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Osteogenic differentiation of encapsulated cells in dexamethasone-loaded phospholipid-induced silk fibroin hydrogels

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Biomaterials Translational 2022年第3期3卷 213-220页

作者： Chavee Laomeephol Helena Ferreira Sorada Kanokpanont Jittima Amie Luckanagul Nuno M neves Siriporn Damrongsakkul Department of Pharmaceutics and Industrial Pharmacy Faculty of Pharmaceutical SciencesChulalongkorn UniversityBangkokThailand Biomaterial Engineering for Medical and Health Research Unit Chulalongkorn UniversityBangkokThailand B’s Research Group I3Bs-Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineGuimarãesPortugal ICVS/3B’s-PT Government Associate Laboratory Braga/GuimarãesPortugal Department of Chemical Engineering Faculty of EngineeringChulalongkorn UniversityBangkokThailand Biomedical Engineering Research Center Faculty of EngineeringChulalongkorn UniversityBangkokThailand Center of Excellence in Plant-produced Pharmaceuticals Chulalongkorn UniversityBangkokThailand

The tissue engineering triad comprises the combination of cells,scaffolds and biological ***,we prepared cell-and drug-loaded hydrogels using in situ silk fibroin(SF)hydrogels induced by dimyristoyl glycerophosphoglycerol(DMPG).DMPG is reported to induce rapid hydrogel formation by SF,facilitating cell encapsulation in the hydrogel matrix while maintaining high cell viability and proliferative *** addition,DMPG can be used for liposome formulations in entrapping drug ***(Dex)was loaded into the DMPG-induced SF hydrogels together with human osteoblast-like SaOS-2 cells,then the osteogenic differentiation of the entrapped cells was evaluated in vitro and compared to cells cultured under standard *** production by cells cultured in DMPG/Dex-SF hydrogels with Dex-depleted osteogenic medium was equivalent to that of cells cultured in conventional osteogenic medium containing *** extended-release of the entrapped Dex by the hydrogels was able to provide a sufficient drug amount for osteogenic *** controlled release of Dex was also advantageous for cell viability even though its dose in the hydrogels was far higher than that in osteogenic *** results confirmed the possibility of using DMPG-induced SF hydrogels to enable dual cell and drug encapsulation to fulfil the practical applications of tissue-engineered constructs.

关键词： DMPG osteogenic differentiation silk fibroin three-dimensional cell culture tissue engineering

Arlenea delicata *** ***.,a new ephedroid plant from the Early Cretaceous Crato Formation,Araripe Basin,Northeast Brazil

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Plant Diversity 2024年第3期46卷 362-371页

作者： Alita Maria neves Ribeiro Yong Yang Antonio Alamo Feitosa Saraiva Renan Alfredo Machado Bantim Joao Tavares Calixto Junior Flaviana Jorge de Lima Programa de Pos-Graduaçao em Diversidade Biologica e Recursos Naturais Universidade Regional do CaririRua Carolino SucupiraPimenta63105-160CratoCearaBrazil Co-Innovation Center for Sustainable Forestry in Southern China College of Biology and the Environment159 Longpan RoadNanjing Forestry UniversityNanjing210037China Laboratorio de Paleontologia Universidade Regional do CaririRua Carolino SucupiraPimenta63105-160CratoCearaBrazil Laboratorio de Estudos da Flora Regional Universidade Regional do CaririRua Carolino SucupiraPimenta63105-160CratoCearaBrazil Gondwanan Plants Lab Universidade Federal de PernambucoCentro Academico de VitoriaRua Do Alto Reservatorio S/nBela Vista55608-680Vitoria de Santo AntaoPernambucoBrazil Programa de Pos-Graduaçao em Geociencias(PPGEOC) Universidade Federal de Pernambuco(UFPE)Rua Av.da ArquiteturaS/n°CEP-50740-550Cidade UniversitariaRecifePernambucoBrazil

Ephedroid macrofossils have been widely documented in Cretaceous deposits,including numerous from the Lower Cretaceous Yixian Formation of NE ***,few ephedroid macrofossils have been reported from South ***,we describe a new plant of the family Ephedraceae,Arlenea delicata *** ***.,from the Lower Cretaceous Crato Formation of the Araripe Basin,Northeast Brazil,based on the vegetative and reproductive *** has the typical morphological characteristics of ephedroid plants,including fertile reproductive branches,opposite phyllotaxy,terminal female cones,a sympodial branching system,longitudinally striated internodes,and swollen *** new finding is unusual in having inner chlamydosperms subtended by two pairs of bracts,reproductive units connected to branches through swollen receptacles and a smooth seed *** new ephedroid taxon from the Crato Formation increases our understanding of plant diversity of this group during the Early ***,the general morphology(fleshy bracts and enlarged receptacles)of this new fossil discovery indicates that seeds of this plant may have been dispersed by animals such as pterosaurs(mainly the Tapejaridae)and birds(Enantiornithes and Ornituromorpha).If true,this would explain the cosmopolitan distribution of Ephedraceae in the Lower Cretaceous.

关键词： Early Cretaceous Gnetophytes Arlenea delicata Ephedrales Crato Formation

Nanoparticle-encapsulated retinoic acid for the modulation of bone marrow hematopoietic stem cell niche

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Bioactive Materials 2024年第4期34卷 311-325页

作者： Emanuel Quartin Susana Rosa Sara Gonzalez-Anton Laura Mosteo Lopez Vitor Francisco Delfim Duarte Cristina Lo Celso Ricardo Pires das neves Lino Ferreira CNC-Center for Neuroscience and Cell Biology CIBB-Centre for Innovative Biomedicine and BiotechnologyUniversity of Coimbra3004-517CoimbraPortugal IIIUC-Institute of Interdisciplinary Research University of Coimbra3004-517CoimbraPortugal Faculty of Medicine University of Coimbra3004-517CoimbraPortugal Department of Life Sciences Imperial College LondonSouth Kensington CampusThe Francis Crick InstituteLondonUK Haematopoietic Stem Cell Laboratory The Francis Crick InstituteLondonUK Instituto de Investigação e Inovação em Saúde(i3S) University of PortoPortoPortugal Department of Biomedicine Faculdade de Medicina da Universidade do Porto(FMUP)PortoPortugal Department of Onco-Hematology Instituto Português de Oncologia(IPO)-PortoPortoPortugal

More effective approaches are needed in the treatment of blood cancers,in particular acute myeloid leukemia(AML),that are able to eliminate resistant leukemia stem cells(LSCs)at the bone marrow(BM),after a chemotherapy session,and then enhance hematopoietic stem cell(HSC)engraftment for the re-establishment of the HSC ***,we investigate whether light-activatable nanoparticles(NPs)encapsulating all-trans-retinoic acid(RA^(+)NPs)could solve both *** in vitro results show that mouse AML cells transfected with RA^(+)NPs differentiate towards antitumoral M1 macrophages through RIG.1 and OASL gene *** in vivo results further show that mouse AML cells transfected with RA^(+)NPs home at the BM after transplantation in an AML mouse *** photo-disassembly of the NPs within the grafted cells by a blue laser enables their differentiation towards a macrophage *** macrophage activation seems to have systemic anti-leukemic effect within the BM,with a significant reduction of leukemic cells in all BM compartments,of animals treated with RA^(+)NPs,when compared with animals treated with empty *** a separate group of experiments,we show for the first time that normal HSCs transfected with RA^(+)NPs show superior engraftment at the BM niche than cells without treatment or treated with empty *** is the first time that the activity of RA is tested in terms of long-term hematopoietic reconstitution after transplant using an in situ activation approach without any exogenous priming or genetic conditioning of the transplanted ***,the approach documented here has the potential to improve consolidation therapy in AML since it allows a dual intervention in the BM niche:to tackle resistant leukemia and improve HSC engraftment at the same time.

关键词： Light Nanoparticles Retinoic acid Leukemia M1 macrophages Drug delivery

Environmental and Durability Perspective of the Use of Curaua Fiber Treated in Mortars

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Journal of Renewable Materials 2022年第9期10卷 2409-2429页

作者： Afonso R.G.de Azevedo Marcelo Nascimento Dirlane do Carmo Markssuel T.Marvila Gustavo de C.Xavier Sergio neves Monteiro UENF-State University of the Northern Rio de Janeiro LECIV-Civil Engineering LaboratoryCampos dos GoytacazesBrazil UFF-Federal Fluminense University TER-Department of Agricultural EngineeringNiteróiBrazil UENF-State University of the Northern Rio de Janeiro LAMAV-Advanced Materials LaboratoryCampos dos GoytacazesBrazil IME-Military Institute of Engineering Department of Materials ScienceRio de JaneiroBrazil

The use of natural lignocellulosic fibers(NLFs)as a reinforcement mechanism for cementitious composites,such as mortar,has been investigated in the last ***,their application has often been restricted to technological evaluation research.A NLF with great potential the curaua,which after treatment with NaOH solution,proved to be technologically feasible for mortars reinforcement based on cement and *** objective of this research was the comparative evaluation between a traditional mortar,based on cement and lime,with 1:1:6:0.8 ratio of cement:lime:sand:water,and a modified mortar with addition of 2 wt.%treated curaua fiber in cement mass by evaluating environmental and durability *** a curing time for 28 days,environmental assessments were carried out and durability methodologies were *** tests performed were:(i)attack by chlorides and sulfate,(ii)the wetting and drying cycles,and(iii)slake durability *** results showed that the mortar with the addition of curaua fiber presented a similar behavior to the reference mixture,both in terms of environmental and durability *** modified mortar is able to be used in internal and external environments,the latter with some ***,it also contributes to the promotion of sustainable use of curaua fiber.

关键词： Natural lignocellulosic fibers curaua mortar durability environmental aspects

Stress, Executive Function, Resilience and Quality of Life in Portuguese Subjects in Situations of Economic Insufficiency and Unemployment

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Open Journal of Psychiatry 2015年第4期5卷 350-361页

作者： Eduardo Goncalves Saul neves de Jesus Department of Psychiatry and Mental Health of Hospital Center of Algarve Faro Portugal Department of Psychology of Faculty of Social and Human Sciences of University of Algarve Faro Portugal

The aim of this study is the investigation of the impact of stress of Portuguese subjects in situations of economic insufficiency and unemployment on executive function and quality of life and the coping strategies and resilience skills used. The sample consists of 41 participants. The psychometric instruments used are validated for Portuguese population, measure (perceived) stress, coping, material deprivation, resilience and quality of life, defined by World Health Organization. Executive function has been evaluated through performances at Stroop and Berg tasks. It has been concluded that, in this population, resilience skills and active coping strategies are positively correlated with quality of life. Quality of life is negatively correlated with material deprivation. Active coping strategies are supported by adequate executive function, which neurobiological substrate is dorso-lateral prefrontal cortex. Not active coping strategies correlate negatively with cognitive flexibility, suggesting the presence of a deficit at infero-lateral prefrontal cortex.

关键词： Stress Economic Insufficiency Unemployment Coping Resilience Quality of Life Executive Function

Vulnerability and Resilience to Stress and Immune and Neuroendocrine Function in Portuguese Subjects with Psychic Anomaly (Anxiety and Depression)

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Open Journal of Psychiatry 2015年第4期5卷 362-373页

The present study aimed to investigate the impact of chronic psychosocial stress and resilience, including at a biological level (immune and neuroendocrine function) in Portuguese citizens with psychic anomaly/mental disorder. The sample aggregated 69 participants. It has been used the following psychometric instruments: 21-item depression, anxiety and stress scales (DASS-21), in the Portuguese validated version;measuring state resilience (MSR), in the Portuguese validated version;the Portuguese scale of 23 questions on vulnerability to stress. Serum levels of cortisol, dehydroepiandrosterone sulfate, antibodies anti-viral capsid antigen of Epstein-Barr virus, triglycerides, high density lipoprotein-cholesterol and body mass index have been measured. It has been concluded that factors of vulnerability to stress and chronic stress, of social nature (lack of social support, adverse living conditions), correlate positively with depression, anxiety and stress, and, through alostatic load, are involved in a greater propensity for immune and neuroendocrine dysfunction in this population.

关键词： Vulnerability to Stress Stress Coping Resilience Alostatic Load Anxiety Depression Cortisol Dehydroepiandrosterone Epstein-Barr Virus Triglycerides High Density Lipoprotein-Cholesterol Body Mass Index

Study of Laboratory and Field Tests Related to Soil Compaction Based on Proficiency Testing Schemes

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材料科学与工程（中英文B版） 2013年第12期3卷 772-779页

作者： Jose neves Ana Duarte Claudia Silva Department of Civil Engineering Architecture and Georesources Instituto Superior Tecnico Universidade de Lisboa Lisboa1049-001 Portugal RELACRE Portuguese Association of Accredited Laboratories Lisboa 1050-113 Portugal

Distinct patterns of mucosal apoptosis in H pylori-associated gastric ulcer are associated with altered FasL and perforin cytotoxic pathways

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World Journal of Gastroenterology 2006年第38期12卷 6133-6141页

作者： Heitor SP Souza Marcelo S neves Celeste CS Elia Claudio JA Tortori Ilana Dines Cesonia A Martinusso Kalil Madi Leonardo Andrade Morgana TL Castelo-Branco Departamento de Clínica Médica Instituto de Ciências Biomédicas Universidade Federal do Rio de Janeiro 21941-590 Brazil Laboratório Multidisciplinar de Pesquisa e Departamento de Anatomia Patologica Instituto de Ciências Biomédicas Universidade Federal do Rio de Janeiro 21941-590 Brazil Hospital Universitário Clementino Fraga Filho Departamento de Histologia e Embriologia Instituto de Ciências Biomédicas Universidade Federal do Rio de Janeiro 21941-590 Brazil Laboratório de Imunologia Celular Instituto de Ciências Biomédicas Universidade Federal do Rio de Janeiro 21941-590 Brazil

AIM: To analyze the level of apoptosis in different mucosal compartments and the differential expression of Fas/Fas-ligand and perforin in H pylori-associated gastric ulcer. METHODS: Antral specimens from patients with H pylori-related active gastric ulcer (GU), H pylori-related gastritis, and non-infected controls were analysed for densities and distribution of apoptotic cells determined by the TdT-mediated dUDP-biotin nick-end-labelling method. GU patients were submitted to eradication therapy with follow-up biopsy after 60 d. Fas, FasL, and perforin-expressing cells were assessed by immunoper- oxidase, and with anti-CD3, anti-CD20 and anti-CD68 by double immunofluorescence and confocal microscopy. Quantitative analysis was performed using a computer- assisted image analyser. RESULTS: H pylori-infected antrum showed greater surface epithelial apoptosis which decreased after eradi- cation therapy. In the lamina propria, higher rates of mononuclear cell apoptosis were observed in H pylori- gastritis. Co-expression of Fas with T-cell and macro- phage markers was reduced in GU. FasL- and perforin-expressing cells were increased in H pylori-infection and correlated with epithelial apoptosis. Perforin-expressing cells were also increased in GU compared with H pylori- gastritis. CONCLUSION: Epithelial apoptosis is increased in H pylori-infection and correlates to FasL- and perforin- expression by T cells. Expression of perforin is correlated with the tissue damage, and may represent the enhance- ment of a distinct cytotoxic pathway in GU. Increased expression of FasL not paralleled by Fas on T-cells and macrophages may indicate a reduced susceptibility to the Fas/FasL-mediated apoptosis of lymphoid cells in H pylori-infection.

关键词： Gastric ulcer H pylori Apoptosis Fas receptor Fas ligand Perforin

The Connection of Solar Generators to the Electricity Distribution Network as a Means of Mitigating Environmental Impacts by Dispensing the Banks of Accumulators

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Energy and Power Engineering 2019年第12期11卷 392-397页

作者： Márcio Belloni Thadeu das neves Conti IPEN/USP S&atilde o Paulo Brazil

Solar generators are known worldwide as a means of generating clean electricity. However, the existence of battery banks causes alarm in the community by the existence of materials that degrade the environment by discarding them outside the norm. With the publication of Regulatory Standards 482/12 and 687/15, solar generators can be connected to the distribution grid, which serves as a means of accumulating the generated energy. The new changes were seen as an economic possibility, but there are more benefits. This has proved very important to make solar generation greener because it no longer needs large battery banks. This has also been shown to significantly lower the final value of the solar generator, bringing economic benefits. This paper aims to analyze the economic and environmental benefits of battery bank distribution in solar generation.

关键词： Solar Energy Decentralized Generation Battery Banks Renewable Energy