo-Ampudia Alejandro Eguaras Sebastian Wolf Daniel Marino Victor Martinez-Merino Jose F.Moran Institute for Multidisciplinary Research in Applied Biology(IMAB) Department of SciencesPublic University of Navarre(UPNA)Avda.de Pamplona 12331192 MutilvaSpain Institute for Advanced Materials and Mathematics(INAMAT2) Department of SciencesPublic University of Navarre(UPNA)Campus de Arosadia31006 PamplonaSpain Department of Plant Biology and Ecology University of the Basque Country(UPV/EHU)Sarriena s/nApdo.64448080 BilbaoSpain Center for Plant Molecular Biology(ZMBP) University of TubingenGeschwister-Scholl-Platz72074 TubingenGermany Institute of Natural Resources and Agrobiology of Salamanca(IRNASA) Department of Plant-Microorganism InteractionsSpanish National Research Council(CSIC)Cordel de Merinas40-5237008SalamancaSpain Department of Biochemistry Area of Molecular BiologyVeterinary FacultyUniversity of LeonCampus de Vegazanas/n.24071 LeonSpain

Nitric oxide(NO)is an essential reactive oxygen species and a signal molecule in *** several studies have proposed the occurrence of oxidative NO production,only reductive routes for NO production,such as the nitrate(NO_(3)^(-))-upper-reductase pathway,have been evidenced to date in land ***,plants grown axenically with ammonium as the sole source of nitrogen exhibit contents of nitrite and NO3−,evidencing the existence of a metabolic pathway for oxidative production of *** hypothesized that oximes,such as indole-3-acetaldoxime(IAOx),a precursor to indole-3-acetic acid,are intermediate oxidation products in NO *** detected the production of NO from IAOx and other oximes catalyzed by peroxidase(POD)enzyme using both 4-amino-5-methylamino-2′,7′-difluorescein fluorescence and *** stimulated the reaction,while superoxide dismutase inhibited ***,mouse NO synthase can also use IAOx to produce NO at a lower rate than *** provided a full mechanism for POD-dependent NO production from IAOx consistent with the experimental data and supported by density functional theory *** showed that the addition of IAOx to extracts from Medicago truncatula increased the in vitro production of NO,while in vivo supplementation of IAOx and other oximes increased the number of lateral roots,as shown for NO donors,and a more than 10-fold increase in IAOx dehydratase ***,we found that in vivo supplementation of IAOx increased NO production in Arabidopsis thaliana wild-type plants,while prx33-34 mutant plants,defective in POD33-34,had reduced *** data show that the release of NO by IAOx,as well as its auxinic effect,explain the superroot ***,our study reveals that plants produce NO utilizing diverse molecules such as oximes,POD,and flavins,which are widely distributed in the plant kingdom,thus introducing a long-awaited oxidative pathway to NO production in plants.T

关键词： ammonium oxidation auxin nitric oxide oxime peroxidase root phenotype

Unilateral rNurr1-V5 transgene expression in nigral dopaminergic neurons mitigates bilateral neuropathology and behavioral deficits in parkinsonian rats withα-synucleinopathy

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Neural Regeneration Research 2024年第9期19卷 2057-2067页

作者： Bismark Gatica-Garcia Michael J.Bannon Irma Alicia Martínez-Dávila Luis O.Soto-Rojas David reyes-Corona Lourdes Escobedo Minerva Maldonado-Berny ME Gutierrez-Castillo Armando J.Espadas-Alvarez Manuel A.Fernandez-Parrilla Juan U.Mascotte-Cruz CP Rodríguez-Oviedo Irais E.Valenzuela-Arzeta Claudia Luna-Herrera Francisco E.lopez-Salas Jaime Santoyo-Salazar Daniel Martinez-Fong Departamento de Fisiología Biofísica y NeurocienciasCentro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Department of Pharmacology Wayne State University School of MedicineDetroitMIUSA Laboratorio de Patogénesis Molecular Carrera Médico CirujanoFacultad de Estudios Superiores IztacalaUniversidad Nacional Autónoma de MéxicoTlalnepantla de BazMéxico Red de Medicina para la Educación y Desarrollo y la Investigación Científica de Iztacala(Red MEDICI) Carrera Médico CirujanoFacultad de Estudios Superiores IztacalaUniversidad Nacional Autónoma de MéxicoTlalnepantla de BazMéxico Nanoparticle Therapy Institute AguascalientesMéxico Departamento de Biociencias e Ingeniería Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y DesarrolloInstituto Politécnico NacionalCiudad de MéxicoMéxico Escuela Nacional de Medicina y Homeopatía Instituto Politécnico NacionalCiudad de MéxicoMéxico Departamento de Fisiología Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalCiudad de MéxicoMéxico Departamento de Biología Molecular y Biotecnología Instituto de Investigaciones BiomédicasUniversidad Nacional Autónoma de MéxicoCiudad de MéxicoMéxico Departamento de Física Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Programa de Nanociencias y Nanotecnología Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico

Parkinsonism by unilateral,intranigralβ-sitosterolβ-D-glucoside administration in rats is distinguished in that theα-synuclein insult begins unilaterally but spreads bilaterally and increases in severity over time,thus replicating several clinical features of Parkinson’s disease,a typicalα-*** Nurr1 repressesα-synuclein,we evaluated whether unilateral transfected of rNurr1-V5 transgene via neurotensin-polyplex to the substantia nigra on day 30 after unilateralβ-sitosterolβ-D-glucoside lesion could affect bilateral neuropathology and sensorimotor deficits on day 30 *** study found that rNurr1-V5 expression but not that of the green fluorescent protein(the negative control)reducedβ-sitosterolβ-D-glucoside-induced ***,a bilateral increase in tyrosine hydroxylase-positive cells and arborization occurred in the substantia nigra and increased tyrosine hydroxylase-positive ramifications in the *** addition,tyrosine hydroxylase-positive cells displayed less senescence markerβ-galactosidase and more neuron-cytoskeleton markerβIII-tubulin and brain-derived neurotrophic factor.A significant decrease in activated microglia(positive to ionized calcium-binding adaptor molecule 1)and neurotoxic astrocytes(positive to glial fibrillary acidic protein and complement component 3)and increased neurotrophic astrocytes(positive to glial fibrillary acidic protein and S100 calcium-binding protein A10)also occurred in the substantia *** effects followed the bilateral reduction inα-synuclein aggregates in the nigrostriatal system,improving sensorimotor *** results show that unilateral rNurr1-V5 transgene expression in nigral dopaminergic neurons mitigates bilateral neurodegeneration(senescence and loss of neuron-cytoskeleton and tyrosine hydroxylase-positive cells),neuroinflammation(activated microglia,neurotoxic astrocytes),α-synuclein aggregation,and sensorimotor *** neurotrophic astrocytes and

关键词： A1 astrocytes A2 astrocytes gene therapy microglia motor deficits nanoparticles neurodegeneration neuroinflammation senescence α-synuclein aggregates

Simulation Tools for a Fiber-Optic Based Structural Health Monitoring System

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Transactions of Nanjing University of Aeronautics and Astronautics 2018年第2期35卷 219-225页

作者： Alfredo Güemes Antonio Fernandez-lopez Jaime García-Ramírez Maria Eugenia reyes-Perez Flor Criado Zurita Laboratory for Composites & Smart Structures Department of AeronauticsPolytechnic University of Madrid

Probability of detection(POD)graphics allow for a change from qualitative to quantitative assessment for every damage detection system,and as such it is a main request for conventional non-destructive testing(NDT)*** availability can greatly help towards the industrialization of the corresponding Structural health monitoring(SHM)*** having in mind that for SHM systems the sensors are at fixed positions,and the location of a potential damage would change its *** robust simulation tools are required to obtain the model assisted probability of detection(MAPOD)which is needed to validate the SHM *** tool may also help for the optimization of the sensor distribution,and finally will allow a probabilistic risk ***,simulation of ultrasonic waves SHM system,was a main milestone in this *** article deals with the simulation tools for a strain based SHM system,using fiber optic sensors(FOS).FOS are essentially strain/temperature sensors,either with multi-point or with distributed *** simulation tool includes the finite element model(FEM)for the original and damaged structure,and algorithms to compare the strain data at the pre-established sensors locations,and from this comparison to extract information about damage occurrence and *** study has been applied to the structure of an all-composite unmanned aircraft vehicle(UAV)now under construction,designed at Universidad Politecnica de Madrid for the inspection of electrical utilities *** sensing optical fibers were internally bonded at the fuselage and *** inspection is planned to be done with the aircraft at the test bench by imposing known *** the acquired strain data,damage occurrence may be calculated as slight deviations from the *** is a fast inspection procedure without requiring trained specialists,and it would allow for detection of hidden *** indicates that stringer par

关键词： damage detection fiber optics distributed sensing finite element models probability of detection(POD) principal component analysis (PCA) fibre bragg gratings (FBG)

Raman-IR vibrational and XRD characterization of ancient and modern mineralogy from volcanic eruption in Tenerife Island： Implication for Mars

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Geoscience Frontiers 2016年第4期7卷 673-681页

作者： E.A.Lalla G.lopez-reyes A.Sansano A.Sanz-Arranz j.Martinez-Frias J.Medina F.Rull-Perez department of physics faculty of science university of la laguna san cristobal de la laguna santa cruz de tenerifeCP 38206 spain Unidad Asociada al Centro de Astrobiologfa CSIC-INTA Associated to the NASA Astrobiology Center-Instituto Nacional de Tecnica Aeroespacial Torrejon de ArdozCP 28850MadridSpain Department of Earth Dynamics and Observation lnstituto de Ceociencias IGEO-(CSIC-UCM)Facultad de Ciencias Geologicas.Ciudad UniversitariaCP 28040MadridSpain

A detailed vibrational Raman-lR spectroscopic and diffractional analyses have been performed on basalts from two locations from Tenerife Island：（1） the Arenas Negras volcano which belongs to the historical eruption not showing visible alteration and （2） Pillow Lavas zone from Anaga Massif which shows a clearly fluid-rock interaction caused by submarine alteration. These places have been extensively studied due to its similarity with the surface of Mars. The analysis is based on the mineral detection of selected samples by a Micro-Raman study of the materials. The complementary techniques have confirmed the mineralogy detected by the Raman measurement. The results show a volcanic environment behavior with primary phases like olivine, pyroxene, and feldsparJplagioclase. Moreover, the presence of accessory minerals or secondary mineralization like phosphate, iron oxides, zeolite or carbonates shows the alteration processes on each outcrop. The variation in the crystallinity and amorphous phases is related to fluid-rock interaction caused by hydrothermal episodes and external weathering processes, which shows several analogies with the ancient volcanic activity from Mars.

关键词： Mars Volcanoes Terrestrial analog Raman spectroscopy Tenerife Island Mineralogy

Extreme Hyperferritinemia in a Filipino Male Patient with Adult Onset Still’s Disease: A Case Report

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Open Journal of Rheumatology and Autoimmune Diseases 2022年第4期12卷 119-127页

作者： Denise Joy Emmanuelle lopez Karla Sofia reyes Aileen U. Agbanlog Section of Rheumatology Department of Medicine St. Luke’s Medical Center Quezon City Metro Manila Philippines

Background: Elevated serum ferritin is more commonly due to reactive causes such as infection, hepatic disorders, rheumatologic conditions, and malignancy than true iron overload. Extreme hyperferritinemia (>10,000 ng/mL), on the other hand, should prompt consideration of rare conditions such as adult-onset Still’s disease (AOSD) or hemophagocytic lymphohistiocytosis. This paper aims to present the case of the highest reported extreme hyperferritinemia (actual level 256,000 ng/mL) in a patient eventually diagnosed with adult onset Still’s Disease (AOSD). Case Presentation: A 55-year-old male, Filipino, was admitted due to acute onset fever and shortness of breath. He was initially assessed to have community-acquired pneumonia and a suspect for coronavirus disease 2019 (COVID-19), hence inflammatory markers were requested. Ferritin was notably elevated at 44,255 ng/mL. He eventually tested negative for COVID-19 RT-PCR. He was investigated for other causes of markedly elevated ferritin levels. His complete blood count (CBC) only showed leukocytosis with no peripheral blasts, iron level and liver function tests were normal, HIV immunoassay was negative, ANA was 1:80 speckled with normal complement level, rheumatoid factor negative, and positron emission tomography (PET) scan revealed presence of lymphadenopathies and did not show solid tumors. He was treated for urinary tract infection and pneumonia but still had intermittent fever and increasing ferritin trend, with the highest documented level at 256,000 ng/mL. Fulfilling the Yamaguchi criteria, he was managed as a case of severe AOSD and received tocilizumab. He had lysis of fever and decreasing trend of ferritin levels thereafter, with ferritin level of 34,184 ng/mL three weeks after tocilizumab infusion. He was discharged and improved with prednisone and methotrexate as home medications. Conclusion: To our knowledge, the highest level of extreme hyperferritinemia recorded in literature as of 2016 is 143,931 ng/mL,

关键词： Still’s Disease Hyperferritinemia Autoimmune Tocilizumab Case Report

Identification and heterologous expression of the globomycin biosynthetic gene cluster

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Synthetic and Systems Biotechnology 2023年第2期8卷 206-212页

作者： Daniel Oves-Costales Tetiana Gren Eva Baggesgaard Sterndorff Jesús Martín Francisco Javier Ortiz-lopez Tue S.Jørgensen Xinglin Jiang Fernando Roman-Hurtado Fernando reyes Olga Genilloud Tilmann Weber Fundacion MEDINA Centro de Excelencia en Investigaci´on de Medicamentos Innovadores en AndalucíaAvda del Conocimiento 3418016ArmillaGranadaSpain The Novo Nordisk Foundation Center for Biosustainability Technical University of DenmarkKemitorvetbuilding 2202800 Kgs.LyngbyDenmark

Globomycin is a cyclic lipodepsipeptide originally isolated from several Streptomyces species which displays strong and selective antibacterial activity against Gram-negative *** mode of action is based on the competitive inhibition of the lipoprotein signal peptidase II(LspA),which is absent in eukaryotes and considered an attractive target for the development of new *** its interesting biological properties,the gene cluster encoding its biosynthesis has not yet been *** this study we employed a genome-mining approach in the globomycin-producing Streptomyces ***-278952 to identify a candidate gene cluster responsible for its biosynthesis.A null mutant was constructed using CRISPR base editing where production was abolished,strongly suggesting its involvement in the *** putative gene cluster was then cloned and heterologously expressed in Streptomyces albus J1074 and Streptomyces coelicolor M1146,therefore unambigu-ously linking globomycin and its biosynthetic gene *** work paves the way for the biosynthesis of new globomycin derivatives with improved pharmacological properties.

关键词： Globomycin Signal peptidase II Cyclic lipodepsipeptide Genome mining CRISPR-cBEST Heterologous expression

Mixed Wastes Vitrification by Transferred Plasma

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Plasma Science and Technology 2007年第6期9卷 721-724页

作者： J.TAPIA-FABELA M.PACHECO-PACHECO J.PACHECO-SOTELO C.TORRES-reyes R.VALDIVIA-BARRIENTOS J.BENITEZ-READ R.lopez-CALLEJAS F.RAMOS-FLORES S.BOSHLE G.ZISSIS Technological National Plasma and Energy Conversion Laboratory 118 Narbonne AveToulousecedex 4431062France

Thermal plasma technology provides a stable and long term treatment of mixed wastes through vitrification processes. In this work, a transferred plasma system was realized to vitrify mixed wastes, taking advantage of its high power density, enthalpy and chemical reactivity as well as its rapid quenching and high operation temperatures. To characterize the plasma discharge, a temperature diagnostic is realized by means of optical emission spectroscopy （OES）. To typify the morphological structure of the wastes samples~ scan- ning electron microscopy （SEM）, and X-ray diffraction （XRD） techniques were applied before and after the plasma treatment.

关键词： mixed wastes vitrification transferred plasma plasma characterisation

Association between Aspergillus flavus Colonization and Aflatoxins Production in Immature Grains of Maize Genotypes

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Journal of Food Science and Engineering 2013年第12期3卷 688-698页

作者： Maria Carolina de Luna-lopez Arturo Gerardo Valdivia-Flores Fernando Jaramillo-Juarez Jose Luis reyes Raul Ortiz-Martinez Teodulo Quezada-Tristfin Agricultural Sciences Centre Mycotoxicology Aguascalientes Autonomous University Aguascalientes 20131 Mexico Basic Sciences Centre Toxicology Aguascalientes Autonomous University Aguascalientes 20131 M~xico Department of Physiology and Biophysics Toxicology Center for Research and Advanced Studies-l. P. N. Mdxico D. F. 07360Mexico

Aspergillus flavus maize colonization leads to crop contamination by toxic secondary metabolites and carcinogens called aflatoxins （AF）; it has negative effects in public health and has caused economic losses in agricultural activities. Eleven genotypes of immature maize grain frequently used in Mexico were inoculated in vitro with two indigenous toxigenic strains ofA. flavus. The size of inoculum, temperature, humidity and presence of other phytopathogens were assessed. Genotypes Popcorn, C-526, Garst 8366, As910 and 30G40 showed resistance to rating of fungal colonization （FC） and AF accumulation, while 3002W, 30R39, Creole, C-922, HV313 and P3028W genotypes were less resistant. AFB~ had the highest concentrations （26.1 mg/kg ± 14.7 mg/kg）, while AFB2, AFG1 and AFG2 showed only residual concentrations 1.6, 2.0 and 4.0 ~tg/kg, respectively. Concerning FC and AF, there were significant differences （P 〈 0.01） between strains and genotype. Both strains showed significant association （P 〈 0.01） between FC and the concentrations of AFB1 and AFB2 （R2： 99.5% and 93.2%; 87.2% and 73.2%, respectively）. Results suggest that the level of resistance to fungus infection and AF accumulation is related to maize genotype. It emphasizes the relevance of developing A. flavus resistant maize genotypes as an alternative to control contamination in foodstuff intended for human and animal consumption.

关键词： AF Aspergillusflavus immature maize grain resistance Mexico.

Polymerization Reactor Monitoring by In-line Raman Spectrometry

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材料科学与工程（中英文A版） 2017年第6期7卷 303-310页

作者： Ramiro Infante Martinez Andre Olivos Ramirez Ricardo lopez Gonzalez Ramon Diaz de Leon Luis Villarreal Cardenas Esther Trevino Martinez Alejandro Diaz Elizondo Beatriz reyes Vielma Procesos de polimerizacion Centro de Investigacion de Qulmica Aplicada Saltillo Coahuila 25294 Mexico