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Automatic Computer Analysis of Digital Images of Triple-Antibody-Stained Prostate Biopsies

Open Journal of Urology 2021年第1期11卷 17-29页

作者： Erik Wilander Manuel de la torre Ursula Wilhelmsson Sö ren Nygren department of Pathology County Hospital G&#228 vle Sweden MM18 Medical AB Uppsala Sweden

Background: Worldwide, prostatic adenocarcinoma is the most common tumour type among men. Aim: The aim of the present investigation was to develop a computer program to identify normal prostate biopsies and distinguish them from biopsies showing premalignant alterations (LGPIN, HGPIN) and adenocarcinoma. Method: Prostate biopsies (n = 2094) taken from 191 consecutive men during 2016 were stained with triple immunehistochemisty (antibodies to AMACRA, p63 and CK 5). Digital images of the biopsies were obtained with a scanning microscope and used to develop an automatic computer program (CelldaTM), intended to identify the morphological alterations. Visual microscopic finding was used as a reference. Result: Of the 191 men, 121 (63.4%) were diagnosed as having prostate adenocarcinoma and 70 (36.6%) as having no malignancy on the basis of the visual microscopy. In comparison, computer analysis identified 134 (70.2%) men with malignant disease and 57 (29.8%) with non-malignant disease after exclusion of artifacts, which constituted 10.4% of areas (indicated as malignant disease). Discrepant results were recorded in 15 (7.9%) men, and in 14 of these cases, HGPIN and areas suggestive of early invasion were common. Thus, it was uncertain whether these cases should be regarded as malignant or not. The agreement between the visual examination and the computer analysis was 92.1% (kappa value 0.823, sensitivity 99.2 and specificity was 0.80). Conclusion: It seems that computer analysis could serve as an adjunct to simplify and shorten the diagnostic procedure, first of all by ensuring that normal prostate biopsies are sorted out from those sent for visual microscopic evaluation.

关键词： Prostate Adenocarcinoma LGPIN HGPIN Antibody Computer Digital Images Automatic Analysis AMACR P504S Microscopy Scanning

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Antimicrobial Resistance Genes in Animal Manure, Manure-Amended and Nonanthropogenically Impacted Soils in Spain

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Advances in Bioscience and Biotechnology 2018年第9期9卷 469-480页

作者： Fernando Esperón Carlos Sacristán Matilde Carballo Ana de la torre Grupo de Epidemiología y Sanidad Ambiental Centro de Investigación en Sanidad Animal (INIA-CISA) Valdeolmos Spain

Environmental dissemination of antimicrobial resistance genes may occur through agricultural residues, such as animal manure. We studied the resistome of 16 pool samples of animal manure (pig slurry [n = 8] and poultry manure [n = 8]), and 16 soil samples (manure-amended [n = 8] and nonmanure-amended [n = 8]). All samples were collected in central Spain. detection was based on 18 selected antimicrobial resistance genes (ARGs). The most commonly detected genes in animal manure were sul1 (16/16), sul2 (16/16), tet(A) (16/16), aadA (16/16), tet(B) (15/16), and str (15/16). Genes blaTEM (7/8), mecA (6/8), vanA (5/8) and qnrB (4/8) were more frequently detected in chicken manure, whereas pig slurry samples presented higher levels of tet(C) (8/8) and tet(M) (8/8). Out of the four genes selected for their clinical relevance, three—blaCTX-M, vanA, and mecA—were detected in animal manure. The blaCTX-M (1/8) and vanA (5/8) genes were only identified in chicken manure. To our knowledge, this is the first report of direct detection of mecA gene in poultry manure and pig slurry. Eleven out of 18 ARGs were detected in amended soil, while only genes sul2 (3/8) and str (2/8) were found in nonanthropogenically impacted soils (NAIS), supporting the hypothesis that ARGs may serve as indicators of “anthropogenic impact” on the environment.

关键词： Antibiotic Resistance blaCTX-M mecA Pig Slurry vanA

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Environmental Assessment of Tetracycline’s Residues detected in Pig Slurry and Poultry Manure

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Journal of Environmental Protection 2016年第1期7卷 82-92页

作者： Matilde Carballo Sonia Aguayo Miguel González Fernando Esperon Ana de la torre Research Centre in Animal Health CISA-INIA Madrid Spain Group of Water Pollution ISC III Madrid Spain

The widespread use of antimicrobials in animal husbandry has led to an increase in concentrations of antibiotics in animal faeces. Furthermore, the spreading of this waste as fertilizer on agricultural land indirectly introduces antimicrobial residues into the environment. This study examined samples of pig slurry and poultry manure for three tetracyclines—oxytetracycline (OTC), chlortetracycline (CTC) and doxycycline (DOC) and conducted an environmental evaluation of soils. The samples were taken from manure heaps on eight broiler chicken farms and from slurry ponds on eight pig farms. The tetracycline analysis was conducted using high-performance liquid chromatography combined with tandem mass spectrometry (HPLC/MS). The risk assessment was carried out on the basis of the estimated PECsoil value and the ecotoxicological information available for terrestrial organisms. TC concentrations were between >0.01 and 1.38 mg/kg. OCT was detected in 37% of the poultry manure and in 20% of the pig slurry samples. DOC was present in 50% of pig slurry samples with an average concentration of 1.2 mg/kg, while CTC was only found in one slurry sample at a concentration of 0.56 mg/kg. Compared with published values for these two farm animals from other countries, the TC concentrations obtained in this study were low. The environmental importance of the data generated was also discussed.

关键词： Tetracyclines Pig Slurry Poultry Manure Risk Evaluation

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Study by Fourier Transform Infrared Spectroscopy of the Avocado Oils of the Varieties Hass, Criollo and Fuerte

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材料科学与工程（中英文版） 2010年第5期4卷 61-64页

作者： Jose alberto Ariza-Ortega Maria Guadalupe Mendez-Ramos Joel Diaz-Reyes Rafil Jacobo delgado-Macuil Rafil Rend Robles-de-la-torre Centro de Investigaci6n en Biotecnologia Aplicada del Instituto Polit~cnico Nacional Ex-Hacienda de San Juan Molino. Km. 1.5.Tepetitla Tlaxcala 90700 Mexico

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Risk factors for suicidal behaviour in late-life depression:A systematic review

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World Journal of Psychiatry 2022年第1期12卷 187-203页

作者： Veronica Fernandez-Rodrigues Yolanda Sanchez-Carro Luisa Natalia lagunas laura Alejandra Rico-Uribe Andres Pemau Patricia Diaz-Carracedo Marina Diaz-Marsa Gonzalo Hervas Alejandro de la torre-Luque department of Psychology Universidad Complutense de MadridMadrid 28223Spain department of Psychiatry Universidad Autonoma de MadridMadrid 28046Spain Centre for Biomedical Research in Mental Health(CIBERSAM) Madrid 28029Spain department of Legal Medicine Psychiatry and PathologyUniversidad Complutense de MadridMadrid 28046Spain of Psychology La Rioja International UniversityLogrono 26006Spain Institute of Psychiatry and Mental Health San Carlos Clinical HospitalMadrid 28040Spain

BACKGROUND Suicide is a leading cause of preventable death worldwide,with its peak of maximum incidence in later *** often puts an individual at higher risk for suicidal *** turn,depression deserves particular interest in old age due to its high prevalence and dramatic impact on health and *** To gather integrated evidence on the potential risk factors for suicide behaviour development in depressive older adults,and to examine the effects of depression treatment to tackle suicide behaviour in this *** A systematic review of empirical studies,published from 2000 onwards,was *** behaviour was addressed considering its varying forms(i.e.,wish to die,ideation,attempt,and completed suicide).RESULTS Thirty-five papers were selected for review,comprising both clinical and epidemiological *** of studies focused on suicidal ideation(60%).The studies consistently pointed out that the risk was related to depressive episode severity,psychiatric comorbidity(anxiety or substance use disorders),poorer health status,and loss of *** social support and loneliness were also associated with suicide behaviour in depressive older ***,the intervention studies showed that suicidal behaviour was a robust predictor of depression treatment *** in suicidal ideation were moderated by reductions in risk factors for suicide *** To sum up,common and age-specific risk factors seem to be involved in suicide development in depressive older adults.A major effort should be made to tackle this serious public health concern so as to promote older people to age healthily and well.

关键词： late-life depression Suicide behaviour Disability Chronic disease Loneliness

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Anti-money laundering in Banks:Towards a Model for Training Evaluation

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Management Studies 2022年第2期10卷 115-124页

作者： Michele Samuele Borgia Maura la torre University G.D’Annunzio PescaraItaly

This paper aims to propose an evaluation model specific for anti-money laundering training in *** model was structured taking from some of the most popular training evaluation models proposed in the literature the evaluation levels considered suitable for evaluating anti-money laundering training *** additional evaluation levels are included,which assess possible exposure of training activity to the risks associated with knowledge *** originality of the proposed model lies in the fact that,to the best of the authors’knowledge,to date,there is still no specific model for evaluating anti-money laundering training in *** the limitations of this study is the fact that,for the moment,the proposed model is a in a purely theoretical formulation.

关键词： training evaluation knowledge risks model banks

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Effect of the thin cell layer technique in the induction of somatic embryos in Pinus patula Schl. et Cham

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Journal of Forestry Research 2019年第4期30卷 1535-1539页

作者： Marco A.Ramirez-Mosqueda Lourdes G.Iglesias-Andreu Arturo A.Armas-Silva Esmeralda J.Cruz-Gutierrez Jose F.de la torre-Sanchez Otto R.Leyva-Ovalle Carlos M.Galan-Paez Instituto de Biotecnologfa y Ecologfa Aplicada(INBIOTECA) Universidad Veracruzana.Av.de las Culturas Veracruzanas No.101Campus para la Culturalas Artes y el DeporteCol.Emiliano ZapataC.P.91090 XalapaVeracruzMexico Centro Nacional de Recursos Geneticos-INIFAP Boulevard de la Biodiversidad No.400 Rancho las CrucesC.P.47600 Tepatitlan de MorelosJaliscoMexico Facultad de Ciencias Bioldgicas y Agropecuarias Region Cordoba-Orizaba Universidad VeracruzanaC.P.94945 Amatlan de los ReyesVeracruzMexico

Pinus patula is a species commonly used for reforestation in ***,efficient methods for the mass production seedlings are *** particularly by somatic embryogenesis provides an option for the rapid multiplication of high-quality,genetically improved *** study induces somatic embryogenesis in this species using the thin cell layer(TCL)*** sources of explants(complete immature embryos;lTCL segments from immature embryos)were *** efficiency of TCL from longitudinal sections[lTCL]and transverse[tTCL]was *** results show using thin cell layers from immature embryos cultivated in 16 light/8 dark hours achieves induction of somatic embryos.A higher percentage of embryogenic callus was obtained when tTCL segments were used as an explant *** results produced somatic embryos from tTCL segments of an immature embryo without germinating the seed,making the process more time *** addition,this technique can be used to generate somatic embryogenesis in forest species that have low germination rates.

关键词： Pinus patula Somatic embryos Micropropagation Thin cell layer Maturation

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Analyzing the genomic and transcriptomic architecture of milk traits in Murciano-Granadina goats

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Journal of Animal Science and Biotechnology 2020年第3期11卷 649-666页

作者： Dailu Guan Vincenzo landi María Gracia Luigi-Sierra Juan Vicente delgado Xavier Such Anna Castelló Betlem Cabrera Emilio Mármol-Sánchez Javier Fernández-Alvarez JoséLuis Ruiz de la torre Casañas Amparo Martínez Jordi Jordana Marcel Amills Centre for Research in Agricultural Genomics(CRAG) CSIC-IRTA-UAB-UBUniversitat Autònoma de Barcelona08193 BellaterraSpain departamento de Genética Universidad de Córdoba14071 CórdobaSpain departament de Ciència Animal i dels Aliments Facultat de VeterinàriaUniversitat Autònoma de Barcelona08193 BellaterraSpain Asociación Nacional de Criadores de Caprino de Raza Murciano-Granadina(CAPRIGRAN) 18340 GranadaSpain Servei de Granges i Camps Experimentals Universitat Autònoma de Barcelona08193 BellaterraSpain

Background:In this study,we aimed to investigate the molecular basis of lactation as well as to identify the genetic factors that influence milk yield and composition in *** achieve these two goals,we have analyzed how the mRNA profile of the mammary gland changes in seven Murciano-Granadina goats at each of three different time points,i.e.78 d(T1,early lactation),216 d(T2,late lactation)and 285 d(T3,dry period)after ***,we have performed a genome-wide association study(GWAS)for seven dairy traits recorded in the 1st lactation of 822 Murciano-Granadina ***:The expression profiles of the mammary gland in the early(T1)and late(T2)lactation were quite similar(42 differentially expressed genes),while strong transcriptomic differences(more than one thousand differentially expressed genes)were observed between the lactating(T1/T2)and non-lactating(T3)mammary glands.A large number of differentially expressed genes were involved in pathways related with the biosynthesis of amino acids,cholesterol,triglycerides and steroids as well as with glycerophospholipid metabolism,adipocytokine signaling,lipid binding,regulation of ion transmembrane transport,calcium ion binding,metalloendopeptidase activity and complement and coagulation *** regard to the second goal of the study,the performance of the GWAS allowed us to detect 24 quantitative trait loci(QTLs),including three genome-wide significant associations:QTL1(chromosome 2,130.72-131.01 Mb)for lactose percentage,QTL6(chromosome 6,78.90-93.48 Mb)for protein percentage and QTL17(chromosome 17,11.20 Mb)for both protein and dry matter ***,QTL6 shows positional coincidence with the casein genes,which encode 80%of milk ***:The abrogation of lactation involves dramatic changes in the expression of genes participating in a broad array of physiological processes such as protein,lipid and carbohydrate metabolism,calcium homeostasis,cell death and tissue remo

关键词： Casein genes Dairy traits GWAS lactation QTLs RNA-Seq

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Use of Oat Bran in Bread: Fiber and Oil Enrichment and Technological Performance

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Food and Nutrition Sciences 2011年第6期2卷 553-559页

作者： Sofia Beccerica María A. de la torre Hugo D. Sanchez Carlos A. Osella Instituto de Tecnología de Alimentos Facultad de Ingeniería Química Universidad Nacional del Litoral Santa Fe Argentina.

The effect of different amounts of oat bran on breadmaking was studied using also additives in the formulation. The aim of this experimental work was to evaluate the technological performance of oat bran in bread and the nutritional improvement resulting from the increased content of fiber and oil. Data were analyzed by ANOVA and the results were compared by Duncan’s test at a significance level of 0.05. With the addition of oat bran, the specific volume varied in such a way that the greater the amount of bran in the mixture, the lower the specific volume of bread. The addition of the proposed additives, however, helped significantly increase the volume. Besides, a change in the fatty acid profile, with a higher content in unsaturated fatty acids, as well as larger amounts of dietary fiber, was observed.

关键词： Bread Dietary Fiber Nutrition Oat Bran Profile of Fatty Acids

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Comparison of a Soil Vulnerability Map for Tetracycline Soil Contamination at a Global and Local Scale

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Journal of Environmental Protection 2013年第6期4卷 595-601页

作者： Ana de la torre Irene Iglesias Luis Miguel Camunas Maria Jesus Munoz Matilde Carballo Research Centre in Animal Health CISA-INIACtra Algete-El Casar s/nMadridSpain

Recently, de la torre[1] provided an approach for assessing the vulnerability of European soils for tetracyclines, fluoroquinolones and sulfamides contamination using a spatial risk assessment. It allowed identifying areas where vulnerable soil more occur, providing useful information for policies designed to reduce contamination. In the current study, this approach was applied to a local level, the autonomous region of Castile and León (C&L), located in the north-west part of Spain. High accurate and reliable source data were employed to generate a soil vulnerability map for tetracyclines in the study area, attempting to improve the release and consequence risk prediction. More specifically, pig density, temperature and soil use risk estimators were improved using data from national or local databases. Result comparison between the European and the present approach showed a good agreement demonstrating the utility of the European vulnerability map to be employed not only at global level but also for deciding how to allocate limited resources on national or subnational environmental surveillance programs of antibiotics. The model application at local level using more accurate data from national or local sources, afforded a better understanding of the spatial distribution of the risk, mainly associated with the higher accuracy of the national land use database SIOSE. It could offer a useful tool for local management of the risk, such as the management of animal manures fertilization on soil.

关键词： Tetracyclines Soil Contamination Vulnerability Map Europe GIS

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