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Time till death affects spider mobility and web-building behavior during web construction in an orb-web spider

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Current Zoology 2016年第2期62卷 123-130页

作者： Mylene ANOTAUX Camille TOSCANI Raymond LEBORGNE Nicolas CHALINE alain pasquet Faculte des Sciences et Technologies Universite de Lorraine 54506 Vandoeuvre Nancy France Ecole nationale veterinaire d'Alfort Universite Paris-Est UMR 7179 CNRS MNHN Maisons-Alfort F-94704 France Laboratoire d'Ethologie Experimentale et Comparee EA 4443 Universite Paris 13 99 avenue J.B. Clement 93430 Villetaneuse France Faculte des Sciences et Technologies Universite de Lorraine CNRS UR AFPA. B.P. 239 54506 Vandoeuvre Nancy France

It is well known that age influences organism mobility. This was demonstrated in vertebrates （such as mammals and birds） but has been less studied in invertebrates with the exception of Drosophila and the nematode Caenorhabditis elegans. Here we studied the influence of age on the mobility of the orb-weaving spider Zygiella x-notata during web construction. The orb-web is a good model because it has a characteristic geometrical structure and video tracking can be used to easily follow the spider＇s movements during web building. We investigated the influence of age （specifically chronological age, life span, and time till death） on different parameters of spider mobility during the construction of the capture spiral （distance traveled, duration of construction, spider velocity, spider movement, and spider inactivity） with a generalized linear model （GLM） procedure adjusted for the spider mass. The re- sults showed that neither chronological age, nor life span affected the mobility parameters. However, when the time till death decreased, there was a decrease in the distance traveled, the duration of the construction of the capture spiral, and the spider movement. The spider velocity and the time of inactivity were not affected. These results could be correlated with a decrease in the length of the silky thread deposited for the construction of the capture spiral. Spiders with a shorter time till death built smaller web using less silk. Thus, our study suggests strongly that time till death affects spider mobility during web construction but not the chronological age and thus may be a good indicator of senescence.

关键词： ageing behaviors orb-web construction spider mobility Zygiella x-notata.

Minigene Analysis of the c.743 + 40A >G Intronic Variant in the <i>CFTR</i>Gene

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Health 2014年第12期6卷 1352-1360页

作者： Ayman El-Seedy Raed Farhat Marie-Claude pasquet alain Kitzis Véronique Ladeveze Génétique Moléculaire de Maladies Rares P&ocirc le Biologie Santé Université de Poitiers Poitiers France

Background: Since the identification of the cystic fibrosis transmembrane regulator (CFTR) gene in 1989, many polymorphisms have been identified in cystic fibrosis (CF) or CFTR-related disorders (CFTR-RDs) patients and still remain to be characterized at the molecular level. These polymorphisms are difficult to classify as pathogenic or non-disease causing because the polymorphisms are either located in the coding region, but are synonymous, or are found in the intronic regions. Here we investigated the potential impact of the c.743 + 40A > G polymorphism within CFTR intron 6 on the alternative splicing. Indeed, this variant has been observed frequently in our examined patients. Moreover, a family carrying this variant exhibited CFTR-RD phenotype. Methods: By denaturing high pressure liquid phase chromatography (DHPLC) and sequencing, thirty of 293 subjects French origin carried the c.743 + 40A > G variant. Of these, 16 patients were affected by CF or CFTR-RD. Wild-type sequences and mutant CFTR intron 6 and its boundaries were inserted into the pTBNdeI hybride minigene and expressed in three different cell lines. After RT-PCR analysis of mRNA using specific primers, sequences of the minigene transcripts were obtained. Results: No aberrant splicing was detected with minigene carrying c.743 + 40A > G variant in all transfected cell lines. However, an alternative splicing in the positive control was detected with a minigene carrying the c.1392G > T + 1G > T mutation: 5 nucleotides were deleted from mRNA sequences, indicating that used cell lines are appropriate for studying the splicing. Conclusion: Transient transfections of a minigene containing the c.743 + 40A > G polymorphism showed no splicing errors, and thus this intronic alteration was finally classified as non-pathogenic. As it is always associated with c.2562T > G and c.4389G > A, or TG12-7T poly-morphisms, further experiments are needed to determine the role of these complex alleles in disease pathogenesis.

关键词： Cystic Fibrosis Polymorphism Complex Allele Hybrid Minigene Splicing

凝血酶受体活化与血小板膜糖蛋白Ⅰ bα的异位分布机制

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中华血液学杂志 2005年第3期26卷 152-156页

作者：韩悦 JM pasquet A Nurden 王兆钺阮长耿苏州大学附属第一医院江苏省血液研究所215006 法国国家科研中心

目的　检测凝血酶受体活化过程中血小板膜糖蛋白 (GP)Ⅰbα的动态分布及其细胞骨架蛋白的改变,揭示GPⅠbα的异位分布机制与凝血酶受体在血小板信号传递中的作用。方法　以凝血酶受体活化肽 (thrombinreceptoractivatingpeptide,TRAP)... 详细信息

目的　检测凝血酶受体活化过程中血小板膜糖蛋白 (GP)Ⅰbα的动态分布及其细胞骨架蛋白的改变,揭示GPⅠbα的异位分布机制与凝血酶受体在血小板信号传递中的作用。方法　以凝血酶受体活化肽 (thrombinreceptoractivatingpeptide,TRAP)诱导血小板活化,应用流式细胞术比较刺激前后血小板膜表面GPⅠbα及P 选择素的表达,并通过转移电泳及免疫沉淀技术,分析细胞骨架中GPⅠbα、肌动蛋白、肌球蛋白的变化。观测细胞松弛素 (Cytochalasin) D与腺苷三磷酸双磷酸酶(Apyrase)Ⅶ对血小板活化过程的影响。结果　TRAP作用后促使血小板活化,流式细胞术检测结果显示其P 选择素水平显著升高,而GPⅠbα先呈现进行性减少,而后逐渐回升的可逆性变化。抑制剂作用后这一过程受到不同程度影响,其中细胞松弛素D能阻滞GPⅠbα的内移, ApyraseⅦ对活化过程中GPⅠbα的逆转没有影响,只对它的恢复起作用,促使GPⅠbα迅速返回细胞表面。细胞骨架蛋白分析结果也表明肌动蛋白、肌球蛋白与GPⅠbα受刺激后呈先增加后减少的动态变化趋势。细胞松弛素D能阻滞GPⅠbα、肌动蛋白与肌球蛋白向骨架中心的转位, ApyraseⅦ则能加快骨架蛋白在骨架中心的消散。免疫印迹证实GPⅠbα的这种可逆性变化与血小板膜骨架紧密相关。

关键词： GP 凝血酶受体血小板膜糖蛋白血小板活化异位细胞骨架蛋白肌动蛋白细胞松弛素腺苷三磷酸双磷酸酶肌球蛋白

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蛋白酶活化受体1与4在血小板活化中的作用机制

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中国实验血液学杂志 2003年第5期11卷 495-498页

作者：韩悦 Jean-max pasquet Alan Nurden 阮长耿苏州大学附属第一医院江苏省血液研究所苏州215006 UMR 5533 CNRSHopital CardiologiquePessac 33604France

为了比较蛋白酶活化受体 1和 4(PAR1,PAR4)合成肽对血小板膜表面糖蛋白GPIbα表达及细胞骨架的影响 ,揭示蛋白酶活化受体在血小板信号传递中的作用 ,选择 2 5μmol/LPAR1与 2 50 μmol/LPAR4两类合成肽分别在不同时间段 (0 -60分钟 )活... 详细信息

为了比较蛋白酶活化受体 1和 4(PAR1,PAR4)合成肽对血小板膜表面糖蛋白GPIbα表达及细胞骨架的影响 ,揭示蛋白酶活化受体在血小板信号传递中的作用 ,选择 2 5μmol/LPAR1与 2 50 μmol/LPAR4两类合成肽分别在不同时间段 (0 -60分钟 )活化血小板 ,应用流式细胞仪测定血小板膜糖蛋白GPIbα及P 选择蛋白的表达 ,并结合SDS PAGE与转移电泳技术比较血小板活化前后细胞骨架GPIbα、肌动蛋白、肌球蛋白的变化 ,同时对膜骨架成分进行GPIbα免疫沉淀分析。结果显示 ,PAR 1与PAR4两类合成肽均可促使血小板活化 ,其P 选择蛋白水平显著升高 ,GPIbα表达进行性减少又出现逐渐回升的可逆性变化 ,各时间段引起的GPIbα改变在两者之间都具有显著差异 (P <0 .0 5) ,其中PAR1首先发生作用 ,但PAR4维持时间更长。细胞骨架蛋白分析显示肌动蛋白与肌球蛋白协同GPIbα呈现先增加后减少的动态变化。免疫印迹也表明与GPIbα相连的肌动蛋白及肌球蛋白出现可逆性改变。结论 :PAR1与PAR4在血小板信号传递过程中发挥了重要作用 ,它们能各自独立地介导血小板活化 ,并导致GPIbα逆转 ,这与细胞骨架的积极参与相关。PAR1起效较快。

关键词：血小板活化蛋白酶活化受体糖蛋白Ibα 细胞骨架

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A New Strategy for the Synthesis of Monomethylhydrazine Using the Raschig Process

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Advances in Chemical Engineering and Science 2021年第3期11卷 228-238页

作者： Duc Minh Le Anne-Julie Bougrine Olivier Duclos Véronique pasquet Henri Delalu Laboratoire Hydrazines et Composés Energétiques Polyazotés Université Claude Bernard Lyon 1 Villeurbanne France UCBL/CNRS/CNES/ArianeGroup Batiment Raulin Villeurbanne France

A new strategy for the development of monomethylhydrazine (MMH) using the Raschig process is discussed in this publication. The determination of optimal conditions for the synthesis of MMH required the development of a kinetic model. In order to validate the results of the calculation, a device for synthesis under pressure (13 bar) by direct injection of the stoichiometric monochloramine, synthesized using microreactor technology, into monomethylamine (MMA) was developed. The experimental data, in accordance with the theoretical results of the kinetic model, make it possible to establish a new process for the synthesis of monomethylhydrazine.

关键词： Synthesis Raschig Monomethylhydrazine Kinetic Model

A New Strategy for the Preparation of N-Aminopiperidine Using Hydroxylamine-O-Sulfonic Acid: Synthesis, Kinetic Modelling, Phase Equilibria, Extraction and Processes

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Advances in Chemical Engineering and Science 2013年第2期3卷 157-163页

作者： E. Labarthe A. J. Bougrine Véronique pasquet H. Delalu Laboratoire Hydrazines et Composés Energétiques Polyazotés UMR CNRS-CNES-SME-Groupe Safran Université Claude Bernard Lyon 1 Villeurbanne France

A new strategy for the synthesis of N-aminopiperidine (NAPP) was developed using hydroxylamine-O-sulfonic acid (HOSA). A systematic study of NAPP formation and degradation reactions was carried out in diluted medium, in order to identify products and to establish a kinetic modelling. Principal parameters have been defined, in particular, that obtaining high yields (>90%) requires non stoichiometric conditions. The extraction and purification processes were also studied. NAPP isolation and piperidine recycling were optimized after the establishment of the various solid-liquid-liquid and liquid-vapour implied phase diagrams. At least, a calorimetric study of solvatation and reaction enthalpies was undertaken in order to estimate reactor heating temperature in the case of anhydrous synthesis. The combination of our kinetic, thermodynamic and calorimetric data allows the establishment of two process schemes: one using pure piperidine, the other, a 66 w% titrating azeotropic solution in piperidine.

关键词： Natural Asset Financial Value Neural Network

INTESPACE试验中心为阿里安娜-V型火箭而进行的改造

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航天器环境工程 2002年第1期19卷 42-48页

作者： J.L.Marcé J.C.pasquet 闫德葵冯伟泉 INTESPACE FRANCE 北京卫星环境工程研究所北京100029

文章介绍了法国宇航环境工程试验中心(INTESPACE)为满足阿里安娜-V 的需要所准备进行的一系列改造。这些工作主要有:增建一个适合大型容器用的空气锁,保持厅内连续的10万级洁净度,建立一个紧凑试验区域,建造一面强效吸波墙,扩展振动设... 详细信息

文章介绍了法国宇航环境工程试验中心(INTESPACE)为满足阿里安娜-V 的需要所准备进行的一系列改造。这些工作主要有:增建一个适合大型容器用的空气锁,保持厅内连续的10万级洁净度,建立一个紧凑试验区域,建造一面强效吸波墙,扩展振动设备能力以及改造原热真空设备。这些工作计划于2001年初全部完成。改造工作的完成将保证 INTESPACE 在独立的欧洲试验中心中继续处于领先地位。

关键词：环境试验设备改造

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MDCT和MR对主动脉返流严重度和机制的综合评价

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国际医学放射学杂志 2010年第2期 198-199页

作者： *** V.Kersten A.C.Pouleur J.B.le Polain de Waroux D.Vancraeynest A.pasquet 范丽 Cardiology Division Cliniques Universitaires St.Luc UCLAv Hippocrate 10/28061200 Woluwe St.LambertBelgium

近来研究表明心脏MR和MDCT通过计算返流口解剖面积的方法可对主动脉返流(AR)进行定量评价,但此种测量并未与AR的定量评价如邻近等值面积区(PISA)经胸壁超声心动

关键词： MDCT MR 动脉返流返流容积

A New Strategy for the Synthesis of Monomethylhydrazine Using the Raschig Process

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